Labshake search
Citations for Qiagen :
1101 - 1150 of 2825 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... with the addition of a 2-minute spin at 15,000 x g in the QIAshredder (Qiagen, Germantown, MD) prior to the genomic DNA elimination step ...
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bioRxiv - Genomics 2024Quote: ... Amplicons were generated in 2 different 25 µL PCR reactions (9.5 µL nuclease-free water, 12.5 µL 2x QIAGEN Multiplex PCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 µg of gDNA was used for bisulfite conversion using the Epitect bisulfite conversion kit (QIAGEN 59124) or 200 ng gDNA was used for enzymatic conversion (NEB E7125) ...
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bioRxiv - Developmental Biology 2024Quote: ... 200 ng psiCHECK-2 reporter plasmid were co-transfected with 5 µl miScript miRNA mimics (Qiagen; CatNo. 219600) using 4 µl Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... containing 2-mercaptoethanol (1µL/100µL RLT) and total RNA was isolated using the RNeasy Plus Micro Kit (Qiagen). RNA concentration was determined with the NanopDrop One spectrophotometer (NanoDrop ...
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bioRxiv - Biophysics 2024Quote: ... for 4 hours at 37°C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). At this stage ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was the incubated at 4 °C with 2 ml of washed Ni-NTA agarose beads (Qiagen) for 1 hour ...
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bioRxiv - Genomics 2020Quote: ... 1 ul (1U/ul) UDG and 1 ul RNaseA (100 mg/ml, Qiagen) and reactions were incubated at 37°C for 3 hrs ...
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bioRxiv - Genomics 2023Quote: ... gracilis (1 male and 1 female) using the Rneasy Mini Plant Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The solubilized fraction was then incubated for 2 h with 300 µL of pre-equilibrated Ni-NTA resin (QIAGEN) in Ni-NTA buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: cfDNA was isolated from 2 mL of plasma for each sample via the QIAamp Circulating Nucleic Acid kit (Qiagen). ddPCR was performed using the QX200 Droplet Digital PCR System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... the second PCR used 2 µL of the first reaction as template and HotStarTaq DNA Polymerase (Qiagen, Hilden, Germany) in a 20 µL reaction volume for 15 cycles ...
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bioRxiv - Immunology 2021Quote: ... RNA of cells exposed to SARS-CoV-2 WT was isolated with the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: DNA was extracted from the exterior (outermost 2 mm) tissue and water filters using the DNeasy PowerSoil Kit (Qiagen) and from the interior tissue (at least 2 mm away from the outermost edge of the specimen ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from snap frozen striatal mouse tissue from 2 independent cohorts of mice according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 13 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Immunology 2022Quote: ... in vitro generated PCs were sorted at day 2 and RNA were extracted with “RNeasy plus micro kit” (Qiagen) as recommended ...
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bioRxiv - Genomics 2020Quote: ... and RNA was eluted using DTT (100mM, 2 × 100μL) and collected into 700μL RLT buffer from the RNeasy MinElute Cleanup Kit (Qiagen 74204). RNA was isolated using the RNeasy MinElute kit as per manufacturer’s instructions and was quantified using the Agilent Tapestation High Sensitivity RNA kit (Agilent 067-5576) ...
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bioRxiv - Neuroscience 2020Quote: ... each individual cell was transferred to a 0.2 ml PCR tube containing 2 μl of lysis buffer (RLT Plus - Qiagen). The tube was immediately placed on a metal plate sitting on top of dry ice for flash-freezing ...
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bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...
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bioRxiv - Immunology 2021Quote: ... was then added to a polymerase chain reaction (PCR) mix containing 12.5 μl of 2× Multiplex PCR mix (Qiagen), 9 μl of H2O ...
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bioRxiv - Cell Biology 2021Quote: Meta-analysis of the DESeq2 analysed RNA sequencing data was performed to identify the top pathways that are statistically associated with the list of the significantly induced genes77 (fold change > 2, FDR q value < 0.05, using the Ingenuity Pathway Analysis platform IPA, Ingenuity Systems, Qiagen).
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bioRxiv - Cell Biology 2021Quote: Total RNA extracts were prepared from a minimum of 2×104 freshly sorted cells using RNeasy Micro Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of microRNA was reverse transcribed using miScript Ⅱ microRNA cDNA Synthesis Kit (QIAGEN Biotechnology Co, Ltd, China). The reverse transcription reaction system included 4 μL of 5×miScript HiFlex Buffer ...
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bioRxiv - Microbiology 2021Quote: The QIAseq SARS-CoV-2 Primer Panel paired with the QIAseq FX DNA Library construction kits (Qiagen GmbH, Germany) were used for enriching and sequencing the entire SARS-CoV-2 viral genome ...
