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Citations for Qiagen :
1451 - 1500 of 3031 citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 0.5 mL of each culture was mixed with 1 mL of RNA Bacteria Protect Reagent (Qiagen) immediately after 2 mM ZnCl2 induction (t0 ...
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bioRxiv - Genomics 2021Quote: ... size-selected on a 1% agarose gel and purified using the MinElute PCR Purification Kit (Qiagen). The DNA library was then cloned into the vector using four In-Fusion cloning reactions according to the manufacturer’s instructions (Clontech In-Fusion HD) ...
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bioRxiv - Genomics 2021Quote: ... size-selected on a 1% agarose gel and extracted with the QIAquick Gel Extraction Kit (Qiagen). The eluates were pooled and purified using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: Genomic DNA from 1 g leaf material was isolated using the DNeasy Plant Maxi kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... RNA was extracted from THP-1 and cDNA was synthesized using RT2 First strand kit (Qiagen). Pre-designed RT2 qPCR primer assays (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 ml of PE buffer and 100 μl of EB buffer (MinElute PCR Purification Kit, QIAGEN).
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bioRxiv - Genetics 2021Quote: ... Replicates were lysed in 1 mL of RLT buffer and homogenized using QIAshredder columns (Qiagen, 79654). Total RNA was extracted and eluted in 100 uL RNase-free water using an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL for OD600 ~0.4 for 10% CO2) and stabilized by RNA Protect Bacteria Kit (Qiagen). Next ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA from A549 cells (1 × 106 cells) was extracted using miRNeasy Mini kit (Qiagen, Denmark) according to the manufacturer’s instructions and eluted in 60 µl of nuclease free water ...
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bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
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bioRxiv - Microbiology 2022Quote: ... HSV-1 DNA within cells was isolated from frozen pellets using QIAamp DNAMini Kit (Qiagen: 51304).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was used for cDNA synthesis with the QuantiTect Reverse Transcription kit (Qiagen). Samples were diluted 2.5x after cDNA synthesis and SYBR™ Select Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from approximately 1 million cells per condition using DNA extraction kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... and 200 µM VLCFA mix for 1 d using the RNeasy Plant kit (QIAGEN, Hilden, Germany). For isolation of RNA from the bending site of roots ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells were transfected with 1 μg of pUbg-R252 plasmid using Effectene transfection reagent (QIAGEN) in a 60 mm dish following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of PE buffer and 100 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The silica columns were placed in a collection tube to dry and later in a 1.5 mL DNA lo-bind tube (Eppendorf ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of PBS-AB was added to each tissue with Tungsten carbide 3mm beads (Qiagen). Samples were homogenized for 15 min and then centrifuged at 15,000 g for 10 min at 4°C ...
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bioRxiv - Systems Biology 2023Quote: Small cfRNAs were extracted from ~1 mL of plasma using the miRNeasy Serum/Plasma Kit (Qiagen). 1ul ExiSEQ NGS Spike-in (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... RNAs were converted to cDNA using 1 μg RNA and a QuantiTect Reverse Transcription kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Reverse Transcription was performed on 1 µg of RNA using a QuantiTect Reverse Transcription Kit (Qiagen), and qPCR was performed using a QuantiNova SYBR Green PCR kit (Qiagen) ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 1 mL buffer RLT from the RNeasy mini kit (Qiagen, Germany) with a 1:200 dilution of β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were then treated with DpnI for 1-2 hr and then purified (QiaQuick, Qiagen) before use in library construction.
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from frozen cell pellets of 0.3-1×106 PBMCs (Qiagen, Germantown, MD), and concentrated using an ethanol precipitation protocol with GlycoBlue following manufacturer’s instructions (Thermofisher ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was clarified by centrifugation and incubated with 1 mL Ni-NTA resin (Qiagen). The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Genomics 2023Quote: ... with each 20 μL reaction consisting of 1× QuantiFAST reaction mix containing ROX reference dye (Qiagen), 0.66 µM forward and reverse primers ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a minElute RNA Cleanup Kit (Qiagen). RNA concentrations were measured by Nanodrop and total RNA integrity assayed using an Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... each spot was collected into a 1:2 mix of LBS and RNAprotect Bacteria reagent (Qiagen), incubated at RT for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total mRNA from each sample was retrotranscribed using QuantiTech reverse Transcription Kit (Qiagen). Real-Time PCR was performed using SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were loaded onto a 1 mL Ni-NTA Superflow column (Qiagen, Valencia, CA, USA) that had been equilibrated with 10 column volumes of wash 1 buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant proteins were purified on Ni-NTA columns (1 ml Ni-NTA Agarose; Qiagen Cat # 30210), washed with 20 ml hexokinase buffer (20 mM HEPES ...
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bioRxiv - Systems Biology 2023Quote: ... About 1 OD/mL cells were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, 76506), and the cell pellet was collected by centrifuging at 2500 g for 8 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria (∼1 × 109 cells) were removed from the cultures and suspended in RNAprotect bacteria reagent (Qiagen), pelleted by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from 1 ml of overnight culture (DNeasy Blood and Tissue Kit—Qiagen) pre-treated with 100 μg of lysostaphin (Sigma cat ...
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bioRxiv - Microbiology 2023Quote: ... These were harvested by centrifugation and resuspended in 1 mL of RNA Protect Bacteria Reagent (Qiagen) with 30µL of 20 mg/mL lysozyme ...
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bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
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bioRxiv - Physiology 2023Quote: ... PCR was performed using 1 μl of cDNA and Top Taq DNA polymerase and buffer (Qiagen). The reactions were performed for 30 s at 94 °C ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: Bulk or flow-sorted THP-1 macrophages were lysed with β-ME-supplemented Buffer RLT (Qiagen). RNA was isolated from the lysate using an RNEasy plus kit (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from 1 or 2 bumblebee legs using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 1% v/v sodium dodecyl-sulfate pH 8.0) for protein extraction or in RLT buffer (Qiagen) for RNA extraction ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μg of RNA per experimental sample was processed with the QuantiTect cDNA Synthesis Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Whole genome amplifications were performed on DNA extracts at dilution 10 times through a multiple displacement amplification (MDA) step of 6 to 7 hours using the REPLI-g Midi Kit (QIAGEN) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... we extracted total RNA from CD4+/CD8+/CD3+ T cells (from spleen or bone marrow) of Foxp3-GFP or C57BL/6 mice using RNeasy Micro Kit (Qiagen) and cleaned from excess DNA with DNAse 1 enzyme (Promega) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from blood (n=6 for each housing group) using the RNeasy Protect Animal Blood Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... or trl RNA was was calculated using the 2-ΔΔCt method56 using RNA extracted from 500ul of cells following the 6 day incubation using an RNeasy Plus RNA extraction kit (Qiagen). Gene targets were amplified from cDNA using previously validated primers for clamp ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from frozen mouse liver (20-25 mg) and HepG2 cells (5-6 million) using the AllPrep DNA/RNA Mini Kit (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... was isolated from human PanNET specimens or cells grown on 6-cm or 10-cm plates using RNeasy mini kit (Qiagen) containing gDNA eliminator spin columns ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA was extracted from 2 batches of 50 adult females from each condition (F0 GuyR, F0LD80, F2LD25 and F2LD75, see Fig. 6) using the PureGene kit (Qiagen) following manufacturer’s instructions ...