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Citations for Qiagen :
901 - 950 of 1518 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Nucleic acids were extracted from the homogenate using AllPrep DNA/RNA/miRNA Universal kit (Qiagen, Germany). The quantity and quality of DNA and RNA were analyzed using the NanoDrop 1000 spectrophotometer and its integrity verified by the Agilent 2100 BioAnalyzer.
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Microbiology 2022Quote: ... The unprotected nucleic acids were removed by adding 50 μL of 10x Turbo DNase Buffer (Qiagen), 5 μL of 2U/μL Turbo DNase (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were extracted from specimens using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Hilden, Germany), QIAamp Viral RNA Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: Hippocampal DNA was extracted using an automated nucleic acid extraction platform called QIA cube HT (Qiagen) with a column-based extraction kit ...
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bioRxiv - Genomics 2023Quote: ... Cell free DNA was isolated from plasma using the Qiagen Circulating Nucleic Acids Kit (Qiagen GmbH). For 29 patients ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and then subjected to total nucleic acid extraction using QIAamp viral RNA mini kit (Qiagen, Germany) according to manufacturer’s instruction without addition of carrier RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The nucleic acid fraction was then added to the column of a RNeasy microkit (Qiagen # 74004) and the manufacturer’s protocol was followed ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleic acids were then precipitated using ethanol and genomic DNA was removed using DNase treatment (Qiagen). RNA was quantified using Qubit (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant containing each target protein was loaded onto a Ni-NTA (nitrilotriacetic acid) column (Qiagen) which was pre-equilibrated with the corresponding Lysis Buffer ...
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bioRxiv - Microbiology 2024Quote: ... A volume of 200µl homogenate supernatant was used for nucleic acid extraction with the QIAcube (Qiagen) machine ...
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bioRxiv - Microbiology 2022Quote: ... Viral nucleic acids were extracted using the QIAMP® Viral RNA mini kit (Qiagen, Venlo, Netherlands) without addition of carrier RNA to the lysis buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Purification was performed on nickel-nitrilotriacetic acid (Ni-NTA) agarose using the QIAexpressionist™ kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Total ribonucleic acid (RNA) from the hippocampus was isolated with the RNeasy mini kit (©QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: All DNA extractions were completed with the QIAamp® Circulating Nucleic Acid Kit (Qiagen, catalogue # 55114) or with the QIAamp® MinElute ccfDNA Kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... WT and mutant proteins were initially purified using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAgen). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: Nucleic acid was extracted from each sample using QIAmp Viral RNA mini kits (Qiagen, Hilden, Germany) in accordance with manufacturer protocols ...
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bioRxiv - Physiology 2024Quote: ... RNA was reverse transcribed using miRCURY Locked Nucleic Acid (LNA™) Reverse Transcription (RT) kit (Qiagen) according to manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... The solubilized suspension was then applied to a nickel-nitrilotriacetic acid agarose (Ni-NTA agarose, QIAGEN) column ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 μg target vector using PolyFect transfection reagent (Qiagen, 301107). Media containing virus was collected both 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Virus Precipitation Solution (System Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... for 3 min at 50 Hz using a TissueLyser LT (Qiagen) with 1 min incubation on ice in between homogenisations ...
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bioRxiv - Microbiology 2020Quote: ... CjNC110-LNA DIG-labeled probe (/5’DigN/ GCACATCAGTTTCAT/3’Dig_N/) (QIAGEN) was added to 15 mL DIG EasyHyb™ Buffer (Roche ...
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bioRxiv - Systems Biology 2022Quote: ... 5 μl of proteinase K (approximately 3 U, Qiagen, cat. # 19131) were added and incubated for 1 h at 56°C ...
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bioRxiv - Neuroscience 2020Quote: ... siRNAs targeting the 3’UTR of Arhgap11a were purchased (siRNAflex, Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was incubated with 3 ml Ni-NTA resins (QIAGEN) with gentle agitation at 4 °C for an hour ...
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bioRxiv - Cell Biology 2021Quote: ... 5’-TAGCATTGTCAAGCTCCTAAA-3’ siRNAs or AllStars Negative Control siRNA (Qiagen, #1027281). After 72 h ...
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bioRxiv - Bioengineering 2023Quote: ... containing 3 μL 4X Probe PCR Master Mix (250102, Qiagen, USA) (final concentration 1X) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated with binding buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... using 30 nM Hs_LUZP1 siRNA (target sequence, 5′-CAGCGGGTGCTGAGAATTGAA-3′; QIAGEN) or 30 nM AllStars negative-control siRNA (QIAGEN) ...
