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Citations for Qiagen :
801 - 850 of 1826 citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Preparation of his-tagged recombinant proteins was performed according to manufacturer instructions (Qiagen). Preparation of GST-tagged recombinant proteins was performed according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... The protein was purified under non-denaturing conditions using Ni-NTA resin (Qiagen) at 4 °C according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Tissues were then lysed in protein isolation buffer using a tissue-lyser (Qiagen). Protein concentration was measured by Bradford assay ...
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bioRxiv - Biophysics 2023Quote: ... His6-MBP-HNRNPH2 proteins were first purified using Ni-NTA Agarose (#30230, Qiagen) and eluted with buffer containing 250 mM imidazole pH 7.8 (#79227 ...
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bioRxiv - Cancer Biology 2022Quote: ... EV and protein fractions using miRNeasy serum/plasma kit (Qiagen 1071073 Lot#160020206) after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035) ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Nickel sepharose according to the manufacturer’s instructions (QIAGEN). The purified recombinant protein was dialyzed overnight against dialysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by immobilized metal affinity chromatography using Ni–NTA agarose (Qiagen) pre-equilibrated with lysis buffer in individual Econo-Pac gravity-flow columns (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: SAINT Express protein hits were analyzed using Ingenuity Pathway Analysis (IPA) software (Qiagen). Significant hits were analyzed and enrichment z-score was calculated for different pathways ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant TDP-43 proteins were purified over Ni-NTA agarose beads (Qiagen) and eluted using 50 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... All nucleotide and protein sequences were analyzed using CLC Main Workbench 8.1.2 (Qiagen).
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bioRxiv - Bioengineering 2024Quote: ... Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen) and eluted with DPBS containing 150 mM imidazole.
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bioRxiv - Neuroscience 2023Quote: Protein pathway analysis was conducted on DEP using Ingenuity Pathway Analysis (IPA, Qiagen) and Gene Ontology (GO ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen; Table S4), eluted with 50 mM Tris-base ...
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bioRxiv - Molecular Biology 2021Quote: qPCR on cDNA and ChIP samples was carried out in reactions containing SYBR Green I HotStarTaq enzyme (Qiagen). Relative quantitation was performed using standard curves made by pooling aliquots of each cDNA sample being examined ...
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bioRxiv - Physiology 2019Quote: DNA was isolated from intestines containing fecal material using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol for purification of total DNA from animal tissues and for pretreatment of Gram-positive bacteria ...
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bioRxiv - Molecular Biology 2019Quote: ... The peak fractions containing Esco2 were pooled and applied onto a 1 ml Ni-NTA Superflow column (Qiagen), equilibrated with 20 mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... resuspended in 50 mM sodium citrate buffer containing 10 µl of RNase A (20 mg/ml, Qiagen 76254) and incubated for 2 hours at 50°C ...
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bioRxiv - Microbiology 2021Quote: ... RNA containing aqueous phase of 600 µL was collected from each sample and passed through Qiashredder column (Qiagen) at 21,000 x g for 2 min to homogenize any remaining genomic DNA in the aqueous phase ...
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bioRxiv - Neuroscience 2020Quote: ... containing Protease inhibitor cocktail (PIC, Complete, 1:25) and lysed at 4°C using a TissueLyser LT (Qiagen) on 50 Hz for 2 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was homogenized in 1 ml phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (Thermo) and 0.1% RNase A (Qiagen) using a BioMasher (Nippi ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0) containing 15 mg/mL of lysozyme + 15 µL of proteinase K solution (20 mg/mL, Qiagen), and then incubated for 8–10 min ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Cell Biology 2022Quote: ... the aqueous phase containing RNA was mixed with 70% ethanol and transferred to RNeasy MinElute spin columns (QIAGEN). The cleanup and further concentration of the RNA was done using the standard procedure of RNeasy MinElute Cleanup Kit.
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bioRxiv - Immunology 2022Quote: ... and 5 cells per well were sorted into a 96-well plate containing TCL buffer (Qiagen, cat. 1031576) with 1 % beta- Mercaptethanol and snap frozen on dry ice ...
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2022Quote: ... The upper aqueous phase containing RNA was carefully transferred to a 2 ml collection tube (cat. 990381, Qiagen) without touching the interphase and placed in a QIAcube instrument for extraction.RNA extraction was carried out using the recommended protocol (FIW-50-001-J_FW_MB and PLC program version FIW-50-002-G_PLC_MB ...
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bioRxiv - Microbiology 2021Quote: ... the cell lysate was loaded by gravity flow onto a column containing 1 ml Ni-NTA resin (Qiagen) equilibrated with buffer A ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The supernatant containing the DNA solution was added to an equilibrated Qiagen genomic tip 100 (Qiagen, Venlo, Netherlands). Genomic DNA was eluted and precipitated using isopropanol ...
