Labshake search
Citations for Qiagen :
751 - 800 of 1933 citations for 6H Pyrano 3 2 g benzothiazole 8CI 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from control and GDC-RPC-3 cells using the RNeasy PlusMini Kit (Qiagen). High-quality (Agilent Bioanalyzer RIN>7.0 ...
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bioRxiv - Microbiology 2022Quote: ... The soluble extracts were mixed for 30 min with 3 ml of Ni2+-NTA-agarose (Qiagen) that had been equilibrated with buffer A containing 10 mM imidazole ...
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bioRxiv - Genetics 2022Quote: ... Midi, 3-4 ml of blood) and Puregene (0.3-1 ml of blood, manual extraction) (Qiagen, Cat# 1057048 ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 million cells were seeded and transfected with 1μg pMK33-based plasmid DNA using Effectene (Qiagen). After 48 hours ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA (n=3/experimental group) was extracted from macrophages with RNeasy Mini Kit (Qiagen, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from 3 million transfected cells using the All Prep DNA/RNA kit (Qiagen) and quantified by Nanodrop 1000 ...
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bioRxiv - Microbiology 2020Quote: The 3 Kb mutagenic DNA fragments were purified using a QIAquick PCR Purification Kit (Qiagen; 28106) and transformed into V ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 μL water and 5 μL 2x QuantiFast® SYBR® Green PCR Master Mix (Qiagen). Technical duplicates of this reaction mix were then analyzed on a Corbett Rotor-Gene 6000 real-time PCR cycler ...
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bioRxiv - Biochemistry 2020Quote: ... and pJET_Luc_FL_UTR_REV (5’-GCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAAATGC-3’) primers and was purified with the QIAquick PCR Purification Kit (Qiagen) directly after the PCR reaction ...
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bioRxiv - Genetics 2021Quote: RNA was extracted from an aliquot of 3 × 106 cells using the RNeasy Plus Kit (Qiagen). cDNA was synthesized from extracted RNA using the iScript cDNA Synthesis Kit (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... Media and osteoblasts were harvested 3 days later and EVs were collected using Exoeasy kit (Qiagen). Total RNA was extracted using miRNeasy micro (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: ... RNA from 3 x 106 HMC-1.2 cells was extracted using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 3-month-old zebrafish (1 fish/sample) using the RNeasy Mini Kit (Cat. No. 74104; Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was isolated (n = 3 or 4 mice per sample) using the RNeasy Mini Kit (QIAGEN), and aRNA was synthesized with Ambion’s MessageAmp aRNA Kit (Catalog # 1750 ...
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bioRxiv - Cell Biology 2022Quote: ... A Locked Nucleic Acid (LNA) probe was used to detect centromere regions (5’-TTGGCTACACCATGAAAGCTT-3’; Qiagen). 20 μL of probe mix (250 nM LNA probe ...
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bioRxiv - Microbiology 2022Quote: ... 3 mL samples were added to tubes containing 6 mL RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) and vortexed ...
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bioRxiv - Genetics 2022Quote: ... 3 min in 37°C shaker) and processed with DNeasy Blood and Tissue Kit (QIAGEN #69504). Two hundred ng of genomic DNA was used as input in the DamID protocol that included an improved pool of AdR primers as described (de la Cruz Ruiz et al ...
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bioRxiv - Biochemistry 2023Quote: The total RNA was extracted from 3 × 106 HEK293T cells using the RNeasy Mini Kit (QIAGEN). RNA served as a template for subsequent reverse transcription into cDNA by iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... whole eyes were homogenized in PBS using a TissueLyser II (Qiagen, 30 Hz for 3 minutes), and homogenates were serially diluted and streaked on LB plates for quantification of colony forming units (CFU ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was added to 3 ml of Ni-nitrilotriacetic acid (NTA) superflow resin (Qiagen) and the mixture was applied onto a gravity drip column ...
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bioRxiv - Cancer Biology 2024Quote: ... and total RNA was extracted using the RNAeasy Mini kit (Qiagen, 74104; n = 3 independent experiments). Prior to library construction ...
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bioRxiv - Microbiology 2023Quote: ... Samples were mechanically disrupted for 2x 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). This was followed by adding 5 μl of proteinase K (20 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: 2 x 107 cells were harvested by centrifugation and genomic DNA was prepared using Blood & Cell Culture DNA Midi Kit 100/G Genomic-tips (Qiagen #13343). To examine alkaline degradation 3 μg of DNA was treated with 0.3 M NaOH at 55 °C for 2 hr in 15 μl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... (2015) which is a modified CetylTrimethylAmmoniumBromide (CTAB) protocol (Doyle and Doyle, 1990) combined with a MagAttract suspension G (QIAGEN, Hilden, Germany) purification step (Xin and Chen ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was purified in a 0:0.72 volumetric ratio of Agencourt AMPure XP beads and amplified using multiple displacement amplification with the REPLI-g Single Cell Kit (Qiagen #150345) to yield an average of 815ng amplified genomic DNA per clone.
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bioRxiv - Genomics 2020Quote: ... ~20 ng genomic DNA was amplified using a whole-genome amplification (WGA) kit according to the manufacturer’s instructions (Qiagen REPLI-g Mini Kit, Qiagen, Valencia, CA). The REPLI-g Kit is developed based on multiple displacement amplification (MDA) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then harvested by centrifugation (5 min, 10,000 × g) and immediately suspended in 1 mL bacterial RNA protect (Qiagen, Germantown, MD), followed by 5 min incubation at room temperature ...
