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Citations for Qiagen :
1 - 50 of 220 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... vapor-diffusion crystallization trials were conducted using the JCSG Core I-IV screens (Qiagen), setup using a Mosquito Crystal (TTP ...
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bioRxiv - Biochemistry 2024Quote: ... Initial crystals were obtained via sitting drop vapor diffusion from the PEGs Suite (Qiagen) in 96 well plates and optimized with hanging drop vapor diffusion in 24 well plates ...
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bioRxiv - Microbiology 2021Quote: ... crystallization was performed by the hanging-drop vapor diffusion method using EasyXtal 15-well tools (QIAGEN). CA-NTD 1-146/Δ87-99G proteins were expressed and purified as described above ...
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bioRxiv - Microbiology 2020Quote: ... The clinical Samples (cervical smear) for the HPV DNA test were processed using HPV Test Hybrid Capture® 2 protocol (QIAGEN). Samples with relative light units (RLU ...
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bioRxiv - Developmental Biology 2022Quote: ... Differentially expressed genes were identified using the Proportional Estimation test based on Kal’s test feature in CLCGWB (Qiagen, Redwood City, CA) using the raw read counts for our bulk RNAseq datasets ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.5 mM CaCl2 by the sitting-drop vapour-diffusion method using the JCSG+ (Qiagen, Hilden, Germany), Index (Hampton Research ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from 800 μl residual material per sample from Abbott m2000 diagnostic tests using QIAamp mini DNA kit (Qiagen). Samples were quantified using plasmid and genome targets by quantitative PCR as previously described.30 Samples with ≥ 10 genome copies per μl of DNA were considered for WGS.
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bioRxiv - Immunology 2024Quote: ... Protein content was quantified using a BCA test (Qiagen). Legendplex assay was done as per the manufacturer’s instructions using a X20 Special Order 5-laser Fortessa (BD ...
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bioRxiv - Biochemistry 2023Quote: ... Screening plates were set up by a sitting-drop vapor diffusion method using JCSG Core I-IV (Qiagen), PEG Suite I-II (Qiagen) ...
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bioRxiv - Biochemistry 2023Quote: Initial crystallization conditions were identified utilizing hanging drop vapor diffusion with the Classics II and ProComplex screens (QIAGEN). The best crystals were obtained by equilibrating a 0.6 μL drop at 12 mg/mL of the complex of ARL15 (32-197 ...
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bioRxiv - Biochemistry 2024Quote: Initial crystallization conditions were identified utilizing hanging drop vapor diffusion with the Classics II and Nucleix screens (QIAGEN). The best LARP1 LaM domain/RNA complex crystals were obtained by equilibrating a 0.6 µL drop of the LaM domain (residues 323-410 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from the SBMA OL cell model and the control cell model using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The quality and quantity of the RNA obtained were checked using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... For test experiments bacterial DNA was extracted using genomic tip (QIAGEN). Extracted DNA was sheared to approximately 12 kb using g-Tube (Covaris) ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... The CLC gene set enrichment test (version 1.2, Qiagen, Venlo, Netherlands) was done with default parameters and based on the GO term ’biological process’ (H ...
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bioRxiv - Immunology 2021Quote: ... and subsequent crystallization optimization was performed manually using hanging-drop vapor diffusion method with 15-well screw-cap crystallization plates (Qiagen). The H5.28-HA head complex was crystallized in 7-9% PEG 8000 ...
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bioRxiv - Biochemistry 2024Quote: ... Initial screening was conducted using the sitting-drop vapor diffusion method with the ARI Crystal Gryphon Robot (ARI) and crystallization screening kits (QIAGEN) at 18°C ...
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bioRxiv - Microbiology 2020Quote: ... DNA from the 0.1 μm and pooled 0.2 μm membrane filters with iron flocculates was extracted using PowerMax Soil DNA Extraction Kit (Qiagen, Hilden, Germany), DNA was precipitated overnight and cleaned with 70% ethanol ...
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bioRxiv - Physiology 2020Quote: ... and Student’s t tests for significance per the manufacturer’s analytical software (Qiagen), with results plotted as fold change showing 95% confidence limits ...
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bioRxiv - Biochemistry 2023Quote: ... the initial crystallization screening was performed using the sitting-drop vapor-diffusion method with Screen classics and Classics II crystallization kits (Qiagen, Hilden, Germany) and PACT kits (Molecular Dimensions ...
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bioRxiv - Biophysics 2023Quote: ... were first obtained using the sitting drop vapor diffusion method using high-throughput crystallization screening kits (Hampton Research, Molecular Dimensions and QIAGEN). Crystals were then grown in a mixed solution containing 1 μl complex solution and 1 μl of reservoir solution using the hanging drop vapor diffusion method at 16°C ...
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bioRxiv - Molecular Biology 2024Quote: ... with oligo-mannose N-glycans were obtained with the help of the Max Planck Institute of Biochemistry (MPIB) Crystallization Facility by the sitting-drop vapor diffusion method using the Classics (Qiagen, Hilden, Germany), Index
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bioRxiv - Microbiology 2022Quote: Test and control samples were extracted using the QIAamp DNA Mini kit (Qiagen) with small modifications ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 M Tris (pH 7.0) (QIAGEN), 30% PEG 3000 (QIAGEN ...
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bioRxiv - Molecular Biology 2020Quote: ... The complex was initially crystallized in a buffer 0.1 M MES pH 6.5 and 0.6 M sodium fluoride from Anions Suite crystallization screen (Qiagen). After optimization of crystallization conditions ...
