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Citations for Qiagen :
1351 - 1400 of 3116 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... and 1 μl of diluted (0.08x) QIAseq FastSelect 5S/16S/23S (Qiagen) were added into 6 μl COVID-19 specimen RNA along with 3 μl NA denaturation buffer ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using miScript II RT kit (Qiagen) as per protocol before qPCR using miScript SYBR Green kit (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1 μM Locked Nucleic Acid (LNA) oligo-d(T)30 (Qiagen) in primary-probe hybridization buffer composed of 40% formamide (Sigma ...
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bioRxiv - Genetics 2021Quote: ... for less than 1×104 cells or the RNeasy kit (Qiagen #74004) otherwise ...
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bioRxiv - Microbiology 2020Quote: ... harvested pellets were resuspended in 1 ml RNA Protect Bacteria Reagent (Qiagen), incubated for 5 min at ambient temperature ...
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bioRxiv - Microbiology 2020Quote: ... Each 1-kb fragment was purified using a gel extraction kit (Qiagen) and were ligated by PCR using primers Lpg499-DR and Lpg499-UF to generate a 3-kb fragment that was purified by a gel extraction kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM imidazole (final concentration) and 1 ml Ni-NTA resin (Qiagen) pre-washed in 25mM Hepes ...
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bioRxiv - Microbiology 2021Quote: ... multivorans 1 genomic DNA (gDNA, extracted with DNeasy Blood & Tissue Kit, Qiagen), cleaned up using the Monarch PCR Purification kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... amplicons were subjected to the QIAseq 1-Step Amplicon Library Kit (Qiagen) for Illumina compatible next generation sequencing (NGS ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... containing 10μl of 1% v/v β-mercaptoethanol TCL buffer (Qiagen # 1031576) using a BD AriaII cell sorter ...
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bioRxiv - Immunology 2020Quote: ... Tissue was homogenized at 30hz for 1 minute in a TissueLyser (Qiagen) then briefly spun at maximum RPM in a benchtop centrifuge to pellet large debris ...
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bioRxiv - Genomics 2020Quote: ... and purified from a 1% agarose gel (QIAquick Gel Extraction Kit, Qiagen).
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 1 × 106 cells using the RNeasy kit (Qiagen), and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831 ...
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bioRxiv - Microbiology 2023Quote: ... Dilutions of 104-101 plasmid copies contained 1 ng carrier RNA (QIAGEN). QPCR was performed in a LightCycler 480 Instrument II (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were homogenised in 1 ml QIAzol lysis reagent (Qiagen, Hilden, Germany) with a handheld tissue homogeniser (Appleton Woods Ltd ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using QuantiNova SYBR Green 1 Step kit (Qiagen) with 40 cycles of amplification at 57ᵒC performed on an ABI 7500 (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended with 1 mL of QIAzol (QIAGEN Inc.; Germantown, MD) and transferred to tissue homogenizing CKMix tubes 2mL (Bertin Instruments ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: THP-1 and primary macrophage RNA was extracted using RNeasy kit (Qiagen), followed by cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... HL-1 cells were transduced with a pool of 4 gapmers (Qiagen) at 40nM (10Nm each ...
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bioRxiv - Neuroscience 2024Quote: ... The frozen samples were pulverized for 1 min using a TissueLyser (Qiagen) set to 25 Hz for 1 min.
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bioRxiv - Microbiology 2024Quote: ... 1 mL of Ni-NTA agarose resin (Qiagen, 0.5 mL bed volume) was equilibrated in lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... These tissue pools were directly immersed in 1 ml QIAzol (Qiagen, Germany) and stored at -80°C until further processing ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells were harvested in 1 mL QIAzol Lysis Reagent (Qiagen, Hilden, Germany) for 5 min at RT and 200 µL chloroform (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 1 mL of Ni-NTA agarose (Qiagen) pre-equilibrated with lysis buffer via nutation at 4 °C for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... with the addition of 1% B- mercaptoethanol using the TissueLyserII (85300, Qiagen) in combination with 5mm stainless steel beads (69989 ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from 1 million cells by RNeasy columns (QIAGEN). We used 2 µg of RNA to perform reverse transcription with random primers using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was incubated with 1 mL of Ni- NTA agarose (Qiagen) for 1 h at 25 °C with rotating ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was mixed with 1 ml Ni-NTA-Agarose slurry (Qiagen) in an overhead rotator (10 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour and then transfected with siRNAs (Qiagen, Cat. No. 1027417) using lipofectamine RNAiMAX (Thermo-Fisher Scientific #13778 ...
