Labshake search
Citations for Qiagen :
851 - 900 of 1864 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... The Klenow polymerization reactions were incubated at 37 °C for 1 h and then purified on ion-exchange cartridges (cat. no. 28306; Qiagen). The 194 purified reactions were dissolved in 50 µL of water each and pooled to generate the 3,880 members library (DEL3880 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Library plasmid pools were propagated in liquid culture in LB with ampicillin (100 μg/ml) at 30 °C overnight and extracted (Qiagen).
-
bioRxiv - Cancer Biology 2022Quote: c-miR isolations from blood serum were carried out using affinity column-based miRNeasy Serum/Plasma Advanced Kit (217204, Qiagen) according to the manufacturer’s instructions ...
-
Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The samples were powderized with a mortar and pestle under liquid nitrogen or a TissueLyser II chilled to -80°C (Qiagen). The samples were lysed in RIPA buffer for 30 min on ice with intermittent vortexing (0.15M NaCl ...
-
bioRxiv - Cell Biology 2019Quote: ... the manufacturer’s directions were followed with the exception of adding 500ul of 10% SDS (w/w) to the homogenate at 60°C for 20 minutes before the addition of P2 buffer (Qiagen). This allowed the brain tissue to better solubilize with the Qiagen buffers ...
-
bioRxiv - Genomics 2020Quote: ... We incubated our transposition reaction for 30 mins at 37°C and further extracted the ATACSeq DNA library using PCR purification kit (Qiagen) into 20⍰L elution volume ...
-
bioRxiv - Genomics 2021Quote: ... The membrane and adhering tissue were peeled off the caps using a razor blade and the peeled membrane was incubated in proteinase K digestion reaction overnight for 16 h at 56°C to maximize DNA yield after cell lysis The DNA was extracted using the QIAamp DNA Micro Kit (Qiagen) and the elution was done in 20 µL ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were then washed and reverse cross-linked at 65°C overnight and DNA was purified using a PCR purification kit (QIAGEN). After DNA purification ...
-
bioRxiv - Microbiology 2020Quote: ... Tissue sections were incubated with proteinase K for 18h at 56°C prior to extraction on the QiaSymphony (Qiagen, USA) following manufacturer recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... cultures were grown on R2A medium for 24 hours at 30°C and genomic DNA was extracted with the DNeasy Blood and Tissue DNA Kit (Qiagen) with pre-treatment for Gram-positive bacteria ...
-
bioRxiv - Cancer Biology 2021Quote: ... Eluted pellets were de-crosslinked at 65°C overnight and purified in 50 μl Tris-EDTA buffer using the QIAquick PCR Purification Kit (Qiagen). Differences in the DNA content from every immunoprecipitation assay were determined by real-time PCR using the ABI 7700 sequence detection system and SYBR Green master mix protocol (Applied Biosystems) ...
-
bioRxiv - Microbiology 2022Quote: ... with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen). RNA was harvested from three independent cultures as biological replicates per experiment ...
-
bioRxiv - Genetics 2022Quote: Total RNA was extracted from the stomach and pyloric caeca tissue samples stored at -80 °C (n = 4 for control and fly larvae, n = 5 for shrimp shell) using the RNeasy Plus Universal Kit (QIAGEN). RNA quality was assessed using a 2100 Bioanalyzer with the RNA 6000 nano kit (Agilent ...
-
bioRxiv - Biochemistry 2022Quote: ... Cell debris were removed by centrifugation (35,000 g at 4°C for 20 min) and the N-terminal His-tagged proteins were purified from the supernatant using NiNTA agarose (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells incubated for 24 hours in a 37 °C incubator and transfected with hsa-miR-24-3p miRCURY LNA miRNA Power Inhibitor (Qiagen, Germantown ...
-
bioRxiv - Cell Biology 2019Quote: ... The reaction was stopped using 15mM EDTA followed by incubation at 65°C for 5-7 min and purification on RNAeasy column (Qiagen). The purified RNA samples were processed for preparations of cDNA libraries using the TruSeq Stranded Total RNA Ribo-Zero H/M/R (Illumina ...
-
bioRxiv - Microbiology 2019Quote: ... all clinical isolates and the UCBPP-PA14 reference strain were cultivated at 37 °C in LB broth and harvested in RNAprotect (Qiagen) at OD600= 2 ...
-
bioRxiv - Evolutionary Biology 2019Quote: Coral tissue samples were snap frozen in liquid nitrogen and kept at −80° C before extraction using the DNeasy Blood & Tissue Kit (QIAGEN) with the following modifications ...
-
bioRxiv - Cell Biology 2020Quote: RNA was isolated from snap-frozen kidney tissues or SOX9+ REC stored at -80°C using an Rneasy Mini kit (QIAGEN) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were lysed by boiling (5 min 95°C) followed by bead beating (3mm beads, 30 Hz for 1 min) (TissueLyser II, Qiagen) and sonication bath (3×10 sec at 4°C ...
-
bioRxiv - Immunology 2019Quote: ... Plugs were incubated twice (2 h of incubation followed by an overnight incubation) at 50 °C with 167 μL of freshly prepared proteinase K (Qiagen) in 2.5 mL of lysis buffer (BioNano Genomics Inc. ...
-
bioRxiv - Molecular Biology 2019Quote: ... The reaction was incubated at 30 °C for 90 min before applying it to 50 µl of Ni-NTA agarose resin (Qiagen) pre-equilibrated in W25 buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Genomics 2019Quote: ... to avoid fragmentation of long DNA molecules, with overnight lysis at 50°C in lysis buffer (Bionano Genomics, USA) with Puregene Proteinase K (Qiagen). The volume ratio (v/v ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were pelleted at 350 x g for 5 min at 4 °C and lysed in 350 μl RLT buffer of the RNeasy Mini Kit (Qiagen). RNA was isolated according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 20,000 rpm for 1 hour at 4°C and the soluble fraction was affinity purified by gravity column with Ni-NTA affinity resin (QIAGEN). The protein was eluted by 50 mM Tris pH 8 ...
