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Citations for Qiagen :
801 - 850 of 1020 citations for Recombinant Human TGFB2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and the fusion proteins purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... and the supernatant was used for bacterial protein purification using Ni-NTA affinity resin (Qiagen: 30210).
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bioRxiv - Immunology 2024Quote: ... Proteins and complexes were purified from the cell supernatants using Nickel-NTA affinity resin (Qiagen: 30210), followed by Superose 6 (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... 1% v/v sodium dodecyl-sulfate pH 8.0) for protein extraction or in RLT buffer (Qiagen) for RNA extraction ...
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bioRxiv - Biochemistry 2024Quote: ... WT and mutant proteins were initially purified using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAgen). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... the ViiA 7™ Real-Time PCR system was used to run the Human Stem Cell RT² Profiler™ PCR Array (Qiagen, Hilden, Germany). For the analysis of cardiac differentiation-associated genes ...
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bioRxiv - Evolutionary Biology 2021Quote: Protein sequence alignments and tree production were made using the CLC main work bench (ver. 5.5.1, Qiagen) software package ...
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bioRxiv - Physiology 2019Quote: ... RNA was extracted using the AllPrep DNA/RNA/Protein Mini Kit according to manufacturer’s instructions (Qiagen, 80004). RNA quality was determined by Agilent 2200 TapeStation and quantified by Qubit (Life Technologies) ...
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bioRxiv - Plant Biology 2019Quote: Sitting-drop vapour-diffusion experiments identified suitable crystallisation conditions for native protein from the PACT screen (QIAGEN), with optimisation identifying the best conditions for crystal formation as 6 mg/mL protein combined 1:1 with 8% PEG 1500 ...
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bioRxiv - Microbiology 2019Quote: ... The DNA from the appendix samples was obtained using the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN), before the DNA extraction ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... and His-tagged protein was purified from cell lysate using a Ni-NTA spin column (Qiagen, 31014). The resulting product was run on a 12% Bis-Tris PAGE gel (Thermofisher ...
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bioRxiv - Molecular Biology 2020Quote: Nme2Cas9 protein was expressed in Rosetta (DE3) cells and purified using a Ni2+-NTA agarose column (QIAGEN) as previously described24 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 mM TCEP) the expressed protein was extracted from the supernatant using NiNTA resin (QIAGEN, Germantown, MD). The resulting eluate from the NiNTA resin using lysis buffer supplemented with 250 mM Imidazole pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
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bioRxiv - Plant Biology 2021Quote: Alignments of histone DNA and protein sequences was performed using CLC Main Workbench software version 8.1 (QIAGEN). Predictions of intrinsic disorder were undertaken by PONDR (58) ...
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bioRxiv - Immunology 2022Quote: ... https://www.beiresources.org/).Protein was purified using gravity flow purification with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen, Germany) and concentrated and buffer exchanged in Amicon centrifugal units (EMD Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... Expressed protein was purified by elution from a nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Germantown, MD) column with 0.5 M imidazole and buffer exchanged into TEV buffer (25 mM NaHPO4 ...
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bioRxiv - Genomics 2021Quote: ... RNA-protein complexes were then incubated with Ni-NTA sepharose (Qiagen, 100 μl of slurry per sample) overnight at 4°C and extensively washed ...
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bioRxiv - Developmental Biology 2020Quote: ... The significantly changed proteins were used as inputs for Ingenuity Pathway Analysis (IPA, Version 03-13, Qiagen). The IPA predicted the altered pathways ...
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bioRxiv - Biochemistry 2021Quote: ... All the protein samples were expressed in BL21(DE3) and purified using Ni-NTA Sepharose column (QIAGEN) and Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA were isolated from equivalent amounts of HDL total protein using the miRNeasy Mini Kit (Qiagen). For sRNA analyses ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... 0.2 µg of AFP1-His proteins was incubated with 20 µl Ni-NTA agarose beads (Qiagen, Germany) in buffer (25 mM Tris-Cl ...
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bioRxiv - Immunology 2020Quote: ... HIS-tagged DR protein monomers were produced by Hi5 insect cells and purified using Ni-NTA (Qiagen) and size exclusion columns (AKTA ...
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bioRxiv - Microbiology 2022Quote: ... and RNA was isolated using the All prep bacterial DNA/RNA/protein kit following the manufacturer’s (Qiagen) protocol ...
