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Citations for Qiagen :
751 - 800 of 3702 citations for 7 PHENYL 1 2 4 TRIAZOLO 1 5 A PYRIMIDINE 6 CARBONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Quality control and de novo assembly of the reads were done using default parameters in CLC Genomics workbench 7 (Qiagen). Whole-genome alignment was performed using Mugsy v1.2.3 (55 ...
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bioRxiv - Immunology 2021Quote: ... single MR1-tetramer-binding cells from Peruvian participant 7-3 and blood bank donors 702A and 703A were sorted into 96-well plate coated with Vapor-Lock (Qiagen) containing Iscript cDNA synthesis mixture (Bio-Rad ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells with ESC like morphology were picked and 7 clones successfully expanded for genomic DNA isolation using DNeasy Blood and Tissue Kit (Qiagen) followed by genotyping using Taq DNA polymerase (Takara ...
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bioRxiv - Plant Biology 2021Quote: DNA for bisulfite sequencing was isolated from dissected endosperm at 7 days after pollination using QiaAMP DNA microkit (QIAGEN 56304). Dissected tissue was obtained for two biological replicates for each genotype and incubated overnight in a shaker at 56°C in ATL buffer with Proteinase K ...
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bioRxiv - Cell Biology 2021Quote: ... These M-cells were resuspended in 500 μl PBS pH 7.2 and mixed with 7 μl BioMag magnetic streptavidin beads (Qiagen, 311714) for 60 min at 4°C and loaded into an acrylic column with curved grade N52 magnet ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from freshly isolated T-cells on day 7 of treatment from spleens using RNeasy Kit (Qiagen). For each group ...
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bioRxiv - Pathology 2023Quote: ... Skeletal muscle (triceps and Tibialis anterior (TA)) and spinal cord tissue samples underwent homogenization with 7 mm stainless steel beads (#69990, Qiagen) in a Tissue Lyser LT (#85600 ...
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bioRxiv - Neuroscience 2023Quote: RNA was isolated from mature wildtype DA neurons following 7-day treatment with GluCer using the RNeasy Micro Kit (QIAGEN) and sent for bulk RNAseq to Azenta ...
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bioRxiv - Immunology 2024Quote: TET2 knockout efficiency was confirmed by isolating genomic DNA from CAR T-cells at Day 7 using the dNeasy Blood & Tissue Kit (Qiagen). PCR of genomic DNA was performed with TET2 Forward Primer 5’-TCCCTGAGTCCCAGTCCATC-3’ and Reverse Primer 5’-TCAGGAATGGCCAGGTTCTG-3’ using MyTaq Red 2X Mix (Meridian Bioscience) ...
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bioRxiv - Immunology 2023Quote: ... The resulting cell suspension was concentrated by centrifugation (7 min, 350 g) and lysed in RLT buffer(Qiagen, cat. 79216) 0.1-0.5 *10^6 lymphocytes /sample density ...
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bioRxiv - Plant Biology 2024Quote: ... about 20 mg of tissue from 7-day-old seedlings was ground into fine powder in liquid nitrogen with a TissueLyser system (QIAGEN). Total proteins were extracted using denaturing buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA from 7 of these patients was extracted from CD138+ cells using the miRNeasy Mini Kit (Qiagen GmbH, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... where the band corresponding to the desired handle length (∼1.6 kb or 1.7 kb for LH and RH respectively) was excised and then the DNA extracted using a gel-extraction kit (Qiagen). The RNA hairpin substrate was then annealed and ligated between the LH and RH handles (see final construct configuration as in Fig ...
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bioRxiv - Cell Biology 2021Quote: ... of healthy (4 weeks of age) and hTNFtg mice (4 & 8 weeks of age) using the RNeasy mini or micro kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: SUZ12 isogenic M3 MPNST cells were treated with or without DNMT inhibitors (50 nM daily Decitabine or 4 μM single dose of GSK862) for 4 days followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Bisulfite sequencing was conducted by the IGO core facility at MSKCC ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from infected (n = 4) and control (n = 4) bAM samples using the RNeasy Plus Mini Kit (Qiagen) as previously described [91] ...
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bioRxiv - Cell Biology 2023Quote: ... ULK2 (mixture of 4 siRNAs, GS9706), TBC1D14 (mixture of 4 siRNAs, GS57533) and non-targeting (NT, # 5091027310) were purchased from Qiagen.
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bioRxiv - Molecular Biology 2021Quote: ... or 6 µM and harvested 24 h later by adding RLT lysis buffer (Qiagen). Similarly ...
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bioRxiv - Neuroscience 2021Quote: ... tissue from 6 brains were pooled to prepare total RNA (RNEasy micro kit, Qiagen) for reverse transcription and amplification to cDNA (Ovation Pico WTA kit ...
