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Citations for Qiagen :
1 - 50 of 238 citations for Complement Factor B CFB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... supernatant was collected and Factor Xa was removed with Factor Xa Removal Resin (QIAGEN). Endotoxin was removed using endotoxin removal resin and Atsttrin purity was determined by SDS-PAGE and reverse phase high performance liquid chromatography (HPLC).
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bioRxiv - Cell Biology 2023Quote: ... B) Proteinase K (Qiagen) diluted with PBS to 20 μg·ml-1 final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... or Puregene Core Kit B (Qiagen). Homozygously edited cells were identified using PCR and Sanger sequencing of gel extracted fragments (gel extraction performed using QIAquick Gel Extraction Kit from Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... Transcription factor prediction was performed with Ingenuity Pathway Analysis (Qiagen), iRegulon (Janky et al ...
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bioRxiv - Biochemistry 2023Quote: ... Co-factors were purified using Ni-NTA agarose beads (Qiagen), eluting with 300 mM imidazole ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... or (B) a QIAquick PCR purification column (Qiagen) (proK-col) ...
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bioRxiv - Genomics 2020Quote: ... and GeneRead Adapter I set B (Qiagen, cat # 180986) according to Qiagen protocol with one exception ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... and Negative control B Antisense LNA GapmeR (Qiagen, LG00000001) were used as controls for siRNAs and ASOs ...
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bioRxiv - Cancer Biology 2022Quote: A ready-to-transduce transcription factor-responsive lentiviral reporter system (CCS-1022L, QIAGEN) was used to generate a stable cell line ...
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bioRxiv - Immunology 2021Quote: B cell subsets were sorted directly into RLT buffer (Qiagen) with 1% mercaptoethanol and then snap-frozen in LN2 ...
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bioRxiv - Microbiology 2020Quote: ... 1A and B and lysed in RLT buffer (Qiagen, Germany) for RNA isolation ...
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bioRxiv - Immunology 2022Quote: ... B cell subsets were sorted directly into RLT buffer (Qiagen), and RNA isolated immediately ...
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bioRxiv - Physiology 2020Quote: ... or the QuantiTect PCR Kit with self-designed primers for neuronal factors (Qiagen, Germany) (Supplementary Table 3) ...
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bioRxiv - Immunology 2022Quote: ... Antigen specific B cells were gated with CD19+/IgM-/IgD-/Ghost dye-/PE+/BV605+ and sorted in catch buffer B (Qiagen TCL Buffer + 1% beta mercaptoethanol) by one cell per well in a 96 well plate ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A2 and B Wolbachia using PyroMark Assay Design 2.0 (Qiagen, USA). A complete list of primers sequences could be found in Table S3 ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using a Puregene Tissue Core Kit B (Qiagen).
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bioRxiv - Genetics 2019Quote: ... expand them and extract genomic DNA (Puregene Core Kit B, Qiagen) to improve the quality of the sequencing reaction ...
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bioRxiv - Molecular Biology 2020Quote: The consensus sequences of piRNA biogenesis factors were assembled using CLC Genomics Workbench version 8.0.1 (QIAGEN) and confirmed by published homolog sequences using BLASTp ...
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bioRxiv - Cancer Biology 2022Quote: ... The transcription factor prediction was performed using i-cisTarget20 and the upstream regulator function by QIAGEN Ingenuity Pathway Analysis.21 Differentially expressed genes (DEGs ...
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bioRxiv - Cancer Biology 2023Quote: ... with the addition of 1% B- mercaptoethanol using the TissueLyserII (85300, Qiagen) in combination with 5mm stainless steel beads (69989 ...
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bioRxiv - Genomics 2020Quote: ... and B cells) using the Qiagen AllPrep RNA/DNA kit (Qiagen, CA, USA). 500ng of DNA from each sample was treated with sodium bisulfite ...
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bioRxiv - Immunology 2019Quote: RNA was extracted from sorted B cells subpopulations using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was isolated from mouse B cells using the DNeasy kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... aureus genomic DNA was purified using the Puregene yeast/bacteria kit B (Qiagen). Lysostaphin ...
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bioRxiv - Immunology 2023Quote: ... Remaining B cells were lysed and mRNA was extracted using TurboCapture plates (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved B cells using the DNeasy Blood & Tissue Kit (Qiagen, cat # 69506). The Carrington Lab (National Cancer Institute ...
