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Citations for Qiagen :
101 - 150 of 3346 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and then with (2) 15 µl Proteinase K [>600 mA/ml] and 5 µl Rnase A [100 mg/ml] (Qiagen, Germany) for 5min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Cell Biology 2023Quote: ... Spheroids were transferred into PCR-clean 1.5 mL microtubes with 100 µL of lysis buffer (LB1 from Macherey-Nagel or 700 µL of Buffer RLT (79216, Qiagen). Lysates were snap-frozen and stored at −80 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was isolated from 100 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat#69506, QIAGEN, Valencia, CA) following established protocols70 ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was isolated from frozen leaflet samples (100 mg of tissue) using the Qiagen RNeasy Plant Mini Kit (Qiagen, Valencia, CA, U.S.A) following manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was isolated from 100 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat# 69506, QIAGEN, Valencia, CA) following established protocols (Cook et al ...
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bioRxiv - Genomics 2021Quote: A half-loopful overnight grown bacterial culture was used to extract the genomic DNA using the QIAGEN Genomic-tip 100/G (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from approximately 100 mg of frozen stem tissues for each sample using the Qiagen RNeasy Plant Mini-Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Microbial sequencing was performed as previously described in.21,22 Microbial DNA was extracted from 50∼100 mg fecal material using the Qiagen DNeasy PowerSoil Pro Kit and the automated QIAcube Connect robot (Qiagen, Carlsbad CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Blood was obtained for lymphocyte preparation 3-5 days after the fifth immunization and RNA was prepared from lymphocytes using the RNeasy kit (Qiagen, Valencia, CA). A VHH-display phage library was prepared essentially as described previously [70] following each of the rounds of alpaca immunization ...
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bioRxiv - Genomics 2022Quote: ... A steel bead was added to the tube and homogenized for 30 s at 30 Hz using a Tissue Lyser II (Qiagen). Homogenate was loaded onto a DNA spin column and the flow through supplemented with ethanol was loaded onto an RNA spin column ...
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bioRxiv - Cancer Biology 2021Quote: ... was cut at the cryostate (30 cryosections of 30 μm thickness per sample) and RNA was extracted using the RNeasy Mini Kit from Qiagen and analyzed using an Agilent 2100 Bioanalyzer system ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged once more and RNA pellets were air-dried for 30-60 minutes at room temperature before resuspending in 30 μl RNase-free water (Qiagen). The quality and quantity of RNA was measured using a NanoDrop Microvolume spectrophotometer ...
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bioRxiv - Plant Biology 2024Quote: The samples were ground with two steel balls at 30 Hz for 30 s in a TissueLyser (Qiagen, Venlo, Netherlands). Next ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 30 µl RNaseA (Qiagen, Hilden, Germany). For PacBio sequencing ...
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bioRxiv - Genomics 2020Quote: ... and eluted in 30 µl elution buffer (Qiagen). Around 100 ng of purified amplicons were used for library preparation ...
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bioRxiv - Microbiology 2020Quote: ... for 3min at 30 Hz using TissueLyserII (QIAGEN). Homogenates were then clarified by centrifugation (2,000 × g at 4°C for 10 min) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and eluted in 30 μl Elution Buffer (Qiagen). In vitro transcription of gRNA templates was carried out by MEGAscript T7 Transcription Kit following the manufacturer’s protocol (Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... or 30 nM AllStars negative-control siRNA (QIAGEN). A 72-hour incubation period was used to deplete the target protein.
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bioRxiv - Evolutionary Biology 2023Quote: ... supplemented with 30 ul RNaseA (Qiagen, Hilden, Germany) prior to homogenization ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in 30 µl of elution buffer (Qiagen) and the bound DNA was eluted from the beads by heating at 98 °C for 10 min ...
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bioRxiv - Immunology 2024Quote: ... and 30 IU/ml DNAse I (Qiagen, 79254) in MACS Tissue storage solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... we adapted the same conditions used in Dip-C40 and lysed each nucleus in 150 nL of lysis buffer containing 20 mM Tris pH8/20 mM NaCl/25 mM DTT/0.15% Triton X-100/1 mM EDTA/5 µg/mL Qiagen Protease (Qiagen, cat. no. 19157). After dispensing ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μl of 20 μM siRNA were mixed with 30 μl of HiPerFect transfection reagent (Qiagen, Cat. No: 301704, Courtaboeuf, France) or Promofectine siRNA transfection reagent (PromoKine ...
