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Citations for Qiagen :
51 - 100 of 238 citations for Complement Factor B CFB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from sorted B cells for each patient using the RNeasy Micro kit (Qiagen) with on-column DNase digestion ...
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bioRxiv - Microbiology 2023Quote: ... gDNA was extracted using an isopropanol-ethanol purification kit (Puregene Yeast/Bact. Kit B from Qiagen) and following the manufacturer’s protocol including a 60-minute RNase A treatment ...
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bioRxiv - Cancer Biology 2019Quote: RNA from CLL samples and B-cells was isolated by the RNAeasy® Mini kit by Qiagen. cDNA was synthesized using oligo-dT ...
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bioRxiv - Immunology 2022Quote: Total mRNA was isolated from A20 cells and primary B lymphocytes using a RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from MACS-sorted splenic B cells with the RNA Mini Kit (Qiagen, Hilden, Germany). Quantification of RNA was performed with a NanoPhotometer (Implen ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from enriched B cells using the RNeasy Micro Kit (Qiagen, cat no. 74004) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated from B-cells or mice tissue using RNA isolation kit (Qiagen, Valencia, CA) and then converted to complementary DNA using TaqMan Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNA was extracted from the B cells using the RNeasy Mini Kit (Cat. #74004, QIAGEN, Germany) as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were stimulated as indicated and RNA was isolated using RNeasy Plus Mini Kit (Qiagen). RT-PCR was carried out as described8 using the following primers ...
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bioRxiv - Systems Biology 2019Quote: ... B cells from an individual experiment were pooled and used to isolate RNA with RNeasy Mini kit (QIAGEN). After treatment with Ambion Turbo DNase ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from B cells either using Blood and Tissue or Flexigene kits (Qiagen, Hilden, Germany). DNA was quantified with Qubit (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted from naïve and cultured B cells using Trizol and RNeasy kit and Dnase treatment (Qiagen) and retro-transcribed to cDNA using random hexamers (Roche ...
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bioRxiv - Immunology 2020Quote: DAFhi and DAFlo GC B cells (CD19+ CD20+ CD38+ IgD−) were resuspended in RLT cell lysis buffer (Qiagen) after flow cytometric sorting ...
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bioRxiv - Genetics 2019Quote: DNA was extracted from blood or cell lines using the Puregene Blood Core Kit B (Qiagen, Cat#158467). PCYT1A exons were PCR-amplified from SMD-CRD patient genomic DNA with Accuprime Taq polymerase (Invitrogen ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from FO and MZ B cells using the RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... ∼12,000-15,000 B cells from the tetramer-enriched fraction were sorted directly into Buffer RLT (Qiagen; Hilden, DE) prior to shipment on dry ice to iRepertoire ...
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bioRxiv - Physiology 2024Quote: ... using 350 microliter RLT buffer with 1% b-mercaptoethanol as a collection/lysis buffer (RNeasy micro kit, Qiagen). RNA was extracted following manufacturer’s instructions (RNeasy micro kit ...
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bioRxiv - Molecular Biology 2023Quote: Inhibitors (antimiRs) against miR26b-5p and miR200a/b/c-3p were purchased from Qiagen (339130 and 339160 respectively). AntimiRs in injection media (1mM Tris-HCL pH 7.5 and 0.5 mM EDTA in embryo grade water ...
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bioRxiv - Bioengineering 2021Quote: ... Four hundred microliters of precipitation buffer B from the Qiagen (Formerly, Exiqon) miRCURY Exosome Isolation Kit (Qiagen, Germantown, MD) was added to the supernatant in each tube ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and 3.5 ml of RLT buffer with B-mercaptoethanol (10 µl for every 350 µl of RLT buffer – Qiagen) was added ...
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bioRxiv - Genetics 2020Quote: Total genomic DNA of the tigecycline-resistant isolate was extracted by Puregene Yeast/Bact Kit B (Qiagen, Maryland, US), and was sequenced by using Hiseq 4000 system (Illumina ...
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bioRxiv - Immunology 2020Quote: ... Gene ontology analyses for high affinity/SHM class-specific GC B cells were performed with Ingenuity Pathway Analysis (Qiagen) software using avg_logFC values of all genes significantly enriched in at least one class ...
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bioRxiv - Immunology 2023Quote: Genomic DNA from primary B cells treated with sg05 RNPs was isolated using the DNeasy Blood & Tissue Kit (Qiagen) and amplified for sequencing by nested PCR using Platinum SuperFi II Master Mix (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... and GFP+Ly5.1+ (OCA-B-expressing) cells were sorted by FACS and used for RNA purification (RNeasy Mini Kit, QIAGEN). RNA concentrations were determined using a Quant-iT RNA assay kit and a Qubit fluorometer (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... GC B cells (Live/Dead-CD19+IgD-CD95+GL-7+) and naïve B cells (Live/Dead-CD19+IgD+) were flow sorted into RLT Plus buffer (Qiagen) following pre-enrichment with the Pan B Cell Isolation Kit II (Miltenyi) ...
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bioRxiv - Immunology 2021Quote: RNA was prepared from sorted GC B cells and LNPCs from FNA or enriched BMPCs from bone marrow using the RNeasy Plus Micro kit (Qiagen). Libraries were prepared using the NEBNext Immune Sequencing Kit for Human (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: Genomic DNAs of tet(X)-positive isolates were extracted by using Puregene Yeast/Bact Kit B (Qiagen, Gaithersburg, MD, Germany) according to the instruction of the manufacture ...
