Labshake search
Citations for Qiagen :
801 - 850 of 1566 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The plasmid was purified using the QIAprep Spin Miniprep Kit (QIAGEN) and validated by sequencing ...
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bioRxiv - Microbiology 2022Quote: ... and the complementing plasmid extracted with QIAprep Spin Miniprep kits (Qiagen). The insert sequence was verified by Sanger sequencing (Functional Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids were purified on QIAprep Spin Miniprep columns (Qiagen, catalog # 27104) with an extra wash with PB binding buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We isolated plasmids from cells sorted into each bin (Qiagen, Germany) after overnight growth ...
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bioRxiv - Microbiology 2023Quote: ... Mutated plasmids were recovered using the QIAprep Spin Miniprep Kit (Qiagen) and sent for Sanger sequencing using universal M13 forward and reverse primers (Table S1) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were isolated with the Qiaprep Spin Miniprep kit (Qiagen, Germany). Primers were synthesized and purchased from IDT (USA) ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was isolated using the QIAprep spin miniprep kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The DNA handles were purified using HiSpeed Plasmid Maxi kit (Qiagen). For maleimide modification at the amine end ...
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bioRxiv - Immunology 2023Quote: ... Colonies containing the correct plasmid were further cultured and midipreps (Qiagen) were performed.
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bioRxiv - Genetics 2022Quote: ... PT5 cells were transfected with pMK33-SAM plasmid using Effectene (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... plasmid DNA was purified using the QIAquick PCR purification kit (Qiagen) and normalised to 250 ng/ul in nuclease-free water ...
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bioRxiv - Biochemistry 2023Quote: ... All the plasmids were purified using Qiagen miniprep kit (Qiagen 27104). For neon transfection of primary myoblasts ...
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bioRxiv - Cancer Biology 2023Quote: ... and plasmid DNA was extracted using an EndoFree Maxi Kit (QIAGEN).
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bioRxiv - Cancer Biology 2023Quote: ... 10 mL of Buffer S3 (From QIAGEN Plasmid Plus Midi Kit) was added to the mixture ...
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bioRxiv - Cancer Biology 2023Quote: Plasmid DNA preparations were generated using endotoxin-free MIDI kits (Qiagen). Large-scale AAV and Ad particle production was outsourced to Vector Biolab (10^13 IU/mL).
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bioRxiv - Cell Biology 2023Quote: ... The plasmid DNA was extracted using QIAprep Spin Miniprep Kit (QIAGEN), as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and isolated using the QIAGEN Plasmid Plus Midi Kit (QIAGEN 12943). All other plasmids for making AAV (capsid and helper ...
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bioRxiv - Microbiology 2023Quote: ... and plasmids were isolated using the Midi prep plus kit (Qiagen). pVSV-SARS-CoV-2-S with mCherry was constructed using the same method by replacing eGFP in the antigenome.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was extracted using QIAprep Spin Miniprep kit (Qiagen, Germany) or Nucleobond Xtra Midi (Macherey-Nagel ...
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bioRxiv - Microbiology 2024Quote: ... and the plasmids were purified using Ultra-Pure Miniprep kits (Qiagen) for use in transfections.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was purified using the QIAquick PCR Purification Kit (Qiagen). The resulting DNA was transformed into DH5κ E ...
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bioRxiv - Synthetic Biology 2024Quote: ... plasmid DNA was collected using multiple DNA Miniprep columns (Qiagen 27104) and pooled.
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was purified using a QIAprep Spin Miniprep Kit (QIAGEN) or a PureLink Quick Plasmid Miniprep Kit (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Plasmid was extracted by using the commercial kit from Qiagen (Germany), following manufacturers protocols.
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bioRxiv - Plant Biology 2024Quote: ... The plasmid DNA was extracted using QIAprep Spin Miniprep Kit (Qiagen) and the plasmid DNA was verified for the cloned PCR fragment with Sanger sequencing using the pJET_F and pJET_R primer set.
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bioRxiv - Cell Biology 2024Quote: ... which was performed using the manufacturer’s protocol (#12123, Qiagen Plasmid Minikit). Isolated plasmid was then sent for Sanger Sequencing to confirm the insertion of point mutations at the desired location and then used for further transfection experiments.
