Labshake search
Citations for Qiagen :
651 - 700 of 1933 citations for 6H Pyrano 3 2 g benzothiazole 8CI 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 30% or fewer HIV-1 DNA positive wellsSorted pools were then subjected to whole genome amplification by multiple displacement amplification (MDA) using the Advance Single Cell Repli-G kit (Qiagen), following manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... the beads were suspended in Buffer D2 and subjected to multiple displacement amplification using a REPLI-g Single Cell Kit (QIAGEN).
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bioRxiv - Microbiology 2021Quote: ... the beads were suspended in Buffer D2 and subjected to multiple displacement amplification (MDA) using a REPLI-g Single Cell Kit (QIAGEN).
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bioRxiv - Microbiology 2021Quote: High-molecular weight (HMW) genomic DNA was isolated from liquid cultures using the Qiagen Genomic-tip 20/G kit (Qiagen). DNA was then subjected to a hybrid approach of both short- and long-read DNA sequencing to achieve high-confidence complete de novo genome assemblies ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was extracted from 0.25 g of an ethanol-free sample in duplicates using the DNeasy PowerSoil kit (QIAGEN, RRID:SCR_008539). The extraction protocol followed the manufacturer’s guidelines with several modifications (as described below) ...
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bioRxiv - Microbiology 2022Quote: ... for Illumina sequencing most samples were subjected to whole-genome amplification (WGA) with the REPLI-g Single Cell Kit (Qiagen), either directly from few ciliate host cells (four samples ...
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bioRxiv - Biophysics 2022Quote: ... Insoluble material was pelleted for 1 hr at 100,000 x g and the supernatant was added to Ni-NTA resin (QIAGEN, 30410) in batch and was eluted with an imidazole gradient containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... reaction was conducted after pressure ejection of the cytoplasm into a microcentrifuge tube containing the REPLI-g WTA single cell kit (Qiagen). Each cell was incubated in a total volume of 5.5 μl at 24°C for 5 min and then cooled to 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Individual single Giardia cysts or pools of 10 cysts isolated by FACS were subjected to MDA with the REPLI-g single cell kit (Qiagen). MDA was performed according to the manual ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was centrifuged (20 min, 27000 g) and the supernatant was applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers containing increasing concentrations of imidazole (20-50 mM ...
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bioRxiv - Zoology 2021Quote: Frozen single cells in PCR tubes were thawed and subjected to lysis and multiple displacement amplification (MDA) using the REPLI-g UltraFast Mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pelleted at 350 x g for 5 min at 4 °C and lysed in 350 μl RLT buffer of the RNeasy Mini Kit (Qiagen). RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris was removed by centrifugation at 20000 g for 40 min at 4 °C and the lysate incubated with Ni-NTA Agarose Beads (Qiagen). After washing ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes containing the channels were collected by centrifugation (100,000×g) and proteins were extracted with 40 mM DM.65 Channels were purified using Ni-NTA agarose beads (Qiagen). Liposome reconstitution was performed using E ...
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bioRxiv - Immunology 2021Quote: ... Cells were then centrifuged at 300 x g for 10 minutes and vigorously resuspended in 350µL RLT Buffer (Qiagen, Germany) by vortexing for 30 seconds ...
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bioRxiv - Immunology 2020Quote: ... the crude reaction mixtures were centrifuged for 10 min at 21,500 x g to remove the insoluble fraction before loading onto a chelated-Ni resin (Ni-NTA Agarose, Qiagen, Germany). Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted from 0.04-0.05 g of stored fecal material using QIAamp Fast DNA stool Mini kit (Qiagen, Hilden, Germany) as previously described (54) ...
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was isolated from 2 clones of each strain corresponding to 50 OD600 units with the Genomic DNA Buffer Set and Genomic-tip 100/G (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... total environmental DNA was extracted from approximately 5 g of sediment subsampled from inner part of WRC using DNeasy PowerMax Soil Kit (Qiagen) with a minor modification 79 ...
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bioRxiv - Microbiology 2021Quote: The cells of each positive clone were collected by centrifugation (400 × g for 10 min at room temperature) and the RNA extracted with the commercial kit RNeasy Mini (Qiagen), following the protocol established by the manufacturer ...
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bioRxiv - Genomics 2021Quote: Strains were grown overnight in 50 ml YPD broth and genomic DNA was extracted from approximately 4×109 cells using a QIAGEN Genomic-tip 100/G kit (10223, QIAGEN) with minor modifications ...
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bioRxiv - Plant Biology 2021Quote: ... Total DNA and RNA were extracted from ∼1.5 g of rhizosphere soil using the RNA PowerSoil Kit with the DNA elution kit (Qiagen, Germany). DNA and RNA were extracted using ∼0.03 g of roots using the DNeasy Plant DNA Extraction Kit ...
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bioRxiv - Cell Biology 2020Quote: ... samples were centrifuged for 10 minutes at 20000 g at RT and pellets obtained pellets were resuspended in 10 μl of EB (Qiagen) shaking at 55°C for 10 minutes and finally purified with a PCR purification kit ...
