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Citations for Qiagen :
1051 - 1100 of 1336 citations for Lassa Fever Virus GP2 Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... DNA and RNA was extracted from fresh-frozen tissue samples using the AllPrep DNA/RNA/Protein minikit (Qiagen, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant SlERF.D7-6XHIS protein was purified by Ni2+gravity flow chromatography according to the manufactureer’s protocol (Ni-NTA Agarose, Qiagen) as described ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant RIN-6XHIS protein was purified by Ni2+ gravity flow chromatography according to the manufacturer’s protocol (Ni-NTA Agarose, Qiagen). For the EMSA assay ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from FACS-purified astrocytes from a single mouse brain using the QIAshredder and the All-prep DNA/RNA/protein Mini Kit according to the manufacturer’s protocol (Qiagen). Total RNA concentration was measured using a NanoVue PlusTM spectrophotometer (Biochrom) ...
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bioRxiv - Biochemistry 2022Quote: ... Culture medium was harvested 72 h post-transfection and secreted His-tagged proteins purified using Ni-NTA agarose (Qiagen) in batch mode ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant protein was purified by affinity chromatography using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant protein was purified by affinity chromatography using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
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bioRxiv - Microbiology 2024Quote: ... Recombinant proteins were purified to near homogeneity (>95%) using Ni-chelate affinity chromatography on Ni-NTA Superflow resin (Qiagen) using standard protocols ...
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bioRxiv - Genetics 2023Quote: ... Precipitated proteins were resuspended in 6M guanidine hydrochloride and incubated with 25 µL of Ni-NTA agarose beads (Qiagen) overnight ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were analyzed by NTA and then processed with AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen).
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from these samples according to the manufacturer’s instruction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). RNA was quantified using a Nanodrop-1000 spectrophotometer (Thermo-Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from hippocampus and pre-frontal cortex was purified with AllPrep DNA/RNA/Protein Mini Kit (QIAGEN, 80004) and DNAseI digested (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction containing the His-VitR protein was purified using NTA-resin affinity chromatography in phosphate buffer according to manufacturer’s recommendations (Qiagen). After concentration (Vivaspin 6 Concentrator ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was isolated using the SampleIN Direct PCR Kit (highQu) or the AllPrep DNA/RNA/Protein Mini Kit (Qiagen), and amplified with ALLin HS Red Taq Mastermix (highQu ...
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bioRxiv - Developmental Biology 2020Quote: We isolated RNA from single mouse embryonic hearts at embryonic stage E12.5 by using the RNeasy Kit (#74104; Qiagen) and the RNase-Free DNase Set (#79254 ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from snap frozen striatal mouse tissue from 2 independent cohorts of mice according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 13 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from mouse brains with TRIzol or from selected cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted either from mouse total lung tissue after homogenization using the RNeasy Mini Kit (Qiagen GmbH) or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit ...
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bioRxiv - Bioengineering 2020Quote: Genomic DNA from mouse tissues and cultured HEK293 cells were extracted using DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD). Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research ...
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bioRxiv - Neuroscience 2020Quote: RNA from rat Schwann cell cultures or mouse nerve tissue was extracted using an RNeasy Micro Extraction Kit (Qiagen). RNA quality and concentration was determined after extraction using a nanodrop 2000 machine (Thermo) ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was extracted from blood and mouse lung tissues using a Blood and Tissue DNA kit (Qiagen, Germantown, MD). Rat and mouse DNA were quantified by RT-qPCR using rat- and mouse-specific primers included in Supplementary Table 1 (Walker et al ...
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bioRxiv - Immunology 2022Quote: ... The recombinant plasmids for mouse vaccination were prepared using an EndoFree Plasmid Purification Kit (Cat# 12391, Qiagen, Hilden, USA).
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bioRxiv - Molecular Biology 2021Quote: ... and genomic DNA from MEFs and mouse tissue samples were extracted using the DNeasy Blood and Tissue Kit (Qiagen), according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from cultured cells or mouse kidneys using the total RNA extraction miRNeasy mini kits (Qiagen). cDNA synthesis was performed using the iScript cDNa synthesis kit (Bio-rad) ...
