Labshake search
Citations for Qiagen :
101 - 150 of 2820 citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 200 mg of freeze-dried and ground roots were mixed with 1.6 mL of 1 M perchloric acid and homogenized in a TissueLyser II (Qiagen) in microtubes containing 6 glass beads of 2.8 mm in diameter ...
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bioRxiv - Genetics 2020Quote: Iron nitrilotriacetic acid (NTA) resin were prepared in-house by stripping metal ions from nickel nitrilotriacetic acid agarose resin (Qiagen) with 100 mM ethylenediaminetetraacetic (EDTA ...
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bioRxiv - Genomics 2021Quote: ... Total Nucleic Acid (TNA) was extracted using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). RNA isolation and library preparation is fully described in Butler ...
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bioRxiv - Cell Biology 2023Quote: ... snap frozen samples were homogenized in an Eppendorf tube using a 3-mm Tungsten carbide beads and TissueLyser II system (Qiagen; 4 min at 30 Hz). RNA isolation steps were followed as mentioned above in this section ...
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bioRxiv - Cancer Biology 2024Quote: ... 5’-TGGTTCTACATCAGAGTTGTT-3’ (Qiagen). Lentiviral particles expressing SMART vectors doxycyclin-inducible shRNA were from Dharmacon as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... using the QIAamp Circulating Nucleic Acid kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a nickel-nitrilotriacetic acid column (Qiagen) and then dialyzed against buffer comprised of 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... using the QIAamp Circulating Nucleic Acid kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Tris- ethylenediaminetetraacetic acid buffer was obtained from Qiagen® (Hilden ...
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bioRxiv - Cell Biology 2024Quote: ... or incubated with nickel-nitrilotriacetic acid resin (Qiagen). After washing with washing buffer the fusion constructs were eluted with elution buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: The ER-I-PpoI cells treated with 4-OHT (2.5 μM) for 1 hour were collected for extraction of the total nucleic acids using DNeasy Blood & Tissue Kits (Qiagen) or PureLink(tm ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification of p53-(1-73) from the clarified cell lysate was accomplished using a Nickel Nitrilotriacetic Acid (Ni-NTA, Qiagen) column purification and 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... viral nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany), which purifies RNA and DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The membrane was then hybridized with 50 pmol mL−1 Biotin-labeled Locked Nucleic Acid-modified DNA probes (designed and synthesized by Qiagen) specific to the 5’ end of tRNA-ValCAC and 5S rRNA ...
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bioRxiv - Cell Biology 2021Quote: ... 3-month-old zebrafish (1 fish/sample) using the RNeasy Mini Kit (Cat. No. 74104; Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Erythrocytes were lysed for 3 minutes with 3 ml of EL buffer (Qiagen). After Fc blocking for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 3’mRNA-seq libraries were prepared using QIAseq UPX 3’ Transcriptome Kit (QIAGEN). In brief ...
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bioRxiv - Cell Biology 2021Quote: ... 4 °C) and the supernatant was then loaded onto 1 ml HisTrap HP column (Qiagen) at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was incubated for 1 hour at 4°C with Ni-NTA-agarose (Qiagen), washed several times with lysis buffer and the protein was eluted by incubation for 3 hours with lysis buffer containing 500 mM imidazol ...
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bioRxiv - Genomics 2023Quote: ... Button valves were opened and biotinylated anti-pentaHis antibody (Qiagen, 1:4 dilution in HEPES) was flowed for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... additional DNA from 1-5 mL of plasma was extracted using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, catalogue # 55114). Illumina sequencing libraries were constructed using SRSLY® PicoPlus DNA NGS Library Preparation Kit (ClaretBio – Cat ...
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bioRxiv - Biochemistry 2020Quote: ... Locked nucleic acid (LNA) ASOs were ordered from Qiagen and dissolved in water to 100 μM ...
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bioRxiv - Genomics 2021Quote: ... Using the QIAmp Circulating Nucleic Acid Kit (Qiagen, 55114) we followed two of the manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleic acids were extracted using the RNeasy kit (QIAGEN). Contaminating DNA was removed using the Turbo DNA-free kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: The antisense locked nucleic acid (LNA) GapmeR (#339515, Qiagen) sequence for the Tug1 LNA was (5’-3’ ...