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bioRxiv - Genetics 2021Quote: ... and end-point PCRs were performed following 34 cycles (94 C 30 sec, 58 C 30 sec, 72 C 1 min) using the HotStarTaq Plus DNA polymerase (Qiagen, Canada) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... one aliquot was not processed any further and one other aliquot was bead beaten with 100 mg of sterile glass beads (400-600 nm) for 30 s at 30 Hz in a Tissue-lyser II (Qiagen, Germany), in order to open up Symbiodiniaceae cells and release intracellular bacteria to facilitate their growth on agar plates ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenization was performed using a Tissue-Lyser II with stainless steel grinding balls (2 x 30s at 30 Hz) (Qiagen, USA). After 30 minutes of end-over-end at 5°C ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were then placed in pre-frozen bead beater adaptors and beaten (30/s for 30 s) in a Tissue Lyser II (Qiagen, USA). Following a brief centrifugation (20 s ...
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bioRxiv - Microbiology 2024Quote: Bacterial genomic DNA was extracted separately from caecal tissue (∼100 mg) and caecal contents (∼200 mg) using a QIAamp Fast DNA Stool Mini kit (QIAGEN, Valencia, CA, USA) following the manufacturer’s pathogen detection protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 30 mg of sample were aliquoted in Eppendorf tubes and homogenized in 400 mL of methanol for 5 min at 25 Hz with a sample disruptor Qiagen TissueLyser II Laboratory Mixer (Qiagen, Valencia, CA, USA). The homogenized mixture was centrifuged for 10 minutes at 10000g (4 °C) ...
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bioRxiv - Plant Biology 2021Quote: ... and sunflower_KTI _R (5’ – CTACTCAGATTGAACAGAAGCCAC- 3’were designed and used in a PCR reaction with total DNA extracted (DNeasy Plant Mini Kit, Qiagen, Valencia CA USA) from sunflower leaf tissue ...
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 1µg/ml Aprotinin, 1mM PMSF) using 5mm Stainless Beads and TissueLyser II (frequency: 30/sec, duration 2 × 30 sec) (Qiagen, Hilden, Germany). IL-23 was measured using either a mouse IL-23 ELISA (R&D ...
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bioRxiv - Plant Biology 2023Quote: Samples were snap frozen in liquid nitrogen and pulverised in a tissue lyser at 30 Hz for 30 secs (Qiagen TissueLyser II). Anthocyanin pigments were extracted by adding acidic methanol (1% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... and kidney of euthanized mice were weighed and homogenized 3 times for 30 s at 30 Hz using Tissue Lyser II (Qiagen, Courtaboeuf, France) in 500 µl of DMEM with 2 stainless steel beads (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... gently resuspended in 30 ml G2 lysis buffer (Qiagen), and incubated with 50ug/ml RNaseA (Qiagen ...
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bioRxiv - Pathology 2022Quote: ... eluted in 30 μl of nuclease-free water (Qiagen), and quantified using Qubit broad range RNA assay (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... using a TissueLyser II (3 min, 30 Hz; Qiagen). After vigorous mixing with chloroform at a 1:4 v/v ratio (chloroform:TRIzol) ...
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bioRxiv - Microbiology 2021Quote: ... 30 mL RNA Protect Bacterial Reagent (Qiagen, Hilden, Germany) was added to 15 mL cultures and incubated at 4 °C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... 30 μl of 10 mg/ml RNAse A (Qiagen, diluted in NK Lysis Buffer to 10 mg/ml and then stored at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 µl of 10mg/ml RNAse A (Qiagen 19101) were added to the sample ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μl of 10 mg/ml RNase A (QIAGEN 19101 ...
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bioRxiv - Immunology 2023Quote: ... 30 μl of 10 mg/ml RNAse A (Qiagen) was added to the lysed sample ...
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bioRxiv - Immunology 2023Quote: ... 30 μL of 10 mg/mL RNase A (Qiagen) was added to the lysed sample ...
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bioRxiv - Molecular Biology 2023Quote: ... for 3 min in TissueLyser II (30 Hz; Qiagen). Cell lysates were centrifuged for 15 min (4 °C ...
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bioRxiv - Physiology 2023Quote: ... using a TissueLyser II (3 min, 30 Hz; Qiagen). Lysates were centrifuged for 15 min (21,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... and eluted in 30 μL of buffer AVE (QIAGEN). Extracted RNA (7.5 μL ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µL of 10 mg/mL RNAse A (Qiagen, 19101 ...
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bioRxiv - Cancer Biology 2020Quote: ... were purchased from Qiagen (Qiagen, Valencia, CA) and qPCR was performed using the Qiagen miSCRIPT SYBR® Green PCR kit (Qiagen ...
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bioRxiv - Genetics 2022Quote: ... 5 μl of each sample was pooled into one of 5 indexed libraries and PCR purified using a QIAquick PCR Purification Kit (Qiagen, Inc., Valencia, CA, USA). Prior to preparing individuals for next generation sequencing ...