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Citations for Qiagen :
1151 - 1200 of 1336 citations for Lassa Fever Virus GP2 Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: All proteins from previously frozen samples (tumors and healthy parts of murine colon) were extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Protein concentrations were estimated using NanoDrop A280 absorbance ...
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bioRxiv - Plant Biology 2023Quote: ... This clarified extract was used for the purification of the His-tagged proteins using Ni-NTA agarose resin (Qiagen, USA). The agarose resin was washed twice by resuspending in lysis buffer followed by centrifugation at 3000 rpm and removing the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... The protein was purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). A polyclonal rabbit antiserum was raised by Labcorp Early Development Laboratories Inc ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and proteins were extracted from fresh or snap-frozen FACS-sorted 2-5 million splenic B cells using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Approximately 25 mg of frozen tissues were transferred to 2 mL Eppendorf Protein Lobind tubes containing one 5 mm stainless steel bead (cat# 69989, Qiagen) and 500 µL of lysis buffer consisting of 5% SDS in 50 mM TEAB with protease inhibitors cocktail (cat# A32953 ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Cell Biology 2023Quote: ... significantly regulated human proteins (t-test, p-value ≤ 0.01; described above) were characterized by core analysis in IPA® software (version 68752261, QIAGEN Inc. ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Tris–HCl 20 mM (pH 7.5)) by sonication and the His-tagged proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole in buffer A but complemented by ATP 1 mM + MgCl2 3 mM in the case of the Vibrio cholerae helicase ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved sP36 protein was afterwards purified by a second step of affinity using Ni-NTA agarose (Qiagen, Hilden, Germany) in phosphate buffer A.
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bioRxiv - Plant Biology 2023Quote: ... The recombinant protein was partially purified by affinity chromatography on nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com). Purification steps were carried out as per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and 20 mM imidazole.[50,51] The cells were disrupted via probe sonication (Brandson) and the protein was purified with Ni-NTA affinity chromatography (Qiagen). The nanopores were eluted from the beads with 3× 250 µL fractions of elution buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The protein was purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). A polyclonal rabbit antiserum was raised by Labcorp Early Development Laboratories Inc.
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bioRxiv - Physiology 2021Quote: Lyophilized mouse hearts were homogenized in RIPA buffer supplemented with protease and phosphatase inhibitors (Pierce) using a TissueLyser II apparatus (Qiagen), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C) ...
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bioRxiv - Physiology 2021Quote: 150-200 islets from each mouse islet preparation were washed with PBS and used to generate RNA samples via the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: DNA for 16S rRNA gene sequencing was extracted from 10-50 mg fecal pellet from each mouse using the DNeasy Powersoil HTP 96 Kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from frozen mouse liver (20-25 mg) and HepG2 cells (5-6 million) using the AllPrep DNA/RNA Mini Kit (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse tissue disruption and homogenization was performed in RIPA buffer supplemented with 1:100 protease using Tissue Lyser LT (Qiagen), for 5 min at 50 Hz ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from mouse hippocampi was extracted with the RNeasy mini kit according to the manufacturer’s instructions (Qiagen, Courtaboeuf, France). RNA quality and concentration were assessed by spectrophotometry with the NanodropTM apparatus (Thermoscientific ...
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bioRxiv - Cell Biology 2019Quote: The decellularized Balb/c mouse pancreas scaffolds were washed in PBS and homogenized-lysed for 1 hr in Tissue homozilyser II (Qiagen). The ECM proteins were dissolved in lysis buffer containing Tris HCl 0.06M ...
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bioRxiv - Neuroscience 2020Quote: ... including miRNA was isolated from brains of E11 mouse fetuses (3 biological replicates) at 2 h after irradiation using the miRNeasy Mini Kit (Qiagen). RNA was subsequently processed for hybridization to GeneChip miRNA 4.0 microarrays (Affymetrix ...
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bioRxiv - Bioengineering 2020Quote: ... The prepared cDNA was preamplified using the RT2 PreAMP Primer Mix for Human and Mouse PCR Array (Qiagen, PBH-181Z). cDNA was analyzed by RT-qPCR using a Qiagen RT2 profiler custom panel (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... RNA-sea FASTQ data were processed and mapped to the mouse reference genome (GRCm38) using CLC Genomics Workbench 20 (Qiagen). Differential gene expression was performed using the DESeq2 package in R43 ...
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bioRxiv - Bioengineering 2019Quote: RT-PCR was carried out as described previously.27 Total RNA was purified from the mouse retinas using the miRNeasy plus mini kit (Qiagen) and reverse-transcribed with SuperScript III (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... we also probed for the Strep-tag on γTuNA (1:1000 dilution of Strep-tag mouse monoclonal antibody, cat. # 34850, Qiagen). Band intensities were measured in ImageJ and normalized in Prism 7 by the wildtype γTuNA band (positive control) ...
