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Citations for Qiagen :
101 - 150 of 1741 citations for Solute Carrier Family 12 Member 1 SLC12A1 Antibody APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The 12 wells from individual plate rows were pooled and cleaned (QIAquick PCR Purification Kit, QIAGEN), and the 30μl elutions resulting from every prep were combined and vortexed thoroughly ...
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bioRxiv - Microbiology 2024Quote: ... We purified genomic DNA from the 12 lysogens using the DNeasy Blood and Tissue Kit (Qiagen) and determined the genome sequences of Stx2a phages lysogenized in each lysogen by the long PCR-based strategy ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated on day 12-16 by using the DNeasy Blood and Tissue Kit (Qiagen). The FKBP12 site was amplified with the PlatinumTM SuperFi DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... His-tagged proteins were detected with a mouse anti-His HRP-conjugated antibody (1:3000) from Qiagen. MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Neuroscience 2020Quote: ... the 15 mm diameter coverslips were placed in 12 well plate with 600μl of Buffer RLT (Qiagen). These plates were stored at −80 °C until RNA extraction ...
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bioRxiv - Genetics 2020Quote: ... cDNA was amplified with 12 cycles of PCR and purified using the QIAquick PCR Purification Kit (Qiagen). The libraries were size-selected on a 6% polyacrylamide gel ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from Arabidopsis unopened flower buds (younger than stage 12) using RNeasy Mini Kit (Qiagen). cDNA was obtained using Hiscript III 1st Strand cDNA Synthesis Kit + gDNA wiper (Vazyme) ...
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Developmental Biology 2022Quote: ... and the number of transcripts per million (TPM) was determined using the CLC Genomics Workbench 12 (Qiagen, USA). Results were mined for expression of factors implicated in SARS-CoV-2 infection ...
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bioRxiv - Molecular Biology 2023Quote: 12-30 reactions were pooled and purified by PCR column purification using QIAquick PCR purification kit (Qiagen, 28104). DNA concentration was measured by Nanodrop 1000 (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: All datasets were combined (∼250 million reads) assembled de novo with both CLC genomics workbench 12 (Qiagen, 12.0) and Trinity using default settings ...
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bioRxiv - Biochemistry 2023Quote: ... genomic DNA was extracted from 1x107 parasites 12 days after transfection using the DNeasy Blood & Tissue Kit (Qiagen) with non-transfected Cas9 positive and wild type L ...
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bioRxiv - Microbiology 2022Quote: ... α-His antibody (Qiagen) was used at 1:5,000 dilution to detect the presence of rRH5 in Native-PAGE.
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bioRxiv - Genomics 2020Quote: ... The total RNA solution from 12 wells are passed through one column of RNeasy mini kit (cat.74104, QIAGEN) to obtain approximately 100 g of purified total RNA ...
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bioRxiv - Bioengineering 2021Quote: ... The complex (12 mg/ml) was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 12 well plates at a dilution of 1.5×106 and were transfected with the indicated constructs using Effectene (Qiagen) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: 2.0 × 105 HeLa cells were transfected in 12 well plates with control (NTC: all-stars non-targeting control, Qiagen) or DDX39B-targeting (D11 ...
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bioRxiv - Genomics 2022Quote: ... 10 budgerigar samples and 12 zebra finch samples) using the DNeasy® Blood & Tissue Kit (Cat. No. 69581, Qiagen). For PCR ...
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bioRxiv - Cell Biology 2022Quote: siRNA targeting ARRB2 (ARRB2-12, Cat #SI03099103) and a non-targeting control (AllStars, Cat # 1027415) was purchased from Qiagen. A pool of 4 siRNAs targeting human RME-8/DNAJC13 was purchased from Dharmacon/Horizon Discovery (Cat #L-010651-01 ...
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bioRxiv - Microbiology 2022Quote: ... Further sequence analysis of 250-bp paired-end read data was run with CLC Genomics Workbench 12 (Qiagen Bioinformatics). The obtained reads were trimmed with bla and 16S rRNA primer sequences bla_293F ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA precipitation was performed for at least 12 hours and MinElute Spin Columns were used for elution (Qiagen, Germany). Historic samples were processed in a dedicated facility for highly degraded specimens.