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bioRxiv - Microbiology 2023Quote: ... nucleic acids were extracted (QIAmp DNA Mini Kit, QIAGEN) and a multiplex nested PCR was implemented to detect known skin-tropic HPyVs (MCPyV ...
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bioRxiv - Genetics 2023Quote: ... nucleic acid was purified using QiaQuick (QIAGEN, Germantown, Maryland) and sent to Laragen for Sanger sequencing (Laragen ...
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bioRxiv - Cancer Biology 2021Quote: Whole RNA (1-3 μg total per 10 μL volume) was isolated using RNAeasy mini kit (QIAGEN) or TRIzol (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted cells were resuspended in 1 ml of Na2CO3 buffer containing 3 µg/ml RNase A (Qiagen) and incubated at 50°C for at least 4 h ...
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bioRxiv - Bioengineering 2024Quote: ... the 3×Flag-MCS fragments in LV3-SFFV-3×Flag-MCS-GFP (QZ35395, QIAGEN) was replaced by DreAM though NotI and SpeI sites to produce the LV3-SFFV-DreAM-GFP plasmid ...
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bioRxiv - Developmental Biology 2020Quote: ... rhesus fibroblast cell line (n=1), pri-CTB (n=1, rh090419) and TSCs (n=3 rh121118, rh052318, cy091318) using a FlexiGene DNA Kit (Qiagen, cat no: 51206) and quantified using a Nanodrop™ One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... siMad2 (5’-CUGAAAGUAACUCAUAAUCUA -3’) (Qiagen). For transfection of the scFv or scFvC plasmids ...
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bioRxiv - Bioengineering 2020Quote: ... Each well is then diluted with 1 to 4 v:v in RNAse free elution buffer (QIAgen) to a total volume of 8 µL ...
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bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred into the glovebox and loaded onto a gravity column in with 1 mL nickel-nitrilotriacetic acid resin (Qiagen, Hilden, Germany) that was preequilibrated with wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... beat-beading tubes were filled 1/3 with beads and soaked overnight in 500 μl buffer RLT (Qiagen). Tubes were spun at full speed and excess buffer removed ...
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bioRxiv - Cell Biology 2023Quote: ... and siNET1 #4 (Qiagen cat# SI00082040 ...
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bioRxiv - Genomics 2020Quote: ... or the QIAmp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: A PowerSoil Deoxyribonucleic Acid (DNA) Extraction Kit (QIAGEN, Hilden, Germany) was used to extract genomic DNA from fecal samples according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... coli cell lysate using Ni-nitriloacetic acid agarose resin (Qiagen) under denaturing conditions according to the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2020Quote: ... clarified lysate was applied to Ni-NTA (nitrilotriacetic acid, Qiagen) resin for 1.5h ...
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bioRxiv - Genomics 2020Quote: cfDNA was extracted using QIAamp Circulating Nucleic Acid Kit (Qiagen) with the Qiavac24s system ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated with nickel-nitrilotriacetic acid beads (Qiagen) for 2 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... and an automated nucleic acid purification system (QIAcube Connect; Qiagen). Each eluent was reverse-transcribed ...
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bioRxiv - Biochemistry 2022Quote: ... and affinity-purified using Ni-nitrilotriacetic acid (NTA) agarose (QIAGEN) as previously described (Kim et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... cfDNA was extract by QIAmp Circulating Nucleic acid Kit (Qiagen) and quantified by Qubit (Qubit dsDNA High Sensitivity Kit ...
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bioRxiv - Biochemistry 2024Quote: ... Columns containing nickel-nitrilotriacetic acid (Ni-NTA) agarose (QIAGEN #30250) were equilibrated with 2 column volumes (CV ...
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bioRxiv - Genetics 2024Quote: ... Soluble proteins were incubated with nickel-nitriloacetic acid beads (Qiagen) for 2 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... NG1 (5’ACCGACCACAGGGGG-3’) and NG2 (5’-GGTTGTAAACCTCTTTCGA-3’) and HotStarTaq® Master Mix Kit (Qiagen) up to a final volume of 25 μL ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction was incubated for 1 hour at 4 °C using a Ni-NTA resin (Qiagen). The Ni-NTA resin was washed with 4 times resin volume (RV ...