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Citations for Qiagen :
1151 - 1200 of 1774 citations for 2 Triisopropylsilyl Oxazole 5 Carbaldehyde since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the bacterial strains grown in VL55 medium with 5 mM pyruvate using the Qiagen Genomic-tip 100/G DNA isolation kit and associated DNA buffers (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... we FACS-sorted single nuclei in 384-well plates prefilled with 5 μL of Vapor-Lock (Qiagen, cat. no. 981611) per well ...
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bioRxiv - Cancer Biology 2023Quote: ... of 5 million AMO1 shIRE1 cells previously treated per condition was isolated using the AllPrep DNA/RNA Mini Kit (Qiagen). gDNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA Extraction and Purification: Genomic DNA was extracted from 5 M cells using the Gentra Puregene Cell Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from Day 11 populations (n=20, 5/treatment) using Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germany) adapted from the manufacturer’s instructions to include a lysostaphin lysis step(50) ...
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bioRxiv - Immunology 2024Quote: Samples were double-sorted using a FACSAria or FACS Astrios: 600-1000 cells from each population were collected into 5 μl TCL buffer (Qiagen) containing 1% beta-mercaptoethanol (β-ME ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was thoroughly aspirated and replaced with 20 µl of 1x PBS containing 5 mg/ml of RNase A (Qiagen) and 12 mM of purified H6-mNeonGreen-NLS protein as a loading control ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from 5×106 sorted WSU-FSCCL IgG+ and IgM+ cells using the RNeasy mini kit (Qiagen, 74004). RNA quality control was performed using a Bioanalyzer (Agilent) ...
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bioRxiv - Genomics 2024Quote: Total RNA was isolated from 5 × 106 cells harvested from frozen patient vials using the RNeasy Plus mini-Kit (Qiagen). Libraries were prepared with the NEBNext® rRNA Depletion Kit and were sequenced using the Illumina NextSeq500 platform ...
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from the anterior half of the body from 10-12 5 dpf larvae per sample at using the Rneasy Mini Kit (Qiagen). cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... the anterior segment was pelleted by centrifugation for 5 minutes at 600g and then subjected to total RNA extraction using a RNeasy Mini Kit (QIAGEN). Following RNA extraction ...
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bioRxiv - Genomics 2024Quote: ... Each sample was mixed with 5 µL of one of 6 Illumina adapter sequences (specified in the Qiagen protocol appendix) and 2 µL of ultralow input ligase ...
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bioRxiv - Systems Biology 2024Quote: ... for 5 minutes and immediately lysed in the β-Mercaptoethanol-containing lysis solution from the RNeasy minikit (Qiagen Cat # 74104). RNA was extracted following the Qiagen kit instruction manual and eluted in 10mM Tris-HCl ...
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bioRxiv - Physiology 2024Quote: Tissues were disrupted using stainless steel beads of 5 mm diameter in a Tissue Lyser LT (Qiagen, Venlo, The Netherlands). The resulting tissue homogenates were purified using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µl of serum was centrifuged at 2000 x g for 5 min to remove debris and processed using the DNeasy Blood and Tissue Kit (Qiagen), with the ethanol precipitation step performed overnight at -20°C ...
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bioRxiv - Genomics 2024Quote: ... The pooled cells were centrifuged at 500 × g for 5 minutes at 4°C and resuspended in 100 μl of buffer EB (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... SF was transfected with 12.5 nM antisense LNA GapmeRs CBP (Qiagen, Sequence: 5′-GCG GCG ATC CTT TAG A-3′) or p300 (Qiagen ...
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bioRxiv - Immunology 2024Quote: Parasite DNA quantification by qPCR was conducted on genomic DNA extracted from 5% of every brain homogenate using the DNEasy Blood & Tissue Kit (QIAGEN). As previously outlined (58) ...
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bioRxiv - Genomics 2024Quote: ... RNA was first amplified using a first-round PCR (RdRp S1 5ߣ-GGKTGGGAYTAYCCKAARTG -3’, RdRp R1 5’-TGYTGTSWRCARAAYTCRTG-3’) with the One-Step RT-PCR Enzyme MixKit (Qiagen), targeting a total expected size of 620 base pairs (bp) ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from 5 IGH+ and 7 IGHUND lymphoma cases using either QIAamp DNA FFPE Tissue Kit (QIAGEN) (FFPE tissues ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from input and IP samples by incubation with 1 mL Trizol at room temperature for 5 minutes and then RNeasy Lipid Tissue Mini Kit (Qiagen) via the manufacturer’s protocol with on-column DNase treatment (Roche) ...
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bioRxiv - Zoology 2024Quote: ... Multiplex PCR was performed in 10 µl reaction volume containing 5 µl 2x QIAGEN Multiplex PCR Master Mix (QIAGEN, Germany), 2 µl 5x Q-Solution (QIAGEN) ...
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bioRxiv - Cancer Biology 2024Quote: ... and homogenised in NP40 lysis buffer (as described previously) using mechanical shearing with 5 mm stainless steel metal beads (Qiagen). Debris were cleared by centrifugation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mL of stabilization mix (RNAprotect Bacteria Reagent - Qiagen diluted with PBS in a 2:1 volume ratio) was applied on plates ...