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bioRxiv - Microbiology 2022Quote: ... RNA-seq FASTQ data were processed and mapped to the mouse reference genome (GRCm38) using CLC Genomics Workbench 20 (Qiagen). Differential gene expression was performed using the DESeq2 package in R34 ...
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bioRxiv - Microbiology 2022Quote: RNA extracted from mouse lungs as described above was cleaned up using On-Column DNAse using RNAse-Free DNase Set (Qiagen) and RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... was used for human ISG15 and ISG56 and mouse genes including Gapdh as the housekeeping gene on the Rotor-Gene Q 5plex (Qiagen). RT-qPCR primers and probes are listed in the supplementary table 2.
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bioRxiv - Bioengineering 2022Quote: ... The mouse genomic DNA was extracted and purified from the whole brain from C57BL/6N mouse by using DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2021Quote: ... DRG neurons were transfected in serum free DMEM solution with predesigned fluorescently labelled siRNA directly against mouse TMEM120A (TACAN) from Qiagen-FlexiTube (cat ...
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bioRxiv - Cell Biology 2021Quote: Gene expression studies were conducted on RNA extracted from PTEC cell pellets or from 30-50mg of mouse kidney cortex using the RNAeasy Mini Kit (Qiagen) (see Supplemental Methods) ...
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bioRxiv - Neuroscience 2020Quote: ... Fold enrichment for each primer set was normalized to input and reported relative to a negative control gene desert region (mouse Igx1a (Qiagen). Primers ...
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bioRxiv - Immunology 2021Quote: ... 150 ng DNA per reaction was amplified in duplicate using primers and probes specific to γHV68 Orf50 and mouse Ptger2 (see table) and 2× QuantiNova Probe Mastermix (Qiagen). Standard curves were obtained by serial dilutions of Orf50 and Ptger2 gBlocks (ORF50 ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Isolated genomic DNA (500 ng) of E8.5 mouse embryos (QIAamp DNA mini kit, 51304 or AllPrep DNA/RNA Micro Kit, Qiagen, 82084) and spike-in lambda DNA (1.25 ng ...
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bioRxiv - Systems Biology 2019Quote: ... rRNA depletion of the poly(A)-tailed RNA isolated from yeast cells and mouse tissues was subsequently performed using a GeneRead rRNA Depletion kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Briefly, mouse ESCs treated with non-targeting control siRNA (Dharmacon, D-001810-02-50)or NF-YA siRNA (Qiagen, SI01327193) were collected 48 hours post-transfection in cold PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen right hemibrain was crunched in liquid nitrogen and 10-20mg of pulverized mouse brain was used for RNA extraction with RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality were determined using Agilent RNA 6000 Nano Kit according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... 4 – 4.5 week-old neurons co-cultured with mouse astrocytes were lysed and total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... were premixed and added to each well of RT² Profiler™ PCR Array Mouse NFκB Signaling Pathway (Qiagen, PAMM-025Z) for target gene qPCR ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative RT-PCR analysis of the samples was carried out using the mouse antiviral response RT2 Profiler PCR array (Qiagen) on a 7900HT Fast Real-Time PCR system (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... gDNA was isolated from at oncogene-negative mouse cell lines or FACS-sorted Tomatopositive cancer cells using either the AllPrep DNA/RNA(Qiagen) or the DNeasy Blood and Tissue Kit ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplicons of exon 5 of the mouse Nprl3 gene were purified using a PCR purifications Kit (Qiagen, Hilden, Germany) and sent to the Massachusetts General Hospital Center for Computational and Integrative Biology DNA Core for whole amplicon next-generation sequencing (NGS ...
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bioRxiv - Genomics 2021Quote: ... For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Bacterial DNA was extracted from sorted and unsorted mouse fecal-derived bacterial cells using the AllPrep PowerFecal DNA/RNA kit (Qiagen) as per manufacturer instructions ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: Total RNA from WT and ΔIg3-MuSK mouse soleus and TA was extracted using a RNeasy Fibrous Tissue mini kit (Qiagen) for non-stranded RNA library preparation and sequencing (Genewiz).
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bioRxiv - Immunology 2022Quote: ... Three hundred cells from three to six mouse per condition were sorted directly into 25 μl TCL buffer (Qiagen, 1031576) supplemented with 1%β-mercaptoethanol at single cell precision using a BD FACS Aria II instrument ...
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bioRxiv - Immunology 2022Quote: ... For this analysis bacterial DNA was isolated either from feces or from 1 cm of mouse terminal ileum using QIAmp Fast DNA Stool kit (QIAGEN). qPCR was performed using SYBR Green I qPCR Master mix on LC480II Light cycler (Both Roche) ...