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Citations for Qiagen :
51 - 100 of 161 citations for 8 sec Butyl quinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... gcy-8 mRNA was detected using a OneStep RT-PCR kit (QIAGEN) from 3 ng of template RNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 6 and 8 dpi using Qiazol and the RNeasy mini kit (Qiagen). Purified RNAs were processed using Turbo DNase (Ambion DNAfree kit) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5mM EDTA (pH 8) together with a 5 mm stainless steel bead (Qiagen) and macerated at 1,200 rpm for 2 mins in the Geno/grinder followed by a 10-minute centrifugation (17,000 x g ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After 8 h we isolated total RNA using a RNeasy Plus minikit (Qiagen). 384 TruSeq Stranded mRNA libraries were prepared in 96 sample batches ...
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bioRxiv - Developmental Biology 2020Quote: ... HF n=8) using the Qiagen RNeasy Plus Mini Kit (Qiagen, Toronto ON). RNA was quantified by spectrophotometry (DeNovix ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8) and 30 μl of 20 mg/ml Proteinase K (QIAGEN 19131) were added to the tissue/cell sample and incubated at 55°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was pooled and 8 ng was combined with SYBR Green Mastermix (Qiagen) for aliquoting into a 384-well RT2 Profiler PCR Array with genes from the Mouse Inflammatory Response and Autoimmunity set (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... Sequence files were processed in CLC Genomics Workbench version 8 (Qiagen Bioinformatics, Hilden, Germany); paired Illumina read files and 454 sequencing files were indicated during import ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was isolated from >8 bacterial colonies using QIAprep® Spin Miniprep Kit (Qiagen) and TRIM1 knockout confirmed by sequencing using a T3 forward promoter to confirm truncating stop codons in all copies of the TRIM1 gene present in the genome of selected clones ...
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bioRxiv - Microbiology 2020Quote: ... Sequences were aligned to the reference viral genome using CLC Sequence Viewer 8 (Qiagen).
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bioRxiv - Microbiology 2023Quote: RNA was extract from 8 x 106 cells using the RNeasy Mini Kit (Qiagen) protocol ...
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bioRxiv - Cancer Biology 2019Quote: Total RNAs were extracted from 8×105 cultured cells using RNA isolation kit (Qiagen, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... eight milliliters of culture was added to 8 ml of RNA Protect bacteria reagent (Qiagen) in 50-ml sterile tubes and then vortexed immediately for 5 sec and incubated at room temperature for 5 min ...
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bioRxiv - Genetics 2019Quote: ... Promoter sequences were assembled and aligned with CLC Main Workbench 8 software (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2021Quote: ... Sanger sequencing results were trimmed and assembled using CLC Main Workbench 8 (Qiagen, Hilden, Germany) using the default settings ...
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bioRxiv - Immunology 2022Quote: ... corpus (segment 6-7) and cauda (segment 8-10) samples using QIAzol Lysis Reagent (Qiagen) following the manufactureŕs recommendation using a bead-based tissue homogenizer (Retsch ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate was passed over a gravity column of 8 mL of Ni-NTA resin (Qiagen), washed with 70 mL wash buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 8 whole larvae using the QIAGEN RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Cell-free RNA was isolated from between 2-8 mL following manufacturer’s instructions (Qiagen, 55114). Isolated RNA was DNase-treated (Lucigen ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM imidazole) and was added to 8 mL Ni-NTA resin (Qiagen, Hilden, Germany) preequilibrated with lysis buffer and rocked for 1 hour at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... amplified with 8 cycles PCR using Kapa HiFi DNA polymerase and purified using GeneRead kit (Qiagen). Concentrations were determined using a Bioanalyser 7500 DNA chip ...
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bioRxiv - Neuroscience 2021Quote: ... the purification column was treated with 40μl of DNAse (diluted 1/8 in RDD Buffer) (Qiagen), incubated 15min and centrifuged at 8000×G for 15sec ...
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bioRxiv - Developmental Biology 2021Quote: ... The 8 reactions per sample were pooled and cleaned-up using QIAquick PCR Purification kit (QIAGEN). To remove the adaptor sequences from the resulting PCR products up to 2.5 μg of product was transferred to a new 1.5 ml tube and was digested with AlwI restriction enzyme (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... Mechanical disruption was performed using the TissueLyser II and 8-mm stainless steel beads (both Qiagen) at an amplitude of 30 for 2 min at a time ...
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bioRxiv - Cancer Biology 2020Quote: ... total miRNAs were extracted from 8-µm thick FFPE tissues by using a miRNeasy FFPE kit (Qiagen). Among them ...
