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Citations for Qiagen :
101 - 150 of 250 citations for QT Nickel Plating Process Agent For Removing Copper Impurity since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Soluble ACE 2 was purified using Nickel -NTA agarose beads (Qiagen Cat.No-1018244), eluted with 200mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... and the soluble fractions were purified using nickel affinity chromatography (Qiagen, Manchester, UK) and eluted with lysis buffer containing 300 mM imidazole ...
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bioRxiv - Cancer Biology 2022Quote: ... and the supernatants were purified using a nickel-NTA affinity column (Qiagen 30210). Centrifugal filters with molecular 10 kDa molecular weight cutoffs (Millipore UFC801024 ...
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bioRxiv - Cancer Biology 2023Quote: ... all proteins were purified using Ni-NTA (nickel-nitrilotriacetic acid) resin from QIAGEN. After the incubation of Expi293 media supernatant with the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Nickel sepharose according to the manufacturer’s instructions (QIAGEN). The purified recombinant protein was dialyzed overnight against dialysis buffer (20 mM Tris-HCl ...
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bioRxiv - Bioengineering 2024Quote: ... AbLecs were purified by manual gravity column using nickel-NTA agarose resin (Qiagen). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... Affinity purification was performed using nickel nitriloacetic acid (Ni-NTA) affinity column (Qiagen) attached to an AKTA™ start system (Cytiva) ...
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bioRxiv - Microbiology 2020Quote: ... The RNA extraction process was referred to the instructions of the kit (RNeasy Mini kit, QIAGEN, Germany). The extracted RNA was detected by ultra-fine ultraviolet spectrophotometer (NanoDrop ...
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bioRxiv - Developmental Biology 2020Quote: ... 565 E.coli colonies per guide ratio (>500x coverage) was used to process the 4 Maxi-Prep (Qiagen) reactions ...
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bioRxiv - Neuroscience 2022Quote: ... QRT-PCR was performed according to a two-step process using the Quantitect Reverse Transcription Kit (Qiagen) for cDNA synthesis ...
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bioRxiv - Microbiology 2024Quote: ... The extraction process was executed according to the manufacturer’s protocol of the DNeasy PowerSoil Kit (Qiagen, Germany). The extracted DNA was quantified using a NanoDrop spectrophotometer (if applicable ...
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bioRxiv - Developmental Biology 2021Quote: ... Free genomic DNA released by the transposition process was purified using the MinElute PCR Purification kit (Qiagen 28004) and then indexed using forward (i7 ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was loaded on a nickel nitrilotriacetic acid (Ni-NTA) resin (Qiagen GmbH) column ...
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bioRxiv - Cell Biology 2021Quote: ... We purified the 6xHIS fusion protein on nickel-nitrilotriacetic acid agarose (Qiagen, Valencia, CA) according to the manufacturer’s protocols ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: ... and nickel-nitrilotriacetic acid (Ni-NTA) agarose resin was purchased from Qiagen (Hilden, Germany). PCR reagents ...
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bioRxiv - Biophysics 2020Quote: ... The enzyme was purified through a nickel-nitrilotriacetic acid column (Ni-NTA superflow, Qiagen), which was followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Cell Biology 2020Quote: ... were purified by two rounds of nickel affinity chromatography using Ni-NTA agarose (QIAGEN), with overnight cleavage by N-terminally His-tagged TEV protease (expressed in our laboratory ...
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bioRxiv - Cell Biology 2020Quote: ... Soluble material was then were added to NTA nickel resin (400 µL slurry, Qiagen), which was pre-charged (overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM 2-mercaptoethanol) and the proteins eluted from nickel-NTA agarose beads (Qiagen; using buffer containing 25 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant HisCheY or HisCheY* and bound proteins were purified using nickel agarose columns (Qiagen) under native conditions per manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Molecular Biology 2022Quote: ... added to 80 μl washed Ni-NTA agarose nickel beads slurry (Qiagen, Cat# 30230), and transferred to Pierce™ Spin Columns – Snap Cap (Thermo Scientific™ ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... S1 RBD was purified from the culture supernatant by nickel–nitrilotriacetic acid agarose (Qiagen), and purity was confirmed to by >95% as judged by coomassie stained SDS-PAGE ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were incubated with Nickel-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) at 4°C with slight shaking for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... coli Rosetta cells was carried out using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) as described in Barja and Rodríguez-Concepción (2020) ...