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bioRxiv - Microbiology 2019Quote: ... The final concentrated solution was transferred to a microtube containing Pathogen lysis tube L beads (Qiagen, Hilden, Germany), 50 μl of Solution SL and 200 μl of sterile saline solution to homogenize ...
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bioRxiv - Molecular Biology 2021Quote: A plasmid containing the modified Widom 603–42 sequence31 was purified with a QIAquick PCR Purification Kit (Qiagen) and used as the template for the amplification.
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bioRxiv - Microbiology 2020Quote: ... RNA was purified from 280 μl VLP-containing supernatant using a QIAamp viral RNA (vRNA) Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Frozen samples of liver and gill tissue were placed together in 600μl of lysis buffer containing 0.5% foaming reagent (Reagent DX, Qiagen) and 1% of ß-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... and placed into 2ml eppendorf tubes containing 200μl LB and a stainless steel bead of 5mm diameter (Qiagen). Flies were homogenized using the TissueLyser II (Qiagen ...
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bioRxiv - Immunology 2019Quote: MDM sorted as described above in “Flow cytometry” were collected into tubes containing RLT buffer (Qiagen, Hilden, Germany) and RNA was isolated using RNeasy Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... PBS/G was replaced with PBS/G containing 0.2 mg/mL proteinase K (Qiagen N. V., Hilden, Germany) or various concentrations of sodium azide ...
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bioRxiv - Microbiology 2021Quote: Frozen samples of liver and gill tissue were placed together in 600μl of lysis buffer containing 0.5% foaming reagent (Reagent DX, Qiagen) and 1% of ß-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Drops were prepared by mixing 0.1 µl of protein solution with 0.1 µl of precipitant solution containing 0.2 M magnesium chloride and 20% PEG3350 (NeXtal PACT Suite, Qiagen). Crystals were harvested after 2 weeks ...
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bioRxiv - Cancer Biology 2022Quote: ... the aqueous phase containing RNA was mixed with 70% ethanol and transferred to RNeasy MinElute spin columns (QIAGEN). Cleanup and further concentration of the RNA was done using the standard procedure of RNeasy MinElute Cleanup Kit.
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from shoot apexes containing young leaves using RNeasy Plant Mini kit (QIAGEN, Hilden, Germany). RNAseq libraries were prepared using the Illumina TruSeq Stranded RNA LT kit (Illumina ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... 440 μL of MDA reaction mixture containing 290 μL REPLI-g sc Reaction Buffer (Qiagen, catalog no. 150345), 20 μL REPLI-g sc DNA Polymerase (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: All dsDNA was derived from purified plasmid DNA from bacterial cultures containing the indicated plasmid (Qiagen Plasmid Plus) or by SPRI purification of amplified linear dsDNA.
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bioRxiv - Microbiology 2022Quote: ... RNA containing aqueous phase was collected from each sample and passed through a Qiashredder column (Qiagen, Valencia, CA) at 21,000 x g for 2 min to homogenize any remaining genomic DNA in the aqueous phase ...
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bioRxiv - Physiology 2022Quote: ... approximately 100 mg of adipose tissue and a 7 mm metal bead were placed in a 2 mL centrifuge tube containing 0.5 mL PBS buffer (pH 7.4) and homogenised in TissueLyser II (Qiagen) for 2 minutes at 25 Hz ...
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bioRxiv - Immunology 2022Quote: ... 4h and 20h post activation 2000 cells were sorted directly into 0.2ml semi-skirted 96 well PCR plate (Peqlab) containing 10μl 1x TCL Buffer (Qiagen) and 1%(v v-1 ...
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bioRxiv - Microbiology 2023Quote: ... Matched samples containing total mtDNA were isolated from whole cell extracts using DNeasy Blood and Tissue kits (Qiagen). qPCR was performed on both the cytosolic fraction and whole cell extract for each experimental condition using mtDNA specific primers ...
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bioRxiv - Developmental Biology 2023Quote: Approximately 15 female flies of CantonS were dissected in 1XPBS before being transferred to tubes containing RNAlater (Qiagen) on ice ...
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bioRxiv - Microbiology 2023Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen). Primers ABR-RT-OF and ABR-RT-OR2 were used to sequence the 5’ and 3’ ends of reverse transcriptase ...
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bioRxiv - Developmental Biology 2023Quote: ... Plates were then placed at 4 0C before adding the reverse transcription mix containing 5’-biotinylated TSO (Qiagen). PCR products were cleaned up using 0.8:1 Ampure XP beads (Beckman Coulter ...