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bioRxiv - Genomics 2021Quote: ... The cells were harvested 8-21d post-transfection and genomic DNA and RNA were immediately collected (Qiagen; DNeasy #69504 and RNeasy #74106 ...
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INTEGRATE: Model-based multi-omics data integration to characterize multi-level metabolic regulationbioRxiv - Systems Biology 2021Quote: Total RNA was extracted from at least 8 x 106 cells by using RNeasy Mini Kit (Qiagen). Each pellet was resuspended by adding 30 μL of RNAse-free water ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... 8 to 10 independent PCR reactions were pooled together and purified using the PCR purification kit (Qiagen). Multiplexed libraries were sequenced on Illumina HiSeq 2500 to obtain 100 bp single-end reads ...
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bioRxiv - Cancer Biology 2019Quote: Cell lysates were harvested following an 8 hour E2 or DMSO control induction with buffer RLT plus (Qiagen) containing 1% beta-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was extracted from HKC-8 or mouse kidneys using the miRNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Embryos and endosperms were collected 8 days after cross and total RNA was extracted using RNAeasy kit (Qiagen) plus in-column DNase digestion ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by phenol:chloroform:isoamylalcohol (25:24:1 pH 8) extraction followed by Qiaquick PCR Purification (QIAGEN, Germany). Purified DNA was then analyzed on a 1.5% agarose gel and on Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cSCC cell lines (n = 8) and NHEKs (n = 4) using miRNeasy Mini Kit (Qiagen), and the RNA-seq analysis was performed using Illumina HiSeq2500 system using paired-end sequencing chemistry with 100bp read length (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: DNA was isolated from the cells on day 8 after transduction using QiaAMP DNA blood mini kit (Qiagen) as per the manufacturer’s protocol and eluted in nuclease free water.
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bioRxiv - Biochemistry 2024Quote: ... ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO3 (pH 8.3) ...
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bioRxiv - Cancer Biology 2024Quote: ... pH = 8) and genomic DNA was isolated as described previously53 with the following modifications: QIAGEN Protease ((#19157, QIAGEN) and PureLink RNAse A ((#12091021 ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was stopped by adding 2.5 uL of 0.5M EDTA pH 8 and transposed DNA was purified using Qiagen MiniElute PCR purification kit (Qiagen). Purified DNA was amplified using the following condition ...
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bioRxiv - Zoology 2020Quote: ... Two independent replicates were performed in 8 μl of volume containing 0.5 U of Hot Start Taq polymerase (Qiagen), 0.18 μM of each primer and 0.04 mg of Bovine Serum Albumin Fraction V (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... and human BON1 cell line [8] were isolated with RNeasy Plus Universal Kits (Qiagen Cat no. 73404, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The solubilized membranes were centrifuged in a Sorvall WX ultracentrifuge (~109,000 g; 45 min, F50L-8×39 rotor) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated at 6 time points (0, 0.5, 1, 2, 4, and 8 hours) using the RNeasy Mini kit (Qiagen) according to the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... and 8 M urea [pH 8.0]) and His-tagged Tax proteins were precipitated with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen). After washing in buffer C (100 mM NaH2PO4 ...
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bioRxiv - Microbiology 2019Quote: ... 700 μL of cold methanol and 140 μL of EDTA 0.1M were added and vigorously mixed (4 x 45 s) in a mini-bead-beater-8 (Biospec Products, Qiagen). By this way ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 8 were transfected with a pool of cytotoxic siRNAs (“AllStars” wells, positive control, Allstars maximal death control, Qiagen). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The pure genomic DNA was sheared by ultrasound and 2-8 kb fragments were extracted from a 0.7% agarose gel using the Gel Extraction Kit (Qiagen). The gel-recovered fragments were ligated to the linearized PZE12 vector (prime 3,4 ...
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bioRxiv - Genetics 2021Quote: Relative telomere length of MDS patients was measured by quantitative polymerase chain reaction (qPCR) as previously reported.8 K562 cell DNA was extracted using the QIAamp Blood Mini kit (Qiagen). Telomere length was measured by two orthogonal methods ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pH 5.8) under long day condtions (16h light) at 24 °C (day) 22 °C (night) using the DNeasy Plant Kit (Qiagen). ONSEN copy numbers were determined by qPCR using 12 ng total DNA using the KAPA SYBR FAST master mix universal on a C1000 Touch (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... after adding 1.0 x 10^8 copies/μL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...