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Citations for Qiagen :
901 - 950 of 2209 citations for 8 3 5 DIMETHYL 4 METHOXYPHENYL 8 OXOOCTANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and pooled using the UltraClean 96 PCR Cleanup kit (Qiagen 12596-4) and Quant-iT dsDNA High Sensitivity Assay kit (Invitrogen Q33120) ...
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bioRxiv - Genomics 2022Quote: ... and 4 µl of 100 mg/ml RNase A (QIAGEN, cat# 19101) were added to the homogenate ...
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bioRxiv - Immunology 2022Quote: ... After diluting samples in a 4:1 ratio (elution buffer [Qiagen]:cDNA), cDNA concentration was determined using a Bioanalyzer (Agilent Technologies).
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bioRxiv - Biochemistry 2020Quote: ... Crystallization screening was performed at 4°C with JCSG core I (Qiagen) using the sitting drop vapor diffusion method ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl of DNA) and run in Rotor-Gene Q machine (Qiagen). Primer sequences are listed in the Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... Removed culture was mixed with 4 mL of RNAprotect Bacteria Reagent (Qiagen) for stabilization and incubated for 5 min at room temperature before centrifugation at 4000 rpm for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... with 4 × 108 copies of cel-miR-39 miRNA mimic (Qiagen 339390) spiked into to the sample after addition of lysis buffer.
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bioRxiv - Molecular Biology 2022Quote: ... HL-1 cells were transduced with a pool of 4 gapmers (Qiagen) at 40nM (10Nm each ...
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bioRxiv - Microbiology 2023Quote: ... The siRNAs were selected from the FlexiTube GeneSolution 4 siRNA sets (Qiagen) and transfected as a mix at 24 nM in Calu-3 and 10 nM in Caco-2 following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... At least 4 colonies were used for each DNA miniprep (QIAprep, Qiagen) and mutations were confirmed by Sanger sequencing (Source Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... at a frequency of 28/s @ 4°C (Tissue Lyser II, Qiagen). After this step the samples were adjusted to RT for 5 minutes and processed using the RNeasy Lipid Tissue Mini Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2023Quote: ... pH 7.5 at 4°C) and purified using the RNAeasy kit (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: ... and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen), which recognized the His-tag on PXDN-con4 ...
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bioRxiv - Genomics 2020Quote: ... 5 mL nuclease-free water) instead of customary Elution Buffer (Qiagen) (see Supplementary Material Methods) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.08 μl of 5 U/μl HotStarTaq DNA polymerase (Qiagen 203203) and 15.1 μl or 14.5 μl milliQ water ...
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bioRxiv - Neuroscience 2022Quote: ... STHdh cells were transfected with FlexiTube siRNA (5 nmol) from Qiagen (Mm_Csnk2a2 ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was incubated with 5 mL Ni-NTA resin (Qiagen) for 2 h ...
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bioRxiv - Cancer Biology 2019Quote: ... with the addition of a 5 mm stainless steel bead (Qiagen) and ice-cold 50% methanol/20 μM L-norvaline (450 μl) ...
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bioRxiv - Biochemistry 2020Quote: ... and bead-bashed for 5 min at RT (Qiagen TissueLyser II). Lipid extracts were prepared using a modified version of the Bligh and Dyer lipid extraction [70] ...
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bioRxiv - Microbiology 2019Quote: His Tag-Alexa 647 (5 ug/mL) (Qiagen, catalog no. 35370)
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bioRxiv - Microbiology 2019Quote: ... 0.125 μl Taq DNA Polymerase (5 units/L, Qiagen, Hilden, Germany) and 1 μl of DNA template ...
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bioRxiv - Immunology 2020Quote: ... which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed using 5-10 ml RBC lysis buffer (Qiagen) and stained for flow cytometry analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded on 5 mL Ni-NTA column (Qiagen) equilibrated with buffer C ...
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bioRxiv - Molecular Biology 2023Quote: ... DMSO or 5-aza-dC-treated cells (51304, Qiagen, DUS, GER). 200 ng DNA was dropped onto a positively charged Nylon membrane (Amersham Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM BME) and purified through Ni-NTA agarose beads (Qiagen) (Denaturing Elution Buffer ...
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bioRxiv - Bioengineering 2023Quote: ... on a 5-plex QIAcuity One digital PCR instrument (Qiagen, 911021). The thermal cycling conditions were implemented using the following program ...
