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Citations for Qiagen :
1301 - 1350 of 1774 citations for 2 Triisopropylsilyl Oxazole 5 Carbaldehyde since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 2 μg of microRNA was reverse transcribed using miScript Ⅱ microRNA cDNA Synthesis Kit (QIAGEN Biotechnology Co, Ltd, China). The reverse transcription reaction system included 4 μL of 5×miScript HiFlex Buffer ...
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bioRxiv - Microbiology 2021Quote: The QIAseq SARS-CoV-2 Primer Panel paired with the QIAseq FX DNA Library construction kits (Qiagen GmbH, Germany) were used for enriching and sequencing the entire SARS-CoV-2 viral genome ...
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bioRxiv - Molecular Biology 2020Quote: ... was added to the embryos and homogenized for 2 min at 40 Hz using a Tissue Lyser LT (Qiagen) and 5 mm stainless steel beads (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from supernatants of SARS-CoV-2-infected Vero E6 cells using the RNeasy kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 2 g powdered clay samples using the PowerMax Soil DNA Isolation Kit (Qiagen, Germany) and modifications to the manufacturer’s instructions previously validated (22) ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the 2-mL culture was removed and replaced by 1 mL of RNAprotect Cell Reagent (Qiagen). After 5 min of incubation ...
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bioRxiv - Microbiology 2022Quote: ... One volume of bacterial culture containing approximately 0.2 OD600 of cells was mixed with 2 volumes of RNAprotect (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated at 6 time points (0, 0.5, 1, 2, 4, and 8 hours) using the RNeasy Mini kit (Qiagen) according to the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2021Quote: Purified ZIKV RNA and HSV-2 DNA were extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704), and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Cells were mechanically disrupted by bead-beating for 2 × 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). The samples were incubated at −80°C for 10 min and at 95°C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Nanobody PCR fragments were analyzed on 2% TAE-agarose gels and purified with the QIAquick gel extraction kit (Qiagen). PCR fragments were sequenced and individual nanobodies were cloned using the In-Fusion cloning kit (Takara Bio ...
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bioRxiv - Cancer Biology 2021Quote: ... The products were run on 2% agarose gel and extracted using the QIAquick Gel Extraction Kit (Qiagen, Cat#28706). The purified products were submitted for paired-end 300 bp sequencing on MiSeq (Illumina) ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were transfected with a mixture of 2 mg SPACA6 pMT-puro vector and Effectene transfection reagent (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 200 RPM for 2 h then approximately 1×109 CFU of bacteria were mixed at a 2:1 (vol:vol) ratio of RNAProtect (Qiagen) and incubated at room temperature for 5 min ...
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bioRxiv - Genomics 2022Quote: ... PCR#1 amplicons were selected on a 2% agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen). These amplicons were then used as template for “PCR#2” reactions ...
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bioRxiv - Genomics 2022Quote: ... PCR amplicons were size selected on a 2% agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen), and sequenced and deconvoluted using the Amplicon-EZ service provided by GENEWIZ/AZENTA (South Plainfield ...
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bioRxiv - Physiology 2022Quote: Frozen liver tissue was manually crushed and 30 mg per sample was homogenized in 1mL of 2:1 chloroform:methanol via a TissueLyser II (Qiagen). Samples were stored at 4 °C overnight with agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were isolated from a 2% agarose gel using the QiaQuick gel extraction kit (Qiagen, Cat. No. 28706) and their quality and concentration were determined using a 2200 TapeStation and D1000 screen tapes (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue was digested away from the slide by incubating the tissue with 1% 2-mercaptoethanol in RLT buffer (Qiagen) for one hour at 56°C with interval shaking ...
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bioRxiv - Developmental Biology 2020Quote: ... using the QIAGEN TissueLyser at 15 Hz for 2-3 min with a Stainless-Steel Bead (QIAGEN catalog # 69989). Phase separation was induced with chloroform ...
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bioRxiv - Systems Biology 2021Quote: RNA isolation from SARS-CoV and SARS-CoV-2 infected A549-ACE2 cells was performed as described above (Qiagen). 500 ng total RNA was used for reverse transcription with PrimeScript RT with gDNA eraser (Takara) ...