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bioRxiv - Immunology 2023Quote: ... B cell lysis and RNA extraction were performed using the RNeasy Kit (Qiagen), with the quality and concentration measured using Nanodrop (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... splenic B cells were collected for genomic DNA isolation using the DNeasy kit (Qiagen). Sμ–Sγ3 regions were amplified using nested PCR with details and primers as previously described (63) ...
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bioRxiv - Microbiology 2020Quote: DNA extraction was performed using the PureGene® Yeast/Bact kit B (Qiagen, Germany) following the manufacturer’s instructions for extraction of DNA from Gram-negative bacteria and eluted in molecular grade water.
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bioRxiv - Immunology 2022Quote: Total mRNA was isolated from primary B lymphocytes using a RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Total RNA was extracted from B cells using RNeasy Mini Kit (Qiagen, Valencia, CA). 100ng RNA was reverse transcribed using SuperScript IV VILO Master Mix (Thermofisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... or total IgDlo memory B cells were bulk sorted into Buffer RLT Plus (Qiagen) supplemented with 143 mM β- mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Total RNA of the sorted B cells was isolated with RNeasy Mini Kit (Qiagen). The libraries for RNA-Seq were sequenced using 2×75 bases paired-end protocol on an Illumina HiSeq 3000 instrument ...
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bioRxiv - Immunology 2023Quote: ... Cellular RNA was isolated from sorted B cell subsets with RNeasy Micro Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The lysates from the EmulsiFlex B-15 homogenizer were added to Ni-NTA agarose (Qiagen) and incubated on the nutator at 4 °C for 1 h ...
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bioRxiv - Immunology 2020Quote: ... total mRNA was isolated from mouse and human B cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Immunology 2022Quote: Sorted B cells were lysed for RNA extraction by the RNeasy Micro Kit (Qiagen, 74004). Primers used for reverse transcription and library amplification are provided in Table S2 ...
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bioRxiv - Physiology 2022Quote: ... The washed pellet was resuspended in Buffer B with 300 μg/ml DNase I (QIAGEN) and 5 mM MgCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells from the inserts were lysed and collected into RLT buffer containing B-mercaptoethnaol (QIAGEN) for downstream molecular analyses by RT-qPCR.
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bioRxiv - Plant Biology 2024Quote: ... CcAP1 and CcFULLike-A and CcFULLike-B with CLC Genomics Workbench v21.0.3 (QIAGEN, Aarhus, Denmark) (Supplementary Table S5) ...
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bioRxiv - Bioengineering 2024Quote: Sorted B cells were lysed for RNA extraction by the RNeasy Micro Kit (Qiagen, 74004). First-strand cDNA synthesis was performed on 8 μl of total RNA using 5 pmol of IgM and IgG specific primers in a 20 μl total reaction with SuperScript III (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: The heatmaps relative to the ERK-dependent AP-1 target genes were generated by interrogating the Transcription Factor Target (TFT) collection (MSigDB) and TF binding site database by QIAGEN with the top DEGs between control and PD-treated Acomys ...
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bioRxiv - Cell Biology 2019Quote: ... The copy number of standard DNA molecules was then determined by the following formula (from the script “Critical Factors for Successful Real Time PCR”, Qiagen):
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bioRxiv - Immunology 2020Quote: Differentially activated biological pathways and upstream regulators were assessed for model factors of interest using the Ingenuity Pathway Analysis software (IPA; QIAGEN Inc. ...
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LSD1 inhibition improves efficacy of adoptive T cell therapy by enhancing CD8+ T cell responsivenessbioRxiv - Immunology 2023Quote: ... Pathway analysis and transcription factor prediction analysis were performed with QIAGEN’s Ingenuity Pathway Analysis (IPA, QIAGEN Redwood City, www.qiagen.com/ingenuity).
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from fresh or cultured primary B cells using the RNeasy Minikit (Qiagen) or Trizol reagent (Ambion ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from the differentially stimulated B cells using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was prepared from human or mouse B cells using QIAmp DNA Mini Kit (Qiagen), or from paraffin-embedded human IgD or IgA myeloma tissue sections (obtained from the University of Arkansas for Medical Science ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from B cell populations sorted in duplicates (Gentra Puregene Core Kit, Qiagen). Rearranged IGHV genes were sequenced by massive parallel sequencing ...