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Citations for Qiagen :
1351 - 1400 of 3415 citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble lysate obtained after centrifugation at 13,000 rpm for 30 minutes at 4°C was incubated with Ni-NTA agarose resin (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from HAEC and HCAEC (from passages 4-5) using RNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Clarified cell lysates were centrifuged at 30,000 g for 30 min at 4°C before purification using Ni-NTA resin (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was clarified by centrifugation (35,000xg, 45 mins at 4℃) and the supernatant incubated with Ni-NTA resin (Qiagen) for 1 hour at 4℃ ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 92,600 x g for 30 min at 4 °C and incubated with Ni-NTA agarose beads (Qiagen) for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted from three independent biological replicates for each genotype using RNeasy Plant Mini Kits (Qiagen, CA). Genomic DNA contamination was removed by digestion with Turbo DNase (BioRad ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA from melanoma cells and freshly frozen tumors was extracted with the DNeasy purification kit (Qiagen Inc., Valencia, CA). High melanin content was removed with OneStep™ PCR Inhibitor Removal Kit (Zymo Research Corporation ...
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bioRxiv - Genomics 2021Quote: Functional analysis of the differentially expressed genes was conducted using Ingenuity Pathway Analysis (IPA, Qiagen, Redwood City, CA, USA) using probes with FDR-corrected p-value < 0.05 ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was obtained from the frozen bacteria pellets using a DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... High molecular weight DNA was isolated from each coral tissue sample using the Qiagen DNeasy kit (Qiagen, Valencia, CA) without prior enrichment for S ...
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bioRxiv - Microbiology 2021Quote: ... Cln1-His6 and its associated proteins were co-purified following the Ni-NTA Spin Kit procedure (Qiagen, Valencia, CA). Cell lysate from the CLN1-His6 strain was used as a negative control ...
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bioRxiv - Neuroscience 2020Quote: Each DNA sample (~100 μl of fecal DNA) was extracted using the DNeasy Blood & Tissue kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... total RNA was extracted from purified splenic T-lymphocytes utilizing the RNAeasy mini kit (Qiagen #74104, Valencia, CA, USA) prior to conversion to cDNA (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... De-paraffinization of FFPE tumor and adjacent normal specimens was performed using QIAGEN de-paraffinization solution (QIAGEN, Valencia, CA). DNA and RNA from 19 tumor and adjacent normal samples was extracted using the AllPrep DNA/RNA FFPE Kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: Total DNA was extracted from cecal contents using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Valencia, CA, USA) with an additional bead-beating step with ~200 mg of garnet beads at 6.0 m/s for 60 s (FastPrep-24 5G instrument ...
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bioRxiv - Microbiology 2021Quote: ... Target bands (∼420 bp) were cut and eluted using a QIAEX II gel extraction kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Microbiology 2021Quote: RNA and DNA were extracted using RNeasy PowerSoil Total RNA and DNeasy PowerSoil Pro kits (Qiagen, Valencia, CA, USA), respectively ...
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bioRxiv - Microbiology 2020Quote: ... and all of them were diluted to 5ng/μl by using Microbial DNA-Free Water (Qiagen, Valencia, CA, USA). The second-round PCR ...
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bioRxiv - Microbiology 2020Quote: ... The cleaned-up DNA was the gel-purified by using the QIAquick Gel Extraction Kit (Qiagen, Valencia, CA, USA) and then quantified by using Qubit® (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Total RNA was isolated from cultured macrophages or skin biopsies using lysis buffer (RLT; Qiagen, Redwood City, CA, USA). The RT2 First Strand Kit reverse transcription system (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from whole embryonic lungs at E18.5 using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was then isolated using the miRNeasy mini columns as described by the manufacturers’ protocol (Qiagen; Valencia, CA, USA). Following elution from the miRNeasy column ...
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bioRxiv - Immunology 2022Quote: ... cDNA was prepared from 0.5μg RNA used as template with the RT2 First Strand kit (Qiagen Inc, Valencia, CA). Gene expression analysis of ROS associated genes (n=84 ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA from each of the isolates was extracted using the Dneasy UltraClean Microbial Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... PCR product was pooled and purified two times with a QIAquick PCR Purification Kit (Qiagen, Inc., Valencia, CA, USA) and DNA quantity was measured on an Agilent 2200 TapeStation (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from the samples using the Qiagen DNeasy Blood & Tissue Mini kit (Qiagen, Valencia, CA, USA). The concentration and purity of extracted genomic DNA samples were evaluated using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Genetics 2020Quote: ... DNA extraction was performed using the Qiagen DNEasy Plant Mini Kit following the manufacturer’s protocol (QIAGEN, Valencia, CA, USA). Concentration and purity of extracted DNA was evaluated using a Nanodrop spectrophotometer (NanoDrop Technologies Inc. ...