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bioRxiv - Microbiology 2020Quote: RNA from hNE apical washes and mouse oropharyngeal swabs were prepared using QIAamp® Viral RNA Mini Kit (QIAGEN) and Nucleospin® RNA kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was isolated from E11.5 wildtype and humanized mouse dorsolateral telencephalon using the RNAeasy Micro Plus Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Mouse lungs were isolated and snap-frozen in liquid nitrogen or suspended in Allprotect Tissue Reagent (Qiagen, Hilden, Germany). RNA was extracted as directed using the Qiagen RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was generated from 0.1 grams of feces from each mouse using the power soil DNA kit (QIAGEN) according to the manufacturer’s instructions except that cells were broken by a bead beater instead of a vortex ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was isolated from pooled E12.5 CD1 mouse embryos using an RNeasy® Plus Mini Kit (Qiagen, Hilden, Germany) and then used to generate cDNA with a QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Neuroscience 2019Quote: ... and loaded into a RT² Profiler™ PCR Array Mouse Signal Transduction Pathway Finder™ (cat# PAMM-014Z, Qiagen). These plates were then run on an Applied Biosystems model StepOne Plus PCR machine.
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bioRxiv - Physiology 2021Quote: Mouse fetal hearts were individually homogenised using a stainless steel bead in 500μl RLT buffer (RNeasy, Qiagen, Manchester, UK) and 1% β-mercaptoethanol with a TissueLyser II (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from a pool of 500 MN per mouse with the microRNeasy kit according to manufacturer’s instruction (Qiagen). RNA was quantified with a Qubit (fluorimeter from Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and regulatory network construction was performed for each mouse model using Ingenuity Pathway Analysis (IPA, QIAGEN Bioinformatics, version 52912811). Fold-change values for each protein in the PSD proteome was used as input for a Core Analysis in IPA using the following default settings ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA was extracted from human and mouse feces-derived bacterial supernatant using the DNeasy powersoil kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA was extracted from the midbrain of each mouse using the RNeasy® Plus Universal Mini Kit (Qiagen, Germany). The reverse transcription was performed using 300 ng of total RNA mixed with 3.8 μM of oligo(dT)20 (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... and RT2 Profiler PCR Array Mouse WNT Signaling Pathway ABI 7900HT Standard Block plates (Qiagen; Cat. No. PAMM-043ZA) were used for qPCR.
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bioRxiv - Cancer Biology 2024Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Molecular Biology 2024Quote: ... For Southern blotting or qPCR total DNA was isolated from mouse tissues using the DNeasy Blood & Tissue Kit (QIAGEN) as previously described(Kühl et al. ...
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bioRxiv - Microbiology 2024Quote: Bacterial DNA was extracted from sorted cells and mouse fecal pellets using the AllPrep PowerFecal DNA/RNA kit (Qiagen) as previously described.13 Extracted DNA underwent library preparation and 16S rRNA gene sequencing of the V4-V5 hypervariable region with 515F/926R primers using the Illumina MiSeq PE250 system at the Université de Québec à Montréal (UQAM ...
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bioRxiv - Pathology 2023Quote: Total RNA was isolated from mouse liver tissues according to the manufacturer’s instructions (RNeasy® Mini Kit, 74106, Qiagen). RNA samples were incubated with DNase I (79254 ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was isolated from mouse fecal samples following the standard protocol from the DNeasy PowerSoil Kit (Qiagen). Sequencing libraries were generated according to methods adapted from Caporaso et al ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen; 2x 2 min cycles at 30 Hz) and 5 mm stainless steel beads ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA from the mouse brain and spleen was isolated with the QIAmp DNA Mini Kit (Qiagen, Cat# 51304), mRNA from the plasma was isolated using the QIAamp MinElute Virus Spin Kit (Qiagen ...
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bioRxiv - Biophysics 2021Quote: The nsp10:16 protein complex was supplemented by 1 mM sinefungin Crystals of the nsp10:nsp16 protein complex grew in three days in commercial JCSG I-IV screens (Qiagen). They were cryo-protected in mother liquor supplemented with 20% glycerol and flash frozen in liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the two assay plasmids by temperature-cycle ligation.
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bioRxiv - Cell Biology 2022Quote: ... were performed on significantly altered proteins in CAVIN1-null cells using the “core analysis” function included in the Ingenuity Pathway Analysis (IPA) software (QIAGEN Bioinformatics ...
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bioRxiv - Molecular Biology 2020Quote: RNA and protein were extracted from cell lines using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA, USA). Total RNA was also DNase treated using the TURBO DNA-free Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: RNA was extracted simultaneously from the same tissue pieces used for DNA extraction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...