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bioRxiv - Developmental Biology 2021Quote: ... RNAs were isolated from 6-month H19ΔICR/H19ΔICR and H19+/H19+ littermates (2 per genotype) using RNeasy Plus Mini Kit (Qiagen). Samples with RNA Integrity numbers >9 were Ribosomal RNA depleted using RiboZero Gold Kit (Illumina) ...
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bioRxiv - Neuroscience 2020Quote: ... The 205bp amplicon from PCR 2 was gel extracted using the MinElute gel extraction kit (Qiagen Cat#: 28606), and sent to Genewiz for Amplicon-EZ targeted deep sequencing and Sanger sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... We pooled 2-3 fishes (6 weeks old) and extracted whole RNA using the RNAEasy Micro kit (Qiagen), with blood ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR #2 products were validated using gel electrophoresis and purified using the QIAquick Gel extraction kit (QIAGEN). The purified libraries were then quantified by Nanodrop and Qubit prior to sequencing on an Illumina MiSeq.
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bioRxiv - Systems Biology 2021Quote: ... Differences between samples and controls were calculated based on the 2−ΔΔCT method using RNU6 control primer (Qiagen) as normalizer ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from 2-week-old Arabidopsis seedlings using an RNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... single cell directly into 2 µl lysis buffer (30 mM Tris-HCl [pH = 8.0], 10 mM NaCl, 0.2 µL Proteinase K [Qiagen, #19133] ...
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with Penta His Alexa Fluor 647 Conjugate (Qiagen, UK). Hits (duplicate AF647 positive spots ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... The soluble fraction was split between two columns each with 2 ml Ni-NTA agarose beads (Qiagen, 30210) pre-equilibrated with Lysis Buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2022Quote: ... The upper aqueous phase containing RNA was carefully transferred to a 2 ml collection tube (cat. 990381, Qiagen) without touching the interphase and placed in a QIAcube instrument for extraction.RNA extraction was carried out using the recommended protocol (FIW-50-001-J_FW_MB and PLC program version FIW-50-002-G_PLC_MB ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Plant Biology 2020Quote: ... The clear supernatant was then gently mixed with 1 ml of Ni+2-NTA agarose beads (Qiagen, Germany) for 30 min and then added to the column with a flow rate of 0.5 ml/min ...
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bioRxiv - Genomics 2020Quote: Total RNA of 2-3 million cells was isolated using miRNeasy Kit according to the manufacturer’s instructions (Qiagen). The quality of the RNA was assessed by a standard sensitivity NGS fragment analysis kit on Fragment Analyzer (Advanced Analytical Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Both barcode and digested fragment products were run on a 2% gel and purified by gel extraction (Qiagen). NGS library was sequenced using an Illumina MiSeq and Illumina v3 MiSeq Reagent Kits with 150 base pair single-end sequencing according to standard manufacturer protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were purified from 2% agarose gels via the QIAqick gel extraction kit (QIAGEN cat.#28704) and subjected to next-generation sequencing on a HiSeq instrument lane (Illumina ...
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bioRxiv - Physiology 2020Quote: Purified total RNA was extracted from fresh BM-MSCs at passage 2 using a Qiagen Minikit (Qiagen, Australia). Quantitative RT-PCR was performed (n=3 mice/group ...
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bioRxiv - Physiology 2020Quote: ... Groups of five flies were transferred to 2 mL tubes and were homogenized in 100μl PBS + 0.1% Triton X-100 using a TissueLyser LT (Qiagen). The samples were then spun down at 12,000 rpm and the supernatant transferred to fresh vials and the absorbance of each sample at 603 nm was measured using a NanoDrop ...
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bioRxiv - Genomics 2021Quote: Total RNA was collected from 2×106 CAR T cells with the RNEasy Plus Mini isolation kit (Qiagen). Library preparation and RNA-seq was performed by BGI America (Cambridge ...
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bioRxiv - Biochemistry 2021Quote: ... brucei genomic DNA was isolated from ∼ 2 × 108 bloodstream form cells using a DNeasy Blood & Tissue Kit (Qiagen) using standard methods.
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bioRxiv - Genomics 2021Quote: ... cfDNA was isolated from 2 ml of plasma using either the manual QIAamp circulating nucleic acid kit (Qiagen), or the semi-automated QIAsymphony DSP Circulating DNA Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... for the SARS-CoV-2 low stringency screen with the QIAmp DNA Blood maxi kit (Qiagen ref. 51194). In a first PCR step ...
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bioRxiv - Microbiology 2021Quote: Frozen plant tissue was ground using the TissueLyser II (QIAGEN; 2 cycles of 30 seconds at 27 Hz) and 3-mm zirconium oxide beads (Glen Mills Inc.) ...
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bioRxiv - Immunology 2021Quote: RNA of cells exposed to SARS-CoV-2 was isolated with the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA was extracted from 2-3 embryos pooled per genotype using the RNeasy Mini Kit (QIAGEN, 74106) and QIAshredder columns (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... using a MiSeq reagent kit v3 with 2 × 75 paired end-reads and sequence analysis was performed using CLC-Genomics workbench (v10.1.1., Qiagen). Enrichment in reads of each sample was compared to the corresponding control and mapped as peaks against the annotated genome of S ...