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Citations for Qiagen :
51 - 100 of 138 citations for Smooth Muscle Actin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... GAS and SOL muscles from 6 mice per group were pulverized and lysed in RLT buffer (Qiagen) and treated with proteinase K (Qiagen ...
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bioRxiv - Pathology 2020Quote: ... Total RNA was extracted from muscle samples using a RNeasy Fibrous Tissue Mini Kit (Qiagen, CA, USA). Total RNA was used as a template with a QuantiTect® Reverse Transcription Kit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... All muscle were homogenized 2 x 30 sec at 30 Hz using a Tissuelyser II (Qiagen, USA) in ice-cold homogenization buffer (10% (v/v ...
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bioRxiv - Genomics 2022Quote: DNA was extracted from 13 mg of muscle tissue using a DNeasy Blood and Tissue kit (Qiagen), quantified using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... high molecular weight genomic DNA was isolated from thoracic muscle tissue using DNeasy Blood & Tissue kits (Qiagen).
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from soleus and EDL muscle using an miRNeasy mini kit (Qiagen, Venlo, Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... fat and skeletal muscle of mice receiving either IgG or X203 using RNeasy Mini Kit (74104, Qiagen) and quantified using Qubit™ RNA Broad Range Assay Kit (Q10210 ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from skeletal muscles using the Qiagen miRNeasy Mini kit (cat# 217004, Qiagen, GmbH) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from liver and muscle using DNeasy Blood and Tissue kits (Qiagen, Chadstone, VIC, Australia) and following the manufacturer protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Genomic DNA was isolated from muscle tissue of the fixed specimens using the DNeasy Blood & Tissue Kit (Qiagen), and the DNA concentration was determined with a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher).
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bioRxiv - Molecular Biology 2021Quote: 50-100 mg of frozen gastrocnemius muscle was minced and homogenized using a Tissue Lyser II homogenizer (Qiagen). The muscle homogenate was mixed with 5 µl of isotope-labelled LM internal standards (IS ...
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bioRxiv - Zoology 2019Quote: We extracted genomic DNA for each specimen from muscle tissues using Gentra Puregene Tissus Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from snap-frozen quadriceps muscles (n=4/genotype/sex) using RNeasy mini kit (Qiagen). The quality of total RNA was validated by Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... an alignment of SPLMh with all 52 protein sequences detected via BLASTp search, was created using MUSCLE v3.8.425 (Edgar, 2004) implementation of CLC Main Workbench 20.0.1 (QIAGEN, Aarhus, Denmark). The alignment was visualised using the CLC Main Workbench.
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from muscle or myonuclei using QIAamp DNA Micro and Mini Kit (Qiagen, Hilden, Germany). Myonuclei were isolated as previously described (Habib et al. ...
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bioRxiv - Evolutionary Biology 2021Quote: High quality genomic DNA was isolated from adult beetle thoracic muscle tissue using DNeasy Blood & Tissue kits (Qiagen) and then submitted to the University of Wisconsin-Madison Biotechnology Center ...
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bioRxiv - Developmental Biology 2022Quote: Total RNAs were extracted from chick limbs or muscle cell cultures using the RNeasy mini kit from Qiagen. 500ng to 1µg RNAs were reverse-transcribed using the High Capacity Retrotranscription kit (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNAs from fat pads and gastrocnemius muscle were isolated using a RNeasy Mini kit (74106, Qiagen, Germany) and RNA Fibrous Tissue Mini kit (74704 ...
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bioRxiv - Genomics 2023Quote: DNA was extracted from muscle samples of the individual animals using the Gentra Puregene Tissue Kit (QIAGEN, Australia), following the manufacturer’s protocols with the modifications described below ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from either fin clips or muscles following protocols of DNeasy Blood & Tissue Kit (QIAGEN). After we prepared Paired-end libraries following protocols of TruSeq DNA PCR-Free LT sample Prep Kit ...
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bioRxiv - Cell Biology 2022Quote: ... The metaphysis and diaphysis were also cleaned of the muscle and connective tissue and homogenized in QIAzol reagent (Qiagen). The human bone biopsy samples used in this study were as described in previous publications (Farr et al. ...
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bioRxiv - Genomics 2021Quote: ... high-molecular-mass (100–200 kbp) genomic DNA from muscle biopsy was isolated using a MagAttract HMW kit according to the manufacturer’s instructions (QIAgen). DNA quantity and quality were evaluated on genomic DNA screentape using a 4200 Tapestation (Agilent ...
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bioRxiv - Bioengineering 2020Quote: Tissue-engineered skeletal muscle was thawed and suspended in 500μL TRIzol™ (Fisher) before being homogenized using TissueLyserII (Qiagen) for ≥2 minutes until tissue degradation was complete ...
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bioRxiv - Genomics 2021Quote: Total DNA was extracted from muscle tissue of one male fish using a QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Sixty 10 μm cryosections of quadriceps muscle snap frozen in liquid nitrogen were collected in QIAzole (Qiagen Cat#79306). Four male and four female wildtype and Col6a2-/- mice were included at two different age groups (5 weeks and 25 weeks old) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from the whole TA muscles of control or CKD mice using Qiazol (Qiagen, Germantown, MD) and then precipitated in isopropanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted from frozen tissue (either muscle, liver, or a fin clip) using a MagAttract HMW DNA Kit (Qiagen), following the manufacturer’s protocol for 25 mg tissue samples ...