-
bioRxiv - Microbiology 2019Quote: ... Cell debris was removed by centrifugation at 20000 g for 40 min at 4 °C and the lysate incubated with Ni-NTA Agarose Beads (Qiagen). After washing ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were pelleted and frozen at -80°C until total cellular RNA extraction was performed using the RNeasy Protect Bacteria Mini Kit (Qiagen). Total RNA was processed ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
-
bioRxiv - Microbiology 2021Quote: ... The lysate was clarified by centrifugation at 10,000 × g for 30 mins at 4 °C and the supernatant was applied to an Ni-NTA affinity column according to the manufacturer’s instructions (Qiagen, Germany). Bound proteins were washed twice with wash buffer (50 mM NaH2PO4 ...
-
bioRxiv - Genomics 2021Quote: ... The reactions were then treated with RNaseI (Thermo) for 60 min at 37°C and cleaned using the MinElute PCR Purification Kit (Qiagen). Subsequently ...
-
bioRxiv - Plant Biology 2019Quote: ... C-terminal His-tagged proteins were purified by gravity-flow chromatography on a nickel nitrilotriacetate (Ni-NTA) agarose resin (Qiagen) according to the manufacturer’s recommendations followed by an exclusion chromatography on a Superdex75 column connected to an ÄKTA Purifier™ (GE Healthcare) ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysate was centrifuged for 10 min at 16,200xg 4°C and 600 μL of the supernatant was added to a 50 μL slurry of Ni-NTA Agarose (Qiagen) that had been washed three times with 250 μL A3B-CTD lysis buffer without 2-mercaptoethanol and once with 250 μL of A3B-CTD lysis buffer ...
-
bioRxiv - Molecular Biology 2019Quote: ... worms were homogenized in PBS (50 μl/adult worm pair or 50 μl/1000 schistosomula) at 4°C using a TissueLyser II (Qiagen), homogenates were incubated overnight with mixing at 4°C and the supernatants collected by centrifugation at 15,000 g for 1 h at 4°C ...
-
bioRxiv - Pathology 2019Quote: ... DNA from D&C and endometrial biopsy specimens was extracted using the QIAamp® DNA FFPE Tissue Kit (QIAGEN, USA). All DNA was quantitated using the Qubit 2.0 Fluorometer (ThermoFisher Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... Samples were incubated for 15 minutes at 37°C to methylate accessible chromatin before the reaction was stopped with the addition of RLT plus buffer (Qiagen) and samples frozen down and stored at -80°C prior to processing ...
-
bioRxiv - Genomics 2019Quote: ... Reactions were incubated for 30 min at 37° C in a thermomixer shaking at 300 rpm and DNA purified using Qiagen PCR clean-up MinElute kit (Qiagen). The transposed DNA was subsequently amplified in 50µl reactions with custom primers as described (Buenrostro et al. ...
-
bioRxiv - Microbiology 2019Quote: ... an 8 μg of the isolated RNA was further treated with 2U of TURBO DNase (Invitorgen) at 37°C for 1 h and purified with RNeasy spin columns (Qiagen). The amount of RNA was quantified with Qubit RNA BR assay kit (Invitrogen) ...
-
bioRxiv - Genomics 2021Quote: ... This was followed by RNase A treatment for 1 h at 37°C and purification using MinElute PCR purification columns (Qiagen). cDNA amplification was performed with reporter-specific nested cDNA primers (Oligos 16 and 17 for libraries in vectors d and e ...
-
bioRxiv - Genomics 2021Quote: ... To eliminate lipids supernatants were applied to a RNeasy column and centrifuged at 10,000 rpm and 4 °C for 1 min (Qiagen, 74104). The flow through was collected and protein concentrations were assessed using the Bradford assay ...
-
bioRxiv - Genetics 2020Quote: ... coli strains expressing (His)6-Clr4 and (His)6-Swi6 and allowed to bind at 4°C overnight to Ni-NTA resin (Qiagen; binding capacity 5-10mg protein/ml resin) ...
-
bioRxiv - Genetics 2021Quote: ... Swabs were stored without media at - 20°C until DNA was extracted with QiaAmp DNA Mini kit (Qiagen, Germantown, MD). Nasal shedding status of M ...
-
bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
-
bioRxiv - Developmental Biology 2020Quote: ... overnight incubation at 37 °C) and DNA was extracted using the QiaAmp Blood Mini Kit (Qiagen Cat No./ID: 51304) following manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... Confirmation and segregation of the HNRNPUL1 c.1406dup variant was performed by PCR amplification (HotStar Taq Plus, Qiagen, Toronto, ON) from genomic DNA and Sanger sequencing with the ABI BigDye Terminator Cycle Sequencing Kit v1.1 (Life Technologies ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... washed twice with ddH2O and digested at 55°C overnight with 600 μL ATL buffer from DNeasy Blood and Tissue Kit (Qiagen), 24 mAu proteinase K (Qiagen) ...
-
bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
-
bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 45 min after which 25 µl of proteinase K (20 mg/mL) and 200 µl Buffer AL (Qiagen) was added ...
-
Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Lysates were cleared by centrifugation at 15000 x g for 30 min at 4 °C and incubated in Pierce 5 ml columns with Ni-NTA agarose beads (Qiagen) at 4 °C for 1 h ...
-
bioRxiv - Immunology 2020Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cβ primer ...