Labshake search
Citations for Qiagen :
851 - 900 of 1518 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Viral nucleic acids were then isolated using QIAamp MinElute Virus Spin Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions with some modifications described (Cosentino et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... A locked nucleic acid probe (mmu-miR-450b-5p miRCURY LNA miRNA Detection probe; Qiagen) was used to detect miR-450b while standard DNA oligonucleotide probes were used for other miRNAs ...
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bioRxiv - Developmental Biology 2023Quote: DNA was extracted from blood on an automated nucleic acid extraction platform called QiaSymphony (Qiagen) with a magnetic bead-based extraction kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribonucleic acid (RNA) was extracted and purified using RNeasy RNA Isolation Kit (QIAGEN, Germantown, MD) then reverse transcribed into complementary deoxyribonucleic acid (cDNA ...
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bioRxiv - Genomics 2024Quote: ... CfDNA was extracted from the plasma samples using the QIAamp Circulating Nucleic Acid Kit (Qiagen) and approximately 3 – 8 ng of cfDNA was isolated from each plasma sample for use in the 5hmC-Seal assay ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Genomic nucleic acid isolation was performed using the MagAttract HMW DNA Kit (Qiagen, Hilden Germany) precisely following the instructions of the fresh or frozen tissue protocol ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Recombinant Fab proteins were purified from the culture supernatant by nickel-nitrilotriacetic acid agarose (Qiagen). Recombinant mAbs were affinity purified by MabSelect resin (Cytiva ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and nucleic acid was extracted using EZ1 Advanced XL extraction with a Virus Card (QIAGEN). Six of the 20 FACS populations did not generate sufficient DNA and were not subsequently sequenced ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by incubation with nickel-nitriloacetic acid (Ni-NTA) agarose resin (Qiagen) for 3h at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The clarified supernatant was loaded onto pre-equilibrated nickel-nitrilotriacetic acid (Ni-NTA) (Qiagen, USA) affinity column and flow-through was collected after incubation for 30 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was extracted using the Qiagen RNA nucleic acid extraction kit (Qiagen, Hilden, Germany) at 0- ...
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bioRxiv - Cancer Biology 2021Quote: ... a 19-mer SPRY2 target sequence (5′-AACACCAATGAGTACACAGAG-3) (QIAGEN) was used and for the control a 19-mer NSi control sequence (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... For Astrin siRNA oligo #367 (5′-TCCCGACAAC TCACAGAGAAA -3′)(Qiagen) was used as described (Thein et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... and 3 µg pINDUCER20-GFP-AFOS using PolyFect (Qiagen, 301107). Media was collected at 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Solution (System Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... Hoko-3 using a Genomic-tip kit (Qiagen, Hilden, Germany). Library preparation was performed using SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Biochemistry 2023Quote: ... and mixed with 3 mL of Ni-NTA resin (Qiagen) for 1.0 h at 4 °C and then applied to a gravity flow column ...
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bioRxiv - Molecular Biology 2024Quote: ... were crushed with a tungsten carbide bead (3 mm, Qiagen) on a Retsch MM400 mixer mill for 60 seconds at 30 Hz and DNA was extracted using the DNeasy Plant Mini kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... pCDNA-SARC-CoV-2 Wuhan-Hu-1 P681H S plasmid was then extracted using the QIAprep Spin Miniprep Kit (Qiagen N.V.) and Sanger sequencing was used to confirm incorporation of the mutation.
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bioRxiv - Microbiology 2021Quote: ... These cells were incubated overnight in MEM-α media free of FBS and supplemented with 1% P/S followed by collection of Supernatant (conditioned media) and total RNA extraction (Cat# 74134, Qiagen, Germany). The resulting samples were stored at −80°C until analyzed further in downstream applications.
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bioRxiv - Biochemistry 2021Quote: ... Transcriptomes of weed species commonly targeted by asulam in the field were assembled using CLC Genomics Workbench v20.0.3 (Qiagen Aarhus A/S) from data in the NCBI Sequence Read Archive ...
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bioRxiv - Epidemiology 2020Quote: ... Then a steel ball was added and samples were crushed twice during 2 min at 30 Hz/s with the Tissue Lyzer (Qiagen, Germany). 450 µL of fresh PBS 1X were added to the samples ...
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bioRxiv - Biochemistry 2021Quote: ... haageana were downloaded from the Sequence Read Archive (BioSample No. SAMN02933922)29 and a transcriptome assembled using CLC Genomics Workbench (version 21.0.3; QIAGEN Aarhus A/S). The reads were first trimmed using parameters Q=30 and minimum length 50 ...
