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Citations for Qiagen :
1551 - 1600 of 2825 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The restriction digestion efficiency was validated using 1% 1× TBE agarose gel electrophoresis and purified with a QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... homogenized in 1 ml PBS by bead beating (3mm steel ball, 25 Hz for 1 minute in a TissueLyser (Qiagen)) ...
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bioRxiv - Genomics 2021Quote: ... 1 g of ground leaf powder was resuspended in lysis buffer with 1 mg/ml of proteinase K (19133, Qiagen) and 190 μg/ml of RNase A (19101 ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from 1×106 MCF7 and 1×106 BT-474 cells (‘bulk’) using the RNeasy Mini Kit (74104, Qiagen) in combination with the QIAshredder (79654 ...
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bioRxiv - Immunology 2022Quote: ... For bacterial load determination one lobe of the lung was collected in 1 mL PBS with 1 metal bead and smashed using a tissue lyser (Qiagen). 25 μL of lysed lung were put in duplicates on CYE-plates and grown for 3-4 days at 37°C in a non-CO2 incubator.
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bioRxiv - Physiology 2024Quote: ... After chloroform incubation and centrifugation per manufacture instructions RNA-containing phase was diluted with 70% ethanol 1:1 and RNA isolation was performed using RNeasy Mini Kit (Qiagen). For tissues ...
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bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was mixed 1:1 with 70% ethanol and applied to a minikit spin column and washed with RW1 and RPE buffer (Qiagen). cDNA was synthesized using the QuantiTect Rev ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 1:1 of 70% ethanol was mixed with the upper phase before transfer on column (RNeasy Mini kit, Qiagen), and RNA was extracted according to manufacturer directions ...
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bioRxiv - Plant Biology 2023Quote: ... of g1090::XVE>>AXR3-1-mCherry and HS::AXR3-1 were extracted following the protocol (Plant Total RNA Mini Kit, QIAGEN). g1090::XVE>>AXR3-1-mCherry were transferred for 2 h on estradiol-containing medium and then harvested ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Bioengineering 2022Quote: ... the aqueous phase was mixed with ethanol in a 1:1 ratio and was added to RNeasy spin columns (Qiagen) for RNA purification ...
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bioRxiv - Cancer Biology 2023Quote: ... Digested PCR fragments were ligated into the restricted vector at a 1:1 molar ratio using T4 DNA ligase and were purified using the MiniElute PCR purification kit (Qiagen). Purified ligated plasmids were electroporated into MegaX DH10B (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... was added to the supernatant to give a final concentration of 1 mM and the resultant suspension was applied to a 1 ml Ni2+-NTA column (Qiagen). The resin was then washed sequentially with 10 ml of lysis buffer (without Triton X-100) ...
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bioRxiv - Biophysics 2024Quote: ... The significantly differentially expressed genes with a log2 fold change <= −1 or >= 1 were input into Ingenuity Pathway Analysis (Qiagen) to determine pathway enrichment (significance determined by Fischer’s exact method).
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bioRxiv - Genomics 2024Quote: ... which was pre-wet with 1 mL cold buffer 1 before it was placed on the receiving plate attached to a QIAvac96 (Qiagen). Pressure was maintained below 100 bar during filtration ...
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bioRxiv - Molecular Biology 2024Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting supernatant was then collected and filtered through miracloth before a 1 hr incubation with 1 mL of Ni-NTA agarose (Qiagen). Matrix was washed with buffer A (50 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from mouse (NPsh-1-IDH2WT, NPsh-1-IDH2R172K) and human cells (RBE) with the RNAeasy extraction kit (Qiagen). Bulk RNA-SEQ of NPsh-1-IDH2WT ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in Propidium Iodide (PI) staining solution (1x PBS supplemented with 50 µg/mL PI, Invitrogen; 100 µg/mL RNase A, Qiagen; and 2 mM MgCl2) and incubated for 30 min at room temperature in the dark) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 ng of pFLAG expression vectors or empty plasmids and 50 ng psiCheck-2 plasmid were transfected using Effectene transfection reagent (Qiagen, Netherlands, Cat. No. 301425), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 80 µl of Viral Transport Media that had previously stored a nasopharyngeal swab from a patient infected with SARS-CoV-2 were used for RNA isolation using the QIAamp Viral RNA Mini spin kit (Qiagen, Cat No./ID: 52904) according to manufacturer specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Both the input and IP samples were incubated at 50°C for 2 hr and then cleaned up using the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany, Cat # 28104) and eluted in 35 μL of water.
