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Citations for Qiagen :
101 - 138 of 138 citations for Smooth Muscle Actin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Frozen muscle was pulverized in liquid nitrogen and genomic DNA was extracted using a Genomic Tip 100/G kit (Qiagen, USA). DNA quantity was measured using a NanoDrop ND-8000 spectrophotometer and a Qubit dsDNA BR Assay kit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA was extracted from leg and/or thoracic muscle tissue using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... or a small portion of the tail area (skin, muscle and bone) was by QIAzol Lysis Reagent according to the Qiazol Protocol (Qiagen, Germany); from livers and intestines of the adult fish ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from the thoracic muscle tissue of each individual using either the QIAamp 96 DNA Blood Kit or the DNeasy Blood & Tissue Kits (Qiagen, LLC). Libraries were prepared for low-coverage whole genome sequencing (2×150 bp reads ...
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bioRxiv - Genomics 2023Quote: ... Total genomic DNA was extracted from thorax muscle tissue and purified using the Qiagen Blood & Culture DNA Midi Kit (Qiagen, Germany). Libraries (n = 39 ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from freshly sorted 30,000 PDGFRα+ cells from LA/BC muscles using the RNeasy Plus Micro Kit (Qiagen, Cat# 74034) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... muscle samples were used to obtain high molecular weight gDNA using the QIAGEN MagAttract HMW DNA kit (QIAGEN, Germantown, MD, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted genomic DNA from muscle tissue of the AK hare sample following the Qiagen DNeasy Blood and Tissue kit protocol (Qiagen, Valencia, CA). For the pooled MT whole genome sequencing ...
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bioRxiv - Genomics 2020Quote: ... and OA (from Wuxi Freshwater Fisheries Center in China) were extracted from muscle tissues using a Blood & Cell Culture DNA Midi Kit (Q13343, Qiagen, CA, USA). We also obtained genomic DNAs from ONJp with a YY genotype ...
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bioRxiv - Evolutionary Biology 2022Quote: We extracted DNA either from the adductor muscles or the foot using the DNeasy Blood and Tissue Kit (QIAGEN GmbH, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was isolated from soleus muscle (10 µg) and apex of ventricle (20 µg) using RNeasy fibrous tissue mini kit (QIAGEN, Hilden, Germany) following manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... total DNA was extracted from approximately 15 mg of muscle tissue and 300 mg of bone marrow using the QiAmp DNA mini kit (Qiagen, Valencia, CA). In subsequent experiments ...
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bioRxiv - Genomics 2022Quote: ... we extracted total RNA from eight snap-frozen tissues kept at -80°C (brain, breast muscle, heart, kidney, liver, lung, spleen, and testis) using the RNeasy Mini Kit (Qiagen; Hombrechtikon, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The peptide-coupled proteins were separated from uncoupled proteins using Ni-NTA Agarose (Qiagen).
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Cancer Biology 2023Quote: ... Exceptions to this were made in the case of adipose tissue and skeletal muscle for which we utilized a RNeasy Lipid Tissue extraction Kit and QIAzol Lysis Reagent (Qiagen, Inc., Germantown, MD), respectively ...
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bioRxiv - Physiology 2023Quote: ... genomic DNA was extracted from CD31+ cells isolated from skeletal muscle and liver from AHRfl/fl and AHRecKO mice using Qiagen DNeasy kit (Qiagen, Cat. No. 69504) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA was extracted from muscle tissue or fin clips using a modified salting-out method [44] or a Qiagen DNeasy kit (Qiagen Inc., Valencia, CA, USA). Genomic DNA was checked for quality using a spectrophotometer (NanoDrop ...
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bioRxiv - Genomics 2023Quote: ... DNA for library preparations and sequencing was extracted using a standard phenol-chloroform extraction from liver and muscle samples which were collected at slaughter and stored in stored in RNAlater® Solution (Ambion/QIAGEN, Valencia, CA, USA).
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bioRxiv - Cell Biology 2022Quote: siRNA duplex for IPO9 was obtained from Ambion (id# S31299) and siRNA for the nuclear actin exporter XPO6 was obtained from Qiagen (id# SI00764099). All siRNA were transfected or co-transfected with emerin plasmids at 25 nM using X-tremeGENE HP (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... The sample was subsequently incubated with 80 µL strept-actin resin (Strep-Tactin Superflow Plus; QIAGEN) at room-temperature for 2 h with constant agitation on a horizontal roller mixer ...
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bioRxiv - Cancer Biology 2023Quote: ... were transfected with 10 nM Malat1 ENE-targeting (ASO) or control (Con) steric blocking ASOs (Exiqon, Qiagen) using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: The constructs were transfected in S2R+ cells stably expressing Actin-GAL4 using Effectene (Effectene Qiagen Kit [301425]) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... IL-6 and β-actin cDNA were determined by QuantiFast SYBR Green PCR Master Mix (Qiagen, Hilden, Germany) at the conditions ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled ORC were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer B with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled Cdt1 were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer D with 10 mM imidazole for 1.5 hr with rotation at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.2 μM gene specific primers pairs (hVDAC1, hVDAC2, hVDAC3, β-actin (Table 1) and 12.5 μl of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Three independent experiments of quantitative real-time were performed in triplicate for each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.2 uM gene specific primers pairs (hVDAC1, hVDAC2, hVDAC3, ß-actin) and 12.5 ul of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Three independent experiments of quantitative real-time were performed in triplicate for each sample ...
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bioRxiv - Genomics 2020Quote: ... Obtained fully tryptic peptides were mapped to the secalin and nsLTP sequences in CLC Genomics Workbench v12 (Qiagen, Aarhus, Denmark) using 100% sequence identity to confirm the expression at individual protein levels.
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bioRxiv - Molecular Biology 2020Quote: ... level relative to nuclear gene (β actin) by real-time PCR in total DNA isolated from INS1 cells by QIAamp DNA minikit (Qiagen). Rat primers were mt-ND6 ...
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked for 1 hour at room temperature either with a commercial blocking buffer (QIAGEN, anti RGS HIS6 HRP conjugate kit) or with 5% milk in 0.05% tween TBS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA was extracted from the control and the peptide-treated frozen cells using the Blood & Cell Culture DNA Midi Kit according to manufacturer’s instructions (Qiagen, ref. no. 13343). A first PCR was performed to amplify the lentiCRISPR sgRNA region using the following primers:
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI ...