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Citations for Qiagen :
1451 - 1500 of 1600 citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and the amplified DNA bands from the 5’ and 3’ ends were individually excised and purified with QIAquick® Gel Extraction Kit (QIAGEN). Purified PCR products were cloned into pJET1.2/blunt plasmid (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were centrifuged for 5 minutes at 300g and resuspended in 1mL PBS + RNase Inhibitors (Invitrogen AM2694, Qiagen Beverly Inc Y9240L) and fixed as previously described for split-pool ligation-based RNAseq ...
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bioRxiv - Biochemistry 2020Quote: ... embryo pellets were homogenized by cryo-milling after addition of a 2.5 or 5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 15 seconds 3-6 times ...
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bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
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bioRxiv - Genomics 2022Quote: The second lobe of lung or trachea was immersed in 1 mL PBS in a 2 mL Micro Centrifuge Tube (Fisherbrand, 14-666-315) containing one stainless steel bead (5 mm, QIAGEN, 1026563) immediately after dissecting the SARS-CoV-2 infected mouse or hamster ...
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bioRxiv - Genetics 2022Quote: ... 0.08M KOH was added to each well and incubated at room temperature (RT) for 5 min and then washed with EB (Qiagen, 19086). Next ...
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bioRxiv - Genomics 2022Quote: ... The swabs were placed in Red 1.5 mL RINO® screw-cap tubes (NextAdvance) pre-filled with RNase-free zirconium oxide beads and QIAzol Lysis Reagent (Qiagen) was added ...
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bioRxiv - Genetics 2019Quote: ... in 384 well plates in a total volume of 5 μl with 2.5 μl Qiagen Multiplex PCR Plus Kit (Qiagen, Hilden, Germany) and 1 μl Qiagen Q-solution using a Yakima Yellow-labelled primer ...
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bioRxiv - Microbiology 2021Quote: ... 5) 240 µL of the supernatant is subjected to viral RNA extraction using the QIAamp Viral RNA Mini Kit (Qiagen, #52904), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... suspended in 1 mL of (v/v) 50% ACN and homogenized in a bead mill (50 Hz, 5 min; TissueLyser LT, Qiagen, Germany) using two 5 mm tungsten balls ...
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Genomics 2021Quote: ... We checked ASOs penetration in cells by means of the 5′-FAM-labeled control ASO A provided by Qiagen (Figure S5f).
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bioRxiv - Neuroscience 2021Quote: ... 5 ng of each PCR product was pooled into one tube for purification using the QIAquick PCR purification kit (Qiagen #28106) and eluted into 30 µL Nuclease Free Water ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 SCID mT3-2D subcutaneous tumors and the mT3-2D cell line using a DNeasy Blood and Tissue Kit (Qiagen, #69504). Indexed ...
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bioRxiv - Microbiology 2019Quote: ... 500 μL of lysis buffer (TE buffer containing 1% Triton X100) was added into the tube and mixed for 5 min using the Vortex Adapter (13000-V1-24, QIAGEN, Germany). After the centrifugation at 5,000 g for 5 min ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from the colon tissue of mice (n=5) by using the RNeasy mini kit (Cat# 74104, Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD14- CD16- CD20+CD38+ BG505 SOSIP+YU2-gp140+ B cells were sorted into individual wells of a 96-well plates containing 5 μl of a lysis buffer (Qiagen, 1031576) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Microbiology 2020Quote: ... and then with (2) 15 µl Proteinase K [>600 mA/ml] and 5 µl Rnase A [100 mg/ml] (Qiagen, Germany) for 5min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were transferred to a new 0.5ml tube and incubated in 100 μL ChIP elution buffer (10mM Tris-HCl pH8.0, 5mM EDTA, 300mM NaCl, 0.5% SDS) containing 5 µL proteinase K (Qiagen, 20mg/ml stock) at 55°C for 2 h and then at 65°C for 4 h ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Microbiology 2022Quote: ... The cells were then centrifuged at 300g for 5 minutes and lysed using buffer RLT provided in the RNAeasy Plus Micro Kit (Qiagen; 74034). After the lysate was homogenized using the QIAshredder Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 for 72 hours before lysis and RNA extraction using the RNeasy Micro Plus extraction kit (Qiagen, Cat. No. 74034). A further technical replicate was resuspended in RPMI supplemented with 20% FCS ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of plasmid DNA was transfected into each well using Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427). Unless otherwise noted ...
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bioRxiv - Neuroscience 2022Quote: ... or a non-targeting scrambled control (Scr; sequence: 5’-TAACACGTCTATACGCCCA-3; Qiagen, Cat No.: 339137; 0.1 nmol in 2 μL PBS), using a 2 μL Hamilton syringe at a rate of 1 μL/min ...
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bioRxiv - Immunology 2022Quote: ... following the final boost (Week 18 or 23) were sorted into individual wells of a 96-well plate containing 5 μL of lysis buffer (TCL buffer (Qiagen, 1031576) with 1% of 2-β-mercaptoethanol) ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... for 3 h at 37°C under 5% CO2 and total cellular RNA was purified using PureLink™ RNA kit (Life Technology) or RNeasy Mini Kit (Qiagen) and the concentration and RNA Integrity Number (RIN ...