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bioRxiv - Microbiology 2023Quote: ... the tissue was homogenized with stainless steel beads (5 mm; Qiagen) in a TissueLyser II (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mM BME) and purified through Ni-NTA agarose beads (Qiagen) (Denaturing Elution Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA Strep-tactin superflow (Qiagen, Germantown, MD, USA) columns were used ...
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bioRxiv - Microbiology 2024Quote: ... The incubated resin was loaded into 5 ml polypropylene column (Qiagen) subsequently multiple washes were applied using a wash buffer pH 8.0 (50 mM NaH2PO4 ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant containing solubilized protein was filtered using a 0.22 μM nylon membrane filter and incubated with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germantown, MD) at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids (DNA/RNA) were isolated from flash-frozen tissues using the All Prep DNA/RNA Mini Kit (Qiagen, Germantown, MD) as previously described [127–129] ...
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bioRxiv - Neuroscience 2019Quote: RNA was isolated from vehicle- or bile acid-treated microglia and astrocyte cultures using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). First-strand cDNA was synthesized by reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... double-digoxigenin locked nucleic acid probe (RNO-MIR-124-3P: CATTCACCGCGTGCCTTA, Tm: 84°C, 339111 YD00614870-BGC, miRCURY LNA™ miRNA Detection Probe, Qiagen) was used at a final concentration of 30 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed twice in PBS and incubated for 20 min at 54 °C (60 min at 52 °C for tissue sections) in mercury locked-nucleid acid (LNA) miRNA ISH Buffer 80 nM hybridiziation buffer (100 nM for tissue section) (Qiagen, 339450). Digoxigenin labeled miRCURY LNA probes (HSA-MIR-10B-5P ...
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bioRxiv - Microbiology 2021Quote: Samples were homogenized and nucleic acids were extracted using the MoBio RNA PowerSoil® Total RNA Isolation Kit (Qiagen, CA, USA) and the MoBio RNA PowerSoil® DNA Elution Accessory Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: Frozen tissues were weighed and homogenized in RLT and nucleic acids were extracted using the AllPrep DNA/RNA Mini Kit (QIAGEN, #80204) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation Kit (Thermo #AM1975) and concentrated using the RNeasy MinElute Cleanup Kit (Qiagen #74204). Library preparation and sequencing were performed by the Genome Sequencing Facility at St ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was divided into two parts: one part was directly combined with nickel-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen, China); the other part was heated in a 65 °C water bath for 60 minutes and then centrifuged at 14000 rpm for 60 minutes ...
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bioRxiv - Immunology 2021Quote: ... The plasmid was transfected to 293T cells and the recombinant S protein trimers were purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography (QIAGEN, Germany), followed by size exclusion to further purify the trimers ...
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bioRxiv - Microbiology 2022Quote: ... nucleic acid extractions were performed by combining equal amounts of cell culture supernatants with RLT Lysis Buffer (Qiagen, Germantown, MA, USA), with 200 µL of the lysate used for magnetic bead-based extraction according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was adjusted to 500 mM NaCl and 20 mM imidazole before affinity purification by incubation with nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, 30210) for 90 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: Lysates of His-tagged α-catenin WT and Δmod were bound to Ni-NTA (Ni2+-nitrilotriacetic acid)-sepharose affinity chromatography (Qiagen), washed with 50 mM Tris pH 7.8 ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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First complete genome characterization of an Indian pigeon pox virus directly from a clinical samplebioRxiv - Evolutionary Biology 2023Quote: ... The extraction of nucleic acid from infected scab samples was performed by using DNeasy blood and tissue purification Kits (QIAGEN, USA) with some modification established by Sarker et al. ...
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bioRxiv - Biochemistry 2023Quote: ... the EcNhaA triple mutant was extracted from membranes with n-Dodecyl β-D-maltoside (DDM; Glycon) and purified by Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) affinity chromatography ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from 3×106 neutrophils by using the RNeasy Mini Kit (Qiagen). TaqMan real-time quantitative RT-PCR was performed utilizing the Applied Biosystems StepOne Plus cycler (Applied Biosystems ...
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bioRxiv - Systems Biology 2020Quote: ... with 3 mm beads then extracted according to the protocol using RNeasy Mini Kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... vIL-6 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using QuantiTect Multiplex RT-PCR Kits (Qiagen) as described previously (37 ...