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Genetic architecture of oxidative stress tolerance in the fungal wheat pathogen Zymoseptoria triticibioRxiv - Genetics 2020Quote: ... Sequence variation among the parental strains in the QTL confidence intervals was identified by performing sequence alignments (CLC Sequence Viewer 7.6.1, QIAGEN Aarhus A/S). The previously published in planta expression data (Palma-Guerrero et al ...
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bioRxiv - Genetics 2023Quote: ... The D-loop chromatograms were visually checked using Geneious 9.1.8 (Biomatters Ltd, Auckland, New Zealand) and sequences were aligned using CLC Sequence Viewer 7.6.1 (Qiagen Aarhus A/S, Aarhus, Denmark). DNA extracted were genotyped by GENOSCREEN ...
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bioRxiv - Microbiology 2023Quote: ... one aliquot was not processed any further and one other aliquot was bead beaten with 100 mg of sterile glass beads (400-600 nm) for 30 s at 30 Hz in a Tissue-lyser II (Qiagen, Germany), in order to open up Symbiodiniaceae cells and release intracellular bacteria to facilitate their growth on agar plates ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and homogenized at a frequency of 26/s for 1 minute in a TissueLyser II (Qiagen, Germantown, MD) and centrifuged at 16,500xg for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 viriants S and point-mutated pseudovirus were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Cat#52906), and served as template for reverse transcription using the TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR reagent (TransGen Biotech ...
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bioRxiv - Plant Biology 2022Quote: Two RNA samples from rosette leaves of after-bolting WT plants and bglu28/30-2 mutant plant grown under different S conditions and their 7-day-post-anthesis siliques were extracted with the RNeasy Plant Mini Kit (QIAGEN, Germany). The residual DNA was removed with DNase treatment (DNaseI ...
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bioRxiv - Genomics 2023Quote: ... and the obtained .nhx file was reformatted as .nwk file for further processing using CLC Mainworkbench (21.0.1, QIAGEN Aarhus A/S). Evolutionary analyses were conducted in MEGA X (Kumar et al ...
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bioRxiv - Plant Biology 2024Quote: Changes in gene expression were analyzed using Differential Expression in Two Groups method included in CLC Genomics Workbench 21.0.5 software (QIAGEN Aarhus A/S). One group was composed of SALK_116579 mutant ...
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bioRxiv - Immunology 2021Quote: Cell free DNA was extracted from 850μl serum using the QIAamp Circulating Nucleic Acid Kit (Qiagen) and was quantified by TaqMan Real-time PCR (StepOneTM Plus Real-Time PCR System ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was loaded onto a column packed with Ni(II)-nitriloriacetic acid agarose (Qiagen) pre-equilibrated in 100 mL of binding buffer (50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM PMSF and the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose (QIAGEN) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... lysed and incubated with nickel-nitrilotriacetic acid beads as per the manufacturer instructions (Qiagen, Hilden, Germany). Beads were washed ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acid was extracted using the Qiagen QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), substituting carrier RNA with linear polyacrylamide (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acids were isolated from cell lysates or lung homogenates using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the collected eluate was incubated with pre-washed nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen) for 90 min at 4°C to remove the His-tagged TEV protease ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from 2OD of cells using acid phenol and purified using RNeasy kit (Qiagen). Reverse transcription was performed on 750ng of RNA using SuperScript III First-Strand Synthesis SuperMix (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: HUVECs were transfected at 60% confluency with 10 nmol/L locked nucleic acid (LNA) GapmeRs (Qiagen) or small interfering RNAs (siRNAs ...
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bioRxiv - Microbiology 2022Quote: Viral nucleic acid was extracted from the tissue homogenates using QIAamp DNA mini kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... cfDNA was then extracted from human blood plasma using the QIAmp Circulating Nucleic Acids kit (Qiagen), eluted in 60-μl elution buffer (10 mM Tris-Cl ...
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bioRxiv - Microbiology 2021Quote: ... Total nucleic acid was extracted using the Qiagen QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), substituting carrier RNA with linear polyacrylamide (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted (MagMAX Total Nucleic Acid Isolation Kit, Life Technologies, Carlsbad, CA using a Qiagen robotic extraction station ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was first purified on a Ni–nitrilotriacetic acid (NTA) column (QIAGEN, Valencia, CA). The eluate was further purified on glutathione–Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Total nucleic acid extraction from nasopharyngeal specimens was performed using the QIAamp RNA Viral Kit (Qiagen), and two sets of primers were taken for two target genes (ie ...
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bioRxiv - Microbiology 2021Quote: ... Extracted nucleic acids were passed through the Allprep DNA/RNA Mini Kit (Qiagen Sciences, Maryland, USA) to separate RNA from DNA ...
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bioRxiv - Genomics 2020Quote: ... where it underwent nucleic acid extraction using the QIAamp Viral RNA mini kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instruction ...