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bioRxiv - Microbiology 2022Quote: ... The biomolecular extraction was then performed using the commercial AllPrep DNA/RNA/Protein Mini Kit (Qiagen-80004), conducted on a customised robotic system owned by the lab (Tecan-LU_UNILU_EWS_EXTRACTION_EU-0908-Freedom EVO 200) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were further purified by reverse immobilized metal ion affinity chromatography (IMAC) using TALON resin (Qiagen), to remove uncleaved protein ...
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bioRxiv - Microbiology 2023Quote: Protein complexes were produced from the relevant pQE70 plasmid (see Table S2) in strain M15 pREP4 (Qiagen) as follows ...
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bioRxiv - Microbiology 2023Quote: ... Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen, Germantown, Maryland). Detection of proteins via Western blotting was performed by fluorescence detection using IR-Dye®-labeled fluorescent secondary antibodies and imaged using the Odyssey CLx Imager (LICOR Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Protein exaction from the deparaffinised sample was carried out using the Qiagen Qproteome kit (Qiagen, Hilden, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... CBS proteins with a TEV cleavable N-terminal His-tag were purified using Ni-NTA agarose (Qiagen) resin and were treated to gel filtration using a Superose 6 Increase 16/600 column or Superdex 200 Hiload 16/600 column (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from all the cell lines using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). The total RNA was then treated with Turbo DNA-free kit (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: Melting curves of proteins were measured using the Rotor-Gene Q real-time PCR cycler (Qiagen, Germany). 25 μl samples with a protein concentration of approximately 0.5 mg/ml in a buffer containing 100 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA for each Klebsiella strain was extracted using the AllPrep Bacterial DNA/RNA/Protein kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested and His-tagged tIgM-Fc (tFcµ) protein was purified using Ni-NTA Agarose (Qiagen) and subsequently ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from FACS-purified cells using Allprep DNA/RNA/protein mini kits from Qiagen. Sperm cells were lysed using differential extraction ...
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bioRxiv - Biophysics 2023Quote: ... 0.2 mM TCEP) the expressed protein was extracted from the supernatant using NiNTA resin (QIAGEN, Germantown, MD). The resulting eluate from the NiNTA resin using lysis buffer supplemented with 250 mM Imidazole pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction was used for the purification of heterologous proteins using Ni-NTA agarose resin (QIAGEN), previously equilibrated with equilibration buffer (200 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... The identified proteins were then subjected to annotate the biological functions by Ingenuity Pathway Analysis (IPA, Qiagen). Molecules from the dataset that met the cutoff of the Ingenuity Knowledge Base were considered for the analysis ...
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bioRxiv - Microbiology 2024Quote: ... His-tagged proteins were detected with a mouse anti-His HRP-conjugated antibody (1:3000) from Qiagen. MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Cell Biology 2020Quote: ... The relative expression of mRNAs of c-Myc regulated genes identified in RT2 Profiler™ PCR Array Human MYC Targets (Qiagen, Cat. no. PAHS-177Z) was determined by real-time quantitative RT-PCR ...
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bioRxiv - Biophysics 2020Quote: ... proteins were expressed in Escherichia coli strain BL21 (DE3) and purified via nickel-nitrilotriacetic acid affinity chromatography (Qiagen) followed by ion exchange chromatography on an Äkta system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The expressed EcoCas3-tag fusion proteins were purified using nickel affinity resin (Ni-NTA, Qiagen, Hilden, Düsseldorf, Germany). To remove tags ...
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bioRxiv - Molecular Biology 2019Quote: ... coli cells grown at 37°C by addition of 0.2 mM IPTG and native protein purified using Nickel affinity chromatography according to the manufacturer’s protocol (Qiagen). For N-terminal His-tag removal ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were purified from the cell lysate by affinity chromatography using a Nickel-NTA column (Qiagen, Valencia, CA). A solution containing 20mM HEPES pH7.2 ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... DNA and RNA were extracted using the Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany; #80004). The standard manufacturer’s protocol was followed to extract DNA and RNA from each cell pellet ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from dissected tissues using an AllPrep DNA/RNA/Protein Mini kit (Qiagen, Valencia, CA). The concentration and purity were determined using a 2100 Bioanalyzer (Agilent Technologies ...