Labshake search
Citations for Qiagen :
1101 - 1150 of 3581 citations for 6 Methyl 3 4 dihydro 2H pyrido 3 2 b 1 4 oxazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Enzymatic lysis and proteinase K digestion of bacteria was performed following protocol 4 in the RNAprotect® Bacteria Reagent Handbook (Qiagen, Germany). Purification of total RNA from bacterial lysate using the RNeasy® Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... In replicates 1 and 2 a Universal extraction robot (Qiagen, Germany) and associated nucleic acid extraction (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from frozen Hepa 1-6 cell pellets using the RNeasy Mini kit (74106, QIAGEN) following the manufacturer’s instructions with an on-column DNase digestion step (79254 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A2 and B Wolbachia using PyroMark Assay Design 2.0 (Qiagen, USA). A complete list of primers sequences could be found in Table S3 ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using a Puregene Tissue Core Kit B (Qiagen).
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bioRxiv - Genetics 2019Quote: ... expand them and extract genomic DNA (Puregene Core Kit B, Qiagen) to improve the quality of the sequencing reaction ...
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bioRxiv - Physiology 2019Quote: ... to remove the cell debris and were stored at −80°C for no longer than 4 weeks until isolation of cf DNA with QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany) and measurement of plasma concentrations of cf n-DNA and cf m-DNA with real time quantitative PCR as described previously3 ...
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bioRxiv - Developmental Biology 2019Quote: The Duke Microbiome Shared Resource (MSR) extracted bacterial DNA from gut and water samples using a MagAttract PowerSoil DNA EP Kit (Qiagen, 27100-4-EP) as described previously (Murdoch et al. ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA (gDNA) extraction was performed using a custom liquid handling protocol based on the Qiagen MagAttract PowerMicrobiome DNA/RNA Kit (Qiagen 27500-4-EP) adapted for lower volumes ...
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bioRxiv - Systems Biology 2020Quote: Total RNA was extracted from hypothalamus and hippocampus (n = 4 per dietary group per tissue; total 32 samples) using an All-Prep DNA/RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Sample size was based on previous RNA-Seq studies in which findings were validated using qPCR and gene perturbation experiments [6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA from 4-8 microdissected 10 μm FFPE sections were extracted using the QIAamp DNA FFPE Tissue kit (Qiagen, cat. no. 56404) according to manufacturer instructions ...
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Assessment of Human Renal Transporter Based Drug-Drug Interactions Using Proximal Tubule Kidney-ChipbioRxiv - Cell Biology 2022Quote: Total RNA was isolated from cells in the Kidney-Chip (4 individual samples obtained on Day 14) and transwells (4 samples obtained on Day 14) using buffer RLT plus and RNeasy® Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... The supernatant was transferred to clean 1.5mL MCF tube and to which 350 μL ethanol at 4’C was added before vortexing for 10 sec and transferring the supernatant to a QIAamp Mini spin column (Qiagen Germantown, MD U.S.A.). The sample was process as recommended in the QIAamp DNA Mini Handbook and eluted with 10 mM Tris-Cl ...
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bioRxiv - Microbiology 2021Quote: Total genomic bacterial DNA extraction was performed from frozen fecal samples using the Qiagen DNeasy PowerSoil HTP Kit (Cat. No. 12955-4, Qiagen, Valencia, CA, USA). Adequate DNA yield was confirmed using NanoDrop spectrophotometry ...
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bioRxiv - Microbiology 2022Quote: ... exposed to 0.4 nM and 4 nM of β-estradiol for 10 days) Vagina Chips using QIAzol lysis reagent (Qiagen, cat. no. 79306). Complimentary DNA was synthesized using a SuperScript VILO MasterMix (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The soluble fraction was separated from debris by centrifugation (9000 g, 4°C) and incubated for 60 minutes with Ni-NTA beads (Qiagen, cat. no. 30230) pre-equilibrated in binding buffer ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from the hippocampus and cortical regions of Ctrl WT and cKO mice (n=4 per group) using the RNeasy kit (Qiagen, Valencia, CA, USA) method (see location of cortical areas 1 and 2 in the Supplementary Figure 1) ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from dolphin oral samples and 32 negative (PBS) controls using the DNeasy UltraClean 96 Microbial Kit (Qiagen Cat. #10196-4) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... for 30 min at 4 °C before being applied to a 10mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... were homogenized in 2-mL tubes containing lysis buffer plus 1% 2-mercaptoethanol using a TissueLyser LT bead mill (Qiagen) and 5-mm stainless-steel beads (Qiagen #69989 ...
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bioRxiv - Microbiology 2022Quote: ... and 150 μL DMEM supplemented with 2% FBS and homogenized using a TissueLyser II (2 cycles at 30 Hz, 1 min, Qiagen). Homogenates were clarified by centrifugation and frozen until plaque assay titration ...