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bioRxiv - Molecular Biology 2021Quote: ... we added 70 µl of Ni-NTA (Nickel Nitrilo-triacetic Acid) agarose beads (Qiagen), and incubated the mixture overnight at the orbital rotator at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Nickel affinity chromatography was performed on the diafiltered supernatant with Ni-NTA agarose (Qiagen), eluting fractions with increasing concentrations of imidazole in TIM Buffer 1 ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 ml of culture supernatant was incubated with nickel-nitrilotriacetic acid-agarose beads (QIAGEN). The beads were washed ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was applied to a 5 ml nickel-nitrilotriacetic acid column (Qiagen, Valencia, CA) that had been equilibrated with buffer A at a rate of 1 ml/min ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified from filtered supernatants using nickel nitriloacetic acid (Ni-NTA) agarose (QIAGEN) via gravity flow ...
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bioRxiv - Molecular Biology 2023Quote: ... After purification of AtH2B.9 using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAGEN), the His6-tag portion was removed by TEV protease (30 µg/mg of His6-H2B.9) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The clarified lysate was incubated with nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (Qiagen) for 1 hour with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was loaded onto a nickel-nitriloacetic acid (Ni2+-NTA) column (Qiagen, Hilden, Germany), washed with 500ml buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was incubated with 3 ml nickel- nitrilotriacetic acid Ni-NTA beads (Qiagen) preequilibrated with the same buffer overnight ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were subjected to pull down using nickel-nitrilotriacetic acid (Ni-NTA) beads (Qiagen) overnight at 4 °C on a rotation platform ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was then mixed with 5 mL of nickel-NTA agarose resin (Qiagen) equilibrated with the lysis buffer ...
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bioRxiv - Neuroscience 2024Quote: ... for GST-tagged CMK-1 variants or nickel-nitrilotriacetic acid beads (Ni-NTA, Qiagen) in the presence of 15 mM imidazole for TAX-6-His6 protein binding ...
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bioRxiv - Pathology 2023Quote: ... After DNase I digestion and removing the ribosomal RNAs (rRNAs) from total RNA using the QIAseq FastSelect-rRNA Plant Kit probe (QIAGEN), several Adenine bases were tailed at the 3’ end of the remaining RNAs according to Liefting et al (47) ...
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bioRxiv - Bioengineering 2024Quote: RNA was extracted from the cells after dynamic culture by removing the medium from the channels and adding RLT buffer (RNeasy Kit, Qiagen) for cell lysis and consequent RNA isolation ...
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bioRxiv - Biochemistry 2020Quote: ... Amylose elution pool was bound to Nickel NTA (NiNTA) affinity beads (Qiagen, Germantown MD, USA), washed with NiWB (500 mM Sodium Chloride ...
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bioRxiv - Synthetic Biology 2020Quote: ... The soluble fraction was passed through 3ml of nickel nitrilotriacetic acid agarose (Ni-NTA) (Qiagen), washed with 20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a nickel affinity column (Ni-NTA superflow columns from Qiagen) that was previously equilibrated with start buffer ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged AC was secreted into the medium and purified by nickel affinity chromatography (Qiagen) with elution in 100 mM citrate pH 4.0.
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bioRxiv - Biochemistry 2022Quote: ... Sso7dmut-sortase cleavage site-IN was purified with nickel affinity chromatography (Ni-NTA Superflow, Qiagen). The hexahistidine tag and Sso7dmut were cleaved from HIV-1 IN by addition of his-tagged sortase and GGGC peptide (GenScript) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Recombinant Fab proteins were purified from the culture supernatant by nickel-nitrilotriacetic acid agarose (Qiagen). Recombinant mAbs were affinity purified by MabSelect resin (Cytiva ...
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bioRxiv - Physiology 2024Quote: ... C3 constructs were purified from the soluble fractions by nickel-based affinity (Ni-NTA, Qiagen). The most concentrated fraction was further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was incubated end-over-end with 50 μl of NTA-nickel agarose beads (Qiagen) at 4°C for 30 min ...