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bioRxiv - Physiology 2023Quote: ... Agarose beads were collected in 1 ml propylene gravity flow columns (Qiagen) and washed with 10 ml Resuspension buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 200 µg mL-1 RNaseA (Qiagen, 19101), 50 µg mL-1 propidium iodide (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-His tag antibody (Qiagen, cat. no. 34650, 1:4000 dilution), respectively.
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bioRxiv - Genomics 2024Quote: ... we seeded 3000∼6000 cells to 1 QIAscout array raft (Qiagen 928031), collected individual cells into 0.2-ml PCR tubes containing 2.5μl PBS ...
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bioRxiv - Immunology 2024Quote: ... Agarose beads were collected in 1 mL propylene gravity flow columns (Qiagen) and washed with 10 mL resuspension buffer ...
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bioRxiv - Genomics 2024Quote: ... cells were collected in 1 mL of QIAzol lysis reagent (Qiagen, 79306). Nucleic acids were isolated using phenol-chloroform extraction and DNA was depleted using the Turbo DNA Free Kit (ThermoFischer ...
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bioRxiv - Biochemistry 2024Quote: ... coli medium was incubated with 1 mL Ni-NTA agarose resin (QIAGEN) for 1 h ...
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bioRxiv - Developmental Biology 2024Quote: ... obtained larval lysates were treated with 1:125 Turbo DNase (QIAGEN, 79254) according to the manufactures protocol and incubated for 10 min at 28 °C.
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bioRxiv - Molecular Biology 2020Quote: Whole blood samples from 10 healthy volunteers (n = 5 females and n = 5 males) aged from 25 to 37 were collected into PAX gene RNA blood tubes (Qiagen). Total RNA samples were isolated using QIAcube automation with the PAXgene Blood miRNA Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Oligonucleotides containing shRNA inserts were PCR-amplified with primers 5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’ and 5’-TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’ and purified with QIAquick PCR Purification Kit (Qiagen). shRNA inserts and miRE18_LT3GEPIR_Ren714 backbone (inducible via Tet-On system ...
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bioRxiv - Immunology 2020Quote: ... 5’-AGCGTCATCAGGATTGGCAA and probe 5’-TGGGTGTCTGCTTTGGAACA were used in a one-step qRT-PCR reaction with either Quantifast reagents (Qiagen) for tissue RNA or LightCycler 480 RNA Master Hydrolysis Probes (Roche ...
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bioRxiv - Immunology 2024Quote: ... and brain) homogenates collected from mice at 5 dpi and 5 dpc were generated using the Tissue Lyzer II (Qiagen). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... An 80 μL sample of the supernatant was transferred to a new tube and incubated with 5 μL of 5 mg/mL Proteinase K (QIAGEN) for 1 h at 60°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... total DNA was extracted from males (N = 5) and females (N = 5) of each genotype using the DNeasy Blood & Tissue kit following manufacturer protocol (Qiagen). Illumina libraries were prepared using the Nextera DNA Flex Library Preparation Kit (Illumina) ...
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bioRxiv - Neuroscience 2024Quote: Isolated dorsal striatal microglia (n = 5-7 per condition) were centrifuged for 5 min at 600xg and resuspended in RLT plus buffer (Qiagen) for extraction and purification of total RNA ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from dorsal mouse skin was isolated from 5 control and 5 transgenic GRK2 eKO animals with RNAEasy Mini kit (#74104, Qiagen), following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was obtained and amplified in a first-round PCR (RdRpS1 5’-GGKTGGGAYTAYCCKAARTG-3’, RdRpR1 5’-TGYTGTSWRCARAAYTCRTG-3’) using One-Step RT-PCR Enzyme MixKit (Qiagen) with the total expected size of 602 base pairs (bp) ...
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bioRxiv - Cell Biology 2024Quote: ... target sequence 5’-TTGCTTGGAGGCTACTCCCAA-3’) and control non-silencing scrambled siRNA (siSCR, target sequence 5’-AATTCTCCGAACGTGTCACGT-3’) were obtained from Qiagen. MAPK15-specific siRNA #2 (siMAPK15 #2 ...
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bioRxiv - Microbiology 2021Quote: ... and 5 mm diameter stainless steel beads (QIAGEN) were added to each sample pool ...
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... and 5-mm stainless-steel beads (Qiagen #69989) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 5 µL Proteinase K (20 mg/mL, Qiagen) and 100 µl 10% SDS (Thermo Fisher Scientific ...