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Citations for Qiagen :
1 - 50 of 161 citations for 8 sec Butyl quinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... and end-point PCRs were performed following 34 cycles (94 C 30 sec, 58 C 30 sec, 72 C 1 min) using the HotStarTaq Plus DNA polymerase (Qiagen, Canada) according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... 1µg/ml Aprotinin, 1mM PMSF) using 5mm Stainless Beads and TissueLyser II (frequency: 30/sec, duration 2 × 30 sec) (Qiagen, Hilden, Germany). IL-23 was measured using either a mouse IL-23 ELISA (R&D ...
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bioRxiv - Neuroscience 2024Quote: ... using a pestle and micro-tube homogenizer (for 30 sec) or TissueLyser LT (QIAGEN, Dusseldorf ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... the muscles were homogenized (2 × 30 sec, 30 Hz, TissueLyser II bead mill, Qiagen, USA) in a modified GSK3-buffer (10% glycerol ...
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bioRxiv - Microbiology 2021Quote: ... during two rounds of 30 sec at 5,000 rpm in a TissueLyser II grinder (Qiagen). Total RNA was extracted using the NucleoSpin 96 kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 8500×g for 30 sec and washed again with Buffer AW2 (Qiagen, 80204), and then centrifuged at full speed for 2 min ...
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bioRxiv - Systems Biology 2023Quote: ... paired-end (37:8:8:38) RNA-sequencing using a RNeasy Micro kit (Qiagen #74004) for RNA extraction ...
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bioRxiv - Cell Biology 2020Quote: All muscles were homogenized 2 × 30 sec at 30 Hz using a Tissuelyser II (Qiagen, USA) in ice-cold homogenization buffer (10% (v/v ...
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bioRxiv - Immunology 2020Quote: ... Disruption of microbial cells was enhanced using TissueLyser II (QIAGEN, 30 shakes/sec., 1 min., 2x). To lyse Gram-negative bacteria ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from each SEC fraction by miRNeasy serum/plasma kit (Qiagen 1071073 Lot#160020206) after adding 1.0 x 10^8 copies/μL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035) ...
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bioRxiv - Immunology 2020Quote: ... Mechanical disruption of microbial cells was done using TissueLyser II (QIAGEN, 30 shakes/sec., 10-20 min.) and the samples were centrifuged (10,000 g ...
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bioRxiv - Cell Biology 2019Quote: ... All muscle were homogenized 2 x 30 sec at 30 Hz using a Tissuelyser II (Qiagen, USA) in ice-cold homogenization buffer (10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 min 30 sec at 30 Hz before RNA extraction using the RNeasy micro kit (Qiagen). RNA was sequenced using the DNBseq platform at the Beijing Genomics Institute (BGI Group ...
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bioRxiv - Plant Biology 2023Quote: ... twice for 30 sec at 30 Hz before RNA extraction using the RNeasy Plant Mini kit (Qiagen). For RT-qPCR ...
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bioRxiv - Bioengineering 2022Quote: ... We conducted standard bulk paired-end (37:8:8:38) RNA sequencing using RNeasy Micro (Qiagen, 74004) for RNA extraction ...
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bioRxiv - Systems Biology 2021Quote: We conducted standard bulk paired-end (37:8:8:38) RNA sequencing using RNeasy Micro (Qiagen 74004) for RNA extraction ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue–methanol mixture was subjected to bead-beating for 45 sec using a Tissuelyser cell disrupter (Qiagen). Extracts were centrifuged for 5 min at 5,000 rpm to pellet insoluble material and supernatants were transferred to clean tubes ...
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bioRxiv - Cancer Biology 2019Quote: ... diluted 1:8 in PKD buffer (Qiagen) at 56°C for 1 hour with interval shaking ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted 1:8 in PKD buffer (Qiagen) at 56°C for 1 hour with interval shaking ...
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bioRxiv - Microbiology 2023Quote: TG was quantified in snap-frozen liver tissue stored at −80 □C until cryo-grinding in liquid nitrogen and 50 ±5 mg tissue added 0.9 mL of a 2:1 chloroform:methanol solution and homogenized 1 min at 50 os/sec using a TissueLyser LT (Qiagen) with beads ...
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bioRxiv - Microbiology 2020Quote: DNA extractions from the 8-week fecal samples (n = 8 per group) were performed using the Qiagen QIAmp DNA stool extraction kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by adding 8 µl Effectene Enhancer (Qiagen), vortexing and incubation for 5 min ...
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bioRxiv - Microbiology 2020Quote: Oral fluid samples were prepared by homogenization of 200 μl sample (30 sec at 15 Hz) in a Tissuelyser II (Qiagen) followed by centrifugation (2 min at 10.000 rpm) ...
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bioRxiv - Microbiology 2019Quote: ... for sample preparation each Nucleospin Tube Type A containing 100 mg of feces was loaded into pre-chilled adapters and shaken twice for 30 sec with a TissueLyser II instrument (Qiagen) at a frequency of 25 Hz ...
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bioRxiv - Microbiology 2021Quote: ... was added to each tube and samples were homogenised twice at 25 Hz for 30 sec using a TissueLyser II (85300; QIAGEN) and centrifuged for 2 min at 2,000 x g ...
