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Citations for Qiagen :
951 - 1000 of 10000+ citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated from C2C12 myoblasts transfected with GFP-empty vector or GFP-Δ(1-240)JPh1 using the RNeasy-plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and resuspended in 350μl of Buffer RLT Plus supplemented with 1% (v/v) β-mercaptoethanol from the RNeasy Plus Mini Kit (Qiagen, 74134). RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: DNA was extracted from NCI-PC35-1 and NCI-PC35-2 organoids using an AllPrep DNA/RNA Mini Kit (Qiagen 80204) according to the manufacturer’s protocol for animal cells ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Zoology 2023Quote: ... The mixtures were incubated at 56 °C for 1 h before DNA was extracted using an QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was cut from a 1 % agarose gel and purified using the QIAGEN PCR purification kit (QIAGEN, Hilden, Germany). Afterwards ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... The digested vectors were run on 1% agarose gel and the target band was cut and harvested using QIAquick Gel Extraction Kit (QIAGEN 12362). The gel extraction products were further purified with QIAGEN MinElute PCR purification kit (QIAGEN 28004) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from BCBL-1-RTA at the indicated times following reactivation using the Dneasy blood and tissue kit following manufacturer guidelines (Qiagen 69581). 20ng of total DNA was used for qPCR using primers for the KSHV gene ORF57 ...
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bioRxiv - Biophysics 2024Quote: ... Cleavage products were separated on a 1% agarose gel and the linearised plasmid was purified with a QIAquick Gel Extraction Kit (Qiagen, #28706). Complementary synthetic DNA oligos coding for miR-34a target sites (Table S1) ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from OSU-CLL cells (1.5e6 cells/mL) treated with DMSO or SpiD3 (1, 2 μM; 4 h) using the miRNeasy Mini Kit (Qiagen; Hilden, Germany) per manufacturer instructions and processed using the Universal Plus mRNA-Seq with NuQuant kit (Tecan ...
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bioRxiv - Biophysics 2020Quote: ... All proteins were purified by affinity chromatography on Ni-NTA agarose (Qiagen), followed by incubation with TEV protease at 4 °C coupled with dialysis into buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of these tagged proteins was performed using Ni-NTA beads (Qiagen) or Glutathione Sepharose™ 4B beads (GE healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Functional analysis of differentially abundant proteins was performed with IPA (QIAGEN Inc.)[14] ...
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bioRxiv - Microbiology 2021Quote: ... 100 µL of washed magnetic beads (BioMag Protein A, Qiagen, Hilden, Germany) resuspended in 100 µL TN-buffer were added to each sample (total volume of 750 µL ...
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bioRxiv - Immunology 2020Quote: ... Protein purification was undertaken using nickel-affinity chromatography (Qiagen, Valencia, CA, USA) and endotoxin was removed by two-phase extraction with Triton X-114 22 ...
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bioRxiv - Biophysics 2022Quote: ... Protein was purified through affinity chromatography (Ni-NTA His-Bind resin, Qiagen), followed by gel filtration chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Cell Biology 2019Quote: ... Both proteins were affinity purified using Ni-NTA agarose beads (Qiagen, 30250) followed by gel filtration with a Superdex 200 HiLoad 16/600 column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... Fluorescent protein was bound to Ni-NTA Superflow columns (Qiagen, Hilden, Germany) equilibrated with 50 mM NaH2PO4 ...
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bioRxiv - Molecular Biology 2019Quote: ... The eluted protein complexes were further purified by Ni2+–NTA (Qiagen, Germany) affinity and/or anti-Flag M2 affinity chromatography (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted protein was incubated with pre-equilibrated Strep-Tactin Superflow Plus (Qiagen) and gentle rotation at 4°C for 1 h in binding buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved protein was further purified by reloading into Ni-NTA resin (QIAGEN). The flow through was collected ...
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bioRxiv - Biophysics 2020Quote: ... and the fusion protein was purified using a Ni-NTA resin (Qiagen). MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen) ...
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bioRxiv - Biophysics 2022Quote: ... and proteins were purified by two successive nickel affinity chromatography (Qiagen®) steps ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified by Ni-affinity chromatography (Ni-NTA agarose, Qiagen) followed by StrepTactin affinity chromatography (StrepTactin Sepharose high performance ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was purified by Ni-NTA affinity chromatography column (QIAGEN).
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bioRxiv - Biophysics 2022Quote: ... The solubilized protein was incubated with Ni-NTA agarose (QIAGEN, Hilden, Germany) for several hours ...
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bioRxiv - Microbiology 2022Quote: ... 6XHis-tag fusion proteins were purified using Ni-NTA affinity chromatography (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The 6xHis tagged fusion protein was purified with Ni-NTA Agarose (Qiagen), as described in [13] ...
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bioRxiv - Microbiology 2021Quote: Proteins were purified by gravity flow using Ni-NTA agarose (Qiagen, 30210) in 2 mL chromatography columns (Bio Rad ...
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bioRxiv - Genomics 2022Quote: ... 300 μl of Protein Kinase Digestion (PKD) buffer (Qiagen, Cat. No. 169021771) was added to sample tubes ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant protein in the supernatant was purified via Ni2+-NTA (Qiagen, UK) column [20].
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CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... His-tagged proteins were purified by Ni-NTA agarose affinity chromatography (Qiagen) and treated with TEV protease to remove the tag ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged protein purification was done with Ni-NTA agarose resin (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... the supernatants containing denatured proteins were mixed with Ni-NTA resin (Qiagen) and incubated for 60 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... His-tagged proteins were purified using a nickel column purification step (QIAGEN). Following IMAC ...
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bioRxiv - Plant Biology 2021Quote: ... The MBP/His-tagged proteins were purified using Ni2+-NTA agarose (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... the enriched proteins were incubated with 0.5 U/μl RNase A (Qiagen) and 20 U/μl RNase T1 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Proteases were precipitated using 200 ul of Protein Precipitation Solution (Qiagen, 158910) and centrifuged for 10 min at 12 000 g at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Cell Biology 2021Quote: ... 500-800μg of protein were incubated with Ni-NTA agarose beads (Qiagen) at 4° C overnight ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were purified from lysates on Ni-NTA columns (Qiagen).
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bioRxiv - Cell Biology 2020Quote: ... HIS-TRIM39 proteins were purified on Ni-NTA agarose beads (Qiagen, #1018244) and then eluted in a buffer containing 0.5 M imidazole before dialysis in PBS.
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bioRxiv - Biochemistry 2021Quote: ... Protein was first purified by FPLC using a Ni-NTA column (Qiagen) followed by a heparin column (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... bound His-mSTIC2 protein was detected by anti His HRP conjugate (Qiagen) and ECL reaction (Pierce).
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bioRxiv - Microbiology 2022Quote: ... The His-tagged ACE2 protein was then purified by Ni-NTA (QIAGEN) affinity purification ...
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bioRxiv - Plant Biology 2022Quote: ... His-tag proteins were purified using a Ni-NTA purification system (Qiagen) by following the specification of the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... The cleaved proteins were passed over a 1mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...