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bioRxiv - Physiology 2021Quote: ... and homogenized with metal beads (2.4 mm diameter, Askubal) for 2 min at 25 Hz in a TissueLyzerII (Qiagen). Beetles were weighed and frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein was normalized to 2 mg across all samples tested and incubated with 0.25 mL of 50% Ni-NTA resin (Qiagen) for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 g of meat or processed food powder was extracted and purified using DNeasy Mericon Food Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Libraries were constructed using QIASEQ SARS-CoV-2 Primer Panel and QIAseq FX DNA Library UDI-A kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Tissue lysates were prepared from 30 pooled 2 dpf WT and MZdnmt3aa-/- mutant by homogenization using TissueLyser LT (QIAGEN) with zirconia beads for 10 s at 50 Hz ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor, 2.5 μM template switch oligo with Qiagen #339414YCO0076714 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were harvested after 2 hours of treatment and RNA was isolated with the RNeasy Mini Kit (Qiagen #74104). RNA levels were assessed using qScript™ XLT One-Step RT-qPCR ToughMix® (Quanta Bioscience #95132-100 ...
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bioRxiv - Bioengineering 2021Quote: ... The filtered supernatant was incubated for 2 hours at room temperature with 6ml of Ni-NTA resin (Qiagen, 30210). The column containing the mix of supernatant and resin was washed four times with a washing buffer containing 20mM imidazole ...
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bioRxiv - Genomics 2020Quote: ... PCR based COVID-19 viral genome enrichment was performed using QIAseq SARS-CoV-2 Primer Panel Kit (Qiagen, Germany). A total of 98 primer pairs specific to COVID-19 were used for enrichment where primers pairs were distributed in two pools (Pool-1 and Pool-2) ...
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bioRxiv - Molecular Biology 2021Quote: The AR9 gene 226 was PCR amplified from AR9 genomic DNA and cloned into the pQE-2 vector (QIAGEN) between the SacI and SalI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 mL of samples were collected and spun at 12,000 rpm for 2 minutes and resuspended in 350 µL RNA Protect Bacterial Reagent (Qiagen). Samples were kept on ice from this point forward ...
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bioRxiv - Genomics 2021Quote: ... Illumina reads for each genome were mapped to the yeast reference genome (R64-2-1, yeastgenome.org) using CLC-Genomics software (Qiagen). Resulting read mapping files were then subjected to copy number and heterozygous single nucleotide polymorphism (hetSNP ...
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bioRxiv - Immunology 2023Quote: ... PCR products were run on a 2% agarose gel and purified using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). The obtained TCRβ libraries were quantified using the PerfeCTa-NGS-Quantification Kit according to the manufacturer’s protocol (Quantabio Inc ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of logarithmic growth phase culture was pelleted by centrifugation and resuspended by vortexing in B1 buffer (Qiagen, Hilden Germany ...
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bioRxiv - Immunology 2022Quote: ... The separated PBMCs were washed with 2% FBS and sample’s mRNA was extracted using RNeasy Plus Mini Kit (Qiagen).
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bioRxiv - Immunology 2023Quote: ... 40mM Tris-HCl ph 6.5 for 2 hour at 45°C prior to purification with QIAquick PCR Purification Kit (Qiagen). For ‘input’ DNA ...
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bioRxiv - Genomics 2023Quote: ... Obtained lists with significantly changed genes (p<0.05, fold-change ≥2) were used in Ingenuity Pathway Analysis (IPA, Qiagen).
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bioRxiv - Immunology 2023Quote: ... pH 6.0) and homogenized using a sterile 5mm stainless steel bead in a Tissue Lyser 2 (Qiagen, Hilden, Germany) for 90 seconds at 1/60Hz ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 2 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Neuroscience 2022Quote: ... Any gene with a Z-score greater than 2 was excluded from analyses.Pathway analysis was performed using Ingenuity Pathway Analysis software (QIAGEN). Genes were inputted with their FPKM (cut off >5 FPKM ...
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bioRxiv - Microbiology 2022Quote: ... 1 to 2 mL of culture were mixed with the same volume RNAprotect™ Bacteria Reagent (Qiagen, Hilden, Germany) incubated for 10 min and centrifuged ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Cell Biology 2023Quote: ... individual organoids were washed 2 times with ice-cold PBS and subsequently dissociated with 350µL of lysis buffer (RLT Buffer, QIAGEN). Samples were stored at - 80°C until they were delivered to USEQ Utrecht Sequencing Facility (Utrecht ...
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bioRxiv - Cell Biology 2023Quote: ... for 1h at 37 °C (2 units per sample) followed by purification with the RNeasy miniElute Cleanup kit (Qiagen).
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bioRxiv - Neuroscience 2023Quote: ... cells were washed 2 times with 1X PBS and RNA was isolated using a RNAeasy Plus Mini Kit (Qiagen).