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Citations for Qiagen :
1001 - 1050 of 2825 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... KCl (to a final concentration of 0.3 M) and 2 ml of Ni-NTA beads (Qiagen) were added and incubated at 40 °C for 1.5 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 2% Triton-X 100) and lysed with 200 μL of AL lysis buffer (Qiagen, 19075). DNA was precipitated by adding 1 mL of 100% ethanol supplemented with 100 mM of sodium acetate ...
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bioRxiv - Cell Biology 2021Quote: ... DNase 1 (Qiagen) was used to remove contaminating DNA followed by a second cleanup ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
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bioRxiv - Biophysics 2020Quote: ... or DNAse (200 Units well−1, 1 hr, 37°C, Qiagen). DAPI was stained at 0.5 μM (5 mins ...
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bioRxiv - Immunology 2022Quote: ... Virus was inactivated by mixing 1:1 with Buffer ATL (Qiagen) prior to viral RNA extraction from NP swabs ...
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bioRxiv - Cell Biology 2020Quote: ... His6-SUMO-WASHC2C-5-SNAP was purified using lysis buffer #2 and Ni-NTA-agarose beads (Qiagen). The protein was incubated with Ni-NTA beads and washed with 50 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in sphere-forming cultures for 2 weeks prior to RNA collection (RNAeasy Kit, Qiagen). TGFB1 was replenished when placing mOSE cells in sphere-forming conditions.
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bioRxiv - Molecular Biology 2022Quote: ... Each reaction of the HRM assay included 6.25µl of Type-IT 2 x HRM buffer (QIAGEN, Germany), primers specific for the uidA E.coli housekeeping gene and molecular grade water was added to make a final volume of 12.5µl ...
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bioRxiv - Microbiology 2022Quote: ... 2×109 bacteria were pelleted and DNA was extracted using a DNeasy Blood and Tissue kit (Qiagen). The optional RNase step in the protocol was performed to remove contaminating RNA ...
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted twice using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting amplicon pool was further purified from a 2% agarose gel (QIAquick Gel extraction Kit Qiagen) with GelRed staining and eluted in TE (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was incubated for ~2 h rotating at 4°C with Ni-NTA Resin (Qiagen, 1018244) that had been washed with wash buffer (20mM Tris HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: 2 x 106 cells were harvested and RNA was purified using Qiagen RNeasy Mini Kit (Qiagen, #74104) using manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell supernatant was extracted using the QIAamp viral RNA mini kit (Qiagen). A two-step qRT-PCR was used to detect viral RNA released in the cell supernatant ...
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bioRxiv - Developmental Biology 2022Quote: ... and 2 μg of extracted total RNA was reverse-transcribed using the Omniscript RT Kit (205111, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 S mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Genetics 2021Quote: Total RNA was prepared from AKR and DBA/2 BMDM using miRNeasy Mini Kit (Qiagen; # ID 217004) with on-column DNA digestion according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... Samples were homogenized at 30 Hz for 2 min using a TissueLyser II (QIAGEN GmbH, Hilden, Germany) and centrifuged for 30 sec at 11,000 rpm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and homogenized twice at 10 s at 2 M/s with a 5-mm steal bead (Qiagen) using a tissue homogenizer (MP Biomedicals) ...
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bioRxiv - Genetics 2020Quote: ... RNases were removed by incubating samples in the presence of 2 ul of RNase A (Qiagen, 158922) for 30 min at 37°C and samples were chilled for 5 on ice ...
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bioRxiv - Microbiology 2021Quote: Enrichment for viral RNA was performed using the QIASeq™ SARS-CoV-2 Primer Panel V2 (Qiagen) Panel V2 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 replication was quantified via RT-qPCR using the QuantiTect Multiplex RT-qPCR Kit (Qiagen) with a Rotor Gene Q cycler (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was transferred to fresh tubes and incubated with 2 μl RNase A (Qiagen, 20 mg/ml) and 6 μl 5M NaCL (Active Motif ...
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bioRxiv - Genomics 2021Quote: ... in a 20 μL system with a mixture of 10 μL 2×SYBR Premix ExTaq (Qiagen, Germany), 2.0 μL diluted cDNA or double distilled water as a negative control ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 S mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... Products between 200bp-600bp were purified from 2% agarose gels run in 0.5XTAE using QIAquick kits (Qiagen), and excess adaptors were removed by magnetic beads (HighPrep PCR ...
