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Citations for Qiagen :
1601 - 1650 of 2825 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated for 1 hour with monoclonal α-His antibodies conjugated to the horseradish peroxidase (1:5000 dilution; Qiagen). Blots were visualized with the addition of Lumina Forte Western HRP Substrate (MilliporeSigma ...
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bioRxiv - Cell Biology 2024Quote: HeLa or HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, low glucose 1 g L−1, Qiagen #31885-023) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: ... was prepared by centrifuging a cell culture at 5525xg for 2 minutes at 4°C then resuspending cells in 1mL of PBS-RNAprotect (333 μL RNAprotect Bacteria Reagent [76506, Qiagen, Hilden, Germany], 666 μL PBS). For immediate RNA stabilization by RNAprotect (Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 30 mg of frozen tissue was placed into a 2 ml flat bottom centrifuge tube on dry ice with a 5 mm stainless steel bead (QIAGEN Germantown, MD, USA; Cat#69989) at the bottom ...
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bioRxiv - Neuroscience 2020Quote: ... along with the human NaV β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.X:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transiently co-transfected with NaV1.2 and the accessory β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.2:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Microbiology 2020Quote: Total RNA extraction was performed using a squash buffer (10 mM Tris base, 1 mM EDTA, 50 mM NaCl) supplemented with 1:8 part Proteinase K (Qiagen, 15mg/ml). Mosquito abdomens with the midguts and heads with thoraces were individually collected in 50 μl of squash buffer at 7 and 14 days ...
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bioRxiv - Microbiology 2022Quote: ... Total DNA (including integrated HIV-1 DNA and episomal HIV-1 DNA) was extracted using the QIAmp blood DNA minikit (Qiagen, Courtaboeuf, France) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... washed with 1 ml RNAprotect® Cell Reagent (Qiagen) and centrifuged for 10 min at 2,500 x g ...
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bioRxiv - Genomics 2020Quote: ... 5 µL Proteinase-K (20 mg ml-1, Qiagen) was added to the solution and incubated at 60°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µl zymolyase and 1 mg/ul RNase (QIAGEN). This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl ...
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bioRxiv - Physiology 2021Quote: 1 μg purified extracted RNA (RNeasy Mini Kit, Qiagen) was reverse transcribed using qScript Reverse Transcriptase (Quanta Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of proteinase K (20 mg/mL, Qiagen) was added and samples were incubated at RT for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-His6 (34650; IF: 1/500) from Qiagen, mAb anti-GAPDH (GTX28245 ...
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bioRxiv - Immunology 2020Quote: ... Samples were diluted at 4:1 (elution buffer (Qiagen)/cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... mouse antibodies against the His-tag (1:1000, Qiagen), rabbit antibodies against the C-terminal region of the collagen VI α1 chain (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Reaction conditions were 1 × HotStar® Taq buffer (Qiagen) supplemented with 1.6 mM MgCl2 ...
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bioRxiv - Neuroscience 2021Quote: ... and diluted 1:50 in TE-Buffer (Qiagen, #1018499) to a final concentration of 1.75e12 vg/ml (kindly donated by I ...
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bioRxiv - Immunology 2020Quote: ... HIV-1 RNA was isolated using the QIAcube (Qiagen) from mouse plasma using the QIAamp MinElute Virus Spin Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... 1:1,000 dilution of α-His-HRP antiserum (Qiagen), or 1:1,000 dilution of α-OmpA antiserum (Antibody Research Corporation ...
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bioRxiv - Genomics 2021Quote: ... then 24 mL buffer PB (5:1, Qiagen #19066) and 1.2mL NaOAc were added sequentially ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 U HotStar Taq (Qiagen; based on polymerization activity), and 32 U FEN1 (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA (1 μg) was treated with DNaseI (Qiagen). cDNA was generated using M-MLV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... treated with 1 mL of RNAprotect Bacteria Reagent (Qiagen), and pellets were stored at −80°C until RNA isolation ...
