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Citations for Qiagen :
801 - 850 of 1301 citations for Recombinant Human GPC3 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The fusion protein was affinity purified using Ni-TNA agarose (Qiagen). Details are provided in the Supplementary Information methods section.
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bioRxiv - Microbiology 2022Quote: ... Protein was purified under native conditions according to the QIAexpressionist (Qiagen) protocol ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen), eluted with 50 mM Tris-base ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). To purify JetAB complexes containing one full-length and one truncated JetA protomer ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified from sonicated lysates using Ni-NTA resin (Qiagen) and gravity flow ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris gels (Novex, Qiagen) using MOPS running buffer (Novex ...
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bioRxiv - Developmental Biology 2023Quote: ... After the protein digestion with Proteinase K (QIAGEN, Cat. No. #158920) and RNA digestion with RNase A (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... and AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen). Isolated DNA and RNA samples were quantified by Nanodrop (ThermoFischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen) and a gravity column (Biorad) ...
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bioRxiv - Biochemistry 2023Quote: ... The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Biophysics 2024Quote: SNAP-GAF protein was purified on Ni-NTA agarose (Qiagen 30210), followed by Amylose resin (NEB cat ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were purified using a two-column protocol: Ni-NTA (Qiagen) affinity chromatography followed by size exclusion chromatography (HiLoad Superdex 75 pg or 250 pg ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequence reads were mapped against the human reference genome (NCBI build 37/hg19) using CLC Genomics Workbench (version 5.0.1) software (QIAGEN). Mutation analysis was performed under recessive ...
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bioRxiv - Microbiology 2019Quote: Microbial genomic DNA was extracted from the human stool samples using the DNeasy PowerSoil DNA Isolation Kit (Qiagen). The V4 region of 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform(67) ...
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bioRxiv - Molecular Biology 2019Quote: ... in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT) (RT2 qPCR Primer Assays, Qiagen) mRNA when performing siRNA analyses.
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bioRxiv - Genetics 2019Quote: ... The list of human orthologs was analyzed for pathway enrichment by Ingenuity Pathway Analysis software (Qiagen, Hilden, Germany).
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bioRxiv - Cell Biology 2019Quote: ... transfected with 100 ng of human TfR (and 100ng of rescue construct if applicable) using the Effectene (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... human HDL (40nM) or PBS for 48 hours prior to RNA isolation using the RNeasy Mini kit (Qiagen). RNA samples were converted to cDNA libraries by the Northwestern University Genomics Core facility and were then run on the Illumina HT-12 microarray ...
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bioRxiv - Microbiology 2021Quote: ... Cell-free cfDNA (both human and mouse) was isolated with QIAamp DSP Circulation NA Kit (QIAGEN, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 22 human vaginal samples using the QIAamp UCP Pathogen Mini Kit (QIAGEN, Venlo, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: HeLa cells (2×106) were transfected with 100 pmol of validated siRNAs against all human ZDHHCs [42] (Qiagen) using interferrin transfection reagent (Polyplus) ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA from murine or human monocytes was isolated using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, 80224). DNA (1 µg ...
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bioRxiv - Neuroscience 2021Quote: The total RNA from mouse cortex and human PBMC was extracted using the RNeasy® Mini Kit (Qiagen) and real-time PCR was done as described in the Supplementary material.
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bioRxiv - Neuroscience 2020Quote: ... RNA was isolated from human autopsy brain tissue using the RNeasy Plus Universal Mini Kit (Qiagen Cat# 73404).
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from cells or human or mouse brain tissues using RNeasy Mini kit (Qiagen, 74106). Reverse transcription was carried out using iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... knockdown of KCNB1 expression in human cells was carried out using a mixture of 4 siRNA duplexes (Qiagen; Cat# ...
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bioRxiv - Immunology 2020Quote: ... RT2 profiler PCR array of human NF-κB signaling targets was performed as instructed by the manufacturer (Qiagen).
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bioRxiv - Genomics 2021Quote: ... The manufacturer’s protocol was followed for manual purification of total RNA from human whole blood (Qiagen, cat #762164). RNA was eluted with RNase free water ...
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bioRxiv - Neuroscience 2021Quote: Microdissected mouse and human brain tissue was flash frozen and homogenized in Trizol using a TissueLyser II (Qiagen). Phenol chloroform extraction was then used together with the RNeasy mini kit (QIAGEN ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR was performed using QIAGEN’s RT2 Profiler™ PCR Array Human Cell Death PathwayFinder (QIAGEN, Hilden, Germany). RT-PCR data was analyzed and visualized for pathway analysis by GeneSpring GX 14.9 (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from the human biopsy samples and mice colon tissue was isolated using RNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Reads from *.fastq files were mapped to the human reference genome (GRCh37) using CLC Genomics Workbench 12.0 (Qiagen). Transcript abundance was expressed as reads per kilobase of transcript per million mapped reads (RPKM) ...
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bioRxiv - Cancer Biology 2024Quote: ... Human myeloma cell lines were authenticated by subjecting genomic DNA isolated with the QIAamp DNA Mini Kit (Qiagen), and short tandem repeat (STR ...
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bioRxiv - Neuroscience 2023Quote: ... Bulk gDNA and RNA from human retinal organoids were isolated using the AllPrep DNA/RNA Micro Kit (Qiagen) or DNeasy Blood & Tissue Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: SiRNAs targeting human MSI2 (Supp Table S3) and nonspecific control pool siRNAs were purchased from Qiagen (Frederick, MD). Cultured cells at 50% confluence were transfected with siRNA at final concentrations of 50 nmol/L using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from human glioblastoma cells using the RNeasy kit according to the manufacturer’s instructions (Qiagen). RNA quality was assessed on a Bioanalyzer (Agilent ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from the frozen human stomach tissues using the RNeasy plus Mini Kit (Qiagen, US) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reads were aligned to the GRCm38 build of the human genome by using CLC Genomics Workbench v10 (Qiagen).
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bioRxiv - Neuroscience 2023Quote: Total RNA was collected from treated human or murine primary spinal cord astrocytes using an RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Primary human Fibroblasts were washed with PBS before total RNA extraction using the RNeasy Plus Mini Kit (Qiagen).
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bioRxiv - Genomics 2024Quote: Genomic DNA from human CD34+ cells from three normal adult donors was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: RNA from human cell cultures was extracted from 6 well plates using RNeasy Plus micro kit (Qiagen, 74034). The plates were washed once with PBS ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted from human CSF samples using the PAXgene® Blood miRNA kit (Pre-Analytix, Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... All proteins were purified by affinity chromatography on Ni-NTA agarose (Qiagen), followed by incubation with TEV protease at 4 °C coupled with dialysis into buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... Functional analysis of differentially abundant proteins was performed with IPA (QIAGEN Inc.)[14] ...
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bioRxiv - Microbiology 2021Quote: ... 100 µL of washed magnetic beads (BioMag Protein A, Qiagen, Hilden, Germany) resuspended in 100 µL TN-buffer were added to each sample (total volume of 750 µL ...
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bioRxiv - Immunology 2020Quote: ... Protein purification was undertaken using nickel-affinity chromatography (Qiagen, Valencia, CA, USA) and endotoxin was removed by two-phase extraction with Triton X-114 22 ...
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bioRxiv - Molecular Biology 2020Quote: Total protein was extracted using the Qiagen All prep mini kit (Qiagen) after elution through the RNA spin column ...
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bioRxiv - Cell Biology 2019Quote: ... Both proteins were affinity purified using Ni-NTA agarose beads (Qiagen, 30250) followed by gel filtration with a Superdex 200 HiLoad 16/600 column (GE Healthcare ...