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bioRxiv - Microbiology 2020Quote: ... DNA for metagenomics analysis was isolated from 20 g (wet weight) sediment per sample with the PowerSoil DNA Isolation Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Whole genome amplification (WGA) was carried out on PicoPure-extracted DNA using Multiple Displacement Amplification43 (MDA) with the REPLI-g WGA kit (Qiagen) and Multiple Annealing and Looping-Based Amplification Cycles44 (MALBAC ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from 110 mutants plus the ancestral strain was extracted with the Genomic-tip 20/G Kit (Cat. No. 10223, Qiagen) according to the manufacturer’s instructions ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Lysates were cleared by centrifugation at 15000 x g for 30 min at 4 °C and incubated in Pierce 5 ml columns with Ni-NTA agarose beads (Qiagen) at 4 °C for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from a pool of 20 mechanically homogenized embryos at 96 hpf for each sample (n=4) using TRItidy G (AppliChem) and 500 ng of cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Amplified DNA (2ul from a 1:20 dilution of a 16 h REPLI-g Mini Kit whole-genome amplification, Qiagen) from each sorted population was tested in a 96-well plate in duplicate according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... 1 g of ground leaf powder was resuspended in lysis buffer with 1 mg/ml of proteinase K (19133, Qiagen) and 190 μg/ml of RNase A (19101 ...
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bioRxiv - Developmental Biology 2022Quote: Cells were pelleted by centrifugation after isolation (500 g for 10 min) and subsequently lysed for RNA isolation using RNeasy Micro Kit (Qiagen). For some assays ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted from entire individuals with a salting out method57 and amplified using the REPLI-g Mini Kit (QIAGEN) according to the manufacturer’s protocol with volume modifications (Supplementary Tab ...
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bioRxiv - Cancer Biology 2022Quote: ... Remaining circular DNA was amplified by Multiple Displacement Amplification using φ29 DNA polymerase and random hexamer primers using the Qiagen REPLI-g Mini Kit (Qiagen). Magnetic bead-based purification was used to remove the polymerase and primers ...
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bioRxiv - Genomics 2022Quote: ... the genomic DNA of the Nichi01 strain was isolated from the silk glands of two male fifth-instar larvae using Genomic-tips 100/g and Genomic DNA Buffer Set (QIAGEN). Long-read sequencing for genome assembly was performed using continuous long-read (CLR ...
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bioRxiv - Microbiology 2022Quote: ... The ssDNA genome of the ICBM5 phage was converted to dsDNA by using the REPLI-g Mini kit (Qiagen, Netherlands) and then sequenced with Illumina (paired-end technology 2×300 bp ...
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bioRxiv - Microbiology 2022Quote: ... the beads were suspended in Buffer D2 (denaturation buffer) and subjected to MDA reaction using a REPLI-g Single Cell Kit (QIAGEN).
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bioRxiv - Microbiology 2022Quote: Cyanobacterial cells were collected by centrifugation of liquid cultures at 10000 g for 5 min and DNA was extracted from cell pellets with the DNeasy PowerBiofilm kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Total DNA and RNA were extracted from ~1.5 g of rhizosphere soil using the RNA PowerSoil Kit with the DNA elution kit (Qiagen, Germany). DNA and RNA were extracted using ~0.03 g of roots using the DNeasy Plant DNA Extraction Kit ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA (gDNA) was extracted from stationary broth culture using genomic DNA-Tip 100/G system and buffer set (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... freshly sorted cells were pelleted at 500 g for 10 minutes at 4° and then resuspended in RLT Plus buffer (Qiagen). Cells were vortexed and frozen for at least one day at −80° before being thawed on ice and processed for RNA using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: Total DNA for SCS_HM2 sediments (∼0.5 g) were extracted using the PowerSoil DNA Isolation Kit (Catalog No. 12888-50, Qiagen, Germantown, MD) following the user’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the lysate was spun out of the Feather cartridge at 4000x g and added 100 µL of Ni-NTA agarose resin (Qiagen) to capture HIS-tagged proteins ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining cells were pelleted at 1000 x g for 10 minutes at 4°C and lysed in 350 µL of Buffer RLT (Qiagen) supplemented with 3.5 µL of 2-β mercaptoethanol (#444203 ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from enrichment material in technical duplicates (1 ml digestate or 0.25 g soil) using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) with modified bead beating using a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
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bioRxiv - Molecular Biology 2023Quote: ... MDA controls were performed employing a random-primers-based MDA (RP-MDA) kit as random-primer-based method: Repli-g Mini Kit from QIAGEN; and a TruePrime-based-MDA (PrimPol-MDA ...
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bioRxiv - Evolutionary Biology 2023Quote: High-molecular weight DNA was extracted from podetia of Noboribetsu samples using buffers in DNeasy Blood & Tissue Kit and Genomic-tip 20/G (QIAGEN) with lysis time extended to 5 hours ...
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bioRxiv - Genomics 2023Quote: ... A second DNA library was produced using 2.5 µL native DNA from the same sample after whole genome amplification procedure following the REPLI-G® (Qiagen) manufacturer’s recommendation.
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1.5 h in 50 mL conical tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then collected by centrifugation at 4000 x g for 20 minutes and purified using Ni–NTA–agarose (Qiagen) using standard conditions ...