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Citations for Qiagen :
51 - 100 of 220 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... A neighbour-joining tree was built using the Jukes-Cantor nucleotide distance model and 1,000 bootstraps in CLC Sequence Viewer 7 (Qiagen).
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bioRxiv - Neuroscience 2021Quote: ... and regulatory network construction was performed for each mouse model using Ingenuity Pathway Analysis (IPA, QIAGEN Bioinformatics, version 52912811). Fold-change values for each protein in the PSD proteome was used as input for a Core Analysis in IPA using the following default settings ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from 24 model communities (600 μL of each community) using the DNeasy PowerSoil Pro Kit (QIAGEN) according to manufacturers’ instruction ...
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bioRxiv - Microbiology 2021Quote: ... and a DeNovix® dsDNA Broad Range Assay Kit following the manufacturer’s instructions.Total genomic DNA was isolated from a subset of test diets using the DNeasy PowerMax® Soil kit (Qiagen, Hilden, Germany) to assess our ability to identify C ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA quality assessment of test slides was determined by first extracting RNA using the Qiagen RNeasy FFPE kit (#73504, Qiagen, Hilden, Germany) and measuring RNA concentration using a Qubit fluorometer (#Q33238 ...
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bioRxiv - Microbiology 2023Quote: ... a 1 mL aliquot was sterile filtered and kept to test conditioned media for IL-10 induction (as above)) and replaced with 5 mL of RNA-protect (Qiagen, Cat. # 1018390), mixed by pipetting ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... substances that did not contain training data sets for model building (Table 1) were further explored using Ingenuity Pathway Analysis (IPA) (QIAGEN Inc. ...
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bioRxiv - Immunology 2020Quote: Differentially activated biological pathways and upstream regulators were assessed for model factors of interest using the Ingenuity Pathway Analysis software (IPA; QIAGEN Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... a protocol and a linear regression model based on fluorometry measurements of dsDNA and ssDNA with Qubit (Qiagen, Hilden, Germany) were developed and validated ...
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bioRxiv - Bioengineering 2024Quote: ... library preparation was performed using gDNA extracted from untreated cells of three ACTA2 R179H MSMDS-fibroblast lines and liver from a mouse model of MSMDS using the Gentra PureGene Tissue Kit (Qiagen). Approximately 800 ng of purified gDNA per CHANGE-seq reaction was tagmented with a custom Tn5-transposome27 to an average length of 500 bp ...
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bioRxiv - Cancer Biology 2020Quote: ... We fit the model taking into account both RNA isolation methods (Qiagen™ Trizol™ and Qiagen™ All Prep™) and stages of prostate cancer progression (BPH ...
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bioRxiv - Cancer Biology 2020Quote: ... We fit the model taking into account both RNA isolation methods (Qiagen™ Trizol™ and Qiagen™ All Prep™) and stages of prostate cancer progression (BPH ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 M Tris/Bicine pH 8.5 with 9 µL of JCSG+ (Qiagen) condition #29 containing 0.8 M sodium phosphate monobasic monohydrate ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were homogenized using a tissue homogenizer (Brinkmann Instruments, Model PT 10/35, 110 Volts, 6 Amps, 60 Hz) for spleen and thymus while RLT (Qiagen, Hilden, Germany) and hand homogenization was used to isolate BM ...
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bioRxiv - Neuroscience 2020Quote: ... Differentially expressed genes (DEGs) were identified by gene-wise negative binomial generalized linear models using the EdgeR feature in CLC Genomics Workbench (Qiagen, version 10.1.1). The generated list was filtered based on ≥ 2x absolute fold change and false discovery rate (FDR ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA from both RMS tissues and models were extracted using the Allprep DNA/RNA/miRNA universal kit (Qiagen, cat. no. 80224) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA extractions using higher sediment volumes (up to 10 grams) with Qiagen DNeasy PowerMax Soil led also to low DNA yields (similar ranges as those reported for Qiagen DNAeasy Powersoil Pro kit tests). Because higher volumes of sediment also increase the concentrations of inhibitory compounds found in Guaymas subsurface sediments ...
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bioRxiv - Biochemistry 2022Quote: ... The peptide spot membrane was blotted onto a nitrocellulose membrane and UNC-45 binding was probed with an anti-Strep antibody (Qiagen) using an ECL-kit for detection (GE Healthcare).
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bioRxiv - Bioengineering 2020Quote: ... 0.001 M of HCl at pH 3 was added and then homogenized (TissueRuptor, Qiagen) on ice ...
