Labshake search
Citations for Qiagen :
901 - 950 of 1256 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: DNA was isolated from all the cell lines using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). The samples were treated with sodium bisulfite to convert unmethylated cytosine residues to uracil via deamination using the EpiTect Bisulfite kit (Qiagen) ...
-
bioRxiv - Biochemistry 2023Quote: DNA was extracted from the insoluble protein pellet with the QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer protocol for DNA extraction from blood ...
-
bioRxiv - Neuroscience 2023Quote: ... The protein was then purified on Ni-NTA affinity chromatography with Ni-NTA agarose resin (Qiagen) following the protocol described in Qiagen ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant containing each target protein was loaded onto a Ni-NTA (nitrilotriacetic acid) column (Qiagen) which was pre-equilibrated with the corresponding Lysis Buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and the fusion proteins purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
-
bioRxiv - Immunology 2023Quote: ... and the supernatant was used for bacterial protein purification using Ni-NTA affinity resin (Qiagen: 30210).
-
bioRxiv - Microbiology 2024Quote: ... 1% v/v sodium dodecyl-sulfate pH 8.0) for protein extraction or in RLT buffer (Qiagen) for RNA extraction ...
-
bioRxiv - Biochemistry 2024Quote: ... WT and mutant proteins were initially purified using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAgen). Subsequently ...
-
bioRxiv - Synthetic Biology 2024Quote: The MED21 protein sequence was aligned to homologs using CLC Sequence Viewer 7 (QIAGEN, Aarhus, Denmark), a tree was constructed using a neighbor-joining method ...
-
bioRxiv - Biochemistry 2024Quote: ... His-NusA-FASP or His-NusA-Degron fusion proteins were purified using Ni-NTA resin (Qiagen) using standard approaches and His-TEV protease was added to cleave the His-NusA tag from the PER2 peptides on column at 4°C overnight ...
-
bioRxiv - Immunology 2024Quote: ... The eluted compounds were subjected to RNA and protein isolation using QIAzol (Qiagen, Misusage, ON, Canada) following the manufacturer’s instruction ...
-
bioRxiv - Immunology 2024Quote: ... The supernatant from the sonicated proteins was purified by using Ni-NTA agarose (Qiagen, Düsseldorf, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Proteins and complexes were purified from the cell supernatants using Nickel-NTA affinity resin (Qiagen: 30210), followed by Superose 6 (Cytiva ...
-
bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted from MGM tissue specimens using the AllPrep DNA/RNA/Protein Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The secreted recombinant NA proteins were purified from culture supernatant by Ni-NTA affinity chromatography (Qiagen) followed by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare ...
-
bioRxiv - Developmental Biology 2024Quote: ... the UAS vector encoding the protein and the ubiquitin-Gal4 construct were transfected with Effectene (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... The cells were transfected with 50 nM double-stranded siRNA oligonucleotides (Supplementary Table 3) using HiPerFect Transfection Reagent (Qiagen, Crawley, UK).
-
bioRxiv - Immunology 2021Quote: ARNO siRNA (Mm_Pscd2_3) or a negative non-specific siRNA control (Allstar siRNA) were transfected into SFs using HiPerFect transfection reagent (all Qiagen,UK) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2020Quote: ... 0.5 µl of diluted template cDNA (1:3, nuclease-free water) per real-time PCR reaction (10 µl – SYBR Green PCR kit, Qiagen, 204143) was used to assay specific transcript abundance (CFX96 Real-Time System ...
-
bioRxiv - Genomics 2022Quote: ... genomic DNA was extracted from 3-week-old leaves of ALO seedling using the QIAGEN® Genomic DNA Extraction Kit (Cat. 13323, Qiagen) according to the manufacturer protocol ...
-
bioRxiv - Physiology 2020Quote: ... RNA was isolated from maternal and fetal tissues (Table 3 and 4) using QIAamp cador pathogen mini kit (Qiagen, Valencia, CA). ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen ...
-
bioRxiv - Genomics 2021Quote: ... DNA corresponding to the size of mononucleosomes (100-200 bp) was re-isolated from a 3% agarose gel using MinElute Gel Extraction Kit (Qiagen, # 28604). As input ...
-
bioRxiv - Immunology 2021Quote: ... RNA was extracted from homogenized lysate containing a range of 3×103 to 1×105 cells per sample using a ll Prep DNA/RNA Mini kit (Qiagen, #80204). RNA was purified and concentrated using RNAClean XP beads (Beckman Coulter ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Approximately half the volume of each feces sample (100-200 mg) was immersed in 3 ml of Inhibit EX Buffer (QIAGEN, Hilden) and stored at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... The following primers were used to assess the relative level of GAT-3 mRNA: Slc6a11 Rn.10545 in comparison to that of cyclophilin A (Peptidylprolyl isomerase A) used as housekeeping gene (Qiagen, UK). Real-time-PCR was performed on the CFX96 Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... 200 μL of plasma/EV suspension were mixed with 1000 μL Qiazol and 1 μL of a mix of 3 synthetic spike-in controls (Qiagen, Germany). After a 10-minute incubation at room temperature ...
