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Citations for Qiagen :
601 - 650 of 1911 citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris gels (Novex, Qiagen) using MOPS running buffer (Novex ...
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bioRxiv - Developmental Biology 2023Quote: ... After the protein digestion with Proteinase K (QIAGEN, Cat. No. #158920) and RNA digestion with RNase A (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... and AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen). Isolated DNA and RNA samples were quantified by Nanodrop (ThermoFischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen) and a gravity column (Biorad) ...
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bioRxiv - Biochemistry 2023Quote: ... The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...
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bioRxiv - Biophysics 2024Quote: SNAP-GAF protein was purified on Ni-NTA agarose (Qiagen 30210), followed by Amylose resin (NEB cat ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were purified using a two-column protocol: Ni-NTA (Qiagen) affinity chromatography followed by size exclusion chromatography (HiLoad Superdex 75 pg or 250 pg ...
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bioRxiv - Microbiology 2019Quote: ... and HRP-penta-His antibody (Qiagen) and HRP-anti-mouse IgG (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... The PentaHis antibody (Qiagen, Düsseldorf, DE) against His-Tagged proteins was diluted 1:5000 in TBS buffer containing 3% BSA and the membrane was incubated overnight at 4°C followed by 2×10 min washing steps in TBST and 1×10 min in TBS buffers ...
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bioRxiv - Biophysics 2023Quote: ... anti-Strep-tag antibody (Qiagen, 34850), or anti-HA-tag antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... A mouse anti-GST antibody (Qiagen) diluted (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Cell Biology 2019Quote: ... 20ng of total DNA was used in a qPCR reaction containing QuantiNova SYBR Green mix (Qiagen), which was ran on a StepOnePlus Real-Time platform (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid containing the cloned chitinase gene was isolated using the Qiaprep spin miniprep kit (Qiagen). Restriction digestion of the plasmid was done with XhoI and XmaI ...
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bioRxiv - Molecular Biology 2021Quote: ... the upper aqueous phase containing RNA was automatically purified with an RNeasy Plus Mini Kit (QIAGEN) on a QIAcube instrument (QIAGEN) ...
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bioRxiv - Neuroscience 2019Quote: All plasmids containing reporter transgenes were prepared using Qiagen Plasmid Mini Kit (Qiagen Ref No.12125). The plasmids were sent to Genetivision Corporation for injection ...
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bioRxiv - Genetics 2019Quote: ... for lysis in 2 ml safe-lock tubes containing one 5 mm stainless steel bead (Qiagen) for 2.5-3 minutes at 30 Hz using TissueLyzer II (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: Whole blood (10 μL) was collected in an RNase free Eppendorf tube containing lysis buffer (QIAGEN) and stored at −80°C until use ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted from regions containing mainly malignant cells using Allprep RNA/DNA kit from Qiagen.
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bioRxiv - Physiology 2021Quote: ... The upper phase containing RNA was further processed using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and proteinase K solution (20 mg/mL, Qiagen), followed by an incubation with vortex and lysis steps described in the kit ...
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bioRxiv - Cell Biology 2021Quote: The clarified lysate was then loaded onto an open column containing pre-equilibrated Ni-NTA (Qiagen). The column was washed with 20 mM Tris-HCl ...
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bioRxiv - Pathology 2022Quote: Total DNA containing nuclear DNA and mtDNA were isolated from LB using the DNeasy Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and 2 mg/mL of proteinase K (Qiagen), and incubated at RT for 10 min ...
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bioRxiv - Genomics 2022Quote: ... the supernatant containing the DNA was purified with Genomic-tip 100/G (Qiagen; Cat. no. 10243) with the Genomic DNA Buffer Set (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... DNA containing an internal etheno-dA modification was purified using a QIAquick PCR Purification Kit (Qiagen), processed by translesion DNA synthesis using a mixture of Sulfolobus DNA Polymerase IV and Vent (exo- ...
