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Citations for Qiagen :
1051 - 1100 of 2036 citations for Polystyrene Latex Beads 5 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... After mechanical disruption by shaking at 25/s over 4 min with metal beads (TissueLyser II, Qiagen), two 250 μL aliquots of the homogenate were stored at -80°C for viral plaque titration ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 and 0.1 μg) were allowed to bind 30 μl of nickel-nitrilotriacetic acid-agarose beads (QIAGEN) for 1 hour after which unbound decoy was removed by washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected in a fresh tube and further incubated with Ni- NTA resin beads (Qiagen) for 2 hr at 4°C with 5 rpm rotation ...
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bioRxiv - Genetics 2024Quote: ... The eluate was separated from the beads and RNA was purified using RNeasy Plus Mini Kit (QIAGEN) according to manufacturer’s protocol.
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bioRxiv - Plant Biology 2024Quote: ... eight discs from each family were homogenized with beads in a TissueLyser® disruptor (Qiagen, Hilden, Germany) to perform DNA extractions using the CTAB protocol (Doyle ...
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bioRxiv - Microbiology 2024Quote: ... and 0.2 g of the sediment slurry was bead-beaten on a TissueLyser LT (Qiagen, Venlo, Netherlands) for 10 min at 50 Hz in a PowerBead tube from the DNeasy PowerSoil DNA isolation kit (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... The DNA was finally purified using 30 µl of silica beads (QIAEX II Gel Extraction Kit, Qiagen), washed and eluted in 22 µl of elution buffer ...
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bioRxiv - Plant Biology 2024Quote: ... and seedlings (tissue type) were crushed with three zirconia beads using the TissueLyser II (QIAGEN, MD, USA) with pre-chilled adapters at -80 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The His-fused ZmbZIP75 was expressed in Transetta (DE3) cells and purified with Ni-HA beads (QIAGEN). The recombinant protein was incubated with biotin-labeled probe ...
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bioRxiv - Genetics 2021Quote: ... Biotinylated RNA was released from beads using 100 mM DTT and cleaned using RNeasy MinElute Kit (QIAGEN 74204). Half of eluted RNA or 600 ng of depleted RNA was ligated to 5 µM of 50 adenylated ...
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bioRxiv - Cancer Biology 2021Quote: Mouse-derived xenograft HCC tumors were homogenized in RIPA buffer using metal beads and the LT TissueLyser (Qiagen). Samples were sonicated (Branson Sonifier 3310s ultrasound probe ...
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bioRxiv - Developmental Biology 2021Quote: ... all remaining bead-bound EGFP+ nuclei were processed for RNA extraction using the RNeasy Plus Micro kit (Qiagen). Nuclear RNA-seq libraries were constructed with the Stranded RNA-seq Kit with Ribo Erase (Kapa Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... two tubes of each strain were freeze-dried overnight and macerated using a TissueLyzer II bead-beater (Qiagen). Two 2 mm metal beads were placed in each tube ...
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bioRxiv - Microbiology 2022Quote: ... beat-beading tubes were filled 1/3 with beads and soaked overnight in 500 μl buffer RLT (Qiagen). Tubes were spun at full speed and excess buffer removed ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was eluted from the beads with RLT buffer and purified using RNEasy MinElute columns (Qiagen, catalog #74204). RNA was eluted into 10 μL of nuclease free water and analyzed on Agilent 2100 Bioanalyzer using the RNA 6000 Pico kit (Agilent ...
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bioRxiv - Plant Biology 2020Quote: ... Magnetic beads (Dynabeads M-270 Carboxylic Acid) were activated and coated with ad hoc designed LNA probes (Qiagen), harboring an −NH2 group ...
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bioRxiv - Microbiology 2021Quote: ... samples were crushed with a mortar and pestle and further homogenized with glass beads in a TissueLyzer (Qiagen). Total RNA was extracted using phenol/chlorophorm/isoamylalcohol phase-separation followed by a GeneJET RNA Purification Kit (Thermo Scientific™) ...
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bioRxiv - Physiology 2021Quote: Liver homogenates were prepared in cold conditions (+4°C) by adding a stainless-steel bead (Qiagen, cat. 69989) and tissue lysis buffer (composition ...
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bioRxiv - Plant Biology 2021Quote: ... small pieces of leaf tissue were flash frozen in liquid nitrogen and ground in a bead mill (Qiagen). Tissue powder was incubated in extraction buffer (100 mM Tris-HCl pH9.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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bioRxiv - Genomics 2021Quote: DNA from fecal samples was isolated using the MagAttract PowerMicrobiome DNA/RNA kit with glass beads (Qiagen, Germany). Metagenomic libraries were prepared using the NEBNext Ultra II for DNA Library Prep kit (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... 1000 µL PBS was added to each sample (lungs, 0.01–0.04 g) along with Tungsten carbide 3 mm beads (Qiagen). Samples were homogenized at a speed of 10 Hz for 10 min and then centrifuged at 15,000 × g for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 6xHIS-SUMO2 conjugates were purified under denaturing conditions using Ni-NTA-agarose beads according to manufacturer’s instructions (Qiagen). Briefly ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... The soluble fraction was split between two columns each with 2 ml Ni-NTA agarose beads (Qiagen, 30210) pre-equilibrated with Lysis Buffer ...