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bioRxiv - Microbiology 2022Quote: ... Cytochrome b products were excised and purified from the gel using a commercial kit (QIAquick Gel Extraction Kit, Qiagen, Germany), followed by purification of eluted DNA using AMPure XP Magnetic Beads (1X ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in buffer A supplemented with 8 M guanidine hydrochloride (= buffer B) and loaded onto an Ni-NTA agarose column (QIAGEN) pre-equilibrated in the same buffer ...
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bioRxiv - Immunology 2019Quote: ... Two times 2.5 × 105 of naive B cells (CD20+IgM+IgD+IgG−) were sorted and subjected to RNA isolation (RNeasy Micro Kit; Qiagen). Following the generation of cDNA using 5’RACE (rapid amplification of cDNA-ends ...
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bioRxiv - Immunology 2020Quote: ... b is the intercept-y of the standard curve and m is the slope of the standard curve (QIAGEN, 2014). Subsequently ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and CD45+ Calcein+ DAPI-CD19+ CD38+ activated B cells were single-cell sorted into 96-well plates with catching buffer (RLT lysis buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2021Quote: Total RNA from sorted GC B-cells of immunized WT and CD22KO mice was extracted using an RNeasy micro kit (Qiagen). Indexed cDNA libraries were generated using a SMART-Seq Stranded kit (Takara Bio ...
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bioRxiv - Immunology 2021Quote: ... total RNA was isolated from 0.4×106 FACS-sorted B220hi CD138- and B220low CD138+ B cells (derived from 3 biological replicates after eight days in culture) using the RNeasy Mini Kit (Qiagen). cDNA was generated from polyadenylated transcripts employing the SMART-Seq v4 ultra low input RNA kit (Takara Bio) ...
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bioRxiv - Immunology 2022Quote: 2 × 103 to 1 × 105 sorted B cells per sample were centrifuged at 800g for 8min and total RNA was extracted using RNeasy Micro Kit (Qiagen) following the recommended protocol ...
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bioRxiv - Immunology 2022Quote: ... SEV and CTRL) at endpoints A and B (total of 30 samples) was extracted with QIAamp DNA Blood Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... transferred to 2 ml lysis matrix B tubes (MPBio) and subjected to bead beating for 15 min at 30 Hz (Tissuelyser II, Qiagen) with RNAse A added ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and proteins were extracted from fresh or snap-frozen FACS-sorted 2-5 million splenic B cells using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Aliquots of ∼500,000 isolated B cells were pelleted at 400g for 5 minutes and then resuspended in 350uL RLT Plus buffer (Qiagen). Total RNA was then isolated using RNeasy Mini Plus kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... of either the B cell clone or polyclonal B cells of the patients were directly FACS sorted into 100µL ALT lysis buffer (Qiagen MicroKit). gDNA was extracted as per manufacturer’s instructions (Qiagen MicroKit) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from FACS-sorted B220low/CD19+ splenocytes from sick mice (B-cell lymphoma only) using an RNeasy Micro kit (Qiagen). Control RNA was isolated from normal B220high/CD19+ splenocytes from control healthy mice that had also received pIpC and SRBC injections similarly to the experimental mice ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from FACS-sorted B220low/CD19+ splenocytes from sick mice (B-cell lymphoma only) using a RNeasy Micro kit (Qiagen). Control RNA was isolated from normal B220high/CD19+ splenocytes from control healthy mice that also received pIpC and SRBC injections similarly to the experimental mice ...
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bioRxiv - Microbiology 2022Quote: ... α-His antibody (Qiagen) was used at 1:5,000 dilution to detect the presence of rRH5 in Native-PAGE.
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bioRxiv - Cell Biology 2019Quote: ... KEN-Cyclin B1-GFP/mCherry-α-tubulin and GFP-Aurora B/WT-Cyclin B1-mCherry stable cell lines were established using Effectene (Qiagen) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: Whole RNA from sorted early EBV infected latency IIb B cells and from sorted donor matched LCLs were isolated using a RNeasy-Kit (Qiagen, #74104). mRNA sequencing libraries were prepared using a Kappa Stranded RNA-Seq Library Preparation Kit (Kappa Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA from sorted EBV infected early latency IIb proliferating B cells or sorted LCLs was isolated using an RNAeasy kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or remaining mosquito bodies were collected separately in a 2ml Eppendorf Safe Lock tube with 250μl DMEM (2% FBS, 1% Sodium Pyruvate) with 25 mg/ml Amphotericin B and a stainless steel bead (Qiagen, Hilden, Germany). Samples were stored at −80°C.
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD14- CD16- CD20+CD38+ BG505 SOSIP+YU2-gp140+ B cells were sorted into individual wells of a 96-well plates containing 5 μl of a lysis buffer (Qiagen, 1031576) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Bioengineering 2023Quote: Genomic DNA of sludge samples from the B-stage during the operation were extracted using the DNeasy PowerSoil Pro Kit (Qiagen, USA). The V4 region of the 16S rRNA gene was amplified using the primers 515F/806R and then the amplicons were sequenced on the Illumina’s MiSeq with V2 chemistry using paired-end (2 x 250 ...