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bioRxiv - Neuroscience 2019Quote: ... Frozen tissues were placed into tubes containing a 5 mm stainless steel bead (Qiagen, Courtaboeuf, France) and 1 ml of Trizol reagent (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... Large quantities of plasmids containing verified inserts were obtained using the plasmid Maxi kit from Qiagen. The plasmids were linearized using NotI (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen) in Bionano Lysis Buffer according to the Bionano Prep Cell Culture DNA Isolation Protocol ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was extracted from the HD MSN-containing cultures using the RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality was quantified on the NanoDrop Spectrometer and 0.5 to 2 μg of RNA was synthesised to cDNA using the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... 2 mL cultures were immediately added to centrifuge tubes containing 4 mL RNAprotect Bacteria Reagent (Qiagen), vortexed for 5 seconds and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... RNA containing aqueous phase was combined with equal volume of RLT lysis buffer (Qiagen, Valencia, CA) and extracted using Qiagen AllPrep DNA/RNA 96-well system (Valencia ...
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bioRxiv - Biochemistry 2019Quote: ... The soluble fraction was loaded onto a column containing 5 ml Ni-NTA superflow resin (Qiagen), pre-equilibrated with buffer A ...
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bioRxiv - Microbiology 2019Quote: ... cytoplasmic and host membrane-containing cell fractions were obtained using the Qproteome Cell Compartment Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and supernatant was transferred to tubes containing 30µl precleared Ni-NTA agarose beads (Qiagen) in the presence of 0.05% Tween-20 and 15mM imidazole ...
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bioRxiv - Physiology 2020Quote: ... reverse transcription of total RNA containing miRs was performed with miScript II RT kit (Qiagen, UK) and specific primers for miR-26a ...
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bioRxiv - Genomics 2020Quote: E2.75 embryos were sorted into a preprepared lysis buffer containing 2.5 μL of RLT Plus (Qiagen) with 1 U/μL SUPERase-IN (Ambion) ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription of total RNA containing miRs was performed with miScript II RT kit (Qiagen, UK). qPCR was performed on a RotorGene™ 6000 (Corbett Research ...
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bioRxiv - Neuroscience 2023Quote: ... Aqueous phase containing RNA was precipitated using ethanol and then cleaned using the RNeasy kit (Qiagen). RNA was eluted in nuclease-free water ...
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bioRxiv - Physiology 2023Quote: ... Plasmids containing the ABG sequences from each individual were then mini prepped (27106X4, Qiagen, Hilden, Germany) and sanger sequenced with M13F and M13R primers (Azenta Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... The ethanol-containing mixture was spun in two rounds through the RNeasy Mini Spin column (Qiagen) at 10,000rpm for 1 minute each ...
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bioRxiv - Microbiology 2023Quote: ... the separated aqueous phase containing RNA was transferred to an RNeasy column (Qiagen, Venlo, The Netherlands) for purification followed by DNase I (Roche ...
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bioRxiv - Genomics 2023Quote: ... with each 20 μL reaction consisting of 1× QuantiFAST reaction mix containing ROX reference dye (Qiagen), 0.66 µM forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... Lysate supernatants were then passed through chromatography columns containing Ni-NTA agarose resin (reference: 30210; Qiagen). The bound 6x His-tagged toxins were eluted with elution buffer (50 mM NaH2PO4 ...
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bioRxiv - Developmental Biology 2023Quote: ... and placed in a well of 96-well plate containing 2,5µl of RLT plus buffer (Qiagen). At the end of cell collection ...
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bioRxiv - Immunology 2023Quote: ... RNA containing aqueous phase (600 μl) was collected and passed through QiaShredder column (Qiagen, Valencia, CA) at 21,000 x g for 2 minutes to homogenize any remaining genomic DNA in the aqueous phase ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from ALI cultured cells lysed with RLT buffer containing β- mercaptoethnaol (QIAGEN) and frozen at -80°C for downstream analysis ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the polyhistidine-tagged toxin was loaded onto a Ni-NTA agarose column (Qiagen). The column was then washed with a 20–120 mM gradient of imidazole to remove additional contaminating proteins ...