Labshake search
Citations for Qiagen :
1551 - 1600 of 3415 citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... OR) and total RNA was isolated using QIAzol Lysis Reagent and the RNeasy Lipid Tissue Mini Kit (Qiagen, Valencia, CA). The concentration and purity were determined using NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... These plasmids were purified and DNA extracted using QIAGEN Plasmid Midi Kit (100) (cat. n° 12145, QIAGEN, Valencia, CA, USA), according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from the samples using either the Qiagen (Valencia, CA, USA) DNeasy Blood & Tissue kit or the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s recommendations as outlined in Lee et al ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Genomic DNA from whole louse specimens was individually extracted using the Qiagen QIAamp DNA Micro Kit (Qiagen, Valencia, CA, USA). Membership to individual louse lineages (SE ...
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bioRxiv - Microbiology 2021Quote: ... in Tris/HCl/Ca+2 buffer was subjected to extraction in house using the silica method or QIAamp viral RNA mini kit (Qiagen) according to the manufacturer’s guidelines.
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bioRxiv - Microbiology 2021Quote: ... and on-column DNA digestion was performed using Qiagen RNase-Free DNase Set according to manufacturer’s protocol (Qiagen, Valencia, CA). The RNA purity was tested by measuring 260 nm/280 nm and 260 nm/230 nm ratios in TE buffer and the values should be over 1.8 ...
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bioRxiv - Microbiology 2023Quote: ... Tissue and skeleton samples were then preserved in PowerSoil DNA Isolation kit (MoBio Laboratories, Carlsbad, CA; now Qiagen, Venlo, Netherlands) bead tubes ...
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bioRxiv - Microbiology 2023Quote: Bacterial and archaeal DNA were extracted using the PowerSoil DNA Isolation Kit (MoBio Laboratories, Carlsbad, CA; now Qiagen, Venlo, Netherlands). To select for the 16S rRNA V4 gene region ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from homogenized ileal and colonic tissues by use of the RNeasy Mini kit (Qiagen, Valencia, CA) and reverse transcribed (High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA and RNA were isolated simultaneously from each section with the AllPrep DNA / RNA / miRNA kit (Qiagen Inc., Valencia, CA). All nucleic acid isolation from tissue sections was performed using a QIAcube automated sample preparation system according to the manufacturer’s instructions (Qiagen Inc. ...
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bioRxiv - Bioengineering 2024Quote: ... Plasmid DNA for plastid transformation was prepared using the QIAGEN Plasmid Maxi Kit (Cat. No 12162; Qiagen inc., Valencia, CA). Leaves were placed abaxial side up on the RMOP medium ...
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bioRxiv - Evolutionary Biology 2024Quote: We extracted DNA from the homogenized right-side of all embryos using a Gentra PureGene kit (Qiagen, Valencia, CA, USA) following manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: Pools of 5 embryos were used for RNA extraction via the Qiagen RNeasy Micro Kit (Qiagen, Valencia CA; Cat# 74004). RNA was eluted in RNase-free water and sent to the New York University Genome Technology Center for RNA-sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... and RNA was isolated for all study participants in parallel using an RNAeasy Micro Kit (Cat #74004, Qiagen, Valencia, CA). Extracted RNA was quantified with a Qubit fluorometer (Cat #Q33238 ...
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bioRxiv - Genomics 2024Quote: The DNA of the 80 used for GBS was isolated using the spin-column DNeasy plant mini kit (Qiagen USA, Valencia, CA) (Qiagen). For the other 259 samples ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from mixed stage growing populations using reagents from the Puregene Core Kit A (QIAGEN, Valencia, CA) and quantified using Qubit 3.0 fluorometer (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum 3D7 ring stage parasites were transfected with 100 μg of purified plasmid DNA (Plasmid Maxi Kit, Qiagen, Valencia, CA) by electroporation (310 V ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from TSB cultures following the manufacturer’s instructions using the DNeasy Blood and Tissue kit (Qiagen, Carlsbad, CA). Purified DNA was quantified ...
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bioRxiv - Genomics 2023Quote: ... genomic DNA samples were obtained from peripheral blood of healthy study participants using QIAamp DNA Blood Mini Kits (QIAGEN, CA) in accordance with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from the lysate per manufacturer instructions using a AllPrep DNA/RNA mini kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Immunology 2023Quote: ... A 0.3 ml portion of this homogenate was used to extract and purify RNA using the RNeasy Mini Kit (CAS: 74104, Qiagen) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA extraction from thallus tissue (ca. 100mg including apical notch) was done using the Rneasy plant mini kit (#74904, Qiagen). To remove contaminating DNA ...
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bioRxiv - Genomics 2022Quote: ... MALRYJM145 was PCR-amplified from YJM145 genomic DNA isolated using a DNeasy blood and tissue kit (Qiagen, Valencia, CA, USA). The PCR used primers oMS90/111 ...