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bioRxiv - Microbiology 2022Quote: ... the bacterial assemblage genomic DNA was extracted from one g of beads using DNeasy PowerSoil Pro kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were then ground to powder using a 3 mm tungsten carbide bead (Qiagen Cat. No. / ID: 69997) on a TissueLyser II (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... homogenized by cryomilling after addition of a 2.5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 30 seconds 3–6 times ...
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bioRxiv - Microbiology 2021Quote: ... 500 µl of PBS-AB was added to each sample along with Tungsten carbide 3 mm beads (Qiagen). Samples were homogenized during 10 min and then centrifuged at 15,000 g for 10 min ...
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bioRxiv - Systems Biology 2022Quote: ... Supernatants were collected and purified with Ni2+ Sepharose beads (Smart-lifesciences, China) or Ni-NTA Agarose (QIAGEN, Germany), then washed with lysis buffer (For the purification of biotinylated protein ...
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bioRxiv - Plant Biology 2020Quote: ... The clear supernatant was then gently mixed with 1 ml of Ni+2-NTA agarose beads (Qiagen, Germany) for 30 min and then added to the column with a flow rate of 0.5 ml/min ...
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bioRxiv - Developmental Biology 2020Quote: ... benthamiana leaves were grinded in a tube with two glass beads (3 mm) with a TissueLyser II (Qiagen) and directly after supplied with 600 μl EB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The m6A-enriched fragmented RNA was eluted from the beads and purified using the RNeasy mini kit (Qiagen). The quality of the m6A-enriched RNA was assessed with the Bioanalyzer ...
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bioRxiv - Microbiology 2021Quote: ... for 30 minutes at 37°C followed by beadbeating with 50μg 0.1mm zirconium beads for 6 minutes on the Tissuelyzer II (Qiagen) prior to loading onto the Qiacube HT ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 0.6 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture (1 ml beads per 1 l expression culture was used in case of Drosophila BiP ...
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bioRxiv - Microbiology 2020Quote: ... We then washed beads four times in 1 ml of Modified RLT buffer (RLT buffer supplied by Qiagen with added 10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... mother sibling and the index patient using a magnetic bead method and the QIAsymphony automated extraction system (Qiagen). TruSeq PCR Free libraries were prepared for all samples ...
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bioRxiv - Neuroscience 2020Quote: ... lumbar spinal cord or the glabrous skin of the footpad were harvested and homogenized in 0.1M HCl + 0.5% triton X-100 using glass beads and a TissueLyser II (Qiagen). Lysate was centrifuged at 18,000 xg and 4 °C for 40 – 60 min to pellet cell debris ...
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bioRxiv - Cancer Biology 2021Quote: ... Xenograft and HCC mouse samples were homogenized in RIPA buffer using metal beads and the LT tissuelyzer (Qiagen). All samples were sonicated (ultrasound probe ...
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Bioaugmented sand filter columns provide stable removal of pesticide residue from membrane retentatebioRxiv - Microbiology 2020Quote: ... The filters were then transferred to bead tubes supplied with the DNeasy PowerWater Kit (Qiagen, København Ø, DK) and frozen for subsequent DNA extraction according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 500 µL of PBS-AB was added to each sample (Lungs = 0.01 – 0.04g; Cecum = 0.3 -0.5g) along with Tungsten carbide 3mm beads (Qiagen, Gaithersburg, MD). Samples were homogenized at a speed of 10 Hz for 10 min ...
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bioRxiv - Genomics 2020Quote: ... PCR products were cleaned-up using 1:1 of SPRI beads and eluted in 30µl elution buffer (Qiagen). The resulting amplicons were assayed on the Fragment Analyzer System (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was purified using an equal volume of SPRI beads and resuspended in EB buffer (Qiagen, Germany). DNA was quantified with the Quant-iT dsDNA HS kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... which was placed immediately into PowerSoil DNA bead tubes and extracted with the dNeasy PowerSoil Kit (Qiagen, USA) following the standard protocol ...
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bioRxiv - Systems Biology 2023Quote: ... followed by mechanical disruption by glass bead beating and digestion in proteinase K (Qiagen Sciences, Germantown, MD, USA). DNA was extracted and purified using the MagNA Pure nucleic acid purification platform (Roche Diagnostics Corp. ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysate was then filtered through a 0.45 µm syringe filter and mixed with 1.5 mL of washed Ni-NTA Agarose beads (Qiagen). The mixture was allowed to bind under nutation at 4℃ for 2 hr ...
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bioRxiv - Microbiology 2023Quote: ... the resulting supernatant was then applied to 0.1 mm zirconia-silicate beads and mixed with RLT buffer (Qiagen). RNA isolation proceeded as per manufacturer’s instructions using a RNeasy Plus kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA extraction from fecal samples was performed from 200mg of stool transferred to 0.70mm Garnet Bead tubes (Qiagen) with 1.1 mL ASL buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was purified by Ni2+ immobilized metal affinity chromatography (IMAC) with Ni-NTA charged magnetic beads (Qiagen). Magnetic beads with bound cell lysate were washed with 200 μL of washing buffer for three times (150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... Primary-pools of ants were homogenized with 2 ceramic beads (2.8mm) in 350 µl QIAzol Lysis Reagent (Qiagen) for 2×2min at 30Hz in a TissueLyser II (Qiagen) ...
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bioRxiv - Bioengineering 2022Quote: Samples for ICP-MS were later thawed and homogenized in a TissueLyser II bead mill (Qiagen, Germantown, MD) at 30 Hz for 3 min with the addition of 1 μl deionized water per 1 mg organ tissue ...