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the remaining 2 mL of the cell suspension using the RNeasy® Protect Bacteria Mini Kit (QIAGEN, Cat. No. 74524) following the manufacturer instructions.
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from bulk HSPC samples (∼2 x 106 cells per sample) using a QIAGEN Blood & Tissue DNA isolation kit (QIAGEN, Inc., Germantown, MD, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were digested for 60 minutes with proteinase K (10 µl) followed by bead-beating for 40 minutes (Vortex-Genie 2 with vortex adapter, Qiagen, Cat: #13000-V1-24) at maximal speed ...
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bioRxiv - Immunology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... and 0.5 mg.ml-1 of Proteinase K (Qiagen), to reverse formaldehyde crosslinking ...
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bioRxiv - Microbiology 2021Quote: ... and 1-5mL Qiazol Lysis Reagent (Qiagen, #79306) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... amplification grade (1 U/µg RNA, Qiagen, #79254). Real-time qPCR was performed with a MyiQ™ instrument (BIO-RAD) ...
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bioRxiv - Biochemistry 2022Quote: ... Penta-His Antibody (Qiagen, 34660, IB: 1:1000) and Y188 to APP C-terminus (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... passed over 1 mL Ni-NTA resin (Qiagen), and bound protein eluted with 10 CV of buffer A / 200 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Genetics 2020Quote: ... 1× Multiplex PCR Master Mix (Qiagen, Hilden, Germany), and 0.2 μM of Primer_F3 and Primer_R (Figure S2 and Table S2) ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen):cDNA) ...
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bioRxiv - Genomics 2022Quote: ... 1 mL of buffer PB or PM (Qiagen), containing a high concentration of guanidine hydrochloride and isopropanol was added and mixed by pipetting ...
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bioRxiv - Physiology 2022Quote: ... Samples were homogenized in 1 ml Qiazol (Qiagen) with 5 μl β-mercaptoethanol using a beadmill homogenizer cooled with liquid nitrogen and then phase separated using 1-bromo-3-chloropropane (BCP) ...
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bioRxiv - Biochemistry 2024Quote: ... and anti-Strep (Qiagen, 34850, 1:1000 dilution) antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Proteinase K (20 μg·ml-1 final concentration, Qiagen) was firstly added to the relevant samples ...
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bioRxiv - Biochemistry 2024Quote: ... and 1% Protease Inhibitor using TissueLyser II (Qiagen) and 5 mm stainless steel beads (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen): cDNA ...
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bioRxiv - Biophysics 2023Quote: ... Approximately 1 ml of Ni-NTA agarose (Qiagen) was utilised for purification of the lysate resulting from 1 l culture ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mL of RNA protect reagent (76506, Qiagen) was added to 500 µL of the sample ...
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bioRxiv - Systems Biology 2023Quote: ... 1 mL of ice-cold Qiazol (Qiagen, 79306) reagent was added and transferred into Precellys® lysing kit tubes (Keramik-kit 1.4/2.8 mm ...
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bioRxiv - Physiology 2024Quote: ... and QuantiTect primer assays (QIAGEN; Suppl. Table 1). Expression was determined in triplicate from each biological replicate ...
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bioRxiv - Immunology 2024Quote: ... Samples were diluted 9:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 1 mL of inhibitEX buffer (Qiagen), and transferred to 2 mL tubes containing 0.5 g of 0.1 mm zirconium beads (Biospec) ...
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bioRxiv - Molecular Biology 2022Quote: ... FAF1 (Uniprot identifier Q9UNN5-1) and UBXN7 (Uniprot identifier O94888-1) were amplified from XpressRef Universal Total human RNA (QIAGEN, 338112) by RT-PCR (TaKaRa ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted with 1 volume of 25:24:1 phenol-chloroform-isoamyl alcohol and purified with QIAquick PCR Purification Kit (QIAGEN 28104). Libraries were prepared with the Ovation Ultralow V2 DNA-Seq Library Preparation Kit (NuGEN (Redwood City ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...