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bioRxiv - Synthetic Biology 2022Quote: ... first the filter-harvested cells were resuspended in 2 mL RNAprotect Bacterial Reagent (Qiagen Catalog no. 76506), then pelleted in a centrifuge ...
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bioRxiv - Immunology 2022Quote: ... the frozen lung specimens were weighed before homogenization in PBS/2% FBS solution using the TissueLyser (Qiagen), as previously described (Tseng et al. ...
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bioRxiv - Genomics 2022Quote: ... Samples were incubated overnight at 55°C before adding 2 µl of RNaseA (100 mg/ml, Qiagen) and incubated for 15 min at 45°C ...
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bioRxiv - Neuroscience 2023Quote: Total mRNA was extracted from the PBMC obtained from 2 alpacas using standard RNA extraction kit (Qiagen). Recovered mRNA was retrotranscribed to cDNA by using Supercript IV (ThermoFischer ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was then performed in a 20 μL reaction volume with 2×SYBR Green qPCR mix (Qiagen), 100 nM each of the sense and antisense primers (Table 1) ...
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bioRxiv - Genomics 2022Quote: ... cfDNA was extracted from 2 to 4 ml of plasma using the QIAsymphony liquid handling robot (Qiagen). cfDNA concentration was determined using Qubit double-strand molecular probes kit (Invitrogen ...
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bioRxiv - Pathology 2022Quote: ... HSP90AB1) and fold changes/regulation of gene expression were calculated using the 2^(−ΔΔCT) formula (GeneGlobe, QIAGEN). Differential expression (up and down regulation ...
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bioRxiv - Cell Biology 2022Quote: Amplicons were separated on 2% agarose-TAE gels and purified using the QIAquick Gel Extraction Kit (Qiagen). Amplicons were sequenced at Quintarabio ...
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bioRxiv - Microbiology 2024Quote: ... 2 g of soil were used for RNA extraction using the RNeasy PowerSoil Kit (Qiagen, Venlo, Netherlands) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... 2 or 4 days and genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen). Bisulfite converted DNA libraries were prepared using the Accel-NGS Methyl-Seq DNA library kit (SWIFT BIOSCIENCES) ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from 2-5 million flash frozen cells using the RNeasy plus mini kit (Qiagen). Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Primers for Jamaican fruit bat genes (Supplemental Table 2) and qPCR was performed (QuantiTech SYBR Green, Qiagen). Within sample gene normalization was performed on Rps18 (ΔCq) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cotyledons from 100 plants were dissected and ground in 2 ml tubes using a Tissue Lyser (Qiagen) for 1 min 30 sec at 30 Hz before RNA extraction using the RNeasy micro kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris pH 8.5, 2 mM MgCl2, 0.5% NP40, 0.5% Tween20, 0.05 U/mL QIAGEN protease) and following the procedure described in (42) ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen) in a gravity flow column and then washed with 300 mL of lysis buffer D ...
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bioRxiv - Biochemistry 2022Quote: IQN17 was crystallized at room temperature in a hanging-drop vapor diffusion system by mixing 0.3 μL of 15 mg/mL peptide with 0.3 μL of reservoir solution containing 0.1 M imidazole (pH 8.0) and 35 % 2-methyl-2,4-pentanediol (MPD) (Qiagen). Crystals were seen two days after trays were set up ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from 2 biological replicates of fecal pellets using DNeasy PowerLyzer PowerSoil kit (Qiagen). Concentration of Pc and Bt DNA was assessed using species-specific qPCR primers (Key Resources Table) ...
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bioRxiv - Biophysics 2023Quote: ... The filtered supernatant was loaded onto 2 gravity columns each with 4 mL Ni-NTA agarose (Qiagen). The resin was pre-equilibrated with the lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was collected and incubated with 2 mL of Ni-NTA Superflow affinity resin (Qiagen), previously equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted at 2 or 8 hpi with DNeasy Blood/Tissue DNA mini kit (Qiagen) and quantitated by a nanodrop spectrophotometer ...
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bioRxiv - Genetics 2024Quote: ... the final pool was purified from a 2% agarose gel (QIAEX II Gel Extraction Kit, Qiagen #20021).
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bioRxiv - Plant Biology 2024Quote: ... Each extraction step was carried out for 2 h in a TissueLyser II (Qiagen AB, Sollentuna, Sweden) at 6 s-1 at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and centrifuged (2 minutes 12,000 RPM) before DNA-containing supernatant (2µL) was added to PCR master mix (18µL, Qiagen 201203 ...