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bioRxiv - Developmental Biology 2021Quote: ... single-end BS-seq libraries were also constructed with Ovation Ultralow Methyl-Seq Library Systems (Nugen, 0336) and EpiTect Bisulfite Kit (Qiagen, 59104) kits according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Further confirmation of which allele was deleted in clones with only one UBE3A copy was performed by utilizing the EpiTect Methyl II DNA Restriction Kit (QIAGEN, #335452) to measure methylation at the PWS-IC (SNRPN ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected with 1:1:2 μg of packaging plasmids versus shRNA hairpins on the pLKO.1 vector using Effectene transfection reagent (Qiagen) 48 h prior to harvesting supernatants ...
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bioRxiv - Cancer Biology 2021Quote: Liver tissue was homogenized in chloroform/methanol (2:1, 1 mL) using TissueLyser (Qiagen Ltd., Manchester, UK). Deionized water (400 uL ...
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bioRxiv - Plant Biology 2021Quote: ... nks1-1 and nks1-2 using RNeasy Plant mini kit (74904, QIAGEN). cDNA was synthesized from 1 μg total RNA using iScript cDNA synthesis kit (1706691 ...
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bioRxiv - Genetics 2020Quote: ... rad54-1 and rad54-2 plants using RNeasy Plant mini Kit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The samples then had a 2:1 ratio of RNAprotect (Qiagen: #76506) added to them and centrifuged at 5,000 RCF for 10 min ...
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bioRxiv - Biophysics 2022Quote: ... combined with Ni-NTA resin (2 mL/1 L of biomass, Qiagen) pre-equilibrated with 40 mM sodium phosphate buffer ...
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bioRxiv - Microbiology 2023Quote: ... each treated culture was mixed 1:2 with RNA Protect (Qiagen, Germany), vortexed for 10 seconds ...
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bioRxiv - Microbiology 2024Quote: ... 2 volumes (1 mL) of RNA protection bacteria reagent (Qiagen, Hilden, Germany) were added ...
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bioRxiv - Cancer Biology 2019Quote: ... Germany) using a metal ball for 2×2 min at 25 s−1 in 600 μl of RLT buffer (Qiagen, Germany) with 1% β-mercaptoethanol ...
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bioRxiv - Genetics 2024Quote: The organs of Corti of postnatal day (P)4 mice were dissected and stored at -20°C in RNAprotect Tissue Reagent (RNAlater®) (QIAGEN, cat. no. 76106). Total RNA was extracted using the SPLIT RNA extraction kit (Lexogen ...
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bioRxiv - Genetics 2022Quote: ... biological diseases and functions as well as the FOXP1/4 upstream transcriptional network were identified using QIAGEN’s Ingenuity Pathway Analysis software (IPA®, QIAGEN Redwood City, www.qiagen.com/ingenuity). Heatmaps with hierarchical clustering were constructed using the heatmap.2 package in R ...
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bioRxiv - Microbiology 2023Quote: ... 3–20 μl of individual PCR products (adjusted on the basis of estimated relative amplicon concentration) were combined into 100 μl subpools and purified using an UltraClean 96 PCR cleanup kit (Qiagen Beverly LLC #12596-4). Despite not producing visible PCR bands ...
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bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... The SCGs cultured in 6-well dishes and one entire 6-well was harvested and pooled together for RNA isolation (RNeasy, Qiagen) per sample ...
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bioRxiv - Neuroscience 2024Quote: ... 6 µl Hi-Perfect transfection reagent (Qiagen, 301707), and 1 µl of the desired (20 µM ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Cell Biology 2019Quote: ... 1.2×106 cells were plated into each well of a 6-well plate and transfected with Sns and Eff-1 constructs (200ng each) using Effectene (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Genetics 2019Quote: ... for the PCR analysis of potential recombination events genomic DNA from 1×10^6 cells was extracted using DNeasy Blood & Tissue Kits (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 1× PBS and total DNA was extracted at 6 hpi with DNeasy Blood/Tissue DNA mini kit (Qiagen) and quantitated by a nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 30-70 mg of tissue or 5.0 x 10^6 cells for Source 1 cell lines using commercially available QIAGEN QIAamp Mini Kit (QIAGEN, 51304), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice with 1× PBS and total DNA was extracted at 6 hpi with a DNeasy Blood/Tissue DNA mini kit (Qiagen) and total DNA yield was measured by a nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mL of stabilization mix (RNAprotect Bacteria Reagent - Qiagen diluted with PBS in a 2:1 volume ratio) was applied on plates ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were centrifuged at 16,000 g for 5 min and supernatant was treated with 4 µL of RNase A (100 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19101), gently mixed by flicking of tube and incubated at room temperature for 2 min ...
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bioRxiv - Genomics 2022Quote: An aliquot of 150 μL of plasma per sample was thawed on ice and centrifuged at 3000 × g for 5 min at 4 °C and smallRNA was extracted using miRNeasy Serum/Plasma Kits (Qiagen, Cat. No. 217184, Milano, Italy) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... with 1% 2-Mercaptoethanol (Serva) and disrupted using the Qiagen Tissue Ruptor (Qiagen). Total RNA was extracted using the RNeasy Fibrous Tissue Mini Kit (Qiagen ...