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bioRxiv - Genomics 2024Quote: ... whilst immersed in 1 mL QIAzol Lysis Reagent (QIAGEN). The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mL of Ni-NTA agarose resin (Qiagen, Germany) is packed onto a propylene chromatography/cartridge column (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... After adding 1 ml of buffer PB (QIAGEN recipe), the samples were purified using QIAquick spin columns (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... combined with 1:10 (v/v) proteinase K (Qiagen) treatment ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mL of Qiazol lysis reagent (Qiagen, #79306) and homogenized for 2 x 3 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of proteinase K (20 mg/mL, Qiagen) was added to these IP samples as well as the previously collected input samples ...
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bioRxiv - Genomics 2024Quote: ... 1% SDS with 15 uL Puregene Proteinase K (Qiagen)) at 55°C for 2 hours with inversion every 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... with miRNA Index Kit Version 1 (Qiagen, Cat: 331592) following the manufacturer’s instructions except that the amplifying PCR was conducted with 10-12 cycles ...
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bioRxiv - Genetics 2024Quote: ... followed by treatment with 1 μl DNase I (Qiagen). After quality control assessments ...
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bioRxiv - Genomics 2021Quote: Amplicon libraries for viral genome sequencing were prepared using QIAseq FX DNA Library Kit and QIAseq SARS-CoV-2 Primer Panel (Qiagen, cat. no. 180475, cat. no. 333896) as instructed by the manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2024Quote: ... An aliquot of 10% unlysed cells per flask were set aside for later plasmid extraction (pelleted and stored at –20C) and the remaining cells were pelleted and lysed in 2 ml RLT (Qiagen RNeasy Midi Kit cat. no. 75144) plus 20 μl 2-mercaptoethanol (Sigma cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspend the cells in 10 ml of suspension buffer (10 mM EDTA pH8.0, 150 mM NaCl, 1% glycerol, Lysis blue (1×, from QIAGEN Plasmid Plus Midi Kit), RNase A (0.55 mg/ml) ...
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bioRxiv - Microbiology 2020Quote: ... into 1 ml of RNA Protect solution (Qiagen, Hilden, Germany)
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bioRxiv - Biochemistry 2022Quote: ... and incubated with 1 ml of Ni-Sepharose beads (Qiagen) on a rotator for 1 hour at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... cells were resuspended in 1 ml buffer RLT from Qiagen RNeasy kit ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatants were digested in 1 mg/ml Proteinase K (Qiagen) for 12 h at 55 °C then boiled for 10 min to inactivate the enzyme ...
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bioRxiv - Cell Biology 2021Quote: ... Validated qPCR primers and primer assays (Qiagen, see Table 1) were used together with QuantiTect SYBR Green PCR kit (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... approximately 100 mg was stored in 1 ml RNAlater (Qiagen) for at least 4 days for stabilization ...
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bioRxiv - Microbiology 2021Quote: ... was packed with 1 mL of Ni-NTA superflow (Qiagen). After washing with 10 mL of MilliQ water ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Penta-His-biotin conjugate 1:5000 (Qiagen) overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in 1 mL RLT Buffer (Qiagen 79216) and vortexed for 1 minute ...
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bioRxiv - Genomics 2020Quote: ... 1 μl of diluted (0.08x) FastSelect rRNA HMR (Qiagen, Germany) and 1 μl of diluted (0.08x ...
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bioRxiv - Genomics 2020Quote: ... 1 μl of diluted (0.08x) FastSelect rRNA HMR (Qiagen, Germany) was added into 6 μl COVID-19 specimen RNA along with 3 μl NA denaturation buffer ...
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bioRxiv - Microbiology 2020Quote: ... 1 µg/ µl BSA and 10X HotStartTaq Master Mix (Qiagen). Cycling conditions included initial denaturation at 95°C for 15 min ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and 1 mL of QIAzol lysis reagent (Qiagen, item #79306). Tissue homogenization was done by agitating the tubes for 20□s at a 7□m□s−□1 speed (Beadbug 6 homogenizer ...
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bioRxiv - Microbiology 2021Quote: ... Tris 10 mM) supplemented with 1% Proteinase K (Qiagen #19133) and 1% RNAse A/T1 (ThermoFisher #EN0551 ...