Labshake search
Citations for Qiagen :
1201 - 1250 of 1301 citations for Recombinant Human GPC3 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
-
bioRxiv - Synthetic Biology 2021Quote: ... After spinning down the insolubilized protein fraction the supernatant was loaded onto an equilibrated mini NiNTA spin column (Qiagen) and the flow-through was reloaded twice to maximize protein binding ...
-
bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
-
bioRxiv - Genomics 2022Quote: ... DNA and RNA was extracted from fresh-frozen tissue samples using the AllPrep DNA/RNA/Protein minikit (Qiagen, USA) as per the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... Recombinant SlERF.D7-6XHIS protein was purified by Ni2+gravity flow chromatography according to the manufactureer’s protocol (Ni-NTA Agarose, Qiagen) as described ...
-
bioRxiv - Plant Biology 2022Quote: ... Recombinant RIN-6XHIS protein was purified by Ni2+ gravity flow chromatography according to the manufacturer’s protocol (Ni-NTA Agarose, Qiagen). For the EMSA assay ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from FACS-purified astrocytes from a single mouse brain using the QIAshredder and the All-prep DNA/RNA/protein Mini Kit according to the manufacturer’s protocol (Qiagen). Total RNA concentration was measured using a NanoVue PlusTM spectrophotometer (Biochrom) ...
-
bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
-
bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from these samples according to the manufacturer’s instruction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). RNA was quantified using a Nanodrop-1000 spectrophotometer (Thermo-Scientific ...
-
bioRxiv - Genetics 2023Quote: ... Precipitated proteins were resuspended in 6M guanidine hydrochloride and incubated with 25 µL of Ni-NTA agarose beads (Qiagen) overnight ...
-
bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were analyzed by NTA and then processed with AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen).
-
bioRxiv - Cancer Biology 2023Quote: ... DNA was isolated using the SampleIN Direct PCR Kit (highQu) or the AllPrep DNA/RNA/Protein Mini Kit (Qiagen), and amplified with ALLin HS Red Taq Mastermix (highQu ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA from hippocampus and pre-frontal cortex was purified with AllPrep DNA/RNA/Protein Mini Kit (QIAGEN, 80004) and DNAseI digested (QIAGEN ...
-
bioRxiv - Biophysics 2021Quote: The nsp10:16 protein complex was supplemented by 1 mM sinefungin Crystals of the nsp10:nsp16 protein complex grew in three days in commercial JCSG I-IV screens (Qiagen). They were cryo-protected in mother liquor supplemented with 20% glycerol and flash frozen in liquid nitrogen ...
-
bioRxiv - Biophysics 2021Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the two assay plasmids by temperature-cycle ligation.
-
bioRxiv - Cell Biology 2022Quote: ... were performed on significantly altered proteins in CAVIN1-null cells using the “core analysis” function included in the Ingenuity Pathway Analysis (IPA) software (QIAGEN Bioinformatics ...
-
bioRxiv - Molecular Biology 2020Quote: RNA and protein were extracted from cell lines using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA, USA). Total RNA was also DNase treated using the TURBO DNA-free Kit (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2020Quote: RNA was extracted simultaneously from the same tissue pieces used for DNA extraction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... Approximately 20 mg of jejunum were mixed with 600 μL of the prepared protein lysis buffer and homogenized using 5 mm stainless steel beads (Qiagen) and a TissueLyser II system (Qiagen) ...
-
bioRxiv - Microbiology 2019Quote: ... coli BL21 (DE3) as fusion proteins containing an N-terminal His6- tag and purified by immobilized metal-affinity chromatography (Ni-NTA, Qiagen). Full-length DosR was also expressed with N-terminal GST-tag ...
-
bioRxiv - Molecular Biology 2019Quote: ... protein samples were processed further according to the Western Blot protocol and RNA was extracted using an RNAeasy Kit (Qiagen).
-
bioRxiv - Biochemistry 2021Quote: ... The his-tagged 3C protease was removed by flowing and washing off cleaved transporter protein over two 100 µl beds of Ni-NTA Superflow resin in tandem (Qiagen). Eluent was concentrated using 100 kDa cut-off Amicon Ultra filters (Millipore-Sigma).
-
bioRxiv - Microbiology 2021Quote: ... Proteins were purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). Polyclonal rabbit antisera were raised by Covance Research Products Inc ...
-
bioRxiv - Biochemistry 2020Quote: Initial crystals of Nup133NTD-VHH-SAN5 were obtained at 18°C in 1 day as part of the Protein Complex suite (Qiagen) in a 96-well sitting drop tray with a reservoir containing 15% PEG MME 2,000 ...
-
bioRxiv - Biochemistry 2021Quote: ... Cell debris and protein aggregates were removed by centrifugation and the supernatant was loaded on a Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with lysis buffer and the protein eluted with lysis buffer containing 200 mM imidazole ...