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bioRxiv - Genetics 2022Quote: ... 8 cm of leaf tissue was harvested on ice and then lyophilized using a TissueLyser II (Qiagen, Valencia, CA, USA). Genomic DNA was extracted using a Qiagen DNeasy Plant Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... individually crushed and lysed with the help of Mini-Beadbeater-16 from BioSpec using sterile zirconia beads and the DNA was purified using the DNAeasy® blood and Tissue kit (Qiagen, Valencia CA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the large stocks of recombinant T/F LTR-pGL3 reporter plasmids were generated using Plasmid Maxi kit (Qiagen, Valencia, CA) for immediate use or longer storage at -80 °C.
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bioRxiv - Microbiology 2023Quote: ... then separation of DNA and RNA was completed using the AllPrep DNA/RNA Mini Kit (Qiagen Inc, Valencia, CA, USA). The amount of DNA or RNA extracted was quantified using the Qubit 1X dsDNA Broad Range Kit or Qubit RNA High Sensitivity Kit ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA was extracted from the 3D or 2D cultures using the RNeasy Total RNA kit (Qiagen, Valencia, CA, USA). The cDNA synthesis and RT-qPCR steps were performed simultaneously using the pPCRBIO Probe 1-Step Go kit (PCR Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... Bacterial and archaeal DNA were extracted using the PowerSoil DNA Isolation Kit (MoBio Laboratories, Carlsbad, CA; now Qiagen, Venlo, Netherlands). To select for the 16S rRNA V4 gene region ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA brain tissue punches were homogenized in 96-well plate format on a QIAGEN TissueLyser II (Qiagen, Valencia, CA; #85300) in 600 μL of homogenizing buffer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from lyophilized mycelium of isolate 1987 with the DNeasy® Plant Mini Kit (QIAGEN, Valencia, CA, USA) and used as template in a PCR to rule out the integration of one movement protein (MP ...
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bioRxiv - Physiology 2024Quote: ... RNA extraction was performed using TRIzol and isolated total RNA was purified using a RNeasy Mini kit (Qiagen, Valencia, CA). The resulting quantity and purity of total RNA was tested through absorbance spectrophotometry at 230 ...
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bioRxiv - Physiology 2024Quote: ... Liver RNA was isolated from ∼ 25 mg of liver tissue using an RNeasy Plus Mini Kit (Qiagen, Valencia, CA, USA) and hypothalamus RNA was isolated using the standard Triazol procedure ...
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bioRxiv - Biochemistry 2024Quote: ... Enriched diseases and/or biological functions analysis were performed by Ingenuity Pathway Analysis (IPA) software (QIAGEN Bioinformatics, Redwood City, CA). Differentially expressed genes from [4 comparison groups] were uploaded into IPA ...
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bioRxiv - Bioengineering 2024Quote: ... total DNA of cultured cells or spleen cells was isolated using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA). A spike-in sequence was added prior to DNA extraction at the same concentration in each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA was extracted from ethanol-preserved tissue using the Qiagen DNEasy Blood and Tissue Kit (Qiagen, Redwood City, CA). Illumina library preparation and sequencing (150bp ...
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bioRxiv - Biophysics 2020Quote: ... or DNAse (200 Units well−1, 1 hr, 37°C, Qiagen). DAPI was stained at 0.5 μM (5 mins ...
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bioRxiv - Immunology 2022Quote: ... Virus was inactivated by mixing 1:1 with Buffer ATL (Qiagen) prior to viral RNA extraction from NP swabs ...
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bioRxiv - Developmental Biology 2021Quote: ... Luciferase reporter constructs were either mock-treated or methylated in vitro with SssI CpG methyltransferase for 4 h at 37 °C and purified with the QIAquick Purification Kit (QIAGEN 28704). Reporter plasmid (500 ng ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of plasmid DNA was transfected into each well using Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427). Unless otherwise noted ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was ground to small pieces by one steel ball (Ø 4 mm) in a 2 ml Eppendorf tube with Tissue Lyzer II (Qiagen, Hilden ...
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bioRxiv - Biochemistry 2020Quote: ... 4°C) an applied to a 2 ml Ni-NTA immobilised metal affinity chromatography (IMAC) gravity flow column (QIAGEN, Hilden, Germany). Columns were washed in 10 column volumes (CV ...
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bioRxiv - Molecular Biology 2021Quote: ... the PCR products were held at 4°C until PCR purification using the QIAquick PCR purification kit (#28106, Qiagen, Hilden, Germany). DNA concentrations were determined using NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 150 mg of cluster roots (3 to 4 roots) using the RNeasy Plant Mini Kit (Qiagen, 74904) and treated with the DNA-free DNA Removal Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 100μL chloroform were added and samples spun at 7000rpm for 15min at +4°C in a MaXtract column (Qiagen, Venlo, Netherlands). Mixed with 500μL 70% EtOH and spun at 10 000rpm for 30s at +4°C in a MiniElute column ...
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bioRxiv - Genomics 2022Quote: ... Day 145) for n=4 clams for each treatment (Figure 2) using the Qiagen DNeasy Blood and Tissue Kit (Qiagen USA) according to manufacturer’s instructions with slight modifications ...
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bioRxiv - Neuroscience 2022Quote: At DIV 28-30 iPSC-MG from 8 C9orf72 ALS/FTD patient lines and 4 control lines were pelleted and lysed using QIAshredder (QIAGEN-79654) and RNA was isolated with RNeasy Mini Kit (QIAGEN-74104 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN, Germany) equilibrated with purification buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...