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bioRxiv - Molecular Biology 2022Quote: ... Antisense LNA GapmeRs (custom designed, 3’-FAM-labeled, Qiagen, Table S5) were bilaterally microinfused using a 2 μl calibrated micropipette (Hamilton syringes ga 25/70mm/pst3) ...
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bioRxiv - Microbiology 2024Quote: ... The pellets were resuspended in 3 ml RNAprotect Bacteria Reagent (Qiagen), aliquoted ...
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bioRxiv - Microbiology 2024Quote: ... and 3) DNA extraction (using here DNeasy Blood & Tissue kit, Qiagen) from the pellet for molecular analysis to detect E ...
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bioRxiv - Cell Biology 2024Quote: ... HOXD11 (Qiagen, Sequence: 5′-TGC TAG CGA AGT CAG A-3′), HOXD13 (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... HOXD13 (Qiagen, Sequence: 5′-CAT CAG GAG ACA GTA T-3′), for 24hr and 48hr for RNA and protein extraction respectively ...
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bioRxiv - Cell Biology 2024Quote: ... 0.25 uL T7 Ligase (3×10^6 U/mL) (Qiagen Enzymatics), 15 ng purified amplicon ...
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bioRxiv - Plant Biology 2021Quote: ... Lyophilized material was pulverized in a 2ml screw top microcentrifuge tube with a glass bead for 10 minutes at a frequency of 20/s in a TissueLyser (Retsch ballmill, Qiagen, Venlo, Netherlands). The material was AIR prepped and destarched using protocols from Barnes and Anderson (Barnes and Anderson ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA from representative isolate(s) for each study participant was extracted using a QIAGEN DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany) and the 16S rRNA gene was amplified using the primers 63f and 1387r.53 Amplicons were purified enzymatically with ExoSAP-It (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: The DNA of all 52 samples was extracted from silica-gel dried leaves using the Qiagen DNeasy Plant Mini Kit following slightly modified manufacturer′s instructions (Qiagen, Valencia, CA). DNA quality and concentration were verified and quantified using NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: A region-specific polymerase chain reaction (PCR) was performed for the S-COMT promoter region using the PyroMark PCR Kit (Qiagen, Hilden, GER) according to manufacturer’s instructions with previously published primers (F ...
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bioRxiv - Microbiology 2021Quote: ... and a DeNovix® dsDNA Broad Range Assay Kit following the manufacturer’s instructions.Total genomic DNA was isolated from a subset of test diets using the DNeasy PowerMax® Soil kit (Qiagen, Hilden, Germany) to assess our ability to identify C ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated following the Powersoil DNeasy kit protocol with the addition of a 10 min bead beating step at 50 s-1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 45 cycles of denaturation at 95 °C for 15 s and annealing at 60 °C for 1 min following a melting step in a Rotor-Gene Q machine (Qiagen, Hilden, Germany). GAPDH was used for normalization ...
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bioRxiv - Cell Biology 2024Quote: ... tissue was snap frozen in liquid nitrogen and pulverized with a microdismembrator S (B. Braun Biotech, Melsungen, Germany) before total RNA isolation by means of the Lipid Tissue Mini Kit (Qiagen, Hilden, Germany). Isolated cells were lysed in RLT buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... along with 8 prickleless parental accession individuals (S. macrocarpon PI 441914) was extracted from young leaf tissue using a DNeasy Plant Pro Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions for high-polysaccharide content plant tissue ...
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bioRxiv - Microbiology 2024Quote: ... Insects were homogenized in 200 µl Schneider’s medium or phosphate-buffered saline (PBS) using one 5 mm stainless steel ball per midge and a TissueLyser (Qiagen, Hilden, Germany) agitating for one minute at 30 Hz ...
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bioRxiv - Genomics 2024Quote: ... we utilized the Integrative Genomics Viewer (IGV) v2.16.1[38] and the CLC workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Furthermore ...
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bioRxiv - Developmental Biology 2020Quote: Nucleic acid material remaining in ECM preparations was isolated using a DNeasy Blood & Tissue Kit (Qiagen, Germany). The amount of DNA was quantified using Qubit Fluorometric Quantification.
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bioRxiv - Bioengineering 2021Quote: The soluble fraction of the cell lysate was mixed with Ni2+-nitrilotriacetic acid agarose beads (Qiagen, USA), and the His6-tagged recombinant proteins were purified by immobilized metal affinity chromatography (IMAC ...
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Structural Basis for SARS-CoV-2 Envelope Protein in Recognition of Human Cell Junction Protein PALS1bioRxiv - Biochemistry 2021Quote: ... the supernatant was collected for affinity purification by nickel-nitrilotriacetic acid affinity chromatography (Ni-NTA, Superflow, Qiagen). The eluate was concentrated and buffer exchanged for tag removal by incubation with TEV protease overnight at 4 °C ...