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Citations for Qiagen :
51 - 100 of 434 citations for Dioctanoyl phosphatidic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Columns containing nickel-nitrilotriacetic acid (Ni-NTA) agarose (QIAGEN #30250) were equilibrated with 2 column volumes (CV ...
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bioRxiv - Genetics 2024Quote: ... Soluble proteins were incubated with nickel-nitriloacetic acid beads (Qiagen) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Unmethylated cytosines were sodium bisulphite converted to uracil using the EpiTect Sulphite Kit (Qiagen), while methylated cytosines remained unchanged ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitates (IPs) were washed in high salt buffer and RNA from inputs and IPs extracted using the RNeasy micro kit (Qiagen).
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bioRxiv - Bioengineering 2022Quote: ... A high salt solution in combination with isopropanol was utilized for precipitation of RNA before purification with the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
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bioRxiv - Immunology 2024Quote: ... The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson, Bioscience, Salt Lake City, UT, USA) using the QuantiTect SYBR Green (Qiagen) and LightCycler® 480 system (Roche Diagnostics ...
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bioRxiv - Biochemistry 2022Quote: ... complexes were either concentrated using a 50 kDa MWCO concentrator and further purified on a Superose 6 Increase 10/300 column (Cytiva) equilibrated in low-salt CSFxB or loaded directly onto Ni2+ agarose (Qiagen), eluted using 300 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was applied to a nickel-nitrilotriacetic acid agarose (Qiagen) column and washed with 20 mM imidazole for twice ...
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bioRxiv - Molecular Biology 2021Quote: ... Soluble fraction was incubated with Ni-nitrilotriacetic acid (NTA)-agarose (Qiagen) beads or glutathione-sepharose (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen) and the histidine tag was subsequently removed by thrombin digestion (10 U/mg of protein) ...
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bioRxiv - Neuroscience 2022Quote: Locked Nucleic Acid (LNA) oligonucleotides for C21orf2 were purchased from Qiagen. A complete list of the used ASOs can be found in extended data table 6 ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... These kits included QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany), Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit (Norgen Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... lysed and incubated with nickel-nitrilotriacetic acid beads (Qiagen, Hilden, Germany) as per the manufacturer instructions ...
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bioRxiv - Genomics 2020Quote: cfDNA was extracted using the QIAamp Circulating Nucleic Acid kit (Qiagen). Isolation of cfDNA was done from 1 mL of plasma ...
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bioRxiv - Microbiology 2023Quote: ... The eluted nucleic acid was purified using the DNeasy Kit (Qiagen) and analyzed with RT-PCR strong stop primers (SSS ...
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bioRxiv - Cancer Biology 2024Quote: ... and rEDA was purified using nickel-nitrilotriacetic acid-agarose resin (Qiagen).
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bioRxiv - Genomics 2024Quote: ... Nucleic acid isolation was performed using the QIAcube (Qiagen, Hilden, Germany) and the PAXgene blood miRNA kit according to the manufacturer’s protocol to extract gene-encoding mRNAs ...
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bioRxiv - Microbiology 2023Quote: ... The eluted nucleic acid was purified using the DNeasy Kit (Qiagen) and analyzed by qPCR with strong-stop primers (primers PR and PU5 ...
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bioRxiv - Molecular Biology 2023Quote: ... using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (QIAGEN, cat # 30230) and the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... s-ctDNA was extracted by QIAmp Circulating Nucleic acid Kit (Qiagen) and quantified by Qubit dsDNA High Sensitivity Kit ...
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bioRxiv - Microbiology 2024Quote: ... Nucleic acid purification kits were purchased from Qiagen (Toronto, ON, Canada). Sanger sequencing for routine verification of constructs and mutant validations was done as a fee-based service by The Centre for Applied Genomics (The Hospital for Sick Children ...
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bioRxiv - Immunology 2024Quote: ... The eluted nucleic acid was purified using the DNeasy Kit (Qiagen) and analyzed with RT-PCR strong stop primers (SSS ...
