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Citations for Qiagen :
1501 - 1550 of 2378 citations for 6H 5 Oxa 1 2a 4a triazacyclopenta cd pentalene 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... run on a 1 % agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). tdTomato and neomycin were cloned out from H3.1-miCOUNT (Denoth-Lippuner et al. ...
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bioRxiv - Cell Biology 2022Quote: ... run on a 1 % agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). The plasmid backbone ...
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bioRxiv - Bioengineering 2020Quote: ... Each well is then diluted with 1 to 4 v:v in RNAse free elution buffer (QIAgen) to a total volume of 8 µL ...
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bioRxiv - Immunology 2021Quote: DNA from 1-2 stool pellets was extracted using the QIAamp DNA Stool Mini Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and 1 μg of linearized DNA was transfected into A549 cells using Effectene Transfection Reagent (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Cultures samples were then mixed 1:2 with RNA Protect Bacteria Reagent (QIAGEN, Germantown, Maryland, USA), vortexed immediately for 5 seconds and incubated for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... pyrosequencing was conducted on a PyroMark Q96 MD instrument using CpG LINE-1 assay (973043, Qiagen). To monitor bisulfite conversion efficiency ...
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bioRxiv - Immunology 2022Quote: ... freshly sorted G1-ILC populations (1 × 103 cells) were lysed with Buffer RLT (Qiagen, Hilden, Germany) and purified with RNAClean XP (Beckman Coulter ...
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bioRxiv - Physiology 2022Quote: ... was extracted from adipose tissue (experiment 1) using the Dneasy Blood and Tissue Kit (Qiagen, # 69504). We amplified both the mouse genomic gene Glyceraldehyde 3-phosphate dehydrogenase (Gapdh ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was collected and tumbled with 1 mL of preequilibrated Ni(II)-NTA Superflow (Qiagen) resin for 1-1.5 h at 4 °C.
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were purified on a 1% gel and extracted using QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNAs were prepared in RLT RNA lysis buffer supplemented with 1:100 β-mercaptoethanol (Qiagen) at 5,000 cells/µl ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1 mg of RNA was reverse transcribed to cDNA using the Omniscript RT Kit (Qiagen). qPCR was performed using the TaqMan assay system ...
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bioRxiv - Immunology 2020Quote: ... Disruption of microbial cells was enhanced using TissueLyser II (QIAGEN, 30 shakes/sec., 1 min., 2x). To lyse Gram-negative bacteria ...
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bioRxiv - Microbiology 2020Quote: ... 1 ug of RNA was reverse transcribed to cDNA with the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 mL of each culture was mixed with 1 mL of RNA Bacteria Protect Reagent (Qiagen) immediately after 2 mM ZnCl2 induction (t0 ...
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bioRxiv - Genomics 2021Quote: ... size-selected on a 1% agarose gel and purified using the MinElute PCR Purification Kit (Qiagen). The DNA library was then cloned into the vector using four In-Fusion cloning reactions according to the manufacturer’s instructions (Clontech In-Fusion HD) ...
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bioRxiv - Genomics 2021Quote: ... size-selected on a 1% agarose gel and extracted with the QIAquick Gel Extraction Kit (Qiagen). The eluates were pooled and purified using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: Genomic DNA from 1 g leaf material was isolated using the DNeasy Plant Maxi kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... RNA was extracted from THP-1 and cDNA was synthesized using RT2 First strand kit (Qiagen). Pre-designed RT2 qPCR primer assays (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 ml of PE buffer and 100 μl of EB buffer (MinElute PCR Purification Kit, QIAGEN).
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bioRxiv - Genetics 2021Quote: ... Replicates were lysed in 1 mL of RLT buffer and homogenized using QIAshredder columns (Qiagen, 79654). Total RNA was extracted and eluted in 100 uL RNase-free water using an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL for OD600 ~0.4 for 10% CO2) and stabilized by RNA Protect Bacteria Kit (Qiagen). Next ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA from A549 cells (1 × 106 cells) was extracted using miRNeasy Mini kit (Qiagen, Denmark) according to the manufacturer’s instructions and eluted in 60 µl of nuclease free water ...
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bioRxiv - Cell Biology 2021Quote: ... 3-month-old zebrafish (1 fish/sample) using the RNeasy Mini Kit (Cat. No. 74104; Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
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bioRxiv - Microbiology 2022Quote: ... HSV-1 DNA within cells was isolated from frozen pellets using QIAamp DNAMini Kit (Qiagen: 51304).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was used for cDNA synthesis with the QuantiTect Reverse Transcription kit (Qiagen). Samples were diluted 2.5x after cDNA synthesis and SYBR™ Select Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from approximately 1 million cells per condition using DNA extraction kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... and 200 µM VLCFA mix for 1 d using the RNeasy Plant kit (QIAGEN, Hilden, Germany). For isolation of RNA from the bending site of roots ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: Bulk or flow-sorted THP-1 macrophages were lysed with β-ME-supplemented Buffer RLT (Qiagen). RNA was isolated from the lysate using an RNEasy plus kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells were transfected with 1 μg of pUbg-R252 plasmid using Effectene transfection reagent (QIAGEN) in a 60 mm dish following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of PE buffer and 100 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The silica columns were placed in a collection tube to dry and later in a 1.5 mL DNA lo-bind tube (Eppendorf ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of PBS-AB was added to each tissue with Tungsten carbide 3mm beads (Qiagen). Samples were homogenized for 15 min and then centrifuged at 15,000 g for 10 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from frozen cell pellets of 0.3-1×106 PBMCs (Qiagen, Germantown, MD), and concentrated using an ethanol precipitation protocol with GlycoBlue following manufacturer’s instructions (Thermofisher ...
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bioRxiv - Systems Biology 2023Quote: Small cfRNAs were extracted from ~1 mL of plasma using the miRNeasy Serum/Plasma Kit (Qiagen). 1ul ExiSEQ NGS Spike-in (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... RNAs were converted to cDNA using 1 μg RNA and a QuantiTect Reverse Transcription kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Reverse Transcription was performed on 1 µg of RNA using a QuantiTect Reverse Transcription Kit (Qiagen), and qPCR was performed using a QuantiNova SYBR Green PCR kit (Qiagen) ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 1 mL buffer RLT from the RNeasy mini kit (Qiagen, Germany) with a 1:200 dilution of β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were then treated with DpnI for 1-2 hr and then purified (QiaQuick, Qiagen) before use in library construction.
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was clarified by centrifugation and incubated with 1 mL Ni-NTA resin (Qiagen). The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Genomics 2023Quote: ... with each 20 μL reaction consisting of 1× QuantiFAST reaction mix containing ROX reference dye (Qiagen), 0.66 µM forward and reverse primers ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Microbiology 2023Quote: ... each spot was collected into a 1:2 mix of LBS and RNAprotect Bacteria reagent (Qiagen), incubated at RT for 5 minutes ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a minElute RNA Cleanup Kit (Qiagen). RNA concentrations were measured by Nanodrop and total RNA integrity assayed using an Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total mRNA from each sample was retrotranscribed using QuantiTech reverse Transcription Kit (Qiagen). Real-Time PCR was performed using SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were loaded onto a 1 mL Ni-NTA Superflow column (Qiagen, Valencia, CA, USA) that had been equilibrated with 10 column volumes of wash 1 buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant proteins were purified on Ni-NTA columns (1 ml Ni-NTA Agarose; Qiagen Cat # 30210), washed with 20 ml hexokinase buffer (20 mM HEPES ...