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Citations for Qiagen :
51 - 100 of 250 citations for QT Nickel Plating Process Agent For Removing Copper Impurity since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Columns containing nickel-nitrilotriacetic acid (Ni-NTA) agarose (QIAGEN #30250) were equilibrated with 2 column volumes (CV ...
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bioRxiv - Genetics 2024Quote: ... Soluble proteins were incubated with nickel-nitriloacetic acid beads (Qiagen) for 2 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A clean-up process with DNeasy PowerClean Pro Cleanup kit (Qiagen®, Hilden, Germany) and OneStep PCR Inhibitor Removal Kit (Zymo Research® ...
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bioRxiv - Genomics 2024Quote: ... The extraction process was conducted using a QIAamp viral RNA minikit (Qiagen, CA, USA) and a QIAcube automated system (Qiagen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then removing the tail from each individual and extracting the DNA using an extraction kit (Qiagen). Following extraction ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was applied to a nickel-nitrilotriacetic acid agarose (Qiagen) column and washed with 20 mM imidazole for twice ...
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bioRxiv - Cancer Biology 2020Quote: ... the supernatant was incubated with pre-equilibrated nickel agarose resin (Qiagen) (100 ul packed resin per 50 ml culture ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen) and the histidine tag was subsequently removed by thrombin digestion (10 U/mg of protein) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was incubated with nickel-NTA agarose beads (Qiagen) for 2 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... Nickel superflow resin was purchased from Qiagen (Hilden, GE. Dithiothreitol (DTT) was purchased from Rosche (Basel ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant of cell lysates was incubated with nickel beads (Qiagen) and the eluate was then subjected to gel filtration chromatography (Superdex 200 ...
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bioRxiv - Cell Biology 2020Quote: ... lysed and incubated with nickel-nitrilotriacetic acid beads (Qiagen, Hilden, Germany) as per the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and rEDA was purified using nickel-nitrilotriacetic acid-agarose resin (Qiagen).
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bioRxiv - Biophysics 2024Quote: ... Pre-equilibrated nickel-NTA resin (Qiagen, 50 mg/mL binding capacity) was added to the clarified lysate and the lysate-resin slurry stirred in a beaker with a stir bar for 1 hour at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was incubated with nickel affinity resins (Ni-NTA, Qiagen) at 4°C for 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cleared lysates were incubated with 2-4ml nickel NTA beads (Qiagen) for 20-40 minutes before washing beads with 5-10 column volumes of lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed with 20 ml nickel affinity resin (Qiagen) and rotated at 4 °C overnight.
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bioRxiv - Molecular Biology 2023Quote: ... using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (QIAGEN, cat # 30230) and the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... HAs were purified from the supernatant using nickel-NTA agarose (Qiagen) and disposable 10mL polypropylene columns (Thermofisher) ...
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bioRxiv - Microbiology 2024Quote: ... The extraction process was executed according to the protocol of DNeasy PowerSoil Kit (Qiagen, German), and the extracted DNA was stored at -20°C for subsequent analysis.
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bioRxiv - Microbiology 2021Quote: ... total RNA was purified by removing residual DNA by an RNase-free DNase set (Qiagen, Hilden, Germany). Ribosomal RNA was removed from the DNase-treated RNA via the Ribo-Zero rRNA Removal Kits (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed by removing media from culture dishes and adding 200ul of RLT plus buffer (Qiagen) supplemented with 0.5mM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... was performed approximately 24 h after cell plating using Effectene Transfection Reagent (Qiagen) with 1 µg siRNA in 742 µL transfection mixture ...
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bioRxiv - Cell Biology 2020Quote: ... the complex was purified by nickel affinity chromatography (Ni-NTA resin, Qiagen) and eluted with elution buffer (40mM Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Protein purification was undertaken using nickel-affinity chromatography (Qiagen, Valencia, CA, USA) and endotoxin was removed by two-phase extraction with Triton X-114 22 ...
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bioRxiv - Cell Biology 2020Quote: ... the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose beads (QIAGEN) pre-equilibrated with the washing buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... and proteins were purified by two successive nickel affinity chromatography (Qiagen®) steps ...
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bioRxiv - Molecular Biology 2022Quote: ... the His-SUMO conjugates were enriched using nickel-nitrilotriacetic acid beads (Qiagen). Upon multiple washing steps ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 µL of dry nickel-nitrilotriacetic acid-agarose (Ni-NTA) beads (QIAGEN), were equilibrated with Guanidinium buffer supplemented with 5 mM β-mercaptoethanol and 50 mM Imidazole pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by purification using nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (QIAGEN). The recombinant protein was desalted with PD-10 column (SephadexTM G-25M ...
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bioRxiv - Bioengineering 2021Quote: ... His-tagged proteins were purified using a nickel column purification step (QIAGEN). Following IMAC ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified by Nickel affinity chromatography (Ni2+-NTA-sepharose resin, Qiagen). His6-FUBI-eS30 WT and AA constructs were expressed in E ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using a nickel gravity column (Qiagen, Ni-NTA Agarose) followed by a high-salt wash (1xPBS ...
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bioRxiv - Molecular Biology 2020Quote: ... added to 100 μl washed Ni-NTA agarose nickel beads slurry (Qiagen), and transferred to Snap Cap spin columns (Pierce).
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bioRxiv - Microbiology 2021Quote: ... the supernatant was extracted with a nickel-nitrilotriacetic acid-agarose suspension (Qiagen) or a Pierce GST Spin Purification Kit (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μL of dry nickel-nitrilotriacetic acid-agarose (Ni-NTA) beads (QIAGEN), were equilibrated with Guanidinium buffer supplemented with 5 mM β-mercaptoethanol and 50 mM Imidazole pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The protein was purified from the supernatant via nickel affinity chromatography (Qiagen). The protein was further purified by a Hiload 16/60 Superdex 200 column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... for GST-tagged proteins or nickel– nitriloacetic acid (Ni-NTA) agarose (Qiagen) for His-tagged proteins (2 hr ...
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bioRxiv - Biochemistry 2024Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA). Stain free gels (4-12% ...
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bioRxiv - Biophysics 2023Quote: ... GlpG in supernatant was purified using nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) affinity chromatography in 50 mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... His-tagged VndA was purified over a Nickel-NTA agarose resin (Qiagen) according to manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were purified over nickel-nitrilotriacetic acid (Ni-NTA) Agarose (Qiagen). Therefore ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant was incubated with nickel-nitrilotriacetic acid agarose resin (QIAGEN) for 3 hours at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was incubated with Ni-NTA agarose nickel-charged resins (Qiagen) that had been equilibrated in lysis buffer ...
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bioRxiv - Genomics 2024Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA), and stain-free gels (4-12% ...
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bioRxiv - Immunology 2023Quote: The RNA extraction process involved lysing mouse colonic IEC or cultured cells using RLT buffer (QIAGEN) and extracting total RNA using the RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining lysates were incubated with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was mixed with nickel-nitrilotriacetic acid agarose beads (Qiagen, Hilden, Germany) and loaded on a column ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified with nickel chelate affinity resins (Ni-NTA, Qiagen). The protein samples were collected and purified by a Superdex 200 (GE Healthcare ...