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Citations for Qiagen :
1201 - 1250 of 10000+ citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Preparation of his-tagged recombinant proteins was performed according to manufacturer instructions (Qiagen). Preparation of GST-tagged recombinant proteins was performed according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... The protein was purified under non-denaturing conditions using Ni-NTA resin (Qiagen) at 4 °C according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Digested protein was mixed with 3 ml of NTA super-flow resin (Qiagen) that had been pre-equilibrated in wash buffer ...
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bioRxiv - Physiology 2023Quote: ... Tissues were then lysed in protein isolation buffer using a tissue-lyser (Qiagen). Protein concentration was measured by Bradford assay ...
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bioRxiv - Biophysics 2023Quote: ... His6-MBP-HNRNPH2 proteins were first purified using Ni-NTA Agarose (#30230, Qiagen) and eluted with buffer containing 250 mM imidazole pH 7.8 (#79227 ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Nickel sepharose according to the manufacturer’s instructions (QIAGEN). The purified recombinant protein was dialyzed overnight against dialysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by immobilized metal affinity chromatography using Ni–NTA agarose (Qiagen) pre-equilibrated with lysis buffer in individual Econo-Pac gravity-flow columns (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: SAINT Express protein hits were analyzed using Ingenuity Pathway Analysis (IPA) software (Qiagen). Significant hits were analyzed and enrichment z-score was calculated for different pathways ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant TDP-43 proteins were purified over Ni-NTA agarose beads (Qiagen) and eluted using 50 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... All nucleotide and protein sequences were analyzed using CLC Main Workbench 8.1.2 (Qiagen).
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bioRxiv - Bioengineering 2024Quote: ... Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen) and eluted with DPBS containing 150 mM imidazole.
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bioRxiv - Neuroscience 2023Quote: Protein pathway analysis was conducted on DEP using Ingenuity Pathway Analysis (IPA, Qiagen) and Gene Ontology (GO ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen; Table S4), eluted with 50 mM Tris-base ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of the template was used in a 25 μL reaction using the QuantiFast Probe RT-PCR Kit (Qiagen, Inc., Valencia, CA) with 0.4 μM of each primer and 0.2 μM probe ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were checked on 1% agarose gels before being purified using a QiaQuick gel extraction kit (Qiagen, Inc., Valencia, California, USA) and directly sequenced in both directions using the amplification primers on an ABI 3730 automated sequencer (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from the supernatant of Vero E6 cells after 1 passage using the QIAamp Viral RNA Mini kit (Qiagen, https://www.qiagen.com/us/) without addition of carrier RNA ...
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bioRxiv - Genetics 2022Quote: ... The tissue powder was then transferred to a 1.5 mL centrifuge tube where 400 μL of 1% PVP-40 Buffer AP1 solution and 4 μL of RNase A were added (Qiagen DNeasy Plant Kit Qiagen Inc, Hilden, Germany). The tube was initially vortexed to homogenize the solution before incubation at 65 °C for 30 minutes with a short vortex every 5 minutes.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 1 μg of total RNA was used for cDNA conversion using the RT2 first strand kit (Cat# 330404, Qiagen, Valencia, CA, USA). The prepared cDNA was diluted and used to determine the expression of genes of interest using specific primers ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 female and/or 1 male per litter) were homogenised and total RNA was extracted using RNeasy Mini Kits (liver; Qiagen, Chadstone, VIC, Australia) or Trizol reagent (muscle and adipose tissue ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA from untreated or RSL3-treated (with or without 2 hr pre-treatment with 2 µM Ferrostatin-1) MM1S and MM1R cells was extracted using the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Genomic DNA (gDNA) was extracted from 1-month-old Arabidopsis thaliana rosette leaves with the DNeasy plant mini kit (Qiagen, cat. no. 69104) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from GFP-empty vector-transfected and GFP-Δ(1-240) JPh1-transfected C2C12 myoblasts using the RNeasy plus mini kit (Qiagen, Hilden, Germany), based on manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5μl of diluted cDNA (1:3 - nuclease-free water) was used as template in 10μl real-time PCR reactions (Qiagen: ‘SYBR Green PCR kit’) to assay specific transcripts (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Neuroscience 2023Quote: ... The DNA was separated on a 1% agarose gel and the appropriate band was excised and isolated using a gel extraction kit (Qiagen, Valencia, CA, USA). In-Fusion HD enzyme premix was added to the linearized PCR product and transformed into the Stellar™ Competent Cells (Takara ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We verified the PCR products by 1% agarose electrophoresis with EtBr staining and purified using the QiaQuick PCR Purification Kit (Qiagen, Hilden, Germany, 28104) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were washed once with 1×PBS after which DNA was isolated using the QIAamp DNA Mini Kit (Qiagen, Venlo, The Netherlands) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The specific bands were purified using gel purification kit (Qiagen gel purification kit). All these DNA amplified products were γ-32P radiolabelled by using T4 polynucleotide kinase enzyme using [γ-32P] ATP as the substrate with the reaction condition of 37°C for 30 min followed by 65°C for 25min enzyme inactivation period ...