-
bioRxiv - Cell Biology 2021Quote: Genomic DNA from 1×106 FACS sorted p7 MPs from one animal was isolated with the All Prep DNA / RNA / Protein Mini Kit (Qiagen) and used as one sample ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell debris and protein aggregates were removed by centrifugation and the supernatant was loaded on a Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with lysis buffer and the protein eluted with lysis buffer containing 200 mM imidazole ...
-
bioRxiv - Cell Biology 2021Quote: ... Genomic DNA of clones showing depletion of spartin protein by immunoblot analysis with the spartin antibody were extracted (DNeasy Blood and Tissue Kit, Qiagen), and the genomic DNA sequence surrounding the target exon of spartin was amplified by PCR (sense ...
-
bioRxiv - Molecular Biology 2020Quote: Genomic DNA from CRISPR-on HEK293T cells and control cells were purified using a AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Then ...
-
bioRxiv - Microbiology 2020Quote: ... The soluble fraction containing the protein of interest was obtained by centrifugation at 12000xg for 20 min and was mixed with Ni2+-NTA beads (Qiagen) for 1.5 h by rotation at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were washed 3 times with sterile 1x PBS and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed four times.
-
bioRxiv - Immunology 2022Quote: ... Plasmids encoding the recombinant full-length Spike protein and the RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). ELISA plates (Immulon 4 HBX ...
-
bioRxiv - Biochemistry 2022Quote: ... the samples were centrifuged 15,000 x g at 4°C for 30 min and the resulting supernatant containing the target protein was filtered and purified using a pre-equilibrated Ni-NTA beads column (Qiagen). The recombinant protein was eluted using 4 mL of elution buffer (8 mM Na2HPO4 ...
-
bioRxiv - Biochemistry 2021Quote: ... imidazole (20 mM final) was added to the eluted protein which was subsequently incubated with 5 mL NiNTA resin (Qiagen) at 4 °C overnight with constant inversion ...
-
bioRxiv - Neuroscience 2020Quote: ... total RNA was extracted from AAV1-transduced primary hippocampal neurons (DIV12-14) using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Total RNA was polyA selected and sequenced (Hudson Alpha GSL ...
-
bioRxiv - Biochemistry 2019Quote: Mutant Chinese hamster BiP proteins with an N-terminal His6-tag were purified as described before with modifications (Preissler et al, 2017b). Proteins were expressed in M15 Escherichia coli (E. coli) cells (Qiagen). The bacterial cultures were grown in LB medium supplemented with 100 µg/ml ampicillin and 50 µg/ml kanamycin at 37 ° C to an OD600nm of 0.8 and expression was induced with 1 mM IPTG ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membranes containing the channels were collected by centrifugation (100,000×g) and proteins were extracted with 40 mM DM.65 Channels were purified using Ni-NTA agarose beads (Qiagen). Liposome reconstitution was performed using E ...
-
bioRxiv - Bioengineering 2021Quote: The dataset of differentially abundant proteins between groups were classified for cell compartment association with Ingenuity Pathway Analysis (IPA, Qiagen) and for matrisome categories using The Matrisome Project database,[28] ...
-
bioRxiv - Cell Biology 2021Quote: ... Identified interacting proteins were analyzed through the use of QIAGEN’s Ingenuity® Pathway Analysis (IPA®, QIAGEN, Redwood City, CA) and UniProt online protein knowledgebase (http://www.uniprot.org/).
-
bioRxiv - Cell Biology 2021Quote: The GST fusion proteins were expressed in bacteria (E. coli, BL21) and purified by glutathione-Sepharose (Cat No. 27-4574-01, Qiagen) as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Plasmids encoding the recombinant full-length Spike protein and the RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). ELISA plates (Immulon 4 HBX ...
-
bioRxiv - Molecular Biology 2021Quote: The assessment of ligand binding was performed via shifts in protein thermostability upon binding using thermal denaturation on a rotary quantitative PCR (qPCR) instrument (Qiagen Rotor-Gene Q 2plex HRM ...
-
bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
-
bioRxiv - Immunology 2021Quote: ... Crystals of SB16-RBD and SB45-RBD complexes and Sb16 alone were observed within one week using Protein Complex (Qiagen) and Wizard Classic 4 (Rigaku) ...
-
bioRxiv - Cell Biology 2020Quote: The validated Alx1DN (N-terminal portion of protein product containing homeodomain and nuclear localization domains) clones in pCS2+8 were purified via miniprep (Qiagen) alongside a control (C-terminal portion containing transactivation domain) ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
-
bioRxiv - Cancer Biology 2022Quote: ... 250,000 cells were lysed in RLT-plus buffer and RNA was purified with AllPrep DNA/RNA/Protein Mini Kit (#80004, Qiagen) according to the manufacturer protocol ...
-
bioRxiv - Biophysics 2022Quote: ... membranes were solubilized in 1.2% N-decyl-β-maltoside (DM) and the protein was purified on a Ni-NTA affinity column (Qiagen). Following His-tag removal ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...