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bioRxiv - Genetics 2020Quote: ... We purified the digested vector from a 0.8% agarose gel (1.2 g agarose Fisher #21-255-00GM) in 150 mL 1x TAE (Fisher #FERB49)) using a QiaQuick Gel Extraction Kit (Qiagen #28706) and quantified using a Qubit dsDNA HS Assay Kit.
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bioRxiv - Neuroscience 2020Quote: ... Whole genome amplification (WGA) was performed directly following FACS using the REPLI-g Advanced DNA Single Cell kit (Qiagen Cat#: 150365) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected by centrifugation (5 min at 500 x g) and RNA was isolated using RNeasy Mini Kit (RNeasy Mini Kit, Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA for sequencing was isolated with the the Qiagen 100/G kit according to the manufacturer’s description (Qiagen, Hilden, Germany) and library preparation and sequencing was done as described previously using Illumina Miseq sequencing (Illumina ...
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bioRxiv - Microbiology 2020Quote: Total bacterial DNA from approximately 0.25 g of colon contents was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions as described13 ...
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bioRxiv - Microbiology 2021Quote: ... Suspended fecal samples and swab samples were centrifuged at 4,000 x g for 15 min and viral RNA was extracted from each supernatant using the QIAamp Viral RNA Mini Kit (Qiagen, USA). Conventional RT-PCR was performed using the Invitrogen™ SuperScript™ IV First-Strand Synthesis System (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were centrifuged for 10 minutes at 4,300 x g and the cell pellet resuspended in the lysis buffer (RLT) from the RNeasy kit (Qiagen, Germantown, MD). Cells were then homogenized with sterile 0.1 mm glass beads (Biospec Products Inc. ...
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bioRxiv - Cell Biology 2021Quote: Cells debris was pelleted by centrifugation at 23,000 x g for 45 minutes and the supernatant loaded onto a 10 ml Ni2+-NTA column (Qiagen, Hilden, Germany). The column was washed with 600 ml buffer A and protein batch eluted with 100 ml buffer B (buffer B = buffer A supplemented with 290 mM imidazole).
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bioRxiv - Microbiology 2020Quote: ... total DNA from the sample (20 g) was extracted using the Qiagen DNeasy® PowerSoil® Pro Kit (Qiagen, Hilden, Germany) and the integrity of DNA was evaluated by gel electrophoresis ...
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bioRxiv - Neuroscience 2022Quote: 1 µg of RNA was used for cDNA synthesis using the QuantiTect Reverse Transcription Kit according to the manufacturers’ protocol (Qiagen 205313). Quantitative PCR was carried out on a RotorGeneQ (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... and cDNA generation of whole embryos were performed using the multiple displacement amplification (MDA) reaction (REPLI-g WTA Single Cell, Qiagen, Germany), according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... the tubes were centrifuged at 8600 × g for 30 seconds and the resulting supernatant was used for total RNA extraction using the miRNeasy Mini kit (Qiagen, Germany) as per the manufacturer’s recommendation.
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bioRxiv - Microbiology 2020Quote: ... were transferred into 0.2-mL PCR tubes and genome amplified using REPLI-g Advanced DNA Single Cell Kit (Qiagen Hilden, Germany). For the whole culture approach ...
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bioRxiv - Microbiology 2020Quote: ... bacterial cultures were centrifuged for 10 minutes at 4,300 x g and the bacterial cell pellet resuspended in the lysis buffer (RLT) from the RNeasy kit (Qiagen, Germantown, MD). Bacterial cells were then homogenized with sterile 0.1 mm glass beads (Biospec Products Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... Lysates were clarified by centrifugation at 30,000 g for 30 min and loaded on a Ni-NTA Superflow column (Qiagen, Netherlands, 30761). After washing with each 20 column volumes of lysis and wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2020Quote: DNA extraction was performed by mixing 0.2 g of each feces sample with 500 μL of G2 buffer (QIAGEN, Hilden, Germany) in an Eppendorf tube (Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Frozen muscle was pulverized in liquid nitrogen and genomic DNA was extracted using a Genomic Tip 100/G kit (Qiagen, USA). DNA quantity was measured using a NanoDrop ND-8000 spectrophotometer and a Qubit dsDNA BR Assay kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μg of RNA per sample was reverse transcribed using a complementary DNA (cDNA) synthesis kit (QuantiTect reverse transcription kit, Qiagen, Germany). Quantitative PCR (qPCR ...
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bioRxiv - Genomics 2022Quote: ... We extracted genomic DNA with standard CTAB method and purified it with QIAGEN Genomic-tip 20/g (Qiagen KK, Tokyo, Japan).
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bioRxiv - Genetics 2022Quote: ... The nuclei were sorted into individual tubes and subject to single nucleus whole genome amplification (snWGA) using the REPLI-g® UltraFast Mini kit (Qiagen). The snWGA was performed by a multiple displacement amplification as in this previous publication (85) ...
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bioRxiv - Molecular Biology 2023Quote: DNA from ten (~0.25 g each) pieces of store-bought chicken breast was extracted using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Samples were thawed on ice and 6-10 aliquots of approximately 0.25 g each were subjected to DNA extraction using the PowerLyzer® PowerSoil® DNA Isolation kit (QIAGEN) following the manufactureŕs instructions ...
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bioRxiv - Zoology 2023Quote: A portion of the total cvcDNA preparation was subjected to rolling circle amplification (RCA) by using a Repli-G mini kit (Qiagen, Germany) following the manufacturer’s protocol ...