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bioRxiv - Bioengineering 2019Quote: ... Total RNA extraction was performed using a standard kit (RNeasy mini kit, Qiagen). RNA concentration was quantified (ND-1000 ...
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bioRxiv - Bioengineering 2020Quote: ... DNA was extracted using a commercially available kit (Qiagen DNeasy Blood & Tissue Kit). Afterwards ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA was extracted using a commercial kit (RNeasy Micro kit, Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... DNA was extracted using a commercial kit (Qiagen Buccal Cell and DNAeasy Kit), as per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and using the RNeasy Mini kit and the RNAase free DNAase kit (Qiagen). After RNA extraction ...
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bioRxiv - Microbiology 2020Quote: We used the DNeasy PowerWater Kit and the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturer’s instructions to extract DNA from the water and soil samples ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated using RNeasy Micro Kit or RNeasy Mini Kit (Qiagen). After initial quality control using Agilent’s Bioanalyzer sequencing libraries were prepared from 500ng of total RNA per sample following Roche’s “KAPA stranded mRNA Seq” library preparation protocol for single indexed Illumina libraries ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated with RNeasy Micro Kit or RNeasy Mini Kit (Qiagen). cDNA was synthesized using 1μg of mRNA with SuperScript™ III Reverse Transcriptase (Invitrogen 18080044) ...
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bioRxiv - Neuroscience 2021Quote: ... Bacterial DNA was extracted using a specific kit (QIAamp Powerfecal DNA kit, Qiagen), and its concentration was quantified by Nanodrop 2000 C Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: Total RNA was isolated using RNeasy Mini kit or RNeasy Micro kit (QIAGEN). cDNA was synthesized with Oligo(dT ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNAs were purified using QIAfilter Plasmid kits (Midi pre kit, Qiagen #12243) following manufacturer protocol ...
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bioRxiv - Genetics 2023Quote: Total genomic DNA was extracted using a commercial kit (DNeasy Tissue Kit, QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... RNA was isolated via commercially available kit (Qiagen, RNeasy Mini Kit Cat# 74104) by lysing the cells in the vessel as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting RNA was purified using a spin-column kit (RNeasy mini kit, QIAGEN) and 120 pmol of sgRNA were complexed with 100 pmol of recombinant SpCas9 protein at RT for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: RNAs were isolated using the RNeasy mini kit mirVana RNA isolation kit (Qiagen). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... and RNA was extracted using a commercially available kit (QIAGEN RNeasy mini kit). RNA was stored at −80°C until submission to the University of Minnesota Genomics Center (UMGC).
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bioRxiv - Physiology 2023Quote: ... Total RNA was isolated with an RNA purification kit (Qiagen miRNeasy mini kit) and DNase treatment to remove DNA contamination.
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bioRxiv - Evolutionary Biology 2024Quote: High molecular DNA was extracted using the kit MagAttract HMW DNA kit (Qiagen) following manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2020Quote: ... Frozen cells were disrupted by bead beating at 30 h/z for 1 min before purifying total RNA using an RNeasy® plant mini kit (Qiagen™; ref. 74904) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: DENV viral RNA extraction was performed from acute serum samples (Baseline, 1-10, and 15 dpi) using QIAamp Viral RNA mini kit (Qiagen Inc, Valencia, CA, USA) according to the manufacturer’s instructions ...