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bioRxiv - Molecular Biology 2020Quote: ... was added to the embryos and homogenized for 2 min at 40 Hz using a Tissue Lyser LT (Qiagen) and 5 mm stainless steel beads (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from supernatants of SARS-CoV-2-infected Vero E6 cells using the RNeasy kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 2 g powdered clay samples using the PowerMax Soil DNA Isolation Kit (Qiagen, Germany) and modifications to the manufacturer’s instructions previously validated (22) ...
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bioRxiv - Microbiology 2022Quote: ... One volume of bacterial culture containing approximately 0.2 OD600 of cells was mixed with 2 volumes of RNAprotect (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Purified ZIKV RNA and HSV-2 DNA were extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704), and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of cDNA was then added to a PCR mix containing 12.5 μl 2× Multiplex PCR mix (Qiagen), 9 μl H2O ...
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bioRxiv - Biochemistry 2020Quote: ... Nanobody PCR fragments were analyzed on 2% TAE-agarose gels and purified with the QIAquick gel extraction kit (Qiagen). PCR fragments were sequenced and individual nanobodies were cloned using the In-Fusion cloning kit (Takara Bio ...
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bioRxiv - Cancer Biology 2021Quote: ... The products were run on 2% agarose gel and extracted using the QIAquick Gel Extraction Kit (Qiagen, Cat#28706). The purified products were submitted for paired-end 300 bp sequencing on MiSeq (Illumina) ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were transfected with a mixture of 2 mg SPACA6 pMT-puro vector and Effectene transfection reagent (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... PCR amplicons were size selected on a 2% agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen), and sequenced and deconvoluted using the Amplicon-EZ service provided by GENEWIZ/AZENTA (South Plainfield ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were isolated from a 2% agarose gel using the QiaQuick gel extraction kit (Qiagen, Cat. No. 28706) and their quality and concentration were determined using a 2200 TapeStation and D1000 screen tapes (Agilent Technologies) ...
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bioRxiv - Systems Biology 2021Quote: RNA isolation from SARS-CoV and SARS-CoV-2 infected A549-ACE2 cells was performed as described above (Qiagen). 500 ng total RNA was used for reverse transcription with PrimeScript RT with gDNA eraser (Takara) ...
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bioRxiv - Physiology 2021Quote: ... and homogenized with metal beads (2.4 mm diameter, Askubal) for 2 min at 25 Hz in a TissueLyzerII (Qiagen). Beetles were weighed and frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein was normalized to 2 mg across all samples tested and incubated with 0.25 mL of 50% Ni-NTA resin (Qiagen) for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 g of meat or processed food powder was extracted and purified using DNeasy Mericon Food Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Libraries were constructed using QIASEQ SARS-CoV-2 Primer Panel and QIAseq FX DNA Library UDI-A kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Tissue lysates were prepared from 30 pooled 2 dpf WT and MZdnmt3aa-/- mutant by homogenization using TissueLyser LT (QIAGEN) with zirconia beads for 10 s at 50 Hz ...
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were harvested after 2 hours of treatment and RNA was isolated with the RNeasy Mini Kit (Qiagen #74104). RNA levels were assessed using qScript™ XLT One-Step RT-qPCR ToughMix® (Quanta Bioscience #95132-100 ...
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bioRxiv - Bioengineering 2021Quote: ... The filtered supernatant was incubated for 2 hours at room temperature with 6ml of Ni-NTA resin (Qiagen, 30210). The column containing the mix of supernatant and resin was washed four times with a washing buffer containing 20mM imidazole ...
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bioRxiv - Genomics 2020Quote: ... PCR based COVID-19 viral genome enrichment was performed using QIAseq SARS-CoV-2 Primer Panel Kit (Qiagen, Germany). A total of 98 primer pairs specific to COVID-19 were used for enrichment where primers pairs were distributed in two pools (Pool-1 and Pool-2) ...
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bioRxiv - Molecular Biology 2021Quote: The AR9 gene 226 was PCR amplified from AR9 genomic DNA and cloned into the pQE-2 vector (QIAGEN) between the SacI and SalI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 mL of samples were collected and spun at 12,000 rpm for 2 minutes and resuspended in 350 µL RNA Protect Bacterial Reagent (Qiagen). Samples were kept on ice from this point forward ...
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bioRxiv - Immunology 2023Quote: ... PCR products were run on a 2% agarose gel and purified using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). The obtained TCRβ libraries were quantified using the PerfeCTa-NGS-Quantification Kit according to the manufacturer’s protocol (Quantabio Inc ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of logarithmic growth phase culture was pelleted by centrifugation and resuspended by vortexing in B1 buffer (Qiagen, Hilden Germany ...
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bioRxiv - Immunology 2022Quote: ... The separated PBMCs were washed with 2% FBS and sample’s mRNA was extracted using RNeasy Plus Mini Kit (Qiagen).