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bioRxiv - Immunology 2023Quote: ... The protein complexes were adjusted to 8.6–12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Cancer Biology 2023Quote: ... mixed with 100 μl of serum-free medium and 12 μl of HiPerfect Transfection Reagent (Qiagen, Germantown, MD, USA). Effectiveness of ARSB silencing was confirmed by activity assay ...
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bioRxiv - Neuroscience 2024Quote: ... and 12 hr after addition of uridine by addition of RLT buffer (Qiagen RNeasy Plus Micro Kit, cat. 74034) supplemented with 1 mM DTT ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from 12 bulk soil samples and 153 rhizosphere samples with DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) on 0.25 g of soil ...
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Biochemistry 2022Quote: ... we also probed for the Strep-tag on γTuNA (1:1000 dilution of Strep-tag mouse monoclonal antibody, cat. # 34850, Qiagen). Band intensities were measured in ImageJ and normalized in Prism 7 by the wildtype γTuNA band (positive control) ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
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bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
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bioRxiv - Evolutionary Biology 2023Quote: ... previously coated with the anti-B55δ antibody (1 hour incubation at 4°C, followed by extensive washes) and Nickel beads (Qiagen) coated with 400 μg of 6XHIS-S67thio-S109A-XeARPP19 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells with lysis buffer were pulverized in a 10 mL jar containing a 12 mm grinding ball using a TissueLyser II (QIAGEN) by performing 5 rounds of 3 min at 15 Hz ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA of CDC-EVs (n=12) and MSC-EVs (n=4) was extracted using the miRNeasy Serum/Plasma kit (QIAGEN). Library construction was performed according to the manufacturer’s protocol using the TruSeq small RNA Library Kit (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... Each complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Genomics 2020Quote: ... at which point we combined the resulting solution with 12 mL of PB buffer and followed standard MinElute PCR Purification Kit (Qiagen) protocol ...
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bioRxiv - Genomics 2020Quote: ... total RNA was extracted from cells grown in five different salinity treatments (0, 2, 12, 24, 36 parts per thousand) using the RNeasy Plant Kit (Qiagen), and 15 Illumina libraries were prepared using the Kapa mRNA HyperPrep kit and sequenced on the Illumina HiSeq2000 platform at the Beijing Genomics Institute.
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using the miScript II RT Kit with Hiflex buffer from 12 μL of extracted total RNA according to product manual (Qiagen). Expression of mRNA was quantified using QuantiTect SYBR Green PCR kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... from explants that had been cultured on RIM for 12 hours in the semi-synchronous LR induction system and purified using the RNeasy microkit (QIAGEN). Affymetrix ATH1 microarrays were hybridized with biotinylated cRNA targets prepared from the RNA samples according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The complete dataset of 455,774 sequences with coverage across the genome were aligned in CLCbio Genomics Workbench 12 (QIAGEN Bioinformatics) to the GenBank reference sequence NC_045512.2 ...
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bioRxiv - Systems Biology 2020Quote: ... We incubated these cultures on a shaker at 225 rpm and 32°C for 12-14 hours then pelleted the cultures by centrifugation and isolated plasmid using the EndoFree plasmid maxiprep kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... total RNAs were extracted from 12-day-old seedlings grown during long days using a RNeasy Plant Mini kit (Qiagen). Following RNA purification ...
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bioRxiv - Microbiology 2020Quote: ... then entered an extraction pipeline in which 12 samples were processed in parallel on the QIAvac 24 Plus Manifold (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... The input and immunoprecipiated DNA were reverse cross-linked at 60 degrees for 12 hours and then purified using a QIAquick PCR Purification kit (QIAGEN). The input DNA was diluted 1:100 ...
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bioRxiv - Genetics 2022Quote: ... and larvae (12 CRISPR-treated, 20 water-injected and 13 uninjected) selected for sequencing by using DNeasy Blood & Tissue Kit (QIAGEN) Purity and concentration of DNA samples were measured with a NanoDrop spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: DNA was isolated from PBMC and lymph nodes cells collected 12 weeks post-challenge using the QIAamp micro kit (Qiagen) following the manufacturer’s instructions and eluted in 50 μL of water ...
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bioRxiv - Microbiology 2021Quote: ... The protein complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Cell Biology 2021Quote: ... 10∼12 ml cell culture medium was subjected to extracellular RNA isolation using the exoRNeasy Maxi kit (Qiagen, Cat# 77164) with minor modifications ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...