Labshake search
Citations for Qiagen :
851 - 900 of 1020 citations for Recombinant Human TGFB2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Proteins were then purified on Ni2+ -NTA affinity resin following a modified version of the manufacturer’s instructions (QIAGEN) [25] ...
-
bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
-
bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
-
bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
-
bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed on the eluted DNA samples at 50°C for 30 minutes (Qiagen Protease mix). ChIP DNA was purified using Sera-Mag beads (Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from the frozen placental tissue using the Protein and RNA Isolation (PARIS) kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The phage protein capsid was digested with Proteinase K and the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) was used to purify the DNA ...
-
bioRxiv - Cancer Biology 2019Quote: ... The lysates were clarified by centrifugation and proteins in the supernatant were purified using Ni-NTA chromatography (Qiagen) for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare ...
-
bioRxiv - Plant Biology 2019Quote: ... The protein prokaryotic expression and purification was according to the instruction of Ni-NTA Agarose (Cat.No.30210, QIAGEN) and Glutathione Sepharose (GE).
-
bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
-
bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
-
bioRxiv - Cell Biology 2020Quote: ... total RNA was isolated from 5 × 105 cells with the AllPrep© DNA/RNA/Protein Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted from samples using the AllPrep DNA/RNA/Protein Mini Kit (80004, Qiagen Sciences, Inc.) and 0.25 μg of total RNA was reverse transcribed by SuperScript™ IV First-Strand cDNA Synthesis System (18091050 ...
-
bioRxiv - Immunology 2021Quote: ... to provide Uniprot entries and further processed in Perseus (v1.6.6.1).18–20 Significantly regulated proteins were investigated in Ingenuity Pathway Analysis (v47547484, Qiagen, USA)
-
bioRxiv - Developmental Biology 2021Quote: Log fold-change values of the top 5% proteins were used as input for Ingenuity Pathway Analysis (Qiagen: https://digitalinsights.qiagen.com/products/features/) ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by sonication and N-terminus His6-tagged proteins were purified on Ni-NTA columns (Qiagen) according to manufacturer’s instruction ...
-
bioRxiv - Immunology 2020Quote: ... and the cleaved His6-MBP tag was removed by passing the protein through an amylose resin column (Qiagen). The flow-through fraction containing the tag-free protein was further purified using a Superdex 200 (10/300 GL ...
-
bioRxiv - Biophysics 2021Quote: ... and the protein of interest was purified by affinity chromatography on a Ni-NTA column (Qiagen, Hilden, Germany), using a TRIS-HCl 50 mM ...
-
bioRxiv - Immunology 2024Quote: ... tumor protein extracts were generated from snap-frozen biopsies using a combination of TissueLyser LT (Qiagen, Hilden, Germany) and four rounds of freeze/thaw cycling ...
-
bioRxiv - Microbiology 2024Quote: DNA was extracted from samples using Qiagen’s AllPrep Bacterial DNA/RNA/Protein Kit (Cat# 47054; QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... sdAb and proteins from bacterial cell lysates (SLP and TXA1) were purified with Ni-NTA resin (Qiagen: 30210), using Tris Buffered Saline (TBS ...
-
bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
-
bioRxiv - Microbiology 2023Quote: ... The N-terminal His-tagged Sif protein (Lmo0946-His6) was expressed and purified using Ni-NTA resin (Qiagen) according to the manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2022Quote: ... or 0.2 or 0.4 μL of the protein solution and 0.2 μL of a series of commercially available crystallization screens (Qiagen). Conditions that provided initial hits were optimized using bespoke screens prepared at the crystallization facility ...
-
bioRxiv - Immunology 2023Quote: DNA was extracted from samples using Qiagen’s AllPrep Bacterial DNA/RNA/Protein Kit (Cat# 47054; QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Enrichment analysis was performed both on the differentially expressed genes and proteins through the use of IPA (QIAGEN Inc. ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted using an imidazole gradient and subsequently loaded onto a 5 ml StrepTactin Superflow Cartridge (Qiagen) at a flow rate of 0.8 ml/min ...
-
bioRxiv - Neuroscience 2023Quote: ... Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat. Nr. 85300). Lysates were span down at 20.000 rcf for 20 minutes at 4°C to remove debris ...
-
bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
-
bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and and concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
-
bioRxiv - Microbiology 2021Quote: ... Cln1-His6 and its associated proteins were co-purified following the Ni-NTA Spin Kit procedure (Qiagen, Valencia, CA). Cell lysate from the CLN1-His6 strain was used as a negative control ...
-
bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
-
bioRxiv - Neuroscience 2020Quote: The genes encoding protein groups significantly altered in epilepsy and control were analyzed using Ingenuity Pathway Analysis (IPA, Qiagen). We analyzed all differentially expressed proteins in the hippocampus (n=777) ...
-
bioRxiv - Microbiology 2021Quote: ... and H2A.Z (fused with a maltose-binding protein -MBP) purification were performed by affinity chromatography using Ni-NTA agarose (Qiagen) and amylose resin (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... The cell lysate was clarified by centrifugation at 13,000 rpm and protein was purified using Ni- NTA superflow resin (Qiagen), according to the manufacturer’s recommendations for a centrifugation-based protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Headless HA stalk proteins were expressed in 293F cells and purified using nickel-nitrilotriacetic acid agarose (no. 1018244, Qiagen) in 5-ml polypropylene columns (no ...
-
bioRxiv - Biochemistry 2021Quote: ... was transformed with an N-terminally His-tagged fusion protein of cytHPPK/DHPS (At1g69190) in the vector pQE30 (Qiagen). Overnight cultures grown at 30°C in LB broth supplemented with 35 µg/mL kanamycin and 100 µg/mL ampicillin were sub-cultured to an OD600 of 0.6 - 0.7 and induced overnight at 16°C with 100 mM isopropyl β-D-1-thiogalactopyranoside ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
-
bioRxiv - Microbiology 2022Quote: ... Tissue lysate was further used for DNA extraction with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany). Total DNA extraction of seawater filters was done using the entire filter ...
-
bioRxiv - Microbiology 2022Quote: ... N-terminal His-tagged SlPR1 and SlChi3 proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
-
bioRxiv - Microbiology 2020Quote: ... and 8 M urea [pH 8.0]) and His-tagged Tax proteins were precipitated with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen). After washing in buffer C (100 mM NaH2PO4 ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
-
bioRxiv - Synthetic Biology 2021Quote: ... After spinning down the insolubilized protein fraction the supernatant was loaded onto an equilibrated mini NiNTA spin column (Qiagen) and the flow-through was reloaded twice to maximize protein binding ...
-
bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
-
bioRxiv - Genomics 2022Quote: ... DNA and RNA was extracted from fresh-frozen tissue samples using the AllPrep DNA/RNA/Protein minikit (Qiagen, USA) as per the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... Recombinant SlERF.D7-6XHIS protein was purified by Ni2+gravity flow chromatography according to the manufactureer’s protocol (Ni-NTA Agarose, Qiagen) as described ...