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bioRxiv - Microbiology 2023Quote: ... all cloning steps were performed in DH5α carrying plasmid pREP4 (Qiagen) for expression of the Lac repressor ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid DNA was isolated using the QIAprep Spin Miniprep Kit (Qiagen) and sequence verified by Elim Biopharm.
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bioRxiv - Microbiology 2024Quote: Plasmid DNA was amplified using REPLI-g® Midi Kit (QIAGEN), according to the manufactureŕs instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified using a QIAprep Spin Miniprep kit (Qiagen) or QIAGEN Plasmid Midi kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... The Endo-free plasmid maxi kit (#12162, Qiagen, Valencia, CA, USA) was used for plasmid purification.
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bioRxiv - Bioengineering 2024Quote: ... DNA for transfection was purified using EndoFree Plasmid Maxi Kit (Qiagen). For single plasmid transfections ...
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bioRxiv - Bioengineering 2024Quote: ... All plasmids were extracted using QIAprep® Spin Miniprep Kit (Qiagen). The gRNA09 cassette targeting pcaC was built via inverse PCR using back-to-back primers containing the 20-bp spacer ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were transfected in COS-7 cells with Effectene (Qiagen, Germany) according to the manufacturer’s protocols for 22 hours ...
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bioRxiv - Biochemistry 2023Quote: ... All constructs were purified using endotoxin-free plasmid extraction kits (Qiagen) and sequenced to verify intact reading frames.
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (QIAGEN) and constructs were validated by Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Immunology 2021Quote: ... were used for preamplification and qPCR arrays (Rat Antibacterial Response, PARN-148ZE, and Human Antibacterial Response, PAHS-148ZC; Qiagen) were used for the expression analysis of 84 genes involved in innate antibacterial responses in human and rat respectively ...
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bioRxiv - Biophysics 2019Quote: ... The cells were transiently transfected with cDNA encoding the rat TRPV2 and its mutants using the Effectene reagent (Qiagen) 48-72 hours before experiments ...
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bioRxiv - Neuroscience 2020Quote: RNA from rat Schwann cell cultures or mouse nerve tissue was extracted using an RNeasy Micro Extraction Kit (Qiagen). RNA quality and concentration was determined after extraction using a nanodrop 2000 machine (Thermo) ...
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bioRxiv - Immunology 2022Quote: ... TCRαβ+CD4-CD45RClow/- T cells from Il34+/+ and Il34-/- rats were extracted using RNeasy Mini Kit (QIAGEN, Hilden, Germany) and protocol of 3’ DGE RNA sequencing was performed as previously described (16) ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA was isolated from log-phase bacterial cells grown in the rat peritoneal cavity or in 2.5% NaCl HI broth using the RNeasy minikit (Qiagen). One microgram of purified RNA was converted into cDNA using QuantiTect® Reverse Transcription Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were prepared from 5 ml overnight culture using Miniprep kits (Qiagen) and the plasmids were sequenced using M13F-M13R primers (Table S4).
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bioRxiv - Cancer Biology 2021Quote: ... cultured overnight in liquid LB and extracted using Plasmid miniprep kit (Qiagen). Plasmid DNA sequences were checked using Sanger sequencing (Eton Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... pMSuperCos-ARS1 was isolated from DH5α using a Plasmid Maxi Kit (Qiagen). 100 ug plasmid were digested with 100 U Notl-HF and XbaI (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmid DNA was isolated from mutant clones using a standard protocol (QIAGEN). After paired-end Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The vectors were isolated using a plasmid maxi kit (Qiagen, Hilden, Germany) and transformed into E ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA was prepared using the QIAGEN Plasmid Plus Midi Kit (Qiagen 12943) for transfection grade DNA purification ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were then isolated using QIAprep Spin Miniprep (Qiagen, cat no 27104), sequenced and used as templates to generate PCR products ...
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bioRxiv - Genomics 2022Quote: ... the cultured bacteria was pooled prior plasmid purification (Qiagen, 12943 or 12963). Four of the aliquots were sampled and plated with serial dilutions after 1 hr recovery to estimate transformation efficiency.
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bioRxiv - Molecular Biology 2020Quote: ... Endotoxin-free plasmid DNA isolation kits were purchased from Qiagen (cat. 12362). Agarose ...