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bioRxiv - Genomics 2024Quote: ... Nucleic acid extraction was performed using the Magattract HMW kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Sodium bisulfite conversion of unmethylated cytosines in DNA was based on EpiTect Bisulfite Handbook (Qiagen). We designed primers (Table S3 ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNAs were extracted from samples collected at same times from salt-treated or control plant batches using the RNeasy plus mini kit with gDNA eliminator (Qiagen, Germany). First-strand cDNAs were synthesized from 3 μg of RNAs using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Then the magnetic beads were washed in high salt buffer and RNA was extracted with the RNeasy Micro kit (Qiagen: 74004). For RNA sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... and salt treated samples (100 mM, 250 mM and 500 mM NaCl) was extracted using RNeasy Plant Mini Kit (Qiagen, Germany). RNA quality and quantity assessment ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-bead complexes were washed in high and low salt buffer and RNA was eluted from the RNA-bead complexes using the Qiagen RNeasy Plus Micro Kit (Qiagen 74034). Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230) ...
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bioRxiv - Microbiology 2020Quote: Nucleic acids were extracted using the AllPrep DNA/RNA Micro kit (Qiagen) for simultaneous purification of minute amounts of DNA and RNA from the same sample without making use of the carrier RNA ...
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bioRxiv - Cell Biology 2020Quote: ... the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose beads (QIAGEN) pre-equilibrated with the washing buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was incubated with Ni-nitrilotriacetic acid (NTA) resin from Qiagen for 1hr at RT ...
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bioRxiv - Zoology 2021Quote: Nucleic acids were extracted using a QIAamp MinElute Virus Spin Kit (QIAGEN) and used to construct the sequencing libraries ...
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bioRxiv - Microbiology 2020Quote: ... then the nucleic acid was purified using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Viral ribonucleic acid (RNA) was extracted by viral RNA kit (Qiagen, Germany), reversed by reverse transcription kit (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... Ni(II)-nitrilotriacetic acid agarose (Ni-NTA) resin was purchased from Qiagen. L-Allylglycine was purchased from Cayman Chemical Company ...
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bioRxiv - Molecular Biology 2022Quote: ... the His-SUMO conjugates were enriched using nickel-nitrilotriacetic acid beads (Qiagen). Upon multiple washing steps ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 µL of dry nickel-nitrilotriacetic acid-agarose (Ni-NTA) beads (QIAGEN), were equilibrated with Guanidinium buffer supplemented with 5 mM β-mercaptoethanol and 50 mM Imidazole pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by purification using nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (QIAGEN). The recombinant protein was desalted with PD-10 column (SephadexTM G-25M ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1 μM Locked Nucleic Acid (LNA) oligo-d(T)30 (Qiagen) in primary-probe hybridization buffer composed of 40% formamide (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acids were extracted with the QIAamp Viral RNA mini kit (QIAGEN) to be further randomly amplified using a modified Whole Transcriptome Amplification 2 (WTA2 ...
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bioRxiv - Microbiology 2021Quote: ... the supernatant was extracted with a nickel-nitrilotriacetic acid-agarose suspension (Qiagen) or a Pierce GST Spin Purification Kit (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μL of dry nickel-nitrilotriacetic acid-agarose (Ni-NTA) beads (QIAGEN), were equilibrated with Guanidinium buffer supplemented with 5 mM β-mercaptoethanol and 50 mM Imidazole pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... the supernatant was incubated with Ni-nitrilotriacetic acid (NTA) agarose resin (Qiagen) for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... for GST-tagged proteins or nickel– nitriloacetic acid (Ni-NTA) agarose (Qiagen) for His-tagged proteins (2 hr ...
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bioRxiv - Biochemistry 2024Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA). Stain free gels (4-12% ...
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bioRxiv - Biophysics 2023Quote: ... GlpG in supernatant was purified using nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) affinity chromatography in 50 mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted using nucleic acid isolation kit (AllPrep® Qiagen) according to protocol instructions ...