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Citations for Qiagen :
101 - 150 of 434 citations for Dioctanoyl phosphatidic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were purified over nickel-nitrilotriacetic acid (Ni-NTA) Agarose (Qiagen). Therefore ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant was incubated with nickel-nitrilotriacetic acid agarose resin (QIAGEN) for 3 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Monovalent IgG was further purified by Ni- nitrilotriacetic acid (Ni-NTA) (Qiagen) column and then size exclusion column (superdex 200 ...
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bioRxiv - Genomics 2024Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA), and stain-free gels (4-12% ...
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bioRxiv - Cell Biology 2021Quote: ... Two μg of DNA was sodium bisulphite modified using the Eptech Kit (Qiagen GmbH, Hilden, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cell pellets were resuspended in 50 mM sodium citrate buffer and treated with RNase A (QIAGEN) for 2 hours at 50°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.1 mM sodium orthovanadate and 0.2 mM phenylmethylsulfonyl fluoride (PMSF)) with a Tissue RuptorTM (Qiagen, Netherlands) homogenizer ...
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bioRxiv - Microbiology 2024Quote: ... sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using Penta-His antibody (QIAGEN)) were pooled and concentrated using Amicon filter devices with 10 kDa molecular weight cut-off (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... 1% v/v sodium dodecyl-sulfate pH 8.0) for protein extraction or in RLT buffer (Qiagen) for RNA extraction ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted from tissue and blood in salt solution using a DNeasy Blood and Tissue kit (QIAGEN Pty Ltd, VIC, Australia) or a Gentra Puregene Blood Kit (QIAGEN Pty Ltd ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from the distal half of a walking leg of each lobster using salt extraction (Aljanabi & Martinez, 1997) with an additional RNase treatment following the manufacturer’s instructions (Qiagen Inc., Toronto, Ontario, Canada). DNA quality was assessed using 1% agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μg of RNA was resuspended in 10 μl of RNAse free water and mixed with sodium bisulfite pH 5.0 (42.5 μl) and DNA protection buffer (17.5 μL) (EpiTect Bisulfite Kit, Qiagen). The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... Adapter-ligated DNA was treated with sodium bisulfite using the EpiTect Plus Bisulfite kit (QIAGEN, Hilden, Germany) and amplified using the Kapa HiFi Uracil + ReadyMix (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... The complex was initially crystallized in a buffer 0.1 M MES pH 6.5 and 0.6 M sodium fluoride from Anions Suite crystallization screen (Qiagen). After optimization of crystallization conditions ...
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bioRxiv - Biophysics 2021Quote: ... Supernatant was collected for 2.5 h binding with Ni-nitrilotriacetic acid resins (Qiagen) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... His6-AHCY was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen) using standard procedures and eluted with 50 mM Tris buffer pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining lysates were incubated with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was mixed with nickel-nitrilotriacetic acid agarose beads (Qiagen, Hilden, Germany) and loaded on a column ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...
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bioRxiv - Genomics 2021Quote: ... Nucleic acids were extracted using a QIAGEN DNeasy (DNA) kit (Qiagen Hilden, Germany). Three de novo genome sequencing methods were performed on the liver fluke ...
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bioRxiv - Microbiology 2022Quote: Nucleic acids were extracted from samples using a DNeasy PowerSoil kit (Qiagen, Germany) and quantified using the Quant-IT PicoGreen assay kit (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... DNA from plasma was extracted with the QIAamp Circulating Nucleic Acid Kit (Qiagen).
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bioRxiv - Genomics 2021Quote: ... Amino acid sequences were aligned using CLC Genomics Workbench 20.0.04 (QIAGEN, Aarhus, Denmark).
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bioRxiv - Genomics 2021Quote: Nucleic acids were extracted by homogenizing tissues using a TissueLyser II (Qiagen, 85300) followed by the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen ...
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Epigenetic alterations underlie airway macrophage differentiation and phenotype during lung fibrosisbioRxiv - Immunology 2020Quote: Nucleic acids were extracted from cells using the AllPrep Mini Kit (QIAGEN, Germany). DNA quality and quantity were assessed using Genomic DNA ScreenTape and TapeStation System (Agilent ...
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bioRxiv - Genomics 2020Quote: ... Chondrocytes were transfected in duplo with antisense locked nucleic acid (LNA) GapmeR (Qiagen) targeting P3H2-AS1 (TGAGCAACTAGGTGTA ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble recombinant protein was purified using theNickel-Nitrilotriacetic acid (Ni-NTA+; Qiagen) resin ...
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bioRxiv - Cancer Biology 2023Quote: ... all proteins were purified using Ni-NTA (nickel-nitrilotriacetic acid) resin from QIAGEN. After the incubation of Expi293 media supernatant with the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Microbiology 2023Quote: ... viral nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial nucleic acid was extracted using the EZ1 DNA tissue kit (Qiagen, Germany) on EZ1 Advanced XL (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was incubated with Ni-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germany) on a rocker for 1 hour at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 500 µL ribonucleic acid (RNA) protect bacterial reagent (Qiagen, Cat. # 76506), allowed to incubate at room temperature for 10 minutes followed by centrifugation at 5,000 x g for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... nucleic acid was extracted from cells or tissues using DNeasy mini kit (Qiagen). HIV-1 DNA was quantified by real-time PCR ...
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bioRxiv - Cancer Biology 2024Quote: Locked nucleic acid GapmeR antisense oligonucleotides (LNA ASOs) targeting LINC01432 (Qiagen, cat# 3653410) and CELF2 (Qiagen ...
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bioRxiv - Biophysics 2024Quote: ... Affinity purification was performed using nickel nitriloacetic acid (Ni-NTA) affinity column (Qiagen) attached to an AKTA™ start system (Cytiva) ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Neuroscience 2021Quote: Five hundred ng of DNA was treated with sodium bisulfite using the EpiTect Fast DNA Bisulfite kit (Qiagen). Primers for nested PCR (2 fragments for HCRT ...
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bioRxiv - Microbiology 2021Quote: ... 1mM PMSF and 0.25% sodium deoxycholate and the clarified lysate was loaded on a Ni-NTA column (Qiagen). Non-specifically bound proteins were removed by washing with lysis buffer containing 20mM imidazole and the proteins eluted with buffer containing 150mM imidazole ...
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bioRxiv - Developmental Biology 2020Quote: ... and the soluble homogenates were purified by Ni-nitrilotriacetic acid (NI-NTA) Agarose (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acids were extracted from frozen faecal aliquots using the RNeasy PowerMicrobiome kit (Qiagen). The manufacturer’s protocol was modified by the addition of a heating step at 90°C for 10min after vortexing and by the exclusion of DNA-removal steps ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was loaded on a nickel nitrilotriacetic acid (Ni-NTA) resin (Qiagen GmbH) column ...
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bioRxiv - Cell Biology 2021Quote: ... We purified the 6xHIS fusion protein on nickel-nitrilotriacetic acid agarose (Qiagen, Valencia, CA) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were extracted with the Qiagen Power Fecal Pro kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: ... and nickel-nitrilotriacetic acid (Ni-NTA) agarose resin was purchased from Qiagen (Hilden, Germany). PCR reagents ...
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bioRxiv - Cancer Biology 2020Quote: ... Nucleic acids were subsequently extracted using the AllPrep DNA/RNA Mini Kit (QIAGEN, #80204) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The enzyme was purified through a nickel-nitrilotriacetic acid column (Ni-NTA superflow, Qiagen), which was followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1.2 µM custom template-switching oligo with a Locked Nucleic Acid analog (Qiagen) at 42°C for 90 minutes ...