Labshake search
Citations for Qiagen :
651 - 700 of 1943 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 0.6 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture (1 ml beads per 1 l expression culture was used in case of Drosophila BiP ...
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bioRxiv - Microbiology 2019Quote: ... at 37°C for 1 hour and cleaned up with the QIAquick PCR Purification Kit (Qiagen, Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 37 °C) with 50 μg RNA sample and purified using RNeasy Protect Mini Kit (catalog no. 74124, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL2-Rα ectodomain was produced with C-terminal 6xHis tag and purified on Nickel-NTA spin columns (Qiagen) according to manufacturer instructions.
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bioRxiv - Cancer Biology 2022Quote: ... for 24 hours at -20°C prior to DNA/RNA extraction with AllPrep DNA/RNA Mini Kit (Qiagen). Cryopreserved patient ascites cells and PDX cancer spheroids were thawed and rinsed in serum free RPMI before RNA/DNA extraction ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was eluted from the column using 5 mL of prewarmed (50°C) QF Buffer (Qiagen, Germany). DNA was precipitated by adding 0.7 volumes of isopropanol ...
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bioRxiv - Neuroscience 2022Quote: ... Reaction was incubated at 37 °C for 30 min and purified using the QIAGEN MinElute Kit (28004 QIAGEN) according to the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... Tregs were pelleted and stored at -80°C in RTL lysis buffer (RNeasy micro kit, Qiagen, Hilden, Germany) with β-mercapto-ethanol (1:100) ...
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bioRxiv - Genetics 2022Quote: Liver samples were stored frozen at −80 °C before RNA extraction with the RNeasy kit (QIAgen, Crawley, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was stored at −80°C until further clean-up using RNeasy Mini kit (Qiagen, catalog #74104) manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... One hour of tagmentation at 37°C was followed by DNA extraction using MinElute PCR Purification Kit (Qiagen). Extracted DNA was subjected to PCR amplification using unique primers sets (Nextera XT v2 Full set (N7-S5)) ...
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bioRxiv - Microbiology 2023Quote: ... homogenized samples were stored at −20 °C prior to extraction using the DNeasy® PowerSoil® Kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... at -20°C or immediately processed for RNA using the RNeasy Mini Kit as per manufacturer’s protocol (Qiagen). Real-time analyses were performed as previously described (Van Otterloo et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Genomics 2023Quote: ... or the buffy coat of the BALB/c strain using the QIAsymphony DNA Mini Kit (cat:931236, Qiagen). The extracted gDNA was sheared to 150 bp in size using a Covaris LE 220 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... 10 minutes 4°C and the supernatant was incubated with pre-equilibrated Ni-NTA agarose beads (Qiagen, #1018244) with shaking on ice for 3 hours ...
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bioRxiv - Biochemistry 2023Quote: ... for 45 min at 4°C and the supernatant was mixed with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated at 37 °C for 15 min and then quenched by adding RLT buffer (Qiagen). The RNA was then extracted using RNeasy kit (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA from the Swedish cohort was extracted from whole blood using Puregene Blood Core Kit C (Qiagen). Illumina whole-genome sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... the mouse anti-penta-His antibody (Qiagen; Catalog #34660) was used at a 1:2,000 dilution in 3% BSA in PBS with sodium azide ...
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bioRxiv - Biophysics 2019Quote: ... Biotinylated mouse anti-5xHis antibody was purchase from QIAGEN. Mouse anti Tubulin beta 3 antibody from BioRad (Hercules ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... or mouse anti-RGS-His antibody (Qiagen; cat # 34610) at 1:5000 dilution followed by goat anti-rabbit-HRP conjugate (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... mouse antibodies against the His-tag (1:1000, Qiagen), rabbit antibodies against the C-terminal region of the collagen VI α1 chain (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-penta-His antibody (34660; Lot# 136244018; Qiagen); mouse anti-Myc tag mAb ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...
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bioRxiv - Plant Biology 2022Quote: ... and NoFa-ICL was the Penta-His antibody (Qiagen) at 1:4000 in TBST ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems ...
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bioRxiv - Biophysics 2023Quote: ... Anti-Penta-His antibody was purchased from Qiagen (Germany).
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bioRxiv - Biophysics 2023Quote: ... The antibodies were anti-RGSHis (Qiagen, Cat. No. 34650) and peroxidase-conjugated anti-mouse antibody (Dako ...
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bioRxiv - Bioengineering 2020Quote: ... with 1% 2-Mercaptoethanol (Serva) and disrupted using the Qiagen Tissue Ruptor (Qiagen). Total RNA was extracted using the RNeasy Fibrous Tissue Mini Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2019Quote: ... where it was incubated with 2 mL] Ni-NTA agarose bead slurry (QIAGEN) for 10 min at 4 °C ...
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bioRxiv - Immunology 2019Quote: RNA was extracted from 2×105 H292 using the RNeasy Mini Kit (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... bron-1 and bron-2 root tips using the RNeasy Micro Kit (Qiagen). qPCR was performed with SYBR green (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The cell lysate was applied on 2 ml of Ni-NTA agarose (QIAGEN) equilibrated with Buffer A ...
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bioRxiv - Microbiology 2020Quote: ... Soluble ACE 2 was purified using Nickel -NTA agarose beads (Qiagen Cat.No-1018244), eluted with 200mM imidazole ...
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bioRxiv - Plant Biology 2020Quote: ... The extracted DNA was eluted in 2 x 100 μL EB buffer (Qiagen), cleaned and concentrated with the Clean and Concentrator-5 kit (Zymo Research ...
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bioRxiv - Biochemistry 2020Quote: ... coli and purified using Qiagen Ni+2 agarose as per vendor protocol (Qiagen). Recombinant proteins were dialyzed in demethylation buffer overnight at 4°C prior to use in enzymatic assays.
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bioRxiv - Microbiology 2020Quote: 2 □ 107 spores were mixed with 1 ml RNAlater RNA stabilization reagent (Qiagen) and centrifuged at 17,000 g for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... submerged for 2 hours in PaxGene fixation reagent at room temperature (Qiagen #765312), kept overnight at 4°C in PaxGene stabilization reagent ...
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bioRxiv - Pathology 2022Quote: ... with stainless steel beads (2×5 mm) using a TissueLyser (Qiagen, Germantown, MD) for three minutes at 25 r/s twice ...
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bioRxiv - Microbiology 2020Quote: ... with 2 × 5 mm stainless steel beads using the TissueLyser (Qiagen, Hilden, Germany) for 3 minutes at 25 r/s twice ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from 2 × 106 cells using QIAzol Lysis Reagent (Qiagen) and cDNA was generated from 0.5 μg of RNA with random hexamers using the GoScript kit (Promega ...
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bioRxiv - Pathology 2021Quote: Saos-2 cells RNA was extracted using RNeasy mini kit (Qiagen, Catalog#:74104). and gene expression was carried using the RT² Profiler PCR Array for human cellular stress responses (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... in a 2 mL centrifuge tube for 3 min using a TissueLyzer (Qiagen). The obtained mixture was serially diluted in 5.0 mL sterile phosphate buffer and 150 µL aliquots of 10−4 to 10−7 dilutions were spread onto plates containing a range of media ...
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bioRxiv - Genetics 2020Quote: ... with the addition of a homogenization step using a TissueLyser 2 homogenizer (Qiagen) with a 4-mm steel bead ...
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bioRxiv - Cell Biology 2020Quote: ... phosphatase inhibitors 2 and 3) and then passing through Qiashredder columns (79656, Qiagen). Tissue samples were weighted ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with 50 mM Tris-HCl ...