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bioRxiv - Microbiology 2020Quote: Cells were harvested 24 h after treatment and RNA was extracted using the RNeasy 96 protocol (Qiagen, Valencia, CA). Gene expression for 22,238 probe sets of 12,490 genes was generated with U133A2.0 microarrays following the manufacturer’s instructions (Affymetrix ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Tissue was homogenized and RNA was extracted using the RNeasy® Plus Universal Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturers’ guidelines for the isolation of total RNA including small RNAs ...
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bioRxiv - Plant Biology 2020Quote: ... the primary reads were aligned to the Arabidopsis chloroplast genome (accession number NC_000932.1) using CLC Genomics Workbench 6.5.1 (Qiagen, Valencia, CA, USA) with the following parameters ...
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bioRxiv - Genomics 2020Quote: DNA for methylation studies was extracted from thawed CBMCs and PBMCs using the Qiagen AllPrep kit (QIAGEN, Valencia, CA). Genome-wide DNA methylation was assessed using the Illumina Infinium MethylationEPIC BeadChip (Illumina ...
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bioRxiv - Genomics 2021Quote: DNA was extracted using the Qiagen® Genomic DNA Kit following the manufacturer guidelines (Cat#13323, Qiagen, Valencia, CA). Quality and quantity of total DNA were evaluated using a NanoDrop™One UV-Vis spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from each LEC sample obtained from SCR was extracted using the miRNeasy Micro Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C and genomic DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). The concentration of resultant DNA was determined using a Qubit double-stranded DNA BR assay kit and a Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from all samples by following the Qiagen DNeasy Powersoil Pro Kit protocol (Qiagen; Valencia, CA, USA). Bead-beating was performed with a Mini-BeadBeater (BioSpec ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from aphid samples using a DNeasy Blood & Tissue Extraction Kit (Qiagen Inc., Valencia, CA, USA) by following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Genes with called mutations were analyzed for functional relationships with the Ingenuity Pathway Analysis product (QIAGEN, Valencia, CA, USA), using the Path Explorer option of the Path Designer tool ...
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bioRxiv - Microbiology 2022Quote: ... adaptor trimming and de novo transcriptome assembly were carried out using CLC Genomics Workbench (Qiagen, Redwood City, CA, USA). A reference transcriptome was generated by a combined assembly of both hindgut wall and pocket reads using CLC Genomics Workbench v10.1 software with standard settings and an additional CLC-based assembly with the following parameters ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from the lungs using TRIzol reagent and purified by RNeasy mini columns (Qiagen, CA, USA). The eluted RNA was subjected to complementary DNA synthesis using a High-Capacity cDNA Reverse Transcription Kit as per the suggested protocol (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Viral genomic DNA was purified from the harvested cells using a QIAamp DNA Blood mini kit (Qiagen, CA, USA). The viral genome copy number was quantified by qPCR and normalized to the amount of total DNA.
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bioRxiv - Developmental Biology 2023Quote: ... The RNA treated with RNase-free DNase was cleaned up using the RNeasy Mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: RNA from the collar cells of regenerating feather buds was isolated using the Qiagen RNEasy Kit (Qiagen, Valencia CA), treated with the Zymo PCR Inhibitor Removal kit (Zymo Research ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was retro-transcribed using a cDNA synthesis kit (Bio-Rad, Hercules, CA, USA or Qiagen, Hilden, Germany) and transcript levels were determined by RT-qPCR using the SsoAdvanced SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... cfDNA was extracted from 0.5 ml of plasma or serum using the QIAmp DNA Mini Blood kit (Qiagen, CA), according to the ‘‘Blood and body fluid protocol’’ and our own detailed protocol [37] ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by cell lysis and purification using immobilized metal-affinity chromatography (IMAC) in a Ni–NTA (Qiagen, Valencia, CA) column in binding buffer composed of 20 mM HEPES pH 7.25 ...
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bioRxiv - Genomics 2023Quote: ... We extracted total RNA from most adult tissues (minus the brain) using the RNeasy Kit (QIAGEN, Redwood City, CA) and submitted the extracted total RNA to Novogene Ltd ...
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bioRxiv - Molecular Biology 2024Quote: ... we sequenced the rbcL F3R3 region of 104 predominant plant species at the study site using the following methods. Total DNA was extracted from plant specimens (ca. 0.8 cm2) using a DNeasy Plant Mini Kit (Qiagen) and purified using a Geneclean Spin Kit (MP-Biomedicals) ...
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bioRxiv - Immunology 2024Quote: ... DNA from the blood of Plasmodium chabaudi infected mice was isolated from QIAmp DNA blood kit (QIAGEN, Valencia, CA) as per the manufacturer instruction ...
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bioRxiv - Microbiology 2023Quote: ... DNA from approximately 1g of surface sterilized seeds were isolated using DNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA) with necessary modifications ...