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bioRxiv - Plant Biology 2024Quote: ... and mapping of reads to gene models were performed using CLC Genomics Workbench (Qiagen). Reads were aligned to the SL4.00 genome release ...
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bioRxiv - Plant Biology 2021Quote: ... ANCOVA was performed to test the effect of the extraction method (Qiagen kit vs. PTB-DTT), and of the taxonomy on the DNA yield (DNA concentration) ...
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bioRxiv - Genomics 2020Quote: ... RNA was extracted from test tissue sections from each animal using the RNeasy Pico Kit (Qiagen) and yielded high RIN values (9.6 and 9.7 ...
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bioRxiv - Plant Biology 2023Quote: ... EDGE tests for analysis of RNA-Seq datasets were performed with CLC Genomics Workbench (Qiagen Informatics) as described earlier (Günther et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Differentially upregulated and downregulated genes were also identified with the Wilcoxon rank-sum test (min.pct = 0.1, only.pos = FALSE) and used for IPA analysis (Qiagen).
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bioRxiv - Biochemistry 2021Quote: ... 0.9 M KOAc [pH 4.8] (Qiagen N3 buffer). Centrifuge sample at 23°C for 10 minutes at 14,000 x g ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 M KOAc [pH 4.8] (Qiagen N3 buffer). Centrifuge sample at 23ºC for 10 minutes at 14,000 x g ...
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Bioengineering 2023Quote: ... The RT-qPCR experiments used Student’s t-test of the experimental group compared to the sham control (Qiagen GeneGlobe RT2 Profiler PCR Data Analysis) ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA of SPK and SPK model cells was extracted using RNeasy kit (Qiagen, Valencia, CA) and quantified with NanoDrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: gDNA was isolated from human sarcoma models and controls using the QIAamp DNA Mini Kit (Qiagen). Library preparation was performed by the Functional Genomics Laboratory (FGL) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from human sarcoma models and controls using the RNeasy Mini Kit (Qiagen). Library preparation was performed by the Functional Genomics Laboratory (FGL) ...
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bioRxiv - Genetics 2021Quote: ... About 50 million cells were collected and total RNA was extracted using the RNA Stat-60 (Tel-Test) according to the manufacturer’s protocol and quantified on a NanoDrop2000c and further cleaned using RNeasy kit (Qiagen). RNA-seq libraries were prepared from 500ng of RNA samples using an oligo dT based method with 15 cycles of PCR amplification with the Illumina TruSeq mRNA stranded kit (Illumina ...
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bioRxiv - Genetics 2021Quote: ... About 50 million cells were collected and total RNA was extracted using the RNA Stat-60 (Tel-Test) according to the manufacturer’s protocol and quantified on a NanoDrop2000c and further cleaned using RNeasy kit (Qiagen). rRNA was removed using the Ribo-Zero kit (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... The differential genes at an adjusted p value cut-off of 0.05 (Wilcoxon test) provided with p-value and fold change were imported into IPA (QIAGEN Inc. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The pathway enrichment analysis was performed using a hypergeometric test with Benjamini-Hochberg correction in Ingenuity pathway analysis (Qiagen). False discovery rate <0.05 was used as cutoff to identify significant signaling pathways after treatment.
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bioRxiv - Cell Biology 2024Quote: ... and differential expression was analyzed using the Empirical Analysis of DGE tool (edgeR test) in the CLC Main Workbench (Version 22.0; Qiagen). DEGs were extracted among conditions (pAV-mCherry vs ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 M Tris/HCl pH 8.5 (JCSG+ suite, Qiagen). Hanging drops of 1 ml protein at 2 mg/ml and 1 ml of precipitant (19% (w/v ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and mean TPM value between uninjured tails and tail blastemas were compared with a two-tailed t-test using the CLC genomic workbench with default parameters (Qiagen). A transcript or HHGC was deemed differentially expressed when its fold-change is greater than 2 or less than −2 and the FDR adjusted P-value < 0.05 ...
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bioRxiv - Microbiology 2022Quote: ... villosum) (Table 1) were used in RT-PCR tests conducted in Slovenia using the OneStepTM RT-PCR kit (Qiagen, USA) as previously described (Rivarez et al ...
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bioRxiv - Microbiology 2022Quote: ... Left lungs were harvested in 2 ml Eppendorf (Stevenage, UK) Safe-Lock micro test tubes containing 500 µl DMEM and a stainless-steel carbide bead (Qiagen), and snap frozen on dry ice until virus titration ...
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bioRxiv - Molecular Biology 2023Quote: All tests were run in triplicate and on a Rotor-Gene Q 2plex real time PCR Platform from QIAGEN (Germany).
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bioRxiv - Genomics 2022Quote: ... Differential expression analysis was performed across all group comparisons using the edgeR exact test as implemented in the CLC Genomics Workbench (Qiagen), and differentially expressed genes (DEGs ...
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bioRxiv - Cell Biology 2023Quote: ... significantly regulated human proteins (t-test, p-value ≤ 0.01; described above) were characterized by core analysis in IPA® software (version 68752261, QIAGEN Inc. ...
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bioRxiv - Systems Biology 2024Quote: ... mapping of reads to the potato genome (PGSC v4.03 genome model) 89 and read counting using the CLC genomics workbench v12.0 (Qiagen). Differential expression analysis was performed using a standard R package DESeq2 v1.42.1 90 workflow ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from one adult that was used as a control in 2.2 Insecticide Efficacy Tests using RNeasy Mini Kit (Qiagen, Hilden, Germany). The extraction was performed in triplicate for both the susceptible and resistant strains ...