-
bioRxiv - Plant Biology 2020Quote: ... by shaking with two 3-mm glass beads for 2 min at 30 Hz with a Tissue-Lyser (Qiagen, Hilden, Germany). After 5 min in an ice-cold ultrasonic bath ...
-
bioRxiv - Systems Biology 2020Quote: RNA was isolated from 3 biological replicates of each U2OS time point according to manufacture instruction using the RNeasy Plus Mini Kit (QIAGEN, #74134). Isolated RNA was reversely transcribed by using first-strand cDNA synthesis kit (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed in an Applied Biosystems QuantStudio 3 thermocycler using the QuantiNova Probe SYBR Green PCR Kit (Qiagen, Germany); mix proportions and cycling parameters were used as described in manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... The lysate was then clarified by centrifugation at 16,000 x g for 45 minutes and then applied to 3 mL Ni-NTA resin (Qiagen, Valencia, CA) equilibrated with buffer A ...
-
bioRxiv - Microbiology 2022Quote: ... and the amplified DNA bands from the 5’ and 3’ ends were individually excised and purified with QIAquick® Gel Extraction Kit (QIAGEN). Purified PCR products were cloned into pJET1.2/blunt plasmid (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: 1-3 million of feeder-independent mESCs were harvested for genomic DNA extraction by using a QIAamp DNA Mini Kit (QIAGEN, 51306). Genomic DNA concentration was determined with a Nanodrop spectrophotometer ...
-
bioRxiv - Cell Biology 2022Quote: ... and organoids at the end of passage one subjected to two weeks of differentiation (n=3) using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 150 mg of cluster roots (3 to 4 roots) using the RNeasy Plant Mini Kit (Qiagen, 74904) and treated with the DNA-free DNA Removal Kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... 40 μl of media from each of 6 plant wells or media from 3 minimal media wells were pooled and stabilized in RNAprotect® Bacteria Reagent (QIAGEN) before performing RNA extraction using RNeasy Mini Kit (QIAGEN) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and RNA was purified after 3 days from the second transfection using the RNeasy Plus Mini Kit (Qiagen, Catalog number 74134) following the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2022Quote: ... Cells from 3 independent biological replicates of MG1655 in each modified medium were treated with RNA protect bacteria reagent (Qiagen, Germany), to prevent degradation of RNA ...
-
bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
-
bioRxiv - Epidemiology 2020Quote: ... then ticks were washed by gentle shaking over 2-3 min at 7 Hz/s in a Tissue Lyzer (Qiagen, Germany). After discarding the supernatant ...
-
bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from aerial parts of 3-week-old seedlings grown on soil using either RNeasy Plant Mini Kit (Qiagen, 74904) or Monarch Total RNA Miniprep Kit (NEB ...
-
bioRxiv - Biochemistry 2021Quote: ... RT-PCR products were gel purified in 3% agarose gel and cleaned up using QIAGen gel extraction kit (QiaGen, Hilden, Germany).
-
bioRxiv - Cell Biology 2021Quote: ... The plasmid DNA was extracted from 3-9 positive colonies where possible after blue-white screening using a QIAprep® Spin Miniprep Kit (Qiagen) and sequenced using the standard T7 primer at Eurofins Genomics ...
-
bioRxiv - Cancer Biology 2022Quote: ... and GSC6-27 HEPACAM shRNA (n=3) cultures were washed in cold 1X PBS and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, 74134) following manufacturer’s guidelines ...
-
bioRxiv - Biochemistry 2022Quote: ... Trimming of the obtained sequencing reads and mapping to the pea aphid genome (version 3) were performed with the program CLC Genomics Workbench (Qiagen Bioinformatics) (mapping parameter ...
-
bioRxiv - Bioengineering 2022Quote: ... A cell mass of an equivalent of a OD600 3 was used for a DNA mini preparation using a QIAprep® Spin Miniprep Kit (Qiagen). DNA was eluted in 50 μL elution buffer and measured using a NanoDrop one (Thermo Fisher Scientific).
-
bioRxiv - Genetics 2022Quote: ... and non-inoculated G305-3M leaf segments collected along 10 different time points (0, 3, 6, 9, 12, 16, 24, 36, 48, 72 hpi) using the RNeasy Plant Mini Kit (Qiagen, Germany). The cDNA was synthesized from total RNA using a qScript™ cDNA Synthesis Kit (Quantabio ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from NPCs and their derived astrocytes (4 lines, n=3 per cell type) using a RNeasy mini kit (Qiagen, 74104). RNA samples were prepped using TruSeq® Stranded mRNA Library kit (Illumina ...
-
bioRxiv - Neuroscience 2022Quote: ... neurons were transfected by lipofection at 3 days in vitro (DIV) with target siRNA or ntRNA using Effectene Transfection Reagent (Qiagen, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... or a non-targeting scrambled control (Scr; sequence: 5’-TAACACGTCTATACGCCCA-3; Qiagen, Cat No.: 339137; 0.1 nmol in 2 μL PBS), using a 2 μL Hamilton syringe at a rate of 1 μL/min ...