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bioRxiv - Genomics 2020Quote: ... DNA extraction from the liver or fin and pectoral fin muscles of the fish from which sperm was collected were performed using DNeasy Blood & Tissue Kit (QIAGEN).
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bioRxiv - Biochemistry 2020Quote: ... Attached figures were prepared in PYMOL [62] the multiple sequence alignment was performed using MUSCLE [63] and the LOGO representation was obtained in CLC Main Workbench (CLC Bio, Qiagen).
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bioRxiv - Cell Biology 2020Quote: DNA and proteins were extracted from approximately 30 of quadriceps muscles using AllPrep DNA/RNA/Protein Mini kit (#80004, Qiagen). DNA concentrations were determined by Thermo Scientific NanoDrop 2000c spectrophotometer ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA from TA muscle was extracted and purified using the RNeasy Fibrous Tissue Mini Kit (74704, QIAGEN, Germantown, MD). RNA samples were transcribed to cDNA by Superscript III First Strand Synthesis Supermix (18080-400 ...
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bioRxiv - Cell Biology 2021Quote: ... Muscle samples were next lysed in ice-cold radioimmunoprecipitation assay (RIPA) buffer (400 μL/muscle) with protease and phosphatase inhibitor cocktail (Cell Signalling) using TissueLyser II (QIAGEN). Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Cell Biology 2021Quote: ... primary muscle progenitor cells were transfected with 100nM of miR-24-3p mimic (Syn-mmu-miR-24-3p, Qiagen, MSY0000219), 100nM of miR-24-3p inhibitor (Anti-mmu-miR-24-3p ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated from selected individuals from both liver and muscle using DNeasy blood and tissue kits (Qiagen, Hilden, Germany). Primers were designed to specifically amplify the regions around the CRISPR gRNA target sites (Table 1) ...
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bioRxiv - Pathology 2021Quote: Total RNA from either snap-frozen muscles or cultured cells was isolated using an RNeasy Micro Kit (Qiagen, Hilden, Germany). The mRNA was reverse-transcribed into cDNA with PrimeScript RT Master Mix (TaKaRa ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted from the muscles of the female adult golden pompano using the Blood & Cell Culture DNA Mini Kit (Qiagen). DNA concentrations and quality were measured using a NanoDrop 2000 (Thermo ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from muscle or whole animal using phenol-chloroform-isoamyl alcohol extraction or MagAttract HMW DNA Kit (Qiagen), and further purified using NucleoBond columns (Machery Nagel) ...
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bioRxiv - Bioengineering 2023Quote: Total DNA was isolated from animal tissues (cortex, cerebellum, liver, heart, muscle) using the Qiagen DNeasy Blood & Tissue Kits (QIAGEN). The quantification of vector transgene expression was calculated by qRT-PCR relative to the ZsGreen transgene using the following primers ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from flash frozen 8 μm thick sections of tibialis anterior muscles embedded in OCT using the RNeasy Mini Kit (Qiagen) and evaluated using the RNA high sensitivity kit (Agilent RNA 6000 Pico Kit ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from muscle tissue using the DNeasy Blood and Tissue Kit following the manufactures’ protocol (QIAGEN, Canada).
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bioRxiv - Molecular Biology 2023Quote: Total RNA from ∼10–60mg of muscle tissue was extracted using a Qiagen miRNeasy Mini kit (217004, Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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Phenogenomic resources immortalized in a panel of wild-derived strains of five species of house micebioRxiv - Evolutionary Biology 2023Quote: Genomic DNA was isolated from frozen (−80 °C) or alcohol-preserved muscle or spleen tissues using DNeasy Blood & Tissue Kits (Qiagen). High-quality DNA aliquots were next-generation sequenced at the Edinburgh Genomics facility using HiSeq X technology ...
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bioRxiv - Immunology 2021Quote: ... and an undisclosed peptide pool inducing CD8+ T lymphocyte stimulation (Qiagen, 2017); rmsHBHA which tubes contain recombinant M ...
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bioRxiv - Genetics 2020Quote: ... with beta-actin as a reference gene (QuantiFast SYBR Green PCR Kit; Qiagen) on a Roche LightCycler 480 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total DNA was extracted from muscles of each of the 20 individuals using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). Field collections were conducted with permission from the Ministry of Research ...
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bioRxiv - Genomics 2021Quote: ... high-molecular-weight genomic DNA (HMW-gDNA) was extracted from homogenised liver and muscle tissues (<25mg) using QIAamp DNA Mini Kit (Qiagen 51304) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 10 to 20 mg of frozen muscle was homogenised two times for two minutes at a speed of 30 Hz with a TyssueLyser instrument (Qiagen, Canada) in an ice-cold lysis buffer (1:20 w/v ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We extracted genomic DNA from legs and/or thoracic muscle tissue using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). Polymerase chain reactions (PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... while RNA from murine muscles (as described in the animal study below) was extracted using RNeasy Fibrous Tissue kit (QIAgen, UK), following the manufacturer’s instructions ...