Labshake search
Citations for Qiagen :
1 - 50 of 1741 citations for Solute Carrier Family 12 Member 1 SLC12A1 Antibody APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: DNA was extracted from all family members from whole blood using Puregene chemistry (Qiagen). Exome capture was undertaken in both affected individuals using the SureSelect 50 Mb All Exon Kit v3 (Agilent ...
-
bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from various tissue samples collected from patients and their family members with a Gentra DNA extraction kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2020Quote: ... 1 µl 1 mg/ml Carrier RNA (QIAGEN), 1 µl 10% SDS ...
-
bioRxiv - Genomics 2023Quote: ... Genomic DNA for Family 1100 and Family 2100 was extracted with the MagAttract HMW DNA Kit (Qiagen) from whole blood samples of individuals enrolled in a human subjects research protocol approved by the New York University Grossman School of Medicine Institutional Review Board.
-
bioRxiv - Genomics 2023Quote: ... 1 µl carrier RNA (1µg, from Qiagen, cat. no. 59824), 4 µl of Dr.GenTLE precipitation carrier (Takara ...
-
bioRxiv - Genomics 2024Quote: ... The 94-member library was purified using the Midi Plus kit (Qiagen). Group 2 amplicons were then cloned into the 94-member library using 8x one pot Golden Gate cloning with Esp3I as described in plasmid generation methods ...
-
bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
-
bioRxiv - Microbiology 2023Quote: ... Dilutions of 104-101 plasmid copies contained 1 ng carrier RNA (QIAGEN). QPCR was performed in a LightCycler 480 Instrument II (Roche ...
-
bioRxiv - Developmental Biology 2021Quote: ... with carrier RNA (Qiagen, Cat 59824) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... and carrier RNA (100μl of 1μg/μl stock; Qiagen). The swab is snapped carefully to avoid disturbing the buffer and the cap is place back onto tube containing the buffer and swab and tightened ...
-
bioRxiv - Bioengineering 2021Quote: ... GBlocks were serially diluted with 100ng/μL carrier RNA (Qiagen, 1017647) in 1× IDTE buffer with 0.1% TWEEN 20 (Sigma Aldrich).
-
bioRxiv - Genomics 2022Quote: ... The RNA was diluted in carrier tRNA (Qiagen, Cat. No. 1068337) enriched with molecular water 1:5 for target genes or 1:500 for 28S rRNA ...
-
bioRxiv - Neuroscience 2021Quote: ... qPCR for individual miR-17∼92 members was performed using miRCURY LNA probes and miRCURY LNA SYBR Green PCR Kit (Qiagen).
-
bioRxiv - Biophysics 2020Quote: Genomic DNA from Pitohui uropygialis meridionalis (Family Oriolidae) blood and tissue was extracted using DNeasy kits (Qiagen) to create whole genome sequence libraries for the poisonous Pitohui birds ...
-
bioRxiv - Developmental Biology 2022Quote: ... Gene expression of different fos family genes in zebrafish was analyzed using Quantitect™ Primer Assays (Qiagen) for each individual gene – fosaa ...
-
bioRxiv - Plant Biology 2024Quote: ... eight discs from each family were homogenized with beads in a TissueLyser® disruptor (Qiagen, Hilden, Germany) to perform DNA extractions using the CTAB protocol (Doyle ...
-
bioRxiv - Neuroscience 2022Quote: SARS-CoV-2 RNA was harvested from the cell medium at various time points post infection and stored in viral lysis buffer with RNA supplements (50 μl of sample medium in 560 μl of AVL buffer supplemented with 1% carrier RNA) (Qiagen). RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen) ...
-
bioRxiv - Microbiology 2024Quote: We harvested SARS-CoV-2 RNA by collecting 25 μL of cell culture medium at various time points post infection and stored in viral lysis buffer with RNA supplements (AVL buffer supplemented with 1% carrier RNA; Qiagen). We extracted RNA using a QIAamp viral RNA mini kit (Qiagen) ...
-
bioRxiv - Microbiology 2023Quote: ... we used low-binding plastics and added 1 µl of 0.1 ng/µL tRNA carrier (i.e. spike water, Qiagen, Cat. No. 1068337) in each tube with stock eluted DNA ...
-
bioRxiv - Microbiology 2021Quote: ... which included the use of Qiagen Protease and carrier RNA (Qiagen Sciences, Maryland, MD). Samples were eluted in 75 μL of Buffer AVE (Qiagen Sciences ...
-
bioRxiv - Genetics 2022Quote: ... The total samples or supernatants were digested by adding 10ng carrier RNA (Qiagen, 1068337), 1μL 20% SDS and 2μL Proteinase K (Roche) ...
-
bioRxiv - Genomics 2020Quote: A 560 μL volume of lysis buffer containing guanidinium and carrier-RNA (QIAGEN, Hilden, Germany) was added to 200 μL supernatant from cell cultures ...
-
bioRxiv - Developmental Biology 2021Quote: ... 4 ng/μL RNA carrier as described by the supplier of the RNeasy® Micro kit (Qiagen, Hilden, Germany).
-
bioRxiv - Systems Biology 2022Quote: ... 12 μl of 1 M Tris-HCl (pH 6.5) and 1 μl RNAse A (Qiagen cat # 19101) were added to the sample and incubated for 30 min at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: ... 100 μl of blood from K2-EDTA collection tubes was collected prior to centrifugation and was added to 600 μl of AVL viral lysis buffer with 6 μL carrier RNA (Qiagen) for RNA extraction ...
-
bioRxiv - Genomics 2023Quote: ... After ligation nuclei were pelleted and resuspended in cold PBS with DAPI to a final concentration of 300nM and GFP+ cells were FAC-sorted into a 96 well plate with 2ul lysis buffer (20mM Tris pH 8, 20mM NaCl, 0.15% Triton X-100, 25mM DTT, 1mM EDTA, 500nM Carrier ssDNA, and 15ug/mL Qiagen Protease) and lysed for 1 hour at 50°C and inactivated 15 minutes at 70°C ...
-
bioRxiv - Genetics 2024Quote: ... DNA samples (70–80 oocytes and 7–10 blastocysts per sample) were subjected to bisulfite conversion through 2 µg carrier RNA (QIAGEN). Nested PCR was performed using EpiTaq HS with the primers listed in Supplementary Table 4 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were lysed with 4ul of Dip-C Lysis buffer (25mM DTT, 20mM Tris pH8, 0.15% Triton X-100, 500nM Carrier ssDNA, 20mM NaCl, 1mM EDTA, 15ug/ml Protease, Qiagen, 19155) for 1hr at 50°C followed 70°C for 15 min (heat inactivation) ...
-
bioRxiv - Neuroscience 2023Quote: ... samples obtained by FACS together with 100 ng carrier RNA (PolyA- and PolyA+ mixed in 98:2 ratio) were lysed by Buffer RLT Plus (Qiagen, USA). Then we used PolyA mRNA Magnetic Isolation Module (NEBNext ...
-
bioRxiv - Neuroscience 2023Quote: ... 12 µL of Enhancer (Qiagen), 16 µg of plasmid and 15 µL of Effectene (Qiagen) ...
-
bioRxiv - Microbiology 2020Quote: ... adding 560 μL viral lysis buffer (AVL buffer including carrier RNA; AVL buffer) from the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) for 10 min at room temperature according the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from the nasal swab using the QIAmp viral RNA mini kit without the addition of carrier RNA (Qiagen, Manchester, UK). cDNA was synthesised from RNA using a random hexamer primer mix and cDNA Synthesis System (Roche ...
-
bioRxiv - Microbiology 2024Quote: ... Protein purity and stability was confirmed via SDS-PAGE using 12% polyacrylamide gels and Western blotting using Penta-His antibody (QIAGEN) (Fig ...
-
bioRxiv - Genomics 2022Quote: ... and CLC Genomics Workbench 12 (Qiagen) all program arguments available in GitHub repository ...
-
bioRxiv - Microbiology 2020Quote: ... CLC Genomics Workbench version 12 (Qiagen) was used to de novo assemble WT ATCC 700057 ...
-
bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... A supplement of 6 µL carrier RNA (prepared by mixing 310 µl of Buffer EB from DNeasy PowerWater Kit with 310 µg lyophilized carrier RNA (QIAGEN, Cat. no. 1068337)) was mixed with 600 µL of recovered supernatant ...
-
bioRxiv - Microbiology 2020Quote: ... and incubated with primary antibody (Penta·His Antibody, QIAGEN®, 1:2000 dilution) in 1% bovine serum albumin in PBST for 2h at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... APC-negative cells were single-cell sorted by FACS into TCL buffer (QIAGEN) containing 1% 2-mercaptoethanol ...
-
bioRxiv - Microbiology 2022Quote: ... After incubation with the primary antibody from QIAGEN (Penta-His Antibody, 1:1000 dilution) at RT for 1 h under shaking ...
-
bioRxiv - Biochemistry 2022Quote: ... Penta-His Antibody (Qiagen, 34660, IB: 1:1000) and Y188 to APP C-terminus (Abcam ...
-
bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
-
bioRxiv - Cell Biology 2022Quote: Ingenuity Pathways Analysis (IPA) (Qiagen, version 01-12) was used to examine the various comparisons ...
-
bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
-
bioRxiv - Cell Biology 2024Quote: For optimisation of pericyte growth conditions RNA was collected from pericytes seeded at 1×105 in a 12-well plate using the RNeasy Mini Kit (74104, Qiagen) after 24 h in the appropriate GA medium ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse antibodies against the His-tag (1:1000, Qiagen), rabbit antibodies against the C-terminal region of the collagen VI α1 chain (1:1000 ...
-
bioRxiv - Microbiology 2021Quote: ... Data was analyzed with CLC Genomics Workbench 12 (QIAGEN) by mapping the human reads onto the hg19 reference genome scaffold (GCA_000001405.28) ...
-
bioRxiv - Genomics 2022Quote: ... 12 µl proteinase K (14-22 mg/mL, Qiagen), and 12 µl β– mercaptoethanol) ...
-
bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
-
bioRxiv - Neuroscience 2022Quote: ... with the final elution in 12 μl of EB solution (Qiagen). For tagmentation the Nextera DNA Sample Preparation kit (FC-131-1096 ...