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bioRxiv - Genetics 2019Quote: ... Frozen spores were lysed using cryogenic bead beating with two 5 mm steel beads shaking twice for 45 sec at 25 Hz using TissueLyser II (Qiagen). Lysis buffer was added to the frozen broken spore pellet ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... 45 cycles at 95° for 15 sec and 60° for 1 min were performed in the Rotor-Gene Q real-time PCR cycler (Qiagen). The numbers of copies of the RdRP gene in the samples were calculated using an RdRP cDNA standard curve.
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bioRxiv - Microbiology 2020Quote: ... Soil samples (8 g) preserved in LifeGuard solution (Qiagen) were thawed on ice and centrifuged at 2,500 x g for 5 minutes to collect the soil at the bottom of the tubes ...
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bioRxiv - Biochemistry 2020Quote: ... 8×105 cells with the RNeasy mini kit (Qiagen) in combination with the QIAshredder system (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: Sequences were imported into CLC Genomics Workbench 8 (QIAgen) and were mapped to the dd_Smed_v6 transcriptome (Brandl et al. ...
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bioRxiv - Microbiology 2020Quote: ... whereas 30 mg of lung tissue was homogenized in 600 μl RLT-buffer containing β–mercaptoethanol for 30 sec at 15 Hz using the TissueLyser II (Qiagen).
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bioRxiv - Bioengineering 2020Quote: ... The samples were ground by bead beating for 25 sec at 25 Hz using a Tissue Lyser II machine (Qiagen, Hilden, Germany). For CFU-based bacterial enumeration ...
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bioRxiv - Plant Biology 2023Quote: Samples were snap frozen in liquid nitrogen and pulverised in a tissue lyser at 30 Hz for 30 secs (Qiagen TissueLyser II). Anthocyanin pigments were extracted by adding acidic methanol (1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were thawed at the time of titration and homogenized for one minute at a frequency of 26 sec-1 in a TissueLyser (Qiagen, Haan, Germany) prior centrifugation for 5 minutes at 16,100xg to pellet debris ...
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bioRxiv - Molecular Biology 2019Quote: ... CLC Genomics Workbench 8 (Version 8.5.1, Qiagen, Germantown, MD, USA) was used to trim ...
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bioRxiv - Microbiology 2022Quote: ... thaliana leaves at 8 dpi using an RNeasy kit (QIAGEN). We amplified the candidate sequences for the genes of interest with PCR using Phusion® High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Systems Biology 2020Quote: ... IL-8 ELISA was performed according to manufacturer instructions (Qiagen).
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bioRxiv - Molecular Biology 2021Quote: 8 different polymerases (KAPA HiFi HotStart, Qiagen Taq, Q5 (NEB), Phusion Hot Start Flex DNA Polymerase (NEB) ...
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bioRxiv - Genetics 2023Quote: ... Lysate was passed over 8 ml Ni-NTA resin (Qiagen), washed with 70 ml wash buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... The Puregene Cell Kit (8×108) (Qiagen, Cat. No. 158767) was used for all genomic DNA (gDNA ...
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bioRxiv - Microbiology 2023Quote: ... supernatant was poured over 8 mL Ni-NTA resin (Qiagen), resin was washed with 35 mL lysis buffer supplemented with 1 M NaCl ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... The supernatant was transferred to clean 1.5mL MCF tube and to which 350 μL ethanol at 4’C was added before vortexing for 10 sec and transferring the supernatant to a QIAamp Mini spin column (Qiagen Germantown, MD U.S.A.). The sample was process as recommended in the QIAamp DNA Mini Handbook and eluted with 10 mM Tris-Cl ...
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bioRxiv - Microbiology 2019Quote: ... 8 ml of bed volume of Ni-NTA resin (Qiagen, Germany) was added and incubated for one hour with gentle agitation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequences were manually curated with CLC Main Workbench 8 software (Qiagen) before being aligneed with the Muscle program implemented in the workbench to generate haplotypes by line for each intron ...
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bioRxiv - Genomics 2021Quote: ... 8 and 10 of differentiation as recommended (RNAeasy Minikit. Qiagen 74106). Mineralized nodule formation was measured by staining cultures with Alizarin Red (40 mM ...
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bioRxiv - Microbiology 2022Quote: ... and lysate was passed over 8 mL Ni-NTA resin (Qiagen) using gravity chromatography ...
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bioRxiv - Immunology 2023Quote: ... and disrupted for 8 minutes in 300 μL ATL buffer (Qiagen) using a Qiagen Tissuelyser at 50Hz with a ceramic bead (MPBio) ...
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bioRxiv - Cancer Biology 2023Quote: ... 8 wells of cell death control (Qiagen AllStars Positive Control siRNA), and 8 wells of YAP targeting siRNA (Ambion Silencer Select ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was passed over 8 ml of Ni-NTA resin (Qiagen), and the resin was washed with 70 ml of lysis buffer supplemented to 1 M NaCl followed by 20 ml lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... and 8 h post-infection) using the QIAamp DNA micro kit (QIAGEN). As a parallel control ...