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bioRxiv - Genomics 2023Quote: ... 50 nuclei were sorted into each well of 4 twin.tec™ 96 Well LoBind PCR Plates that had 5 uL of EB buffer (Qiagen, 19086), 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: PCR primers 515/806 with barcoding on the forward primer were used in a 28 cycle PCR (5 cycle used on PCR products) using the HotStar Taq Plus Master Mix Kit (Qiagen, USA) to target the 16S rRNA gene for region V3 and V4 ...
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bioRxiv - Cell Biology 2023Quote: ... Spheroids were transferred into PCR-clean 1.5 mL microtubes with 100 µL of lysis buffer (LB1 from Macherey-Nagel or 700 µL of Buffer RLT (79216, Qiagen). Lysates were snap-frozen and stored at −80 °C ...
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bioRxiv - Microbiology 2022Quote: ... The spooled DNA was washed with approximately 5 mL of 70% ethanol and dried before being suspended in 300µL of EB buffer (QIAGEN, Hilden, Germany). Purified DNA was quantified by Qubit (Thermofisher).
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bioRxiv - Cancer Biology 2023Quote: Ctnnb1 exon 3 skipping was confirmed in several tumour samples by extracting total RNA from 5-20 mg of tissue using the RNeasy Plus Universal minikit (Qiagen, #73404). cDNA was synthesized using the RevertAid protocol (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... 20-40 mg of tissue was placed in a tube containing 600 μl of OptiMEM and a 5 mm stainless steel bead (Qiagen; #69989) and homogenized in the Qiagen TissueLyser II by two dissociation cycles (1,800 oscillations/minute for 2 minutes ...
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bioRxiv - Systems Biology 2024Quote: ... The samples were then cooled for 5 minutes on ice and transferred to a pre-spun 15 mL MaXtract tube (Qiagen 129065) containing an equal volume of chloroform to the acid phenol used ...
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bioRxiv - Developmental Biology 2024Quote: ... 1.5 µg of gDNA was eluted in 15 µl of elution buffer from the EpiTect Fast DNA Bisulfite kit (Qiagen, 59824). A 20 µl PCR reaction was composed of 0.5 µl of bisulfite-converted gDNA input ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from mouse parotid gland tissue (n=5/group) following the manufacturer’s protocol for DNeasy Blood & Tissue Kit (Ref. No. 69504, Qiagen, Hilden, Germany). Mitochondrial DNA (mtDNA ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: Tissue pools were homogenised for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen, Germany). A volume of 0.2× chloroform (Carl Roth ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 mL of the culture was immediately treated with a double volume (10 mL) of RNA protect bacteria reagent (Qiagen, Germany) for 5 minutes at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... trigynum (Sample size: L. tenue thrum=6, pin=4, L. trigynum homostyle=5) using the RNeasy Plant Mini Kit (QIAGEN, Germany). Libraries were sequenced on an Illumina NovaSeq S1 Sequencing System to produce paired-end 150bp read length reads ...
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bioRxiv - Genomics 2023Quote: The bacterial 16S rRNA V4 variable region was amplified for 5 cycles with the HotStarTaq Plus Mastermix Kit (Qiagen, Hilden, Germany). Samples were purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... posterior eye cups (PECs) were separated and collected in 2 mL reinforced tubes (SPEX Sample Prep) containing 5 mM stainless steel beads (Qiagen, LLC).
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bioRxiv - Microbiology 2024Quote: ... and 10 zirconium beads were added to approximately 5 mg of intestinal tissue stored at -80 °C in RNA later tissue reagent (Qiagen, Germany). A Tissue lyser (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: Sample preparation: 20 mg of snap frozen brain tissues were bead homogenized using 5 mm stainless steel bead (QIAGEN Cat#69989) in 0.4 ml of methanol:water (80:20 v/v ...
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bioRxiv - Genomics 2020Quote: ... for 4 hours to inhibit reporter transgene expression (t4) prior to isolation of DNA and RNA using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen# 80004) according to the manufacturers protocol ...
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bioRxiv - Genetics 2019Quote: ... protein altering inframe InDels (n=28) and missense variants (n=557) were uploaded to Ingenuity pathway Analysis (Qiagen, Venlo, The Netherlands). Additionally ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cancer samples were snap-frozen in liquid nitrogen and DNA extracted using the AllPrep DNA/RNA/Protein mini kit (QIAGEN, Germany) as previously reported 18 ...
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bioRxiv - Biochemistry 2020Quote: ... Canonical pathways associated with differentially expressed proteins were identified by Ingenuity Pathway Analysis (IPA, Ingenuity Systems, Qiagen, Redwood, CA, USA; www.ingenuity.com). This program is based on an algorithm that uses curated connectivity information from the literature to determine the network of interactions among the differentially expressed proteins and canonical pathways in which they are involved (Calvano et al. ...