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bioRxiv - Systems Biology 2020Quote: ... 3 mL of culture were mixed with 6 mL of RNAprotect bacteria reagent (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and harvested for RNA extraction after 6 hours of incubation using RNAeasy kits (Qiagen). cDNA was synthesized from 500 ng of RNA using Quantitect Reverse Transcriptase kits (Qiagen) ...
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bioRxiv - Genetics 2019Quote: HEK293Ts were plated in 6 well plates and transfected using Effectene Transfection Reagent (Qiagen) according the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... IL −6 and TNFα mRNA were detected by validated QuantiTect primer assays 144 (Qiagen).
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bioRxiv - Biophysics 2023Quote: TSA measurements were carried out on a Rotor-Gene Q 6 plex (Qiagen, Germany) instrument at a heating rate of 2 °C/min and a temperature range of 25−90 °C in the presence of a CPM dye ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM siRNAs were mixed with 6 µl of HiPerFect transfection reagent (Qiagen, #301707) in 100 µl of serum free DMEM and added to freshly plated cells drop by drop ...
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bioRxiv - Microbiology 2020Quote: ... respectively34 (Table 1) were analyzed on the Rotor-GeneQ machine (Qiagen) using the following PCR conditions ...
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bioRxiv - Cell Biology 2022Quote: To synthesize cDNA from 1 μg RNA the Quantitect kit (Qiagen) was used according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: RNA (1 µg) was extracted with the RNeasy Kit (QIAGEN, 74106) and treated with DNase I following the manufacturer’s instructions (QIAGEN ...
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bioRxiv - Developmental Biology 2019Quote: ... collected with PBS 1% SDS and passed through Qiashredder columns (Qiagen) to disrupt nucleic acids ...
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bioRxiv - Biophysics 2019Quote: ... and probed with primary antibodies anti-5His monoclonal (1:1,000; QIAGEN), anti-Myc monoclonal (1:1,000 ...
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bioRxiv - Genomics 2021Quote: ... 1% SDS) supplemented with 2µl of 0.5mg/ml RNase A (QIAGEN) at 55°C for 2 hours ...
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bioRxiv - Genomics 2019Quote: ... Each reaction contained 1× Rotor-Gene SYBR Green PCR Mastermix (Qiagen), 0.2 μM of primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or Junctional Adhesion Molecule 1 (JAM1) using Polyfect (Qiagen, CA, USA) and selected in 1000 µg/ml G418 for stable transfectants ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied to 1 ml Ni-NTA Agarose (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was decanted and 1 mL QIAzol Lysis Reagent (QIAGEN) was added to resuspend the cell pellet and protect the RNA ...
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bioRxiv - Microbiology 2020Quote: ... PCR#1 product was purified using Minielute PCR purification kit (Qiagen) and a second round of PCR amplification was performed with gene-specific primers R2 and forward F2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteinase digestion was performed with 1 mg/mL proteinase K (Qiagen) in the presence or absence of 1% Triton X-100 for 30 min at 37 °C as reported previously64 ...
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bioRxiv - Cell Biology 2022Quote: ... added to 1 ml of washed Ni2+-NTA agarose (Qiagen, #1018244) and rotated on a low speed wheel for at least 1h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Homogenization was performed after adding 1 ml of inhibitex buffer (Qiagen) using the Bead Ruptor Elite bead mill homogenizer (Omni ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml binding buffer (provided in Qiagen PCR purification kit, #28106) and 20 µl of 3 M sodium acetate pH 5.5 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was added to 1 mL Ni2+-NTA resin (Qiagen) and incubated for 1 hr at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... Lysates were incubated with 1 ml of Ni-NTA resin (QIAGEN) in 4°C for 1 hr ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatant was applied to 0.5-1 mL Ni-NTA resin (Qiagen) in gravity columns pre-equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... sDectin-1 was bound to a nickel affinity resin (QiaGen, #30210) in this same buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse transcription to cDNA was performed using 1 µg RNA (Qiagen QuantiTect Reverse Transcription kit ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was loaded on two 1-ml Ni-NTA (Qiagen 30210) columns ...
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bioRxiv - Microbiology 2019Quote: ... from 1 x 107 cells using the RNeasy Mini Kit (Qiagen) and the TruSeq Stranded mRNA kit (Illumina ...
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bioRxiv - Physiology 2021Quote: ... and 1% β-mercaptoethanol with a TissueLyser II (Qiagen, Manchester, UK) at maximum speed for 2 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... then diluted to 1 × 109 with 180 μL Buffer AE (Qiagen). Standard curves were prepared from 1 × 107 copies/rxn to 1 × 101 copies/rxn ...
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bioRxiv - Plant Biology 2022Quote: ... the PCR products were ligated into expression vector pQE-1 (Qiagen) with T4 ligase (New England BioLabs) ...