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bioRxiv - Plant Biology 2020Quote: ... and ground for one minute at 20 m/s in a TissueLyser II (QIAGEN). Then ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from M-MDSCs using the miRNeasy Mini Kit (Qiagen, Germany). RNA libraries were prepared using TruSeq stranded mRNA sample prep kit (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription for cDNA synthesis was performed using M-MuLV Reverse Transcriptase (Enzymatics, Qiagen) and RNAse Inhibitor (Biotechrabbit) ...
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bioRxiv - Immunology 2024Quote: ... Cells were lysed in transwells by adding 350 μl RTL lysis buffer to the membrane surface with pipetting to wash the membrane surface and collect lysate (Qiagen, Germany). For FACs experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 ml of Matrigel basement membrane matrix (Qiagen #354234) prepared in serum free culture medium (1:8 ratio to reach final concentration of 1.2 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Solubilised membranes were incubated with Ni-NTA resin (Qiagen) (1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Then the membranes were DNase treated (DNase I, Qiagen) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... on-membrane DNase I (RNase-Free DNase Set, Qiagen) digestion was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 PPKS/M and 4 PPKS/Y) using the AllPrep DNA/RNA Mini Kit (Qiagen). Indexed libraries were generates using the KAPA mRNA HyperPrep kit and sequenced on a NextSeq500 sequencer (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Bioengineering 2021Quote: RNA was extracted from ∼1 M cells using the QIAGEN RNeasy Mini Kit (QIAGEN 74104). A total of 36 samples were prepared ...
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bioRxiv - Cell Biology 2020Quote: Membranes were blocked in Blocking Solution (PentaHis Kit, Qiagen, #34460) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... transwell membranes were submerged in 375 µl Buffer RLT (Qiagen) and cell lysate was frozen at -80°C for future RNA extraction.
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bioRxiv - Molecular Biology 2021Quote: ... STAT3 (M-003544-02) or non-targeting AllStars negative control siRNA (1027280) were purchased from Qiagen or Dharmacon ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated in 0.08 M KOH for 5 min and washed by Buffer EB (Qiagen). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Biochemistry 2024Quote: ... KCl (to a final concentration of 0.3 M) and 2 ml of Ni-NTA beads (Qiagen) were added and incubated at 40 °C for 1.5 h ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Systems Biology 2022Quote: ... 12 μl of 1 M Tris-HCl (pH 6.5) and 1 μl RNAse A (Qiagen cat # 19101) were added to the sample and incubated for 30 min at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and homogenized twice at 10 s at 2 M/s with a 5-mm steal bead (Qiagen) using a tissue homogenizer (MP Biomedicals) ...
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bioRxiv - Genomics 2021Quote: ... One section was added to a MACs M tube (Miltenyi Biotech, U.K.) containing 1ml Qiazol (Qiagen, U.K.) and dissociated on a GentleMACs (Miltenyi Biotech ...
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bioRxiv - Microbiology 2023Quote: ... followed by rigorous shaking for 40s at 5.5 m/s in a Tissuelyser II (Qiagen, Hilden, Germany). Serial dilutions of these samples were then plated on agar-solidified culture media (Fig ...
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bioRxiv - Biochemistry 2022Quote: IQN17 was crystallized at room temperature in a hanging-drop vapor diffusion system by mixing 0.3 μL of 15 mg/mL peptide with 0.3 μL of reservoir solution containing 0.1 M imidazole (pH 8.0) and 35 % 2-methyl-2,4-pentanediol (MPD) (Qiagen). Crystals were seen two days after trays were set up ...
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bioRxiv - Genetics 2024Quote: ... and M-Vac filters using DNeasy® PowerSoil® Pro Kit (Catalog number 47016, QIAGEN, Hilden, Germany) with some minor modification to steps 1-4 of the manufacturer’s protocol (DNeasy PowerSoil Pro Kit Handbook 06/2023) ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was treated with Penta-His MAb (Qiagen, Germantown, MD) followed by HRP-conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Neuroscience 2020Quote: Plcg2 knockdown was achieved in M-MØP cells using an antisense LNA GapmeR produced against mouse Plcg2 (Qiagen). Cells were grown to 80% confluence (∼2.4 × 105 cells per well ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... Magnetic beads (Dynabeads M-270 Carboxylic Acid) were activated and coated with ad hoc designed LNA probes (Qiagen), harboring an −NH2 group ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Cancer Biology 2020Quote: ... SAMHD1-targeting siRNA pool (siGENOME SMARTpool; M-013950-00-0005, Dharmacon) or control siRNA (AllStars